################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiHKAI BsiWI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 712 CGCG CG^CG AgeI 977 ACCGGT A^CCGGT ApaI 367 GGGCCC GGGCC^C AspI 544 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 503 TGGCCA TGG^CCA BamHI 512 GGATCC G^GATCC BbvCI 214 GCTGAGG CCTCAGC(-5/-2) BsiEI 179 CGGCCG CG[GA][CT]^CG BsmBI 175 GAGACG CGTCTC(1/5) BsmI 287 GCATTC GAATGC(1/-1) BspDI 916 ATCGAT AT^CGAT BspMI 969 GCAGGT ACCTGC(4/8) BsrBI 109 GAGCGG CCGCTC(-3/-3) BstAPI 432 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 774 GGTGACC G^GT[ACGT]ACC BstPI 774 GGTGACC G^GT[ACGT]ACC BstUI 712 CGCG CG^CG BstXI 1160 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 512 GGATCC [GA]^GATC[CT] ClaI 916 ATCGAT AT^CGAT EagI 179 CGGCCG C^GGCCG EclXI 179 CGGCCG C^GGCCG Eco52I 179 CGGCCG C^GGCCG EcoO65I 774 GGTGACC G^GT[ACGT]ACC MaeII 908 ACGT A^CGT MflI 512 GGATCC [GA]^GATC[CT] MluNI 503 TGGCCA TGG^CCA MscI 503 TGGCCA TGG^CCA MspA1I 231 CAGCTG C[AC]G^C[GT]G MvnI 712 CGCG CG^CG NspI 1253 GCATGT [GA]CATG^[CT] PflFI 544 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 977 ACCGGT A^CCGGT PstI 748 CTGCAG CTGCA^G PvuII 231 CAGCTG CAG^CTG SexAI 1091 ACCAGGT A^CC[AT]GGT SfcI 748 CTGCAG C^T[GA][CT]AG SmlI 517 CTCAAG C^T[CT][GA]AG StuI 791 AGGCCT AGG^CCT TfiI 381 GATTC G^A[AT]TC Tth111I 544 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1088 CCAACCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 512 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 139 GGCGCC G[GA]^CG[CT]C 998 GGCGCC BanII 367 GGGCCC G[GA]GC[CT]^C 707 GAGCCC BbeI 139 GGCGCC GGCGC^C 998 GGCGCC BcnI 88 CCGGG CC^[GC]GG 585 CCGGG BsaHI 139 GGCGCC G[GA]^CG[CT]C 998 GGCGCC BsaI 193 GAGACC GGTCTC(1/5) 466 GAGACC BsaWI 291 TCCGGT [AT]^CCGG[AT] 977 ACCGGT BseRI 322 GAGGAG GAGGAG(10/8) 463 GAGGAG BsgI 160 CTGCAC GTGCAG(16/14) 220 GTGCAG BsmFI 51 GGGAC GGGAC(10/14) 591 GTCCC BsrDI 69 CATTGC GCAATG(2/0) 273 CATTGC BsrI 475 ACTGG ACTGG(1/-1) 1242 CCAGT HaeII 139 GGCGCC [GA]GCGC^[CT] 998 GGCGCC HgaI 53 GACGC GACGC(5/10) 713 GCGTC Hin1I 139 GGCGCC G[GA]^CG[CT]C 998 GGCGCC HincII 1033 GTCAAC GT[CT]^[GA]AC 1215 GTTGAC HindII 1033 GTCAAC GT[CT]^[GA]AC 1215 GTTGAC KasI 139 GGCGCC G^GCGCC 998 GGCGCC MboII 990 GAAGA GAAGA(8/7) 242 TCTTC NaeI 634 GCCGGC GCC^GGC 851 GCCGGC NarI 139 GGCGCC GG^CGCC 998 GGCGCC NciI 88 CCGGG CC^[GC]GG 585 CCGGG NgoMIV 634 GCCGGC G^CCGGC 851 GCCGGC PflMI 1092 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1242 CCAGTACCTGG PleI 544 GACTC GAGTC(4/5) 589 GAGTC SfoI 139 GGCGCC GGC^GCC 998 GGCGCC TaqI 917 TCGA T^CGA 1109 TCGA TthHB8I 917 TCGA T^CGA 1109 TCGA Van91I 1092 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1242 CCAGTACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MnlI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PshAI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI