Restriction map for
pFN21AB7983
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AlwI AlwNI AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 883 GTCTAC GT^[AC][GT]AC
AfaI 335 GTAC GT^AC
AgeI 912 ACCGGT A^CCGGT
Aor51HI 979 AGCGCT AGC^GCT
ApaI 107 GGGCCC GGGCC^C
BalI 1252 TGGCCA TGG^CCA
BanII 107 GGGCCC G[GA]GC[CT]^C
BbsI 824 GAAGAC GAAGAC(2/6)
BciVI 486 GTATCC GTATCC(6/5)
BglII 741 AGATCT A^GATCT
BpuAI 824 GAAGAC GAAGAC(2/6)
BseRI 1053 CTCCTC GAGGAG(10/8)
BsiEI 112 CGGCCG CG[GA][CT]^CG
BsrFI 912 ACCGGT [GA]^CCGG[CT]
BstYI 741 AGATCT [GA]^GATC[CT]
Cfr10I 912 ACCGGT [GA]^CCGG[CT]
DpnII 742 GATC ^GATC
DsaI 1007 CCATGG C^C[GA][CT]GG
EagI 112 CGGCCG C^GGCCG
EarI 797 GAAGAG CTCTTC(1/4)
EclXI 112 CGGCCG C^GGCCG
Eco47III 979 AGCGCT AGC^GCT
Eco52I 112 CGGCCG C^GGCCG
EcoRI 131 GAATTC G^AATTC
EcoT14I 1007 CCATGG C^C[AT][AT]GG
HaeII 979 AGCGCT [GA]GCGC^[CT]
HincII 46 GTCAAC GT[CT]^[GA]AC
HindII 46 GTCAAC GT[CT]^[GA]AC
Ksp632I 797 GAAGAG CTCTTC(1/4)
MaeIII 11 GTCAC ^GT[ACGT]AC
MboI 742 GATC ^GATC
MflI 741 AGATCT [GA]^GATC[CT]
MluNI 1252 TGGCCA TGG^CCA
MscI 1252 TGGCCA TGG^CCA
NcoI 1007 CCATGG C^CATGG
NdeI 1126 CATATG CA^TATG
NdeII 742 GATC ^GATC
PinAI 912 ACCGGT A^CCGGT
PstI 136 CTGCAG CTGCA^G
PvuII 613 CAGCTG CAG^CTG
RsaI 335 GTAC GT^AC
Sau3AI 742 GATC ^GATC
SphI 1067 GCATGC GCATG^C
StyI 1007 CCATGG C^C[AT][AT]GG
TaqI 780 TCGA T^CGA
Tsp45I 11 GTCAC ^GT[GC]AC
TthHB8I 780 TCGA T^CGA
XcmI 998 CCAGCTTCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 741 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 131 GAATTC [GA]^AATT[CT]
164 AAATTC
ApoI 131 GAATTC [GA]^AATT[CT]
164 AAATTC
AvaI 716 CCCGAG C^[CT]CG[GA]G
1164 CTCGGG
BpmI 239 CTCCAG CTGGAG(16/14)
673 CTGGAG
BsaWI 457 ACCGGA [AT]^CCGG[AT]
912 ACCGGT
BsiYI 458 CCGGAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
531 CCAGCAACAGG
BslI 458 CCGGAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
531 CCAGCAACAGG
BsmFI 493 GGGAC GGGAC(10/14)
1039 GTCCC
BsoBI 716 CCCGAG C^[CT]CG[GA]G
1164 CTCGGG
CfoI 413 GCGC GCG^C
980 GCGC
DdeI 436 CTAAG C^T[ACGT]AG
811 CTGAG
DraII 106 GGGGCCC [GA]G^G[ACGT]CC[CT]
668 AGGCCCT
EcoO109I 106 GGGGCCC [GA]G^G[ACGT]CC[CT]
668 AGGCCCT
HhaI 413 GCGC GCG^C
980 GCGC
HinP1I 413 GCGC G^CGC
980 GCGC
HphI 1089 GGTGA GGTGA(8/7)
1004 TCACC
NheI 93 GCTAGC G^CTAGC
391 GCTAGC
NspI 422 GCATGT [GA]CATG^[CT]
1067 GCATGC
SfcI 136 CTGCAG C^T[GA][CT]AG
975 CTACAG
TspRI 59 CACTG CA[GC]TG[ACGT][ACGT]^
879 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BanI BbvI BcnI
BfaI BmyI BsaJI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
TfiI TseI Tsp509I