(Linear) (Six-base) MAPSORT of: KIEE3588.seq Check: 7188 from: 1 to: 2358 KIEE3588.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AatII G_ACGT'C Cuts at: 0 2090 2358 Size: 2090 268 AccI GT'mk_AC Cuts at: 0 1922 2358 Size: 1922 436 Acc65I G'GTAC_C Cuts at: 0 1019 2358 Size: 1019 1339 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 283 387 406 2358 Size: 205 78 104 19 1952 Fragments arranged by size: 1952 205 104 78 19 AfeI AGC'GCT Cuts at: 0 1561 2358 Size: 1561 797 AgeI A'CCGG_T Cuts at: 0 1016 2358 Size: 1016 1342 AleI CACnn'nnGTG Cuts at: 0 209 1228 2358 Size: 209 1019 1130 Fragments arranged by size: 1130 1019 209 AlwNI CAG_nnn'CTG Cuts at: 0 240 709 1062 1287 2010 2358 Size: 240 469 353 225 723 348 Fragments arranged by size: 723 469 353 348 240 225 ApaI G_GGCC'C Cuts at: 0 2160 2358 Size: 2160 198 ApoI r'AATT_y Cuts at: 0 294 2358 Size: 294 2064 AvaI C'yCGr_G Cuts at: 0 1695 2358 Size: 1695 663 BanI G'GyrC_C Cuts at: 0 430 1019 1038 2358 Size: 430 589 19 1320 Fragments arranged by size: 1320 589 430 19 BanII G_rGCy'C Cuts at: 0 1310 2160 2358 Size: 1310 850 198 Fragments arranged by size: 1310 850 198 BbsI GAAGACnn'nnnn_ Cuts at: 0 885 1454 1745 2358 Size: 885 569 291 613 Fragments arranged by size: 885 613 569 291 BbvCI CC'TCA_GC Cuts at: 0 1657 1684 2358 Size: 1657 27 674 Fragments arranged by size: 1657 674 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1794 1828 2358 Size: 1794 34 530 Fragments arranged by size: 1794 530 34 BfrBI ATG'CAT Cuts at: 0 2312 2358 Size: 2312 46 BglI GCCn_nnn'nGGC Cuts at: 0 1354 2358 Size: 1354 1004 BlpI GC'TnA_GC Cuts at: 0 413 1591 2358 Size: 413 1178 767 Fragments arranged by size: 1178 767 413 Bme1580I G_kGCm'C Cuts at: 0 2160 2358 Size: 2160 198 BmgBI CAC'GTC Cuts at: 0 1884 2358 Size: 1884 474 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1209 2358 Size: 1209 1149 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 841 1039 1717 1839 2358 Size: 236 605 198 678 122 519 Fragments arranged by size: 678 605 519 236 198 122 Bpu10I CC'TnA_GC Cuts at: 0 1657 1684 2358 Size: 1657 27 674 Fragments arranged by size: 1657 674 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 117 1488 2358 Size: 117 1371 870 Fragments arranged by size: 1371 870 117 BsaHI Gr'CG_yC Cuts at: 0 1499 2087 2358 Size: 1499 588 271 Fragments arranged by size: 1499 588 271 BsaWI w'CCGG_w Cuts at: 0 1016 1432 2358 Size: 1016 416 926 Fragments arranged by size: 1016 926 416 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 87 613 646 661 1044 1804 1981 2097 Size: 87 526 33 15 383 760 177 116 Cuts at: 2097 2338 2341 2358 Size: 241 3 17 Fragments arranged by size: 760 526 383 241 177 116 87 33 17 15 3 BseYI C'CCAG_C Cuts at: 0 179 315 764 1062 1280 1344 1800 1894 Size: 179 136 449 298 218 64 456 94 Cuts at: 1894 2282 2358 Size: 388 76 Fragments arranged by size: 456 449 388 298 218 179 136 94 76 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 922 976 1071 1303 1311 2358 Size: 922 54 95 232 8 1047 Fragments arranged by size: 1047 922 232 95 54 8 BsiHKAI G_wGCw'C Cuts at: 0 119 1310 2358 Size: 119 1191 1048 Fragments arranged by size: 1191 1048 119 BsmBI CGTCTCn'nnnn_ Cuts at: 0 254 1197 1786 2358 Size: 254 943 589 572 Fragments arranged by size: 943 589 572 254 Bsp1286I G_dGCh'C Cuts at: 0 119 1310 2160 2358 Size: 119 1191 850 198 Fragments arranged by size: 1191 850 198 119 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1031 2358 Size: 1031 1327 BsrBI CCG'CTC Cuts at: 0 8 417 479 2358 Size: 8 409 62 1879 Fragments arranged by size: 1879 409 62 8 BsrDI GCAATG_nn' Cuts at: 0 1941 2358 Size: 1941 417 BsrFI r'CCGG_y Cuts at: 0 1016 2358 Size: 1016 1342 BsrGI T'GTAC_A Cuts at: 0 171 2358 Size: 171 2187 BssSI C'ACGA_G Cuts at: 0 505 2358 Size: 505 1853 BstAPI GCAn_nnn'nTGC Cuts at: 0 1073 2358 Size: 1073 1285 BstBI TT'CG_AA Cuts at: 0 299 2358 Size: 299 2059 BstXI CCAn_nnnn'nTGG Cuts at: 0 1230 2055 2358 Size: 1230 825 303 Fragments arranged by size: 1230 825 303 BstYI r'GATC_y Cuts at: 0 705 1109 1670 2027 2358 Size: 705 404 561 357 331 Fragments arranged by size: 705 561 404 357 331 Bsu36I CC'TnA_GG Cuts at: 0 514 2097 2358 Size: 514 1583 261 Fragments arranged by size: 1583 514 261 BtgI C'CryG_G Cuts at: 0 466 497 1001 1042 1916 2138 2358 Size: 466 31 504 41 874 222 220 Fragments arranged by size: 874 504 466 222 220 41 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 729 1053 2358 Size: 729 324 1305 Fragments arranged by size: 1305 729 324 BtsI GCAGTG_nn' Cuts at: 0 561 1766 2280 2358 Size: 561 1205 514 78 Fragments arranged by size: 1205 561 514 78 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2120 2155 2358 Size: 2120 35 203 Fragments arranged by size: 2120 203 35 DrdI GACnn_nn'nnGTC Cuts at: 0 699 2358 Size: 699 1659 EaeI y'GGCC_r Cuts at: 0 158 244 1376 2358 Size: 158 86 1132 982 Fragments arranged by size: 1132 982 158 86 EarI CTCTTCn'nnn_ Cuts at: 0 457 1577 2358 Size: 457 1120 781 Fragments arranged by size: 1120 781 457 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 448 1185 1323 1716 2358 Size: 448 737 138 393 642 Fragments arranged by size: 737 642 448 393 138 EcoICRI GAG'CTC Cuts at: 0 1308 2358 Size: 1308 1050 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 236 283 387 406 841 1039 1717 Size: 205 31 47 104 19 435 198 678 Cuts at: 1717 1839 2358 Size: 122 519 Fragments arranged by size: 678 519 435 205 198 122 104 47 31 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 628 2358 Size: 628 1730 EcoO109I rG'GnC_Cy Cuts at: 0 77 189 1164 1245 1425 1990 1998 2156 Size: 77 112 975 81 180 565 8 158 Cuts at: 2156 2157 2358 Size: 1 201 Fragments arranged by size: 975 565 201 180 158 112 81 77 8 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1503 2358 Size: 1503 855 HaeII r_GCGC'y Cuts at: 0 1563 2017 2358 Size: 1563 454 341 Fragments arranged by size: 1563 454 341 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1819 1851 2182 2214 2358 Size: 1819 32 331 32 144 Fragments arranged by size: 1819 331 144 32 32 KpnI G_GTAC'C Cuts at: 0 1023 2358 Size: 1023 1335 MscI TGG'CCA Cuts at: 0 1378 2358 Size: 1378 980 MslI CAynn'nnrTG Cuts at: 0 209 1228 2358 Size: 209 1019 1130 Fragments arranged by size: 1130 1019 209 MspA1I CmG'CkG Cuts at: 0 183 531 777 1044 1059 1419 1545 1898 Size: 183 348 246 267 15 360 126 353 Cuts at: 1898 2358 Size: 460 Fragments arranged by size: 460 360 353 348 267 246 183 126 15 NsiI A_TGCA'T Cuts at: 0 2314 2358 Size: 2314 44 NspI r_CATG'y Cuts at: 0 224 2358 Size: 224 2134 PflMI CCAn_nnn'nTGG Cuts at: 0 215 1062 2358 Size: 215 847 1296 Fragments arranged by size: 1296 847 215 PfoI T'CCnGG_A Cuts at: 0 233 1121 1733 2358 Size: 233 888 612 625 Fragments arranged by size: 888 625 612 233 PpuMI rG'GwC_Cy Cuts at: 0 189 1164 1245 1425 1990 1998 2358 Size: 189 975 81 180 565 8 360 Fragments arranged by size: 975 565 360 189 180 81 8 PshAI GACnn'nnGTC Cuts at: 0 1258 2358 Size: 1258 1100 PspOMI G'GGCC_C Cuts at: 0 2156 2358 Size: 2156 202 PstI C_TGCA'G Cuts at: 0 536 2358 Size: 536 1822 PvuII CAG'CTG Cuts at: 0 183 531 777 1059 1419 2358 Size: 183 348 246 282 360 939 Fragments arranged by size: 939 360 348 282 246 183 SacI G_AGCT'C Cuts at: 0 1310 2358 Size: 1310 1048 SacII CC_GC'GG Cuts at: 0 1045 2358 Size: 1045 1313 SanDI GG'GwC_CC Cuts at: 0 1990 2358 Size: 1990 368 SapI GCTCTTCn'nnn_ Cuts at: 0 457 2358 Size: 457 1901 SexAI A'CCwGG_T Cuts at: 0 191 1166 2358 Size: 191 975 1192 Fragments arranged by size: 1192 975 191 SfcI C'TryA_G Cuts at: 0 24 143 532 1512 1596 2358 Size: 24 119 389 980 84 762 Fragments arranged by size: 980 762 389 119 84 24 SmlI C'TyrA_G Cuts at: 0 96 1467 2358 Size: 96 1371 891 Fragments arranged by size: 1371 891 96 StuI AGG'CCT Cuts at: 0 344 391 2358 Size: 344 47 1967 Fragments arranged by size: 1967 344 47 StyI C'CwwG_G Cuts at: 0 1199 1505 2358 Size: 1199 306 853 Fragments arranged by size: 1199 853 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 418 2038 2358 Size: 418 1620 320 Fragments arranged by size: 1620 418 320 TatI w'GTAC_w Cuts at: 0 171 2358 Size: 171 2187 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 535 567 809 841 2109 2141 2358 Size: 535 32 242 32 1268 32 217 Fragments arranged by size: 1268 535 242 217 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2136 2358 Size: 2136 222 XmnI GAAnn'nnTTC Cuts at: 0 973 1486 2358 Size: 973 513 872 Fragments arranged by size: 973 872 513 ZraI GAC'GTC Cuts at: 0 2088 2358 Size: 2088 270 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AgeI AleI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MscI MslI MspA1I NsiI NspI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SapI SexAI SfcI SmlI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI AclI AflII AflIII AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BglII BmrI BmtI BsaI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI BspEI BspHI BssHII BstEII BstZ17I ClaI DraI DraIII EagI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI