Restriction map for
pFN21AB6693
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
Acc65I AccI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HgaI HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 105 CGCG CG^CG
AccIII 182 TCCGGA T^CCGGA
AclI 625 AACGTT AA^CGTT
AlwNI 701 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 97 GGGCCC GGGCC^C
AseI 283 ATTAAT AT^TAAT
AsnI 283 ATTAAT AT^TAAT
AspHI 1109 GTGCTC G[AT]GC[AT]^C
AvrII 52 CCTAGG C^CTAGG
BanI 48 GGTGCC G^G[CT][GA]CC
BanII 97 GGGCCC G[GA]GC[CT]^C
BlnI 52 CCTAGG C^CTAGG
BsaI 841 GAGACC GGTCTC(1/5)
BsaWI 182 TCCGGA [AT]^CCGG[AT]
BseAI 182 TCCGGA T^CCGGA
BsgI 956 GTGCAG GTGCAG(16/14)
BsiHKAI 1109 GTGCTC G[AT]GC[AT]^C
BspEI 182 TCCGGA T^CCGGA
BsrBI 91 GAGCGG CCGCTC(-3/-3)
BsrI 505 CCAGT ACTGG(1/-1)
BstF5I 570 GGATG GGATG(2/0)
BstUI 105 CGCG CG^CG
Cac8I 1248 GCATGC GC[ACGT]^[ACGT]GC
CfoI 104 GCGC GCG^C
DraI 630 TTTAAA TTT^AAA
DsaI 60 CCGTGG C^C[GA][CT]GG
EaeI 14 TGGCCG [CT]^GGCC[GA]
EcoT22I 1246 ATGCAT ATGCA^T
FokI 570 GGATG GGATG(9/13)
HhaI 104 GCGC GCG^C
HinP1I 104 GCGC G^CGC
HincII 793 GTTGAC GT[CT]^[GA]AC
HindII 793 GTTGAC GT[CT]^[GA]AC
MroI 182 TCCGGA T^CCGGA
MspA1I 970 CAGCTG C[AC]G^C[GT]G
MvnI 105 CGCG CG^CG
NsiI 1246 ATGCAT ATGCA^T
PleI 1031 GACTC GAGTC(4/5)
PpuMI 55 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 283 ATTAAT AT^TAAT
Psp1406I 625 AACGTT AA^CGTT
PvuII 970 CAGCTG CAG^CTG
SapI 186 GAAGAGC GCTCTTC(1/4)
SphI 1248 GCATGC GCATG^C
TspRI 224 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 27 GACGTC GACGT^C
296 GACGTC
AcsI 577 GAATTT [GA]^AATT[CT]
936 GAATTT
AcyI 27 GACGTC G[GA]^CG[CT]C
296 GACGTC
ApoI 577 GAATTT [GA]^AATT[CT]
936 GAATTT
AvaII 56 GGTCC G^G[AT]CC
71 GGACC
BcnI 74 CCGGG CC^[GC]GG
100 CCCGG
BmyI 97 GGGCCC G[AGT]GC[ACT]^C
1109 GTGCTC
BsaHI 27 GACGTC G[GA]^CG[CT]C
296 GACGTC
Bsp1286I 97 GGGCCC G[AGT]GC[ACT]^C
1109 GTGCTC
BstNI 238 CCTGG CC^[AT]GG
485 CCTGG
DdeI 973 CTGAG C^T[ACGT]AG
1040 CTGAG
DpnII 619 GATC ^GATC
897 GATC
EarI 186 GAAGAG CTCTTC(1/4)
589 GAAGAG
EcoNI 485 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1035 CCTCTCTGAGG
EcoRII 238 CCTGG ^CC[AT]GG
485 CCTGG
EcoT14I 52 CCTAGG C^C[AT][AT]GG
845 CCAAGG
FauI 93 GCGGG CCCGC(4/6)
292 GCGGG
Hin1I 27 GACGTC G[GA]^CG[CT]C
296 GACGTC
Ksp632I 186 GAAGAG CTCTTC(1/4)
589 GAAGAG
MboI 619 GATC ^GATC
897 GATC
MslI 117 CATGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
811 CACAGAAGTG
MvaI 238 CCTGG CC^[AT]GG
485 CCTGG
NciI 74 CCGGG CC^[GC]GG
100 CCCGG
NdeII 619 GATC ^GATC
897 GATC
PstI 383 CTGCAG CTGCA^G
707 CTGCAG
Sau3AI 619 GATC ^GATC
897 GATC
SfaNI 544 GATGC GCATC(5/9)
703 GATGC
StyI 52 CCTAGG C^C[AT][AT]GG
845 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AflIII AluI BbvI
BfaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BspLU11I BssKI Cfr13I
DraII EcoO109I Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboII MnlI MseI
MspI MwoI NlaIII NlaIV NspI
RsaI Sau96I ScrFI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
XcmI