Restriction map for pFN21AB6693
 (Circular) (Six-base) MAPSORT of: pFN21AB6693.seq  Check: 315  from: 1  to: 5992



pFN21AB6693  5992 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:58  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2036    2305    3802     278 
   Size:         53      83     186     936     500     269    1497    2468

  Fragments arranged by size: 

                2468    1497     936     500     269     186      83      53
            
 
AccI GT'mk_AC

Cuts at:   1937    3335    1937 
   Size:       1398    4594
 
Acc65I G'GTAC_C

Cuts at:   3313    3313 
   Size:       5992
 
AclI AA'CG_TT

Cuts at:   1563    2631    4119    4492    1563 
   Size:       1068    1488     373    3063

  Fragments arranged by size: 

                3063    1488    1068     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2177    2222    2481    3173    3242    4055    5439    5707 
   Size:        545      45     259     692      69     813    1384     268

Cuts at:   5707    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1384     813     692     545     268     259      69
                  45
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5795
 
AflIII A'CryG_T

Cuts at:   1203    2121    2259    2431    2737    4891    5868    1203 
   Size:        918     138     172     306    2154     977    1327

  Fragments arranged by size: 

                2154    1327     977     918     306     172     138
 
AgeI A'CCGG_T

Cuts at:   4823    4823 
   Size:       5992
 
AhdI GACnn_n'nnGTC

Cuts at:   4721    4721 
   Size:       5992
 
AleI CACnn'nnGTG

Cuts at:   1166    2820    1166 
   Size:       1654    4338
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5992
 
AlwNI CAG_nnn'CTG

Cuts at:   2711    3735    5307    2711 
   Size:       1024    1572    3396

  Fragments arranged by size: 

                3396    1572    1024
 
ApaI G_GGCC'C

Cuts at:   2106    2106 
   Size:       5992
 
ApaLI G'TGCA_C

Cuts at:   4049    5205    4049 
   Size:       1156    4836
 
ApoI r'AATT_y

Cuts at:   1493    2582    2941    3301    3527    3679    5788    1493 
   Size:       1089     359     360     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1089     360     359     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2289    4546     160 
   Size:        872    1257    2257    1606

  Fragments arranged by size: 

                2257    1606    1257     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5992
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3317    1870 
   Size:         73    1374    4545

  Fragments arranged by size: 

                4545    1374      73
 
AvrII C'CTAG_G

Cuts at:   2057    2057 
   Size:       5992
 
BamHI G'GATC_C

Cuts at:   3322    3689    3322 
   Size:        367    5625
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2053    3313    4768     618 
   Size:        325     217     627     266    1260    1455    1842

  Fragments arranged by size: 

                1842    1455    1260     627     325     266     217
 
BanII G_rGCy'C

Cuts at:   1391    2106    3311    1391 
   Size:        715    1205    4072

  Fragments arranged by size: 

                4072    1205     715
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5021
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4183    4217    1903 
   Size:         34    2246      34    3678

  Fragments arranged by size: 

                3678    2246      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3884    5094    3884 
   Size:       1210    4782
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5992
 
BfrBI ATG'CAT

Cuts at:   3253    3253 
   Size:       5992
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4603     136 
   Size:        107     122      71     631     605    2931    1525

  Fragments arranged by size: 

                2931    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5987    5987 
   Size:       5992
 
BlpI GC'TnA_GC

Cuts at:   3409    3409 
   Size:       5992
 
Bme1580I G_kGCm'C

Cuts at:   1790    2106    4053    5209    1790 
   Size:        316    1947    1156    2573

  Fragments arranged by size: 

                2573    1947    1156     316
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5992
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2504    3723    4681 
   Size:        416      53      83     399      24    1087    1219     958

Cuts at:   4681     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1219    1087     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5992
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4652    1239 
   Size:       3413    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2304    2952    2957    4117    4982    5280    2304 
   Size:        648       5    1160     865     298    3016

  Fragments arranged by size: 

                3016    1160     865     648     298       5
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2840    4655     882 
   Size:        617    1341    1815    2219

  Fragments arranged by size: 

                2219    1815    1341     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3783     493 
   Size:        711    2579    2702

  Fragments arranged by size: 

                2702    2579     711
 
BsaBI GATnn'nnATC

Cuts at:   5986    5986 
   Size:       5992
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2033    2302    3799    4181 
   Size:         53      83     186     936     500     269    1497     382

Cuts at:   4181    4816     275 
   Size:        635    1451

  Fragments arranged by size: 

                1497    1451     936     635     500     382     269     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   2187    4425    4823    5097    5244    5655    2187 
   Size:       2238     398     274     147     411    2524

  Fragments arranged by size: 

                2524    2238     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2418    2448    1178 
   Size:         30     165      30    1015      30    4722

  Fragments arranged by size: 

                4722    1015     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2189    2192    3029    3030    1186 
   Size:       1003       3     837       1    4148

  Fragments arranged by size: 

                4148    1003     837       3       1
 
BseYI C'CCAG_C

Cuts at:   2973    5195    5691    2973 
   Size:       2222     496    3274

  Fragments arranged by size: 

                3274    2222     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2982    1163 
   Size:        573    1246    4173

  Fragments arranged by size: 

                4173    1246     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3442    4203    5231    1540 
   Size:        275     186    1441     761    1028    2301

  Fragments arranged by size: 

                2301    1441    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3118    4053    4138    5209    3118 
   Size:        935      85    1071    3901

  Fragments arranged by size: 

                3901    1071     935      85
 
BsmI GAATG_Cn'

Cuts at:   3503    3596    3503 
   Size:         93    5899
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2106    3118    3311    4053    4138    5209    1391 
   Size:        399     316    1012     193     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071    1012     742     399     316     193      85
            
 
BspEI T'CCGG_A

Cuts at:   2187    5655    2187 
   Size:       3468    2524
 
BspHI T'CATG_A

Cuts at:   3881    5611    3881 
   Size:       1730    4262
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3347     844 
   Size:       2503    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2098    3879    1394 
   Size:        429     275    1781    3507

  Fragments arranged by size: 

                3507    1781     429     275
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4487    4661      65 
   Size:       1183    3239     174    1396

  Fragments arranged by size: 

                3239    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4636    4823    1700 
   Size:        110    2826     187    2869

  Fragments arranged by size: 

                2869    2826     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5992
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5992
 
BssSI C'ACGA_G

Cuts at:   4052    5064    4052 
   Size:       1012    4980
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5992
 
BstBI TT'CG_AA

Cuts at:   4796    4796 
   Size:       5992
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5992
 
BstYI r'GATC_y

Cuts at:   1972    3322    3689    4074    4091    5543    5629    5641    5987 
   Size:       1350     367     385      17    1452      86      12     346

Cuts at:   5987    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1350     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2065     513 
   Size:        552    1000    4440

  Fragments arranged by size: 

                4440    1000     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5253

  Fragments arranged by size: 

                5253     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2228    3508    4302    4322    2228 
   Size:       1280     794      20    3898

  Fragments arranged by size: 

                3898    1280     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5992
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5957
 
DraI TTT'AAA

Cuts at:   2637    3296    2637 
   Size:        659    5333
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4999     809 
   Size:        507    3683    1802

  Fragments arranged by size: 

                3683    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2019    3439    4328 
   Size:         54    1425      50     275      18     189    1420     889

Cuts at:   4328    5957       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1420     889     275     189      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3439    1537 
   Size:        275    1627    4090

  Fragments arranged by size: 

                4090    1627     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2186    2589    3922    1353 
   Size:        833     403    1333    3423

  Fragments arranged by size: 

                3423    1333     833     403
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4579    4953    5099     387 
   Size:       1064     138    2990     374     146    1280

  Fragments arranged by size: 

                2990    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2177    2222    2481    3173    3242    4055    4652 
   Size:        393     545      45     259     692      69     813     597

Cuts at:   4652    5439    5707    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     813     787     692     597     545     393     268
                 259      69      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2494    3044    2494 
   Size:        550    5442
 
EcoO109I rG'GnC_Cy

Cuts at:   2061    2102    2206    2061 
   Size:         41     104    5847

  Fragments arranged by size: 

                5847     104      41
 
EcoRI G'AATT_C

Cuts at:   3301    3679    3301 
   Size:        378    5614
 
FspI TGC'GCA

Cuts at:   4498    4498 
   Size:       5992
 
HaeII r_GCGC'y

Cuts at:   1678    5139    1678 
   Size:       3461    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    1999    2031    2357    2389    4633 
   Size:         32     135      32     427      32     326      32    2244

Cuts at:   4633    4665    4707    4739    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2244     427     326     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2800    3336    3582     669 
   Size:        988     281     862     536     246    3079

  Fragments arranged by size: 

                3079     988     862     536     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5992
 
HpaI GTT'AAC

Cuts at:   3582    3582 
   Size:       5992
 
KpnI G_GTAC'C

Cuts at:   3317    3317 
   Size:       5992
 
MfeI C'AATT_G

Cuts at:   3591    3591 
   Size:       5992
 
MluI A'CGCG_T

Cuts at:   5868    5868 
   Size:       5992
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3258    5106    5290    1305 
   Size:       1953    1848     184    2007

  Fragments arranged by size: 

                2007    1953    1848     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4170

  Fragments arranged by size: 

                4170    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2126    2820    3950    4309    4468     518 
   Size:        648     960     694    1130     359     159    2042

  Fragments arranged by size: 

                2042    1130     960     694     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2977    3408    4085    5233    5478    1606 
   Size:       1371     431     677    1148     245    2120

  Fragments arranged by size: 

                2120    1371    1148     677     431     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5440
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5992
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5992
 
NotI GC'GGCC_GC

Cuts at:   3439    3439 
   Size:       5992
 
NsiI A_TGCA'T

Cuts at:   3255    3255 
   Size:       5992
 
NspI r_CATG'y

Cuts at:   1132    1229    2125    2263    2435    3253    3257    3350    4895 
   Size:         97     896     138     172     818       4      93    1545

Cuts at:   4895    1132 
   Size:       2229

  Fragments arranged by size: 

                2229    1545     896     818     172     138      97      93
                   4
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5992
 
PciI A'CATG_T

Cuts at:   2121    2259    2431    4891    2121 
   Size:        138     172    2460    3222

  Fragments arranged by size: 

                3222    2460     172     138
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5992
 
PmeI GTTT'AAAC

Cuts at:   3296    3296 
   Size:       5992
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2165    2197    4061    4093    5574    5606    2165 
   Size:         32    1864      32    1481      32    2551

  Fragments arranged by size: 

                2551    1864    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2061    2061 
   Size:       5992
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5992
 
PsiI TTA'TAA

Cuts at:   3562    3562 
   Size:       5992
 
PspOMI G'GGCC_C

Cuts at:   2102    2102 
   Size:       5992
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2427    2459    2727    2759    3189    3221    2427 
   Size:         32     268      32     430      32    5198

  Fragments arranged by size: 

                5198     430     268      32      32      32
 
PstI C_TGCA'G

Cuts at:    830    2392    2716    3344     830 
   Size:       1562     324     628    3478

  Fragments arranged by size: 

                3478    1562     628     324
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5992
 
PvuII CAG'CTG

Cuts at:   2977    2977 
   Size:       5992
 
SalI G'TCGA_C

Cuts at:   1936    3334    1936 
   Size:       1398    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2186    2186 
   Size:       5992
 
SbfI CC_TGCA'GG

Cuts at:   3344    3344 
   Size:       5992
 
ScaI AGT'ACT

Cuts at:   4240    5952    4240 
   Size:       1712    4280
 
SfcI C'TryA_G

Cuts at:    826    1045    2388    2712    3165    3340    4475    5156    5347 
   Size:        219    1343     324     453     175    1135     681     191

Cuts at:   5347     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1343    1135     681     453     324     219     191
                 175
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5992
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5992
 
SmaI CCC'GGG

Cuts at:   1872    3319    1872 
   Size:       1447    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2319    2936    2967    4096    4997    5259 
   Size:        197     926     376     617      31    1129     901     262

Cuts at:   5259     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1129     926     901     617     376     262     197
                  31
 
SnaBI TAC'GTA

Cuts at:    493    3783     493 
   Size:       3290    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5992
 
SphI G_CATG'C

Cuts at:   1132    3257    3350    1132 
   Size:       2125      93    3774

  Fragments arranged by size: 

                3774    2125      93
 
SspI AAT'ATT

Cuts at:      5      52    3916       5 
   Size:         47    3864    2081

  Fragments arranged by size: 

                3864    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5992
 
StyI C'CwwG_G

Cuts at:    513    1065    2057    2850    3431     513 
   Size:        552     992     793     581    3074

  Fragments arranged by size: 

                3074     992     793     581     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2361    4019    4036    4189    4374    4819     325 
   Size:       1255     781    1658      17     153     185     445    1498

  Fragments arranged by size: 

                1658    1498    1255     781     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2267    2740    2919 
   Size:        274      80      33      51    1443     290     473     179

Cuts at:   2919    4238    5950      96 
   Size:       1319    1712     138

  Fragments arranged by size: 

                1712    1443    1319     473     290     274     179     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3250    3282    5952    5984    3250 
   Size:         32    2670      32    3258

  Fragments arranged by size: 

                3258    2670      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5992
 
XbaI T'CTAG_A

Cuts at:   3328    3328 
   Size:       5992
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2819    2857    3008    2819 
   Size:         38     151    5803

  Fragments arranged by size: 

                5803     151      38
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5992
 
XmaI C'CCGG_G

Cuts at:   1870    3317    1870 
   Size:       1447    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4121    4121 
   Size:       5992
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2034    2303    3800     276 
   Size:         53      83     186     936     500     269    1497    2468

  Fragments arranged by size: 

                2468    1497     936     500     269     186      83      53
            
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PstI     PvuI    PvuII     SalI     SapI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI     BaeI     BbeI    BbvCI     BplI
   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     PacI     PfoI     PmlI    PspXI    RsrII     SacI    SacII
    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI
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