(Circular) (Six-base) MAPSORT of: pFN21AE4524.seq Check: 5774 from: 1 to: 6403 pFN21AE4524 6403 bp With 182 enzymes: SgfI * March 19, 2012 14:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4213 278 Size: 53 83 186 936 2677 2468 Fragments arranged by size: 2677 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3746 1937 Size: 1809 4594 Acc65I G'GTAC_C Cuts at: 3724 3724 Size: 6403 AclI AA'CG_TT Cuts at: 1563 4530 4903 1563 Size: 2967 373 3063 Fragments arranged by size: 3063 2967 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2364 2521 3021 3638 4466 5850 6118 1632 Size: 732 157 500 617 828 1384 268 1917 Fragments arranged by size: 1917 1384 828 732 617 500 268 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6206 AflIII A'CryG_T Cuts at: 1203 3336 5302 6279 1203 Size: 2133 1966 977 1327 Fragments arranged by size: 2133 1966 1327 977 AgeI A'CCGG_T Cuts at: 5234 5234 Size: 6403 AhdI GACnn_n'nnGTC Cuts at: 5132 5132 Size: 6403 AleI CACnn'nnGTG Cuts at: 1166 3145 1166 Size: 1979 4424 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3606 1184 Size: 2422 3981 AlwNI CAG_nnn'CTG Cuts at: 2645 3616 4146 5718 2645 Size: 971 530 1572 3330 Fragments arranged by size: 3330 1572 971 530 ApaI G_GGCC'C Cuts at: 3436 3436 Size: 6403 ApaLI G'TGCA_C Cuts at: 2263 4460 5616 2263 Size: 2197 1156 3050 Fragments arranged by size: 3050 2197 1156 ApoI r'AATT_y Cuts at: 1493 2909 3022 3494 3712 3938 4090 6199 1493 Size: 1416 113 472 218 226 152 2109 1697 Fragments arranged by size: 2109 1697 1416 472 226 218 152 113 AseI AT'TA_AT Cuts at: 160 1032 4957 160 Size: 872 3925 1606 Fragments arranged by size: 3925 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6403 AvaI C'yCGr_G Cuts at: 1870 1943 3728 1870 Size: 73 1785 4545 Fragments arranged by size: 4545 1785 73 BamHI G'GATC_C Cuts at: 2418 3733 4100 2418 Size: 1315 367 4721 Fragments arranged by size: 4721 1315 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3452 3590 3724 5179 618 Size: 325 217 627 1665 138 134 1455 1842 Fragments arranged by size: 1842 1665 1455 627 325 217 138 134 BanII G_rGCy'C Cuts at: 1391 2886 3417 3436 3722 1391 Size: 1495 531 19 286 4072 Fragments arranged by size: 4072 1495 531 286 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2352 2876 3134 928 Size: 971 453 524 258 4197 Fragments arranged by size: 4197 971 524 453 258 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4594 4628 1903 Size: 34 2657 34 3678 Fragments arranged by size: 3678 2657 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4295 5505 4295 Size: 1210 5193 BclI T'GATC_A Cuts at: 1548 1548 Size: 6403 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3550 3662 5014 Size: 107 122 71 631 605 1878 112 1352 Cuts at: 5014 136 Size: 1525 Fragments arranged by size: 1878 1525 1352 631 605 122 112 107 71 BglII A'GATC_T Cuts at: 6398 6398 Size: 6403 BlpI GC'TnA_GC Cuts at: 2799 3820 2799 Size: 1021 5382 Bme1580I G_kGCm'C Cuts at: 1790 2267 2966 3436 4464 5620 1790 Size: 477 699 470 1028 1156 2573 Fragments arranged by size: 2573 1156 1028 699 477 470 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6403 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2578 4134 5092 Size: 416 53 83 399 24 1161 1556 958 Cuts at: 5092 442 Size: 1753 Fragments arranged by size: 1753 1556 1161 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6403 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2217 3436 3472 2217 Size: 1219 36 5148 Fragments arranged by size: 5148 1219 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3342 5063 1239 Size: 2103 1721 2579 Fragments arranged by size: 2579 2103 1721 Bpu10I CC'TnA_GC Cuts at: 3177 3177 Size: 6403 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2318 4528 5393 5691 2318 Size: 2210 865 298 3030 Fragments arranged by size: 3030 2210 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2928 3393 5066 882 Size: 617 1429 465 1673 2219 Fragments arranged by size: 2219 1673 1429 617 465 BsaAI yAC'GTr Cuts at: 493 1204 4194 493 Size: 711 2990 2702 Fragments arranged by size: 2990 2702 711 BsaBI GATnn'nnATC Cuts at: 6397 6397 Size: 6403 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4210 4592 5227 275 Size: 53 83 186 936 2677 382 635 1451 Fragments arranged by size: 2677 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4836 5234 5508 5655 6066 4836 Size: 398 274 147 411 5173 Fragments arranged by size: 5173 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2667 2697 1178 Size: 30 165 30 1264 30 4884 Fragments arranged by size: 4884 1264 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2688 2894 3479 1186 Size: 1502 206 585 4110 Fragments arranged by size: 4110 1502 585 206 BseYI C'CCAG_C Cuts at: 2104 2601 2819 5606 6102 2104 Size: 497 218 2787 496 2405 Fragments arranged by size: 2787 2405 497 496 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5830 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3853 4614 5642 1540 Size: 275 186 1852 761 1028 2301 Fragments arranged by size: 2301 1852 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2267 2886 3328 3417 4464 4549 5620 2267 Size: 619 442 89 1047 85 1071 3050 Fragments arranged by size: 3050 1071 1047 619 442 89 85 BsmI GAATG_Cn' Cuts at: 2245 3914 4007 2245 Size: 1669 93 4641 Fragments arranged by size: 4641 1669 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2885 2885 Size: 6403 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2267 2886 2966 3328 3417 3436 3722 Size: 399 477 619 80 362 89 19 286 Cuts at: 3722 4464 4549 5620 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 619 477 399 362 286 89 85 80 19 BspEI T'CCGG_A Cuts at: 6066 6066 Size: 6403 BspHI T'CATG_A Cuts at: 4292 6022 4292 Size: 1730 4673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3758 844 Size: 2914 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4290 1394 Size: 429 2467 3507 Fragments arranged by size: 3507 2467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2616 3171 3523 4898 5072 65 Size: 1183 1368 555 352 1375 174 1396 Fragments arranged by size: 1396 1375 1368 1183 555 352 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2404 5047 5234 1700 Size: 110 594 2643 187 2869 Fragments arranged by size: 2869 2643 594 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6403 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6403 BssSI C'ACGA_G Cuts at: 4463 5475 4463 Size: 1012 5391 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6403 BstBI TT'CG_AA Cuts at: 5207 5207 Size: 6403 BstEII G'GTnAC_C Cuts at: 1184 2500 2573 1184 Size: 1316 73 5014 Fragments arranged by size: 5014 1316 73 BstXI CCAn_nnnn'nTGG Cuts at: 2155 2155 Size: 6403 BstYI r'GATC_y Cuts at: 1972 2272 2418 3733 4100 4485 4502 5954 6040 Size: 300 146 1315 367 385 17 1452 86 Cuts at: 6040 6052 6398 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1315 385 367 346 300 146 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2192 2192 Size: 6403 BtgI C'CryG_G Cuts at: 513 1065 2046 2222 2422 3403 513 Size: 552 981 176 200 981 3513 Fragments arranged by size: 3513 981 981 552 200 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5664 Fragments arranged by size: 5664 683 56 BtsI GCAGTG_nn' Cuts at: 2814 2854 3672 3919 4713 4733 2814 Size: 40 818 247 794 20 4484 Fragments arranged by size: 4484 818 794 247 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6403 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6368 DraI TTT'AAA Cuts at: 3444 3707 3444 Size: 263 6140 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3136 3230 5410 809 Size: 507 1820 94 2180 1802 Fragments arranged by size: 2180 1820 1802 507 94 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2145 2709 Size: 54 1425 50 275 18 198 117 564 Cuts at: 2709 3551 3850 4739 6368 8 Size: 842 299 889 1629 43 Fragments arranged by size: 1629 1425 889 842 564 299 275 198 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3850 1537 Size: 275 2038 4090 Fragments arranged by size: 4090 2038 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2726 2871 2997 3422 3456 4333 1353 Size: 1373 145 126 425 34 877 3423 Fragments arranged by size: 3423 1373 877 425 145 126 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4990 5364 5510 387 Size: 1064 138 3401 374 146 1280 Fragments arranged by size: 3401 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2884 3415 2884 Size: 531 5872 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2364 2521 3021 3342 3638 4466 5063 Size: 393 732 157 500 321 296 828 597 Cuts at: 5063 5850 6118 1239 Size: 787 268 1524 Fragments arranged by size: 1524 828 787 732 597 500 393 321 296 268 157 EcoNI CCTnn'n_nnAGG Cuts at: 2582 2582 Size: 6403 EcoO109I rG'GnC_Cy Cuts at: 3432 3433 3432 Size: 1 6402 EcoRI G'AATT_C Cuts at: 3712 4090 3712 Size: 378 6025 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2369 2737 2369 Size: 368 6035 FspI TGC'GCA Cuts at: 4909 4909 Size: 6403 HaeII r_GCGC'y Cuts at: 1678 5550 1678 Size: 3872 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2667 2699 3082 3114 5044 Size: 32 135 32 1095 32 383 32 1930 Cuts at: 5044 5076 5118 5150 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1930 1095 383 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2951 3747 3993 669 Size: 988 281 1013 796 246 3079 Fragments arranged by size: 3079 1013 988 796 281 246 HindIII A'AGCT_T Cuts at: 748 2336 3361 748 Size: 1588 1025 3790 Fragments arranged by size: 3790 1588 1025 HpaI GTT'AAC Cuts at: 3993 3993 Size: 6403 KpnI G_GTAC'C Cuts at: 3728 3728 Size: 6403 MfeI C'AATT_G Cuts at: 4002 4002 Size: 6403 MluI A'CGCG_T Cuts at: 6279 6279 Size: 6403 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3028 5517 5701 1305 Size: 1723 2489 184 2007 Fragments arranged by size: 2489 2007 1723 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 2147 2711 3553 10 Size: 54 1425 343 198 117 564 842 2860 Fragments arranged by size: 2860 1425 842 564 343 198 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 3145 3315 3335 3408 4361 4720 4879 Size: 648 1979 170 20 73 953 359 159 Cuts at: 4879 518 Size: 2042 Fragments arranged by size: 2042 1979 953 648 359 170 159 73 20 MspA1I CmG'CkG Cuts at: 1606 2048 2669 3819 4496 5644 5889 1606 Size: 442 621 1150 677 1148 245 2120 Fragments arranged by size: 2120 1150 1148 677 621 442 245 NcoI C'CATG_G Cuts at: 513 1065 2222 513 Size: 552 1157 4694 Fragments arranged by size: 4694 1157 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6403 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6403 NotI GC'GGCC_GC Cuts at: 3850 3850 Size: 6403 NspI r_CATG'y Cuts at: 1132 1229 3340 3761 5306 1132 Size: 97 2111 421 1545 2229 Fragments arranged by size: 2229 2111 1545 421 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6403 PciI A'CATG_T Cuts at: 3336 5302 3336 Size: 1966 4437 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6403 PfoI T'CCnGG_A Cuts at: 2607 3252 2607 Size: 645 5758 PmeI GTTT'AAAC Cuts at: 3707 3707 Size: 6403 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2627 2659 4472 4504 5985 6017 2627 Size: 32 1813 32 1481 32 3013 Fragments arranged by size: 3013 1813 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6403 PsiI TTA'TAA Cuts at: 3973 3973 Size: 6403 PspOMI G'GGCC_C Cuts at: 3432 3432 Size: 6403 PstI C_TGCA'G Cuts at: 830 2218 3585 3755 830 Size: 1388 1367 170 3478 Fragments arranged by size: 3478 1388 1367 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6403 SacI G_AGCT'C Cuts at: 2886 3417 2886 Size: 531 5872 SacII CC_GC'GG Cuts at: 2049 2049 Size: 6403 SalI G'TCGA_C Cuts at: 1936 3745 1936 Size: 1809 4594 SapI GCTCTTCn'nnn_ Cuts at: 2997 3422 2997 Size: 425 5978 SbfI CC_TGCA'GG Cuts at: 3755 3755 Size: 6403 ScaI AGT'ACT Cuts at: 4651 6363 4651 Size: 1712 4691 SexAI A'CCwGG_T Cuts at: 2583 2583 Size: 6403 SfcI C'TryA_G Cuts at: 826 1045 2214 3581 3751 4886 5567 5758 826 Size: 219 1169 1367 170 1135 681 191 1471 Fragments arranged by size: 1471 1367 1169 1135 681 219 191 170 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6403 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6403 SmaI CCC'GGG Cuts at: 1872 3730 1872 Size: 1858 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2333 4507 5408 5670 820 Size: 197 926 390 2174 901 262 1553 Fragments arranged by size: 2174 1553 926 901 390 262 197 SnaBI TAC'GTA Cuts at: 493 4194 493 Size: 3701 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6403 SphI G_CATG'C Cuts at: 1132 3761 1132 Size: 2629 3774 SspI AAT'ATT Cuts at: 5 52 2365 4327 5 Size: 47 2313 1962 2081 Fragments arranged by size: 2313 2081 1962 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6403 StyI C'CwwG_G Cuts at: 513 1065 2222 2277 2793 3842 513 Size: 552 1157 55 516 1049 3074 Fragments arranged by size: 3074 1157 1049 552 516 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 2432 2811 3343 3614 4430 4447 Size: 1255 702 150 379 532 271 816 17 Cuts at: 4447 4600 4785 5230 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 816 702 532 445 379 271 185 153 150 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3558 4649 6361 Size: 274 80 33 51 1443 1581 1091 1712 Cuts at: 6361 96 Size: 138 Fragments arranged by size: 1712 1581 1443 1091 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2257 2289 6363 6395 2257 Size: 32 4074 32 2265 Fragments arranged by size: 4074 2265 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3230 1316 Size: 1914 4489 XbaI T'CTAG_A Cuts at: 3739 3739 Size: 6403 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3317 3664 3317 Size: 347 6056 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6403 XmaI C'CCGG_G Cuts at: 1870 3728 1870 Size: 1858 4545 XmnI GAAnn'nnTTC Cuts at: 2720 3006 3186 4532 2720 Size: 286 180 1346 4591 Fragments arranged by size: 4591 1346 286 180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4211 276 Size: 53 83 186 936 2677 2468 Fragments arranged by size: 2677 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SfiI SfoI SrfI SwaI