################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 386 CCGC CCGC(-3/-1) Alw44I 348 GTGCAC G^TGCAC ApaI 620 GGGCCC GGGCC^C ApaLI 348 GTGCAC G^TGCAC AspHI 348 GTGCAC G[AT]GC[AT]^C AvaII 38 GGACC G^G[AT]CC BalI 532 TGGCCA TGG^CCA BbsI 269 GTCTTC GAAGAC(2/6) BbvCI 418 CCTCAGC CCTCAGC(-5/-2) BpuAI 269 GTCTTC GAAGAC(2/6) BsaI 204 GGTCTC GGTCTC(1/5) BsaWI 759 ACCGGA [AT]^CCGG[AT] BsiEI 391 CGGTCG CG[GA][CT]^CG BsiHKAI 348 GTGCAC G[AT]GC[AT]^C BsmBI 395 CGTCTC CGTCTC(1/5) BsrBI 386 CCGCTC CCGCTC(-3/-3) BsrDI 320 GCAATG GCAATG(2/0) BssSI 145 CTCGTG CACGAG(-5/-1) BstXI 10 CCACATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 544 AGATCC [GA]^GATC[CT] DraIII 351 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 384 GACCGCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 365 CTCTTC CTCTTC(1/4) EcoNI 144 CCTCGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HphI 608 TCACC GGTGA(8/7) Ksp632I 365 CTCTTC CTCTTC(1/4) MflI 544 AGATCC [GA]^GATC[CT] MluNI 532 TGGCCA TGG^CCA MscI 532 TGGCCA TGG^CCA MslI 573 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 477 CATG CATG^ NspI 476 ACATGC [GA]CATG^[CT] PpuMI 37 GGGACCC [GA]G^G[AT]CC[CT] PstI 683 CTGCAG CTGCA^G SfcI 683 CTGCAG C^T[GA][CT]AG Tsp45I 158 GTGAC ^GT[GC]AC XcmI 10 CCACATCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 544 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AlwNI 400 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 481 CAGACTCTG BpmI 62 CTCCAG CTGGAG(16/14) 454 CTGGAG BseRI 715 GAGGAG GAGGAG(10/8) 718 GAGGAG BsmAI 205 GTCTC GTCTC(1/5) 396 GTCTC BsmFI 37 GGGAC GGGAC(10/14) 338 GGGAC BsrI 95 ACTGG ACTGG(1/-1) 530 ACTGG HinfI 483 GACTC G^A[ACGT]TC 516 GACTC PleI 483 GACTC GAGTC(4/5) 516 GACTC SfaNI 450 GATGC GCATC(5/9) 572 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AluI AlwI BanII BbvI BcnI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PvuII Sau3AI Sau96I ScrFI TseI TspRI