Restriction map for
pFN21AE4524
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
MaeI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 42 CGCG CG^CG
AflIII 1331 ACATGT A^C[GA][CT]GT
Alw44I 258 GTGCAC G^TGCAC
AocI 186 CCTGAGG CC^T[ACGT]AGG
ApaI 1427 GGGCCC GGGCC^C
ApaLI 258 GTGCAC G^TGCAC
AspI 1222 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 413 GGATCC G^GATCC
BlpI 793 GCTGAGC GC^T[ACGT]AGC
BpmI 1316 CTGGAG CTGGAG(16/14)
Bpu1102I 793 GCTGAGC GC^T[ACGT]AGC
BsmBI 886 GAGACG CGTCTC(1/5)
BsmI 234 GAATGC GAATGC(1/-1)
BspLU11I 1331 ACATGT A^CATGT
BsrFI 399 GCCGGT [GA]^CCGG[CT]
BstUI 42 CGCG CG^CG
BstXI 143 CCATTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 186 CCTGAGG CC^T[ACGT]AGG
CelII 793 GCTGAGC GC^T[ACGT]AGC
CfoI 594 GCGC GCG^C
Cfr10I 399 GCCGGT [GA]^CCGG[CT]
DraI 1437 TTTAAA TTT^AAA
Eco81I 186 CCTGAGG CC^T[ACGT]AGG
EcoNI 573 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 594 GCGC GCG^C
HinP1I 594 GCGC G^CGC
HincII 944 GTCAAC GT[CT]^[GA]AC
HindII 944 GTCAAC GT[CT]^[GA]AC
KspI 41 CCGCGG CCGC^GG
MaeII 485 ACGT A^CGT
MvnI 42 CGCG CG^CG
NcoI 217 CCATGG C^CATGG
NspI 1331 ACATGT [GA]CATG^[CT]
PflFI 1222 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 41 CCGCGG CCGC^GG
SexAI 578 ACCAGGT A^CC[AT]GGT
SmlI 328 CTCAAG C^T[CT][GA]AG
SspI 358 AATATT AAT^ATT
Tth111I 1222 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 635 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1606 CAGAAGCTG
BanI 1447 GGTGCC G^G[CT][GA]CC
1585 GGTGCC
BglI 1539 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1651 GCCAAGAAGGC
BsaI 929 GAGACC GGTCTC(1/5)
1394 GAGACC
BsmFI 1030 GTCCC GGGAC(10/14)
1279 GTCCC
BstEII 495 GGTGACC G^GT[ACGT]ACC
568 GGTCACC
BstPI 495 GGTGACC G^GT[ACGT]ACC
568 GGTCACC
BstYI 267 AGATCC [GA]^GATC[CT]
413 GGATCC
DraII 1426 GGGGCCC [GA]G^G[ACGT]CC[CT]
1427 GGGCCCC
DrdI 1125 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1219 GACGACACTGTC
EcoO109I 1426 GGGGCCC [GA]G^G[ACGT]CC[CT]
1427 GGGCCCC
EcoO65I 495 GGTGACC G^GT[ACGT]ACC
568 GGTCACC
FauI 277 GCGGG CCCGC(4/6)
664 GCGGG
HindIII 331 AAGCTT A^AGCTT
1356 AAGCTT
MflI 267 AGATCC [GA]^GATC[CT]
413 GGATCC
MseI 1049 TTAA T^TAA
1438 TTAA
MspA1I 41 CCGCGG C[AC]G^C[GT]G
662 CAGCGG
PleI 658 GAGTC GAGTC(4/5)
942 GAGTC
PstI 209 CTGCAG CTGCA^G
1576 CTGCAG
SacI 877 GAGCTC GAGCT^C
1408 GAGCTC
SapI 997 GAAGAGC GCTCTTC(1/4)
1410 GCTCTTC
SfcI 209 CTGCAG C^T[GA][CT]AG
1576 CTGCAG
Tru9I 1049 TTAA T^TAA
1438 TTAA
XcmI 1305 CCACAGAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1652 CCAAGAAGGCAGTGG
XhoII 267 AGATCC [GA]^GATC[CT]
413 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BalI
BanII BbsI BbvI BcnI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI TseI Tsp45I Tsp509I
TspRI XmnI