(Linear) (Six-base) MAPSORT of: KIEE4524.seq Check: 860 from: 1 to: 1698 KIEE4524.orf With 182 enzymes: SgfI * March 19, 2012 13:10 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 359 516 1016 1633 1698 Size: 359 157 500 617 65 Fragments arranged by size: 617 500 359 157 65 AflIII A'CryG_T Cuts at: 0 1331 1698 Size: 1331 367 AleI CACnn'nnGTG Cuts at: 0 1140 1698 Size: 1140 558 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1601 1698 Size: 1601 97 AlwNI CAG_nnn'CTG Cuts at: 0 640 1611 1698 Size: 640 971 87 Fragments arranged by size: 971 640 87 ApaI G_GGCC'C Cuts at: 0 1431 1698 Size: 1431 267 ApaLI G'TGCA_C Cuts at: 0 258 1698 Size: 258 1440 ApoI r'AATT_y Cuts at: 0 904 1017 1489 1698 Size: 904 113 472 209 Fragments arranged by size: 904 472 209 113 BamHI G'GATC_C Cuts at: 0 413 1698 Size: 413 1285 BanI G'GyrC_C Cuts at: 0 1447 1585 1698 Size: 1447 138 113 Fragments arranged by size: 1447 138 113 BanII G_rGCy'C Cuts at: 0 881 1412 1431 1698 Size: 881 531 19 267 Fragments arranged by size: 881 531 267 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 347 871 1129 1698 Size: 347 524 258 569 Fragments arranged by size: 569 524 347 258 BglI GCCn_nnn'nGGC Cuts at: 0 1545 1657 1698 Size: 1545 112 41 Fragments arranged by size: 1545 112 41 BlpI GC'TnA_GC Cuts at: 0 794 1698 Size: 794 904 Bme1580I G_kGCm'C Cuts at: 0 262 961 1431 1698 Size: 262 699 470 267 Fragments arranged by size: 699 470 267 262 BmrI ACTGGGnnnn_n' Cuts at: 0 573 1698 Size: 573 1125 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 212 1431 1467 1698 Size: 212 1219 36 231 Fragments arranged by size: 1219 231 212 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1337 1698 Size: 1337 361 Bpu10I CC'TnA_GC Cuts at: 0 1172 1698 Size: 1172 526 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 1698 Size: 313 1385 BsaI GGTCTCn'nnnn_ Cuts at: 0 923 1388 1698 Size: 923 465 310 Fragments arranged by size: 923 465 310 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 662 692 1698 Size: 662 30 1006 Fragments arranged by size: 1006 662 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 683 889 1474 1698 Size: 683 206 585 224 Fragments arranged by size: 683 585 224 206 BseYI C'CCAG_C Cuts at: 0 99 596 814 1698 Size: 99 497 218 884 Fragments arranged by size: 884 497 218 99 BsiHKAI G_wGCw'C Cuts at: 0 262 881 1323 1412 1698 Size: 262 619 442 89 286 Fragments arranged by size: 619 442 286 262 89 BsmI GAATG_Cn' Cuts at: 0 240 1698 Size: 240 1458 BsmBI CGTCTCn'nnnn_ Cuts at: 0 880 1698 Size: 880 818 Bsp1286I G_dGCh'C Cuts at: 0 262 881 961 1323 1412 1431 1698 Size: 262 619 80 362 89 19 267 Fragments arranged by size: 619 362 267 262 89 80 19 BsrDI GCAATG_nn' Cuts at: 0 611 1166 1518 1698 Size: 611 555 352 180 Fragments arranged by size: 611 555 352 180 BsrFI r'CCGG_y Cuts at: 0 399 1698 Size: 399 1299 BstEII G'GTnAC_C Cuts at: 0 495 568 1698 Size: 495 73 1130 Fragments arranged by size: 1130 495 73 BstXI CCAn_nnnn'nTGG Cuts at: 0 150 1698 Size: 150 1548 BstYI r'GATC_y Cuts at: 0 267 413 1698 Size: 267 146 1285 Fragments arranged by size: 1285 267 146 Bsu36I CC'TnA_GG Cuts at: 0 187 1698 Size: 187 1511 BtgI C'CryG_G Cuts at: 0 41 217 417 1398 1698 Size: 41 176 200 981 300 Fragments arranged by size: 981 300 200 176 41 BtsI GCAGTG_nn' Cuts at: 0 809 849 1667 1698 Size: 809 40 818 31 Fragments arranged by size: 818 809 40 31 DraI TTT'AAA Cuts at: 0 1439 1698 Size: 1439 259 DrdI GACnn_nn'nnGTC Cuts at: 0 1131 1225 1698 Size: 1131 94 473 Fragments arranged by size: 1131 473 94 EaeI y'GGCC_r Cuts at: 0 23 140 704 1546 1698 Size: 23 117 564 842 152 Fragments arranged by size: 842 564 152 117 23 EarI CTCTTCn'nnn_ Cuts at: 0 721 866 992 1417 1451 1698 Size: 721 145 126 425 34 247 Fragments arranged by size: 721 425 247 145 126 34 EcoICRI GAG'CTC Cuts at: 0 879 1410 1698 Size: 879 531 288 Fragments arranged by size: 879 531 288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 359 516 1016 1337 1633 1698 Size: 359 157 500 321 296 65 Fragments arranged by size: 500 359 321 296 157 65 EcoNI CCTnn'n_nnAGG Cuts at: 0 577 1698 Size: 577 1121 EcoO109I rG'GnC_Cy Cuts at: 0 1427 1428 1698 Size: 1427 1 270 Fragments arranged by size: 1427 270 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 364 732 1698 Size: 364 368 966 Fragments arranged by size: 966 368 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 662 694 1077 1109 1698 Size: 662 32 383 32 589 Fragments arranged by size: 662 589 383 32 32 HincII GTy'rAC Cuts at: 0 946 1698 Size: 946 752 HindIII A'AGCT_T Cuts at: 0 331 1356 1698 Size: 331 1025 342 Fragments arranged by size: 1025 342 331 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1023 1698 Size: 1023 675 MscI TGG'CCA Cuts at: 0 25 142 706 1548 1698 Size: 25 117 564 842 150 Fragments arranged by size: 842 564 150 117 25 MslI CAynn'nnrTG Cuts at: 0 1140 1310 1330 1403 1698 Size: 1140 170 20 73 295 Fragments arranged by size: 1140 295 170 73 20 MspA1I CmG'CkG Cuts at: 0 43 664 1698 Size: 43 621 1034 Fragments arranged by size: 1034 621 43 NcoI C'CATG_G Cuts at: 0 217 1698 Size: 217 1481 NspI r_CATG'y Cuts at: 0 1335 1698 Size: 1335 363 PciI A'CATG_T Cuts at: 0 1331 1698 Size: 1331 367 PfoI T'CCnGG_A Cuts at: 0 602 1247 1698 Size: 602 645 451 Fragments arranged by size: 645 602 451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 622 654 1698 Size: 622 32 1044 Fragments arranged by size: 1044 622 32 PspOMI G'GGCC_C Cuts at: 0 1427 1698 Size: 1427 271 PstI C_TGCA'G Cuts at: 0 213 1580 1698 Size: 213 1367 118 Fragments arranged by size: 1367 213 118 SacI G_AGCT'C Cuts at: 0 881 1412 1698 Size: 881 531 286 Fragments arranged by size: 881 531 286 SacII CC_GC'GG Cuts at: 0 44 1698 Size: 44 1654 SapI GCTCTTCn'nnn_ Cuts at: 0 992 1417 1698 Size: 992 425 281 Fragments arranged by size: 992 425 281 SexAI A'CCwGG_T Cuts at: 0 578 1698 Size: 578 1120 SfcI C'TryA_G Cuts at: 0 209 1576 1698 Size: 209 1367 122 Fragments arranged by size: 1367 209 122 SmlI C'TyrA_G Cuts at: 0 328 1698 Size: 328 1370 SspI AAT'ATT Cuts at: 0 360 1698 Size: 360 1338 StyI C'CwwG_G Cuts at: 0 217 272 788 1698 Size: 217 55 516 910 Fragments arranged by size: 910 516 217 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 277 427 806 1338 1609 1698 Size: 277 150 379 532 271 89 Fragments arranged by size: 532 379 277 271 150 89 TatI w'GTAC_w Cuts at: 0 1553 1698 Size: 1553 145 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 252 284 1698 Size: 252 32 1414 Fragments arranged by size: 1414 252 32 Tth111I GACn'n_nGTC Cuts at: 0 1225 1698 Size: 1225 473 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1312 1659 1698 Size: 1312 347 39 Fragments arranged by size: 1312 347 39 XmnI GAAnn'nnTTC Cuts at: 0 715 1001 1181 1698 Size: 715 286 180 517 Fragments arranged by size: 715 517 286 180 Enzymes that do cut: AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbsI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NspI PciI PfoI PpiI PspOMI PstI SacI SacII SapI SexAI SfcI SmlI SspI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraIII EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XhoI XmaI ZraI