(Circular) (Six-base) MAPSORT of: pFN21AE3588.seq Check: 6200 from: 1 to: 7063 pFN21AE3588 7063 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4095 4873 278 Size: 53 83 186 936 2559 778 2468 Fragments arranged by size: 2559 2468 936 778 186 83 53 AccI GT'mk_AC Cuts at: 1937 3927 4406 1937 Size: 1990 479 4594 Fragments arranged by size: 4594 1990 479 Acc65I G'GTAC_C Cuts at: 3024 4384 3024 Size: 1360 5703 AclI AA'CG_TT Cuts at: 1563 5190 5563 1563 Size: 3627 373 3063 Fragments arranged by size: 3627 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2288 2392 2411 5126 6510 6778 1632 Size: 578 78 104 19 2715 1384 268 1917 Fragments arranged by size: 2715 1917 1384 578 268 104 78 19 AfeI AGC'GCT Cuts at: 3566 3566 Size: 7063 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6866 AflIII A'CryG_T Cuts at: 1203 5962 6939 1203 Size: 4759 977 1327 Fragments arranged by size: 4759 1327 977 AgeI A'CCGG_T Cuts at: 3021 5894 3021 Size: 2873 4190 AhdI GACnn_n'nnGTC Cuts at: 5792 5792 Size: 7063 AleI CACnn'nnGTG Cuts at: 1166 2214 3233 1166 Size: 1048 1019 4996 Fragments arranged by size: 4996 1048 1019 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7063 AlwNI CAG_nnn'CTG Cuts at: 2245 2714 3067 3292 4015 4806 6378 2245 Size: 469 353 225 723 791 1572 2930 Fragments arranged by size: 2930 1572 791 723 469 353 225 ApaI G_GGCC'C Cuts at: 4165 4165 Size: 7063 ApaLI G'TGCA_C Cuts at: 5120 6276 5120 Size: 1156 5907 ApoI r'AATT_y Cuts at: 1493 2299 4372 4598 4750 6859 1493 Size: 806 2073 226 152 2109 1697 Fragments arranged by size: 2109 2073 1697 806 226 152 AseI AT'TA_AT Cuts at: 160 1032 5617 160 Size: 872 4585 1606 Fragments arranged by size: 4585 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7063 AvaI C'yCGr_G Cuts at: 1870 1943 3700 4388 1870 Size: 73 1757 688 4545 Fragments arranged by size: 4545 1757 688 73 BamHI G'GATC_C Cuts at: 4393 4760 4393 Size: 367 6696 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2435 3024 3043 4384 5839 Size: 325 217 627 648 589 19 1341 1455 Cuts at: 5839 618 Size: 1842 Fragments arranged by size: 1842 1455 1341 648 627 589 325 217 19 BanII G_rGCy'C Cuts at: 1391 3315 4165 4382 1391 Size: 1924 850 217 4072 Fragments arranged by size: 4072 1924 850 217 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2890 3459 3750 928 Size: 971 991 569 291 4241 Fragments arranged by size: 4241 991 971 569 291 BbvCI CC'TCA_GC Cuts at: 3662 3689 3662 Size: 27 7036 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 5254 5288 1903 Size: 34 1862 34 1421 34 3678 Fragments arranged by size: 3678 1862 1421 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4955 6165 4955 Size: 1210 5853 BclI T'GATC_A Cuts at: 1548 1548 Size: 7063 BfrBI ATG'CAT Cuts at: 4317 4317 Size: 7063 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3359 5674 136 Size: 107 122 71 631 605 1687 2315 1525 Fragments arranged by size: 2315 1687 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7058 7058 Size: 7063 BlpI GC'TnA_GC Cuts at: 2418 3596 4480 2418 Size: 1178 884 5001 Fragments arranged by size: 5001 1178 884 Bme1580I G_kGCm'C Cuts at: 1790 4165 5124 6280 1790 Size: 2375 959 1156 2573 Fragments arranged by size: 2573 2375 1156 959 BmgBI CAC'GTC Cuts at: 1318 3889 1318 Size: 2571 4492 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4794 5752 442 Size: 416 53 83 399 24 3377 958 1753 Fragments arranged by size: 3377 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7063 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3214 3214 Size: 7063 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2241 2846 3044 3722 3844 5723 1239 Size: 1002 605 198 678 122 1879 2579 Fragments arranged by size: 2579 1879 1002 678 605 198 122 Bpu10I CC'TnA_GC Cuts at: 3662 3689 3662 Size: 27 7036 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2122 3493 5188 6053 6351 2122 Size: 1371 1695 865 298 2834 Fragments arranged by size: 2834 1695 1371 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5726 882 Size: 617 4227 2219 Fragments arranged by size: 4227 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4854 493 Size: 711 3650 2702 Fragments arranged by size: 3650 2702 711 BsaBI GATnn'nnATC Cuts at: 7057 7057 Size: 7063 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3504 4092 4870 5252 Size: 53 83 186 936 1971 588 778 382 Cuts at: 5252 5887 275 Size: 635 1451 Fragments arranged by size: 1971 1451 936 778 635 588 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3021 3437 5496 5894 6168 6315 6726 3021 Size: 416 2059 398 274 147 411 3358 Fragments arranged by size: 3358 2059 416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6838 Fragments arranged by size: 6838 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 2618 2651 2666 3049 3809 3986 4102 Size: 906 526 33 15 383 760 177 116 Cuts at: 4102 4343 4346 1186 Size: 241 3 3903 Fragments arranged by size: 3903 906 760 526 383 241 177 116 33 15 3 BseYI C'CCAG_C Cuts at: 2184 2320 2769 3067 3285 3349 3805 3899 4287 Size: 136 449 298 218 64 456 94 388 Cuts at: 4287 6266 6762 2184 Size: 1979 496 2485 Fragments arranged by size: 2485 1979 496 456 449 388 298 218 136 94 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2927 2981 3076 3308 3316 1163 Size: 573 1191 54 95 232 8 4910 Fragments arranged by size: 4910 1191 573 232 95 54 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4513 5274 6302 1540 Size: 275 186 2512 761 1028 2301 Fragments arranged by size: 2512 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 3315 5124 5209 6280 2124 Size: 1191 1809 85 1071 2907 Fragments arranged by size: 2907 1809 1191 1071 85 BsmI GAATG_Cn' Cuts at: 4574 4667 4574 Size: 93 6970 BsmBI CGTCTCn'nnnn_ Cuts at: 2259 3202 3791 2259 Size: 943 589 5531 Fragments arranged by size: 5531 943 589 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 3315 4165 4382 5124 5209 6280 Size: 399 334 1191 850 217 742 85 1071 Cuts at: 6280 1391 Size: 2174 Fragments arranged by size: 2174 1191 1071 850 742 399 334 217 85 BspEI T'CCGG_A Cuts at: 6726 6726 Size: 7063 BspHI T'CATG_A Cuts at: 4952 6682 4952 Size: 1730 5333 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3036 4418 844 Size: 2192 1382 3489 Fragments arranged by size: 3489 2192 1382 BsrBI CCG'CTC Cuts at: 1394 1823 2013 2422 2484 4950 1394 Size: 429 190 409 62 2466 3507 Fragments arranged by size: 3507 2466 429 409 190 62 BsrDI GCAATG_nn' Cuts at: 65 1248 3946 5558 5732 65 Size: 1183 2698 1612 174 1396 Fragments arranged by size: 2698 1612 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3021 5707 5894 1700 Size: 110 1211 2686 187 2869 Fragments arranged by size: 2869 2686 1211 187 110 BsrGI T'GTAC_A Cuts at: 96 2176 96 Size: 2080 4983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7063 BssSI C'ACGA_G Cuts at: 2510 5123 6135 2510 Size: 2613 1012 3438 Fragments arranged by size: 3438 2613 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3078 794 Size: 2284 4779 BstBI TT'CG_AA Cuts at: 2304 5867 2304 Size: 3563 3500 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7063 BstXI CCAn_nnnn'nTGG Cuts at: 3235 4060 3235 Size: 825 6238 BstYI r'GATC_y Cuts at: 1972 2710 3114 3675 4032 4393 4760 5145 5162 Size: 738 404 561 357 361 367 385 17 Cuts at: 5162 6614 6700 6712 7058 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 738 561 404 385 367 361 357 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2519 4102 2519 Size: 1583 5480 BtgI C'CryG_G Cuts at: 513 1065 2004 2471 2502 3006 3047 3921 4143 Size: 552 939 467 31 504 41 874 222 Cuts at: 4143 513 Size: 3433 Fragments arranged by size: 3433 939 874 552 504 467 222 41 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2734 3058 487 Size: 683 56 1508 324 4492 Fragments arranged by size: 4492 1508 683 324 56 BtsI GCAGTG_nn' Cuts at: 2566 3771 4285 4579 5373 5393 2566 Size: 1205 514 294 794 20 4236 Fragments arranged by size: 4236 1205 794 514 294 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7063 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4125 4160 529 Size: 35 3561 35 3432 Fragments arranged by size: 3561 3432 35 35 DraI TTT'AAA Cuts at: 4367 4367 Size: 7063 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2704 6070 809 Size: 507 1388 3366 1802 Fragments arranged by size: 3366 1802 1388 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2249 3381 Size: 54 1425 50 275 18 333 86 1132 Cuts at: 3381 4510 5399 7028 8 Size: 1129 889 1629 43 Fragments arranged by size: 1629 1425 1132 1129 889 333 275 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4510 1537 Size: 275 2698 4090 Fragments arranged by size: 4090 2698 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2462 3582 4993 1353 Size: 1109 1120 1411 3423 Fragments arranged by size: 3423 1411 1120 1109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2453 3190 3328 3721 5650 6024 Size: 1064 138 864 737 138 393 1929 374 Cuts at: 6024 6170 387 Size: 146 1280 Fragments arranged by size: 1929 1280 1064 864 737 393 374 146 138 138 EcoICRI GAG'CTC Cuts at: 3313 3313 Size: 7063 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2241 2288 2392 2411 2846 3044 Size: 393 578 31 47 104 19 435 198 Cuts at: 3044 3722 3844 5126 5723 6510 6778 1239 Size: 678 122 1282 597 787 268 1524 Fragments arranged by size: 1524 1282 787 678 597 578 435 393 268 198 122 104 47 31 19 EcoNI CCTnn'n_nnAGG Cuts at: 2633 2633 Size: 7063 EcoO109I rG'GnC_Cy Cuts at: 2082 2194 3169 3250 3430 3995 4003 4161 4162 Size: 112 975 81 180 565 8 158 1 Cuts at: 4162 2082 Size: 4983 Fragments arranged by size: 4983 975 565 180 158 112 81 8 1 EcoRI G'AATT_C Cuts at: 4372 4750 4372 Size: 378 6685 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3508 3508 Size: 7063 FspI TGC'GCA Cuts at: 5569 5569 Size: 7063 HaeII r_GCGC'y Cuts at: 1678 3568 4022 6210 1678 Size: 1890 454 2188 2531 Fragments arranged by size: 2531 2188 1890 454 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3824 3856 4187 4219 5704 Size: 32 135 32 2252 32 331 32 1485 Cuts at: 5704 5736 5778 5810 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2252 1485 331 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4407 4653 669 Size: 988 281 2469 246 3079 Fragments arranged by size: 3079 2469 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7063 HpaI GTT'AAC Cuts at: 4653 4653 Size: 7063 KpnI G_GTAC'C Cuts at: 3028 4388 3028 Size: 1360 5703 MfeI C'AATT_G Cuts at: 4662 4662 Size: 7063 MluI A'CGCG_T Cuts at: 6939 6939 Size: 7063 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6177 6361 1305 Size: 4872 184 2007 Fragments arranged by size: 4872 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3383 10 Size: 54 1425 343 1551 3690 Fragments arranged by size: 3690 1551 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2214 3233 5021 5380 5539 518 Size: 648 1048 1019 1788 359 159 2042 Fragments arranged by size: 2042 1788 1048 1019 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2188 2536 2782 3049 3064 3424 3550 3903 Size: 582 348 246 267 15 360 126 353 Cuts at: 3903 4479 5156 6304 6549 1606 Size: 576 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 582 576 360 353 348 267 246 245 126 15 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5572 Fragments arranged by size: 5572 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7063 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7063 NotI GC'GGCC_GC Cuts at: 4510 4510 Size: 7063 NsiI A_TGCA'T Cuts at: 4319 4319 Size: 7063 NspI r_CATG'y Cuts at: 1132 1229 2229 4421 5966 1132 Size: 97 1000 2192 1545 2229 Fragments arranged by size: 2229 2192 1545 1000 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7063 PciI A'CATG_T Cuts at: 5962 5962 Size: 7063 PflMI CCAn_nnn'nTGG Cuts at: 1108 2220 3067 1108 Size: 1112 847 5104 Fragments arranged by size: 5104 1112 847 PfoI T'CCnGG_A Cuts at: 2238 3126 3738 2238 Size: 888 612 5563 Fragments arranged by size: 5563 888 612 PmeI GTTT'AAAC Cuts at: 4367 4367 Size: 7063 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5132 5164 6645 6677 5132 Size: 32 1481 32 5518 Fragments arranged by size: 5518 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 3169 3250 3430 3995 4003 2194 Size: 975 81 180 565 8 5254 Fragments arranged by size: 5254 975 565 180 81 8 PshAI GACnn'nnGTC Cuts at: 1613 3263 1613 Size: 1650 5413 PsiI TTA'TAA Cuts at: 4633 4633 Size: 7063 PspOMI G'GGCC_C Cuts at: 4161 4161 Size: 7063 PstI C_TGCA'G Cuts at: 830 2541 4415 830 Size: 1711 1874 3478 Fragments arranged by size: 3478 1874 1711 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7063 PvuII CAG'CTG Cuts at: 2188 2536 2782 3064 3424 2188 Size: 348 246 282 360 5827 Fragments arranged by size: 5827 360 348 282 246 SacI G_AGCT'C Cuts at: 3315 3315 Size: 7063 SacII CC_GC'GG Cuts at: 3050 3050 Size: 7063 SalI G'TCGA_C Cuts at: 1936 4405 1936 Size: 2469 4594 SanDI GG'GwC_CC Cuts at: 3995 3995 Size: 7063 SapI GCTCTTCn'nnn_ Cuts at: 2462 2462 Size: 7063 SbfI CC_TGCA'GG Cuts at: 4415 4415 Size: 7063 ScaI AGT'ACT Cuts at: 5311 7023 5311 Size: 1712 5351 SexAI A'CCwGG_T Cuts at: 2196 3171 2196 Size: 975 6088 SfcI C'TryA_G Cuts at: 826 1045 2029 2148 2537 3517 3601 4411 5546 Size: 219 984 119 389 980 84 810 1135 Cuts at: 5546 6227 6418 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 984 980 810 681 389 219 191 119 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7063 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7063 SmaI CCC'GGG Cuts at: 1872 4390 1872 Size: 2518 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2101 3472 5167 6068 6330 820 Size: 197 926 158 1371 1695 901 262 1553 Fragments arranged by size: 1695 1553 1371 926 901 262 197 158 SnaBI TAC'GTA Cuts at: 493 4854 493 Size: 4361 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7063 SphI G_CATG'C Cuts at: 1132 4421 1132 Size: 3289 3774 SspI AAT'ATT Cuts at: 5 52 4987 5 Size: 47 4935 2081 Fragments arranged by size: 4935 2081 47 StuI AGG'CCT Cuts at: 1517 2349 2396 1517 Size: 832 47 6184 Fragments arranged by size: 6184 832 47 StyI C'CwwG_G Cuts at: 513 1065 2004 3204 3510 4502 513 Size: 552 939 1200 306 992 3074 Fragments arranged by size: 3074 1200 992 939 552 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2423 4043 5090 5107 5260 5445 5890 Size: 1255 843 1620 1047 17 153 185 445 Cuts at: 5890 325 Size: 1498 Fragments arranged by size: 1620 1498 1255 1047 843 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2176 5309 7021 Size: 274 80 33 51 1443 199 3133 1712 Cuts at: 7021 96 Size: 138 Fragments arranged by size: 3133 1712 1443 274 199 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2540 2572 2814 2846 4114 4146 7023 7055 2540 Size: 32 242 32 1268 32 2877 32 2548 Fragments arranged by size: 2877 2548 1268 242 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7063 XbaI T'CTAG_A Cuts at: 4399 4399 Size: 7063 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4141 4141 Size: 7063 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7063 XmaI C'CCGG_G Cuts at: 1870 4388 1870 Size: 2518 4545 XmnI GAAnn'nnTTC Cuts at: 2978 3491 5192 2978 Size: 513 1701 4849 Fragments arranged by size: 4849 1701 513 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4093 4871 276 Size: 53 83 186 936 2559 778 2468 Fragments arranged by size: 2559 2468 936 778 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SfiI SfoI SrfI SwaI