(Circular) (Six-base) MAPSORT of: pFN21AE2507.seq Check: 5339 from: 1 to: 6025 pFN21AE2507 6025 bp With 182 enzymes: SgfI * February 14, 2014 11:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3368 1937 Size: 1431 4594 Acc65I G'GTAC_C Cuts at: 3144 3346 3144 Size: 202 5823 AclI AA'CG_TT Cuts at: 1563 4152 4525 1563 Size: 2589 373 3063 Fragments arranged by size: 3063 2589 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 2861 2878 2936 3065 4088 5472 5740 Size: 527 702 17 58 129 1023 1384 268 Cuts at: 5740 1632 Size: 1917 Fragments arranged by size: 1917 1384 1023 702 527 268 129 58 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 4924 5901 1203 Size: 3721 977 1327 Fragments arranged by size: 3721 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 2173 2326 4754 2173 Size: 153 2428 3444 Fragments arranged by size: 3444 2428 153 AleI CACnn'nnGTG Cuts at: 1166 3312 1166 Size: 2146 3879 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6025 AlwNI CAG_nnn'CTG Cuts at: 2452 2680 2697 2839 3191 3768 5340 2452 Size: 228 17 142 352 577 1572 3137 Fragments arranged by size: 3137 1572 577 352 228 142 17 ApaI G_GGCC'C Cuts at: 2073 2224 2912 2073 Size: 151 688 5186 Fragments arranged by size: 5186 688 151 ApaLI G'TGCA_C Cuts at: 2876 3113 4082 5238 2876 Size: 237 969 1156 3663 Fragments arranged by size: 3663 1156 969 237 ApoI r'AATT_y Cuts at: 1493 3334 3560 3712 5821 1493 Size: 1841 226 152 2109 1697 Fragments arranged by size: 2109 1841 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2799 3290 3350 1870 Size: 73 856 491 60 4545 Fragments arranged by size: 4545 856 491 73 60 BamHI G'GATC_C Cuts at: 2517 2853 3030 3355 3722 2517 Size: 336 177 325 367 4820 Fragments arranged by size: 4820 367 336 325 177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3144 3346 4801 618 Size: 325 217 627 1357 202 1455 1842 Fragments arranged by size: 1842 1455 1357 627 325 217 202 BanII G_rGCy'C Cuts at: 1391 2073 2224 2912 3126 3344 1391 Size: 682 151 688 214 218 4072 Fragments arranged by size: 4072 688 682 218 214 151 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5054 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4216 4250 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2156 4636 136 Size: 107 122 71 631 605 484 2480 1525 Fragments arranged by size: 2480 1525 631 605 484 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 3442 3442 Size: 6025 Bme1580I G_kGCm'C Cuts at: 1790 2073 2224 2367 2465 2880 2912 3117 4086 Size: 283 151 143 98 415 32 205 969 Cuts at: 4086 5242 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 969 415 283 205 151 143 98 32 BmgBI CAC'GTC Cuts at: 1318 2146 1318 Size: 828 5197 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3097 3756 4714 Size: 416 53 83 399 24 1680 659 958 Cuts at: 4714 442 Size: 1753 Fragments arranged by size: 1753 1680 958 659 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 4685 1239 Size: 1043 2403 2579 Fragments arranged by size: 2579 2403 1043 Bpu10I CC'TnA_GC Cuts at: 3120 3120 Size: 6025 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4150 5015 5313 4150 Size: 865 298 4862 Fragments arranged by size: 4862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2522 3297 4688 882 Size: 617 1023 775 1391 2219 Fragments arranged by size: 2219 1391 1023 775 617 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 2132 6019 2132 Size: 3887 2138 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2372 2501 3832 Size: 53 83 186 936 513 326 129 1331 Cuts at: 3832 4214 4849 275 Size: 382 635 1451 Fragments arranged by size: 1451 1331 936 635 513 382 326 186 129 83 53 BsaWI w'CCGG_w Cuts at: 2292 4458 4856 5130 5277 5688 2292 Size: 2166 398 274 147 411 2629 Fragments arranged by size: 2629 2166 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5800 Fragments arranged by size: 5800 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 3005 1186 Size: 1210 609 4206 Fragments arranged by size: 4206 1210 609 BseYI C'CCAG_C Cuts at: 2647 3184 3209 5228 5724 2647 Size: 537 25 2019 496 2948 Fragments arranged by size: 2948 2019 537 496 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 2101 2426 2693 2923 1163 Size: 573 324 41 325 267 230 4265 Fragments arranged by size: 4265 573 325 324 267 230 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2334 2391 3475 4236 5264 1540 Size: 275 186 333 57 1084 761 1028 2301 Fragments arranged by size: 2301 1084 1028 761 333 275 186 57 BsiHKAI G_wGCw'C Cuts at: 2607 2730 2880 3117 3126 4086 4171 5242 2607 Size: 123 150 237 9 960 85 1071 3390 Fragments arranged by size: 3390 1071 960 237 150 123 85 9 BsmI GAATG_Cn' Cuts at: 3536 3629 3536 Size: 93 5932 BsmBI CGTCTCn'nnnn_ Cuts at: 2378 2378 Size: 6025 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2224 2367 2465 2607 2730 2880 Size: 399 283 151 143 98 142 123 150 Cuts at: 2880 2912 3117 3126 3344 4086 4171 5242 1391 Size: 32 205 9 218 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 218 205 151 150 143 142 123 98 85 32 9 BspEI T'CCGG_A Cuts at: 2292 5688 2292 Size: 3396 2629 BspHI T'CATG_A Cuts at: 2061 3914 5644 2061 Size: 1853 1730 2442 Fragments arranged by size: 2442 1853 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2688 3380 844 Size: 1844 692 3489 Fragments arranged by size: 3489 1844 692 BsrBI CCG'CTC Cuts at: 1394 1823 2641 3912 1394 Size: 429 818 1271 3507 Fragments arranged by size: 3507 1271 818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2096 2346 4520 4694 65 Size: 1183 848 250 2174 174 1396 Fragments arranged by size: 2174 1396 1183 848 250 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2424 4669 4856 1700 Size: 110 614 2245 187 2869 Fragments arranged by size: 2869 2245 614 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6025 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 2466 2879 4085 5097 2466 Size: 413 1206 1012 3394 Fragments arranged by size: 3394 1206 1012 413 BstAPI GCAn_nnn'nTGC Cuts at: 794 2680 794 Size: 1886 4139 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6025 BstYI r'GATC_y Cuts at: 1972 2187 2487 2517 2853 3006 3030 3355 3722 Size: 215 300 30 336 153 24 325 367 Cuts at: 3722 4107 4124 5576 5662 5674 6020 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 336 325 300 215 153 86 30 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2081 2367 2521 2857 3229 513 Size: 552 939 77 286 154 336 372 3309 Fragments arranged by size: 3309 939 552 372 336 286 154 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 2308 2574 2710 3541 4335 4355 2308 Size: 266 136 831 794 20 3978 Fragments arranged by size: 3978 831 794 266 136 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5990 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2756 3107 5032 809 Size: 507 1440 351 1925 1802 Fragments arranged by size: 1925 1802 1440 507 351 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2148 2331 Size: 54 1425 50 275 18 277 41 183 Cuts at: 2331 2388 3274 3472 4361 5990 8 Size: 57 886 198 889 1629 43 Fragments arranged by size: 1629 1425 889 886 277 275 198 183 57 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2331 2388 3472 1537 Size: 275 519 57 1084 4090 Fragments arranged by size: 4090 1084 519 275 57 EarI CTCTTCn'nnn_ Cuts at: 1353 2612 2690 3955 1353 Size: 1259 78 1265 3423 Fragments arranged by size: 3423 1265 1259 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3311 4612 4986 5132 387 Size: 1064 138 1722 1301 374 146 1280 Fragments arranged by size: 1722 1301 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3124 3124 Size: 6025 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2282 2861 2878 2936 3065 4088 Size: 393 527 123 579 17 58 129 1023 Cuts at: 4088 4685 5472 5740 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1023 787 597 579 527 393 268 129 123 58 17 EcoNI CCTnn'n_nnAGG Cuts at: 2207 2843 2207 Size: 636 5389 EcoO109I rG'GnC_Cy Cuts at: 2032 2070 2200 2220 2221 2257 2677 2778 2836 Size: 38 130 20 1 36 420 101 58 Cuts at: 2836 2908 2909 2032 Size: 72 1 5148 Fragments arranged by size: 5148 420 130 101 72 58 38 36 20 1 1 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 2453 2511 2576 2648 2828 3216 5172 1678 Size: 775 58 65 72 180 388 1956 2531 Fragments arranged by size: 2531 1956 775 388 180 72 65 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2043 2075 2159 2191 2312 Size: 32 135 32 471 32 84 32 121 Cuts at: 2312 2344 2486 2518 2957 2989 4666 4698 4740 Size: 32 142 32 439 32 1677 32 42 Cuts at: 4740 4772 1373 Size: 32 2626 Fragments arranged by size: 2626 1677 471 439 142 135 121 84 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3058 3369 3615 669 Size: 988 281 1120 311 246 3079 Fragments arranged by size: 3079 1120 988 311 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6025 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KpnI G_GTAC'C Cuts at: 3148 3350 3148 Size: 202 5823 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2074 2607 2865 5139 5323 1305 Size: 769 533 258 2274 184 2007 Fragments arranged by size: 2274 2007 769 533 258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2150 3276 10 Size: 54 1425 343 318 1126 2759 Fragments arranged by size: 2759 1425 1126 343 318 54 MslI CAynn'nnrTG Cuts at: 518 1166 2981 3312 3983 4342 4501 518 Size: 648 1815 331 671 359 159 2042 Fragments arranged by size: 2042 1815 671 648 359 331 159 MspA1I CmG'CkG Cuts at: 1606 2083 2369 2523 2859 3188 3441 4118 5266 Size: 477 286 154 336 329 253 677 1148 Cuts at: 5266 5511 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 477 336 329 286 253 245 154 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 3383 4928 1132 Size: 97 2154 1545 2229 Fragments arranged by size: 2229 2154 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6025 PciI A'CATG_T Cuts at: 4924 4924 Size: 6025 PflMI CCAn_nnn'nTGG Cuts at: 1108 3087 1108 Size: 1979 4046 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2257 2677 2200 Size: 57 420 5548 Fragments arranged by size: 5548 420 57 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 3595 3595 Size: 6025 PspOMI G'GGCC_C Cuts at: 2069 2220 2908 2069 Size: 151 688 5186 Fragments arranged by size: 5186 688 151 PstI C_TGCA'G Cuts at: 830 2658 2676 3377 830 Size: 1828 18 701 3478 Fragments arranged by size: 3478 1828 701 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 3188 3188 Size: 6025 SacI G_AGCT'C Cuts at: 3126 3126 Size: 6025 SacII CC_GC'GG Cuts at: 2084 2370 2524 2860 2084 Size: 286 154 336 5249 Fragments arranged by size: 5249 336 286 154 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SapI GCTCTTCn'nnn_ Cuts at: 2612 2690 2612 Size: 78 5947 SbfI CC_TGCA'GG Cuts at: 2676 3377 2676 Size: 701 5324 ScaI AGT'ACT Cuts at: 4273 5985 4273 Size: 1712 4313 SfcI C'TryA_G Cuts at: 826 1045 2654 2672 3139 3373 4508 5189 5380 Size: 219 1609 18 467 234 1135 681 191 Cuts at: 5380 826 Size: 1471 Fragments arranged by size: 1609 1471 1135 681 467 234 219 191 18 SfiI GGCCn_nnn'nGGCC Cuts at: 2156 2156 Size: 6025 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 2801 3352 1872 Size: 929 551 4545 Fragments arranged by size: 4545 929 551 SmlI C'TyrA_G Cuts at: 820 1017 1943 4129 5030 5292 820 Size: 197 926 2186 901 262 1553 Fragments arranged by size: 2186 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 3383 1132 Size: 2251 3774 SspI AAT'ATT Cuts at: 5 52 3949 5 Size: 47 3897 2081 Fragments arranged by size: 3897 2081 47 StuI AGG'CCT Cuts at: 1517 2588 1517 Size: 1071 4954 StyI C'CwwG_G Cuts at: 513 1065 2004 2532 2583 3464 513 Size: 552 939 528 51 881 3074 Fragments arranged by size: 3074 939 881 552 528 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2941 3098 4052 4069 4222 4407 4852 Size: 1255 1361 157 954 17 153 185 445 Cuts at: 4852 325 Size: 1498 Fragments arranged by size: 1498 1361 1255 954 445 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4271 5983 96 Size: 274 80 33 51 1443 2294 1712 138 Fragments arranged by size: 2294 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2287 2319 2991 3023 5985 6017 2287 Size: 32 672 32 2962 32 2295 Fragments arranged by size: 2962 2295 672 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2550 1316 Size: 1234 4791 XbaI T'CTAG_A Cuts at: 3361 3361 Size: 6025 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 3319 2257 Size: 1062 4963 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6025 XmaI C'CCGG_G Cuts at: 1870 2799 3350 1870 Size: 929 551 4545 Fragments arranged by size: 4545 929 551 XmnI GAAnn'nnTTC Cuts at: 4154 4154 Size: 6025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI