Restriction map for
pFN21AB7983
(Linear) (Six-base) MAPSORT of: KIBB7983.seq Check: 2765 from: 1 to: 1266
KIBB7983.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 129 1266
Size: 129 1137
AccI GT'mk_AC
Cuts at: 0 884 1266
Size: 884 382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 637 770 1266
Size: 595 42 133 496
Fragments arranged by size:
595 496 133 42
AfeI AGC'GCT
Cuts at: 0 981 1266
Size: 981 285
AgeI A'CCGG_T
Cuts at: 0 912 1266
Size: 912 354
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 684 716 1266
Size: 684 32 550
Fragments arranged by size:
684 550 32
ApaI G_GGCC'C
Cuts at: 0 111 1266
Size: 111 1155
ApoI r'AATT_y
Cuts at: 0 131 164 1266
Size: 131 33 1102
Fragments arranged by size:
1102 131 33
AvaI C'yCGr_G
Cuts at: 0 716 1164 1266
Size: 716 448 102
Fragments arranged by size:
716 448 102
BanI G'GyrC_C
Cuts at: 0 469 501 1159 1168 1266
Size: 469 32 658 9 98
Fragments arranged by size:
658 469 98 32 9
BanII G_rGCy'C
Cuts at: 0 111 1266
Size: 111 1155
BbsI GAAGACnn'nnnn_
Cuts at: 0 831 1266
Size: 831 435
BciVI GTATCCnnnnn_n'
Cuts at: 0 497 1266
Size: 497 769
BglII A'GATC_T
Cuts at: 0 741 1266
Size: 741 525
Bme1580I G_kGCm'C
Cuts at: 0 111 283 472 1162 1171 1266
Size: 111 172 189 690 9 95
Fragments arranged by size:
690 189 172 111 95 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 258 1030 1266
Size: 258 772 236
Fragments arranged by size:
772 258 236
BmtI G_CTAG'C
Cuts at: 0 97 395 1266
Size: 97 298 871
Fragments arranged by size:
871 298 97
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 1266
Size: 207 1059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 694 1266
Size: 224 470 572
Fragments arranged by size:
572 470 224
BsaWI w'CCGG_w
Cuts at: 0 457 912 1266
Size: 457 455 354
Fragments arranged by size:
457 455 354
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 667 697 1266
Size: 268 30 369 30 569
Fragments arranged by size:
569 369 268 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1044 1266
Size: 1044 222
BseYI C'CCAG_C
Cuts at: 0 464 997 1042 1266
Size: 464 533 45 224
Fragments arranged by size:
533 464 224 45
BsiEI CG_ry'CG
Cuts at: 0 115 1266
Size: 115 1151
Bsp1286I G_dGCh'C
Cuts at: 0 111 283 472 1162 1171 1266
Size: 111 172 189 690 9 95
Fragments arranged by size:
690 189 172 111 95 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 164 1028 1266
Size: 129 35 864 238
Fragments arranged by size:
864 238 129 35
BsrFI r'CCGG_y
Cuts at: 0 912 1266
Size: 912 354
BstYI r'GATC_y
Cuts at: 0 741 1266
Size: 741 525
BtgI C'CryG_G
Cuts at: 0 1007 1266
Size: 1007 259
BtsI GCAGTG_nn'
Cuts at: 0 58 1266
Size: 58 1208
EaeI y'GGCC_r
Cuts at: 0 55 112 1252 1266
Size: 55 57 1140 14
Fragments arranged by size:
1140 57 55 14
EagI C'GGCC_G
Cuts at: 0 112 1266
Size: 112 1154
EarI CTCTTCn'nnn_
Cuts at: 0 792 1266
Size: 792 474
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1113 1266
Size: 1113 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 595 637 694 770 1266
Size: 224 371 42 57 76 496
Fragments arranged by size:
496 371 224 76 57 42
EcoO109I rG'GnC_Cy
Cuts at: 0 107 669 1266
Size: 107 562 597
Fragments arranged by size:
597 562 107
EcoRI G'AATT_C
Cuts at: 0 131 1266
Size: 131 1135
HaeII r_GCGC'y
Cuts at: 0 983 1266
Size: 983 283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 1266
Size: 268 32 966
Fragments arranged by size:
966 268 32
HincII GTy'rAC
Cuts at: 0 48 1266
Size: 48 1218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 599 1266
Size: 170 429 667
Fragments arranged by size:
667 429 170
MscI TGG'CCA
Cuts at: 0 1254 1266
Size: 1254 12
MspA1I CmG'CkG
Cuts at: 0 17 104 615 1266
Size: 17 87 511 651
Fragments arranged by size:
651 511 87 17
NcoI C'CATG_G
Cuts at: 0 1007 1266
Size: 1007 259
NdeI CA'TA_TG
Cuts at: 0 1127 1266
Size: 1127 139
NheI G'CTAG_C
Cuts at: 0 93 391 1266
Size: 93 298 875
Fragments arranged by size:
875 298 93
NspI r_CATG'y
Cuts at: 0 426 1071 1266
Size: 426 645 195
Fragments arranged by size:
645 426 195
PasI CC'CwG_GG
Cuts at: 0 203 1266
Size: 203 1063
PfoI T'CCnGG_A
Cuts at: 0 1221 1266
Size: 1221 45
PspOMI G'GGCC_C
Cuts at: 0 107 1266
Size: 107 1159
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 633 665 1266
Size: 633 32 601
Fragments arranged by size:
633 601 32
PstI C_TGCA'G
Cuts at: 0 140 1266
Size: 140 1126
PvuII CAG'CTG
Cuts at: 0 615 1266
Size: 615 651
SfcI C'TryA_G
Cuts at: 0 136 975 1266
Size: 136 839 291
Fragments arranged by size:
839 291 136
SphI G_CATG'C
Cuts at: 0 1071 1266
Size: 1071 195
StyI C'CwwG_G
Cuts at: 0 1007 1266
Size: 1007 259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1005 1266
Size: 1005 261
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI AloI ApaI ApoI
AvaI BanI BanII BbsI BciVI BglII Bme1580I BmrI
BmtI BplI BpmI BsaWI BsaXI BseRI BseYI BsiEI
Bsp1286I BspMI BsrFI BstYI BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII
MmeI MscI MspA1I NcoI NdeI NheI NspI PasI
PfoI PspOMI PsrI PstI PvuII SfcI SphI StyI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AhdI AleI AlfI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NgoMIV NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI