(Circular) (Six-base) MAPSORT of: pFN21AB6693.seq Check: 315 from: 1 to: 5992 pFN21AB6693 5992 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2036 2305 3802 278 Size: 53 83 186 936 500 269 1497 2468 Fragments arranged by size: 2468 1497 936 500 269 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 2631 4119 4492 1563 Size: 1068 1488 373 3063 Fragments arranged by size: 3063 1488 1068 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 2222 2481 3173 3242 4055 5439 5707 Size: 545 45 259 692 69 813 1384 268 Cuts at: 5707 1632 Size: 1917 Fragments arranged by size: 1917 1384 813 692 545 268 259 69 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 2121 2259 2431 2737 4891 5868 1203 Size: 918 138 172 306 2154 977 1327 Fragments arranged by size: 2154 1327 977 918 306 172 138 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 2820 1166 Size: 1654 4338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AlwNI CAG_nnn'CTG Cuts at: 2711 3735 5307 2711 Size: 1024 1572 3396 Fragments arranged by size: 3396 1572 1024 ApaI G_GGCC'C Cuts at: 2106 2106 Size: 5992 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 2582 2941 3301 3527 3679 5788 1493 Size: 1089 359 360 226 152 2109 1697 Fragments arranged by size: 2109 1697 1089 360 359 226 152 AseI AT'TA_AT Cuts at: 160 1032 2289 4546 160 Size: 872 1257 2257 1606 Fragments arranged by size: 2257 1606 1257 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 3317 1870 Size: 73 1374 4545 Fragments arranged by size: 4545 1374 73 AvrII C'CTAG_G Cuts at: 2057 2057 Size: 5992 BamHI G'GATC_C Cuts at: 3322 3689 3322 Size: 367 5625 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 3313 4768 618 Size: 325 217 627 266 1260 1455 1842 Fragments arranged by size: 1842 1455 1260 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 2106 3311 1391 Size: 715 1205 4072 Fragments arranged by size: 4072 1205 715 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5021 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4183 4217 1903 Size: 34 2246 34 3678 Fragments arranged by size: 3678 2246 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BfrBI ATG'CAT Cuts at: 3253 3253 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4603 136 Size: 107 122 71 631 605 2931 1525 Fragments arranged by size: 2931 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 2106 4053 5209 1790 Size: 316 1947 1156 2573 Fragments arranged by size: 2573 1947 1156 316 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2504 3723 4681 Size: 416 53 83 399 24 1087 1219 958 Cuts at: 4681 442 Size: 1753 Fragments arranged by size: 1753 1219 1087 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4652 1239 Size: 3413 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2304 2952 2957 4117 4982 5280 2304 Size: 648 5 1160 865 298 3016 Fragments arranged by size: 3016 1160 865 648 298 5 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2840 4655 882 Size: 617 1341 1815 2219 Fragments arranged by size: 2219 1815 1341 617 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 2302 3799 4181 Size: 53 83 186 936 500 269 1497 382 Cuts at: 4181 4816 275 Size: 635 1451 Fragments arranged by size: 1497 1451 936 635 500 382 269 186 83 53 BsaWI w'CCGG_w Cuts at: 2187 4425 4823 5097 5244 5655 2187 Size: 2238 398 274 147 411 2524 Fragments arranged by size: 2524 2238 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2418 2448 1178 Size: 30 165 30 1015 30 4722 Fragments arranged by size: 4722 1015 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 2192 3029 3030 1186 Size: 1003 3 837 1 4148 Fragments arranged by size: 4148 1003 837 3 1 BseYI C'CCAG_C Cuts at: 2973 5195 5691 2973 Size: 2222 496 3274 Fragments arranged by size: 3274 2222 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2982 1163 Size: 573 1246 4173 Fragments arranged by size: 4173 1246 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 4203 5231 1540 Size: 275 186 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3118 4053 4138 5209 3118 Size: 935 85 1071 3901 Fragments arranged by size: 3901 1071 935 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 3118 3311 4053 4138 5209 1391 Size: 399 316 1012 193 742 85 1071 2174 Fragments arranged by size: 2174 1071 1012 742 399 316 193 85 BspEI T'CCGG_A Cuts at: 2187 5655 2187 Size: 3468 2524 BspHI T'CATG_A Cuts at: 3881 5611 3881 Size: 1730 4262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3347 844 Size: 2503 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2098 3879 1394 Size: 429 275 1781 3507 Fragments arranged by size: 3507 1781 429 275 BsrDI GCAATG_nn' Cuts at: 65 1248 4487 4661 65 Size: 1183 3239 174 1396 Fragments arranged by size: 3239 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4636 4823 1700 Size: 110 2826 187 2869 Fragments arranged by size: 2869 2826 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 4052 5064 4052 Size: 1012 4980 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5992 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstYI r'GATC_y Cuts at: 1972 3322 3689 4074 4091 5543 5629 5641 5987 Size: 1350 367 385 17 1452 86 12 346 Cuts at: 5987 1972 Size: 1977 Fragments arranged by size: 1977 1452 1350 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2065 513 Size: 552 1000 4440 Fragments arranged by size: 4440 1000 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5253 Fragments arranged by size: 5253 683 56 BtsI GCAGTG_nn' Cuts at: 2228 3508 4302 4322 2228 Size: 1280 794 20 3898 Fragments arranged by size: 3898 1280 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 2637 3296 2637 Size: 659 5333 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 3439 4328 Size: 54 1425 50 275 18 189 1420 889 Cuts at: 4328 5957 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1420 889 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2589 3922 1353 Size: 833 403 1333 3423 Fragments arranged by size: 3423 1333 833 403 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4579 4953 5099 387 Size: 1064 138 2990 374 146 1280 Fragments arranged by size: 2990 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2222 2481 3173 3242 4055 4652 Size: 393 545 45 259 692 69 813 597 Cuts at: 4652 5439 5707 1239 Size: 787 268 1524 Fragments arranged by size: 1524 813 787 692 597 545 393 268 259 69 45 EcoNI CCTnn'n_nnAGG Cuts at: 2494 3044 2494 Size: 550 5442 EcoO109I rG'GnC_Cy Cuts at: 2061 2102 2206 2061 Size: 41 104 5847 Fragments arranged by size: 5847 104 41 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 5139 1678 Size: 3461 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2357 2389 4633 Size: 32 135 32 427 32 326 32 2244 Cuts at: 4633 4665 4707 4739 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2244 427 326 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2800 3336 3582 669 Size: 988 281 862 536 246 3079 Fragments arranged by size: 3079 988 862 536 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3258 5106 5290 1305 Size: 1953 1848 184 2007 Fragments arranged by size: 2007 1953 1848 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4170 Fragments arranged by size: 4170 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2820 3950 4309 4468 518 Size: 648 960 694 1130 359 159 2042 Fragments arranged by size: 2042 1130 960 694 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2977 3408 4085 5233 5478 1606 Size: 1371 431 677 1148 245 2120 Fragments arranged by size: 2120 1371 1148 677 431 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5440 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NsiI A_TGCA'T Cuts at: 3255 3255 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2125 2263 2435 3253 3257 3350 4895 Size: 97 896 138 172 818 4 93 1545 Cuts at: 4895 1132 Size: 2229 Fragments arranged by size: 2229 1545 896 818 172 138 97 93 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5992 PciI A'CATG_T Cuts at: 2121 2259 2431 4891 2121 Size: 138 172 2460 3222 Fragments arranged by size: 3222 2460 172 138 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2165 2197 4061 4093 5574 5606 2165 Size: 32 1864 32 1481 32 2551 Fragments arranged by size: 2551 1864 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2061 2061 Size: 5992 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PspOMI G'GGCC_C Cuts at: 2102 2102 Size: 5992 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2427 2459 2727 2759 3189 3221 2427 Size: 32 268 32 430 32 5198 Fragments arranged by size: 5198 430 268 32 32 32 PstI C_TGCA'G Cuts at: 830 2392 2716 3344 830 Size: 1562 324 628 3478 Fragments arranged by size: 3478 1562 628 324 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 2977 2977 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SapI GCTCTTCn'nnn_ Cuts at: 2186 2186 Size: 5992 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SfcI C'TryA_G Cuts at: 826 1045 2388 2712 3165 3340 4475 5156 5347 Size: 219 1343 324 453 175 1135 681 191 Cuts at: 5347 826 Size: 1471 Fragments arranged by size: 1471 1343 1135 681 453 324 219 191 175 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2319 2936 2967 4096 4997 5259 Size: 197 926 376 617 31 1129 901 262 Cuts at: 5259 820 Size: 1553 Fragments arranged by size: 1553 1129 926 901 617 376 262 197 31 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3257 3350 1132 Size: 2125 93 3774 Fragments arranged by size: 3774 2125 93 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5992 StyI C'CwwG_G Cuts at: 513 1065 2057 2850 3431 513 Size: 552 992 793 581 3074 Fragments arranged by size: 3074 992 793 581 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2361 4019 4036 4189 4374 4819 325 Size: 1255 781 1658 17 153 185 445 1498 Fragments arranged by size: 1658 1498 1255 781 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2267 2740 2919 Size: 274 80 33 51 1443 290 473 179 Cuts at: 2919 4238 5950 96 Size: 1319 1712 138 Fragments arranged by size: 1712 1443 1319 473 290 274 179 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3250 3282 5952 5984 3250 Size: 32 2670 32 3258 Fragments arranged by size: 3258 2670 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2819 2857 3008 2819 Size: 38 151 5803 Fragments arranged by size: 5803 151 38 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 4121 4121 Size: 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2034 2303 3800 276 Size: 53 83 186 936 500 269 1497 2468 Fragments arranged by size: 2468 1497 936 500 269 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI