Restriction map for pFN21AE1347
 (Circular) (Six-base) MAPSORT of: pFN21AE1347.seq  Check: 1591  from: 1  to: 5842



pFN21AE1347  5842 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:43  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3652     278 
   Size:         53      83     186     936    2116    2468

  Fragments arranged by size: 

                2468    2116     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3185    1937 
   Size:       1248    4594
 
Acc65I G'GTAC_C

Cuts at:   3163    3163 
   Size:       5842
 
AclI AA'CG_TT

Cuts at:   1563    3969    4342    1563 
   Size:       2406     373    3063

  Fragments arranged by size: 

                3063    2406     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2234    3905    5289    5557    1632 
   Size:        602    1671    1384     268    1917

  Fragments arranged by size: 

                1917    1671    1384     602     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5645
 
AflIII A'CryG_T

Cuts at:   1203    4741    5718    1203 
   Size:       3538     977    1327

  Fragments arranged by size: 

                3538    1327     977
 
AgeI A'CCGG_T

Cuts at:   4673    4673 
   Size:       5842
 
AhdI GACnn_n'nnGTC

Cuts at:   4571    4571 
   Size:       5842
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5842
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5842
 
AlwNI CAG_nnn'CTG

Cuts at:   2074    2406    3585    5157    2074 
   Size:        332    1179    1572    2759

  Fragments arranged by size: 

                2759    1572    1179     332
 
ApaLI G'TGCA_C

Cuts at:   2445    2502    3899    5055    2445 
   Size:         57    1397    1156    3232

  Fragments arranged by size: 

                3232    1397    1156      57
 
ApoI r'AATT_y

Cuts at:   1493    2243    2329    3151    3377    3529    5638    1493 
   Size:        750      86     822     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     822     750     226     152      86
 
AseI AT'TA_AT

Cuts at:    160    1032    4396     160 
   Size:        872    3364    1606

  Fragments arranged by size: 

                3364    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5842
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2337    3167    1870 
   Size:         73     394     830    4545

  Fragments arranged by size: 

                4545     830     394      73
 
AvrII C'CTAG_G

Cuts at:   2762    2762 
   Size:       5842
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2544    2577    2544 
   Size:         33    5809
 
BamHI G'GATC_C

Cuts at:   3172    3539    3172 
   Size:        367    5475
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3163    4618     618 
   Size:        325     217     627    1376    1455    1842

  Fragments arranged by size: 

                1842    1455    1376     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2842    3161    1391 
   Size:       1451     319    4072

  Fragments arranged by size: 

                4072    1451     319
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3033     928 
   Size:        971    1134    3737

  Fragments arranged by size: 

                3737    1134     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4033    4067    1903 
   Size:         34    2096      34    3678

  Fragments arranged by size: 

                3678    2096      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3109    3734    4944    3109 
   Size:        625    1210    4007

  Fragments arranged by size: 

                4007    1210     625
 
BclI T'GATC_A

Cuts at:   1548    2397    2901    1548 
   Size:        849     504    4489

  Fragments arranged by size: 

                4489     849     504
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    4453     136 
   Size:        107     122      71     631     605     337    2444    1525

  Fragments arranged by size: 

                2444    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2890    3088    5837    2890 
   Size:        198    2749    2895

  Fragments arranged by size: 

                2895    2749     198
 
BlpI GC'TnA_GC

Cuts at:   3259    3259 
   Size:       5842
 
Bme1580I G_kGCm'C

Cuts at:   1790    2449    2506    3903    5059    1790 
   Size:        659      57    1397    1156    2573

  Fragments arranged by size: 

                2573    1397    1156     659      57
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5842
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2445    3573    4531 
   Size:        416      53      83     399      24    1028    1128     958

Cuts at:   4531     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1128    1028     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5842
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2096    2958    4502    1239 
   Size:        857     862    1544    2579

  Fragments arranged by size: 

                2579    1544     862     857
 
Bpu10I CC'TnA_GC

Cuts at:   2727    2727 
   Size:       5842
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3967    4832    5130    3967 
   Size:        865     298    4679

  Fragments arranged by size: 

                4679     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2318    4505     882 
   Size:        617     819    2187    2219

  Fragments arranged by size: 

                2219    2187     819     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3633     493 
   Size:        711    2429    2702

  Fragments arranged by size: 

                2702    2429     711
 
BsaBI GATnn'nnATC

Cuts at:   5836    5836 
   Size:       5842
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3649    4031    4666     275 
   Size:         53      83     186     936    2116     382     635    1451

  Fragments arranged by size: 

                2116    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4275    4673    4947    5094    5505    4275 
   Size:        398     274     147     411    4612

  Fragments arranged by size: 

                4612     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5617

  Fragments arranged by size: 

                5617     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2051    2156    1186 
   Size:        865     105    4872

  Fragments arranged by size: 

                4872     865     105
 
BseYI C'CCAG_C

Cuts at:   3102    5045    5541    3102 
   Size:       1943     496    3403

  Fragments arranged by size: 

                3403    1943     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2087    1163 
   Size:        573     351    4918

  Fragments arranged by size: 

                4918     573     351
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2022    2218    3292    4053    5081    1540 
   Size:        275     186      21     196    1074     761    1028    2301

  Fragments arranged by size: 

                2301    1074    1028     761     275     196     186      21
            
 
BsiHKAI G_wGCw'C

Cuts at:   2449    2506    3903    3988    5059    2449 
   Size:         57    1397      85    1071    3232

  Fragments arranged by size: 

                3232    1397    1071      85      57
 
BsmI GAATG_Cn'

Cuts at:   3353    3446    3353 
   Size:         93    5749
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2449    2506    2842    3161    3903    3988    5059 
   Size:        399     659      57     336     319     742      85    1071

Cuts at:   5059    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     659     399     336     319      85
                  57
 
BspEI T'CCGG_A

Cuts at:   5505    5505 
   Size:       5842
 
BspHI T'CATG_A

Cuts at:   3731    5461    3731 
   Size:       1730    4112
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2067    3197     844 
   Size:       1223    1130    3489

  Fragments arranged by size: 

                3489    1223    1130
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3729    1394 
   Size:        429    1906    3507

  Fragments arranged by size: 

                3507    1906     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4337    4511      65 
   Size:       1183    3089     174    1396

  Fragments arranged by size: 

                3089    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4486    4673    1700 
   Size:        110    2676     187    2869

  Fragments arranged by size: 

                2869    2676     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2464      96 
   Size:       2368    3474
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5842
 
BssSI C'ACGA_G

Cuts at:   3902    4914    3902 
   Size:       1012    4830
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5842
 
BstBI TT'CG_AA

Cuts at:   4646    4646 
   Size:       5842
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5842
 
BstYI r'GATC_y

Cuts at:   1972    2624    2890    3088    3172    3539    3924    3941    5393 
   Size:        652     266     198      84     367     385      17    1452

Cuts at:   5393    5479    5491    5837    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     652     385     367     346     266     198
                  86      84      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4351

  Fragments arranged by size: 

                4351     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2966     487 
   Size:        683      56    1740    3363

  Fragments arranged by size: 

                3363    1740     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2031    2303    3358    4152    4172    2031 
   Size:        272    1055     794      20    3701

  Fragments arranged by size: 

                3701    1055     794     272      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5842
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5807
 
DraI TTT'AAA

Cuts at:   2202    2850    3146    2202 
   Size:        648     296    4898

  Fragments arranged by size: 

                4898     648     296
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4849     809 
   Size:        507    3533    1802

  Fragments arranged by size: 

                3533    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2019    2031    3289 
   Size:         54    1425      50     275      18     189      12    1258

Cuts at:   3289    4178    5807       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425    1258     889     275     189      54      50
                  43      18      12
 
EagI C'GGCC_G

Cuts at:   1537    1812    2019    3289    1537 
   Size:        275     207    1270    4090

  Fragments arranged by size: 

                4090    1270     275     207
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3009    3772    1353 
   Size:       1656     763    3423

  Fragments arranged by size: 

                3423    1656     763
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4429    4803    4949     387 
   Size:       1064     138    2840     374     146    1280

  Fragments arranged by size: 

                2840    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2096    2234    2958    3905    4502    5289    5557 
   Size:        393     464     138     724     947     597     787     268

Cuts at:   5557    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     947     787     724     597     464     393     268
                 138
 
EcoO109I rG'GnC_Cy

Cuts at:   2056    2089    2056 
   Size:         33    5809
 
EcoRI G'AATT_C

Cuts at:   3151    3529    3151 
   Size:        378    5464
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2465    2687    3047    2465 
   Size:        222     360    5260

  Fragments arranged by size: 

                5260     360     222
 
FspI TGC'GCA

Cuts at:   4348    4348 
   Size:       5842
 
HaeII r_GCGC'y

Cuts at:   1678    2407    4989    1678 
   Size:        729    2582    2531

  Fragments arranged by size: 

                2582    2531     729
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4483    4515    4557    4589    1373 
   Size:         32     135      32    2911      32      42      32    2626

  Fragments arranged by size: 

                2911    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2411    3186    3432     669 
   Size:        988     281     473     775     246    3079

  Fragments arranged by size: 

                3079     988     775     473     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3068     748 
   Size:       2320    3522
 
HpaI GTT'AAC

Cuts at:   3432    3432 
   Size:       5842
 
KpnI G_GTAC'C

Cuts at:   3167    3167 
   Size:       5842
 
MfeI C'AATT_G

Cuts at:   3441    3441 
   Size:       5842
 
MluI A'CGCG_T

Cuts at:   5718    5718 
   Size:       5842
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4956    5140    1305 
   Size:       3651     184    2007

  Fragments arranged by size: 

                3651    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4020

  Fragments arranged by size: 

                4020    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3800    4159    4318     518 
   Size:        648    2634     359     159    2042

  Fragments arranged by size: 

                2634    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2085    2123    3258    3935    5083    5328    1606 
   Size:        479      38    1135     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1135     677     479     245      38
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4351

  Fragments arranged by size: 

                4351     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5842
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5842
 
NotI GC'GGCC_GC

Cuts at:   2019    3289    2019 
   Size:       1270    4572
 
NspI r_CATG'y

Cuts at:   1132    1229    3200    4745    1132 
   Size:         97    1971    1545    2229

  Fragments arranged by size: 

                2229    1971    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5842
 
PciI A'CATG_T

Cuts at:   4741    4741 
   Size:       5842
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2435    1108 
   Size:       1327    4515
 
PmeI GTTT'AAAC

Cuts at:   3146    3146 
   Size:       5842
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3911    3943    5424    5456    3911 
   Size:         32    1481      32    4297

  Fragments arranged by size: 

                4297    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2056    2089    2056 
   Size:         33    5809
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5842
 
PsiI TTA'TAA

Cuts at:   3412    3412 
   Size:       5842
 
PstI C_TGCA'G

Cuts at:    830    3194     830 
   Size:       2364    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5842
 
PvuII CAG'CTG

Cuts at:   2123    2123 
   Size:       5842
 
SalI G'TCGA_C

Cuts at:   1936    3184    1936 
   Size:       1248    4594
 
SbfI CC_TGCA'GG

Cuts at:   3194    3194 
   Size:       5842
 
ScaI AGT'ACT

Cuts at:   4090    5802    4090 
   Size:       1712    4130
 
SfcI C'TryA_G

Cuts at:    826    1045    3190    4325    5006    5197     826 
   Size:        219    2145    1135     681     191    1471

  Fragments arranged by size: 

                2145    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5842
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5842
 
SmaI CCC'GGG

Cuts at:   1872    3169    1872 
   Size:       1297    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3946    4847    5109     820 
   Size:        197     926    2003     901     262    1553

  Fragments arranged by size: 

                2003    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3633     493 
   Size:       3140    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5842
 
SphI G_CATG'C

Cuts at:   1132    3200    1132 
   Size:       2068    3774
 
SspI AAT'ATT

Cuts at:      5      52    3766       5 
   Size:         47    3714    2081

  Fragments arranged by size: 

                3714    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5842
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2762    3281     513 
   Size:        552     939     758     519    3074

  Fragments arranged by size: 

                3074     939     758     552     519
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2363    3869    3886    4039    4224    4669     325 
   Size:       1255     783    1506      17     153     185     445    1498

  Fragments arranged by size: 

                1506    1498    1255     783     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2464    4088    5800 
   Size:        274      80      33      51    1443     487    1624    1712

Cuts at:   5800      96 
   Size:        138

  Fragments arranged by size: 

                1712    1624    1443     487     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2588    2620    5802    5834    2588 
   Size:         32    3182      32    2596

  Fragments arranged by size: 

                3182    2596      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5842
 
XbaI T'CTAG_A

Cuts at:   3178    3178 
   Size:       5842
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5842
 
XmaI C'CCGG_G

Cuts at:   1870    3167    1870 
   Size:       1297    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2247    3971    2247 
   Size:       1724    4118
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3650     276 
   Size:         53      83     186     936    2116    2468

  Fragments arranged by size: 

                2468    2116     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI     BbeI    BbvCI    BfrBI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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