(Circular) (Six-base) MAPSORT of: pFN21AE4419.seq Check: 7829 from: 1 to: 6001 pFN21AE4419 6001 bp With 182 enzymes: SgfI * March 28, 2012 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2965 2965 Size: 6001 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3344 1937 Size: 1407 4594 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 3210 4064 5448 5716 1632 Size: 527 1051 854 1384 268 1917 Fragments arranged by size: 1917 1384 1051 854 527 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 2982 4832 2982 Size: 1850 4151 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 2627 1166 Size: 1461 4540 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3032 1184 Size: 1848 4153 AlwNI CAG_nnn'CTG Cuts at: 2356 2443 2698 3188 3253 3744 5316 2356 Size: 87 255 490 65 491 1572 3041 Fragments arranged by size: 3041 1572 491 490 255 87 65 ApaI G_GGCC'C Cuts at: 2376 2376 Size: 6001 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 3310 3536 3688 5797 1493 Size: 1817 226 152 2109 1697 Fragments arranged by size: 2109 1817 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3326 1870 Size: 73 1383 4545 Fragments arranged by size: 4545 1383 73 BamHI G'GATC_C Cuts at: 2517 3331 3698 2517 Size: 814 367 4820 Fragments arranged by size: 4820 814 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2527 3003 3322 4777 Size: 325 217 627 357 383 476 319 1455 Cuts at: 4777 618 Size: 1842 Fragments arranged by size: 1842 1455 627 476 383 357 325 319 217 BanII G_rGCy'C Cuts at: 1391 2376 2716 3320 1391 Size: 985 340 604 4072 Fragments arranged by size: 4072 985 604 340 BbeI G_GCGC'C Cuts at: 2148 3007 2148 Size: 859 5142 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5030 BbvCI CC'TCA_GC Cuts at: 2220 2220 Size: 6001 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4192 4226 1903 Size: 34 2255 34 3678 Fragments arranged by size: 3678 2255 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4612 136 Size: 107 122 71 631 605 2940 1525 Fragments arranged by size: 2940 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 3418 3418 Size: 6001 Bme1580I G_kGCm'C Cuts at: 1790 2376 2692 4062 5218 1790 Size: 586 316 1370 1156 2573 Fragments arranged by size: 2573 1370 1156 586 316 BmgBI CAC'GTC Cuts at: 1318 2914 1318 Size: 1596 4405 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2490 3241 3732 Size: 416 53 83 399 24 1073 751 491 Cuts at: 3732 4690 442 Size: 958 1753 Fragments arranged by size: 1753 1073 958 751 491 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2137 2137 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4661 1239 Size: 3422 2579 Bpu10I CC'TnA_GC Cuts at: 2220 2220 Size: 6001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2507 4126 4991 5289 2507 Size: 1619 865 298 3219 Fragments arranged by size: 3219 1619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2192 2465 4664 882 Size: 617 693 273 2199 2219 Fragments arranged by size: 2219 2199 693 617 273 BsaAI yAC'GTr Cuts at: 493 1204 3792 493 Size: 711 2588 2702 Fragments arranged by size: 2702 2588 711 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 3004 3808 4190 Size: 53 83 186 936 612 859 804 382 Cuts at: 4190 4825 275 Size: 635 1451 Fragments arranged by size: 1451 936 859 804 635 612 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2296 2982 4434 4832 5106 5253 5664 2296 Size: 686 1452 398 274 147 411 2633 Fragments arranged by size: 2633 1452 686 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 2234 2264 2321 Size: 30 165 30 708 30 93 30 57 Cuts at: 2321 2351 2703 2733 1178 Size: 30 352 30 4446 Fragments arranged by size: 4446 708 352 165 93 57 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 2483 1186 Size: 1156 141 4704 Fragments arranged by size: 4704 1156 141 BseYI C'CCAG_C Cuts at: 3065 5204 5700 3065 Size: 2139 496 3366 Fragments arranged by size: 3366 2139 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2246 1163 Size: 573 414 96 4918 Fragments arranged by size: 4918 573 414 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2187 3451 4212 5240 1540 Size: 275 186 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 186 BsiHKAI G_wGCw'C Cuts at: 4062 4147 5218 4062 Size: 85 1071 4845 Fragments arranged by size: 4845 1071 85 BsmI GAATG_Cn' Cuts at: 2292 3512 3605 2292 Size: 1220 93 4688 Fragments arranged by size: 4688 1220 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2174 2174 Size: 6001 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2376 2692 2716 3320 4062 4147 5218 Size: 399 586 316 24 604 742 85 1071 Cuts at: 5218 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 604 586 399 316 85 24 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2965 3356 844 Size: 2121 391 3489 Fragments arranged by size: 3489 2121 391 BsrBI CCG'CTC Cuts at: 1394 1823 2116 3888 1394 Size: 429 293 1772 3507 Fragments arranged by size: 3507 1772 429 293 BsrDI GCAATG_nn' Cuts at: 65 1248 2073 2277 4496 4670 65 Size: 1183 825 204 2219 174 1396 Fragments arranged by size: 2219 1396 1183 825 204 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2639 2856 2982 4645 4832 1700 Size: 110 829 217 126 1663 187 2869 Fragments arranged by size: 2869 1663 829 217 187 126 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 2443 794 Size: 1649 4352 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 2779 1184 Size: 1595 4406 BstXI CCAn_nnnn'nTGG Cuts at: 3172 3172 Size: 6001 BstYI r'GATC_y Cuts at: 1972 2517 3331 3698 4083 4100 5552 5638 5650 Size: 545 814 367 385 17 1452 86 12 Cuts at: 5650 5996 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 814 545 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2175 2763 2787 2949 3054 513 Size: 552 1110 588 24 162 105 3460 Fragments arranged by size: 3460 1110 588 552 162 105 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2137 487 Size: 683 56 911 4351 Fragments arranged by size: 4351 911 683 56 BtsI GCAGTG_nn' Cuts at: 2234 2750 3189 3517 4311 4331 2234 Size: 516 439 328 794 20 3904 Fragments arranged by size: 3904 794 516 439 328 20 ClaI AT'CG_AT Cuts at: 1554 2922 1554 Size: 1368 4633 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2508 2854 Size: 54 1425 50 275 18 354 324 346 Cuts at: 2854 3021 3448 4337 5966 8 Size: 167 427 889 1629 43 Fragments arranged by size: 1629 1425 889 427 354 346 324 275 167 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2184 3448 1537 Size: 275 372 1264 4090 Fragments arranged by size: 4090 1264 372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3931 1353 Size: 2578 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4588 4962 5108 387 Size: 1064 138 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 3210 4064 4661 5448 5716 1239 Size: 393 527 1051 854 597 787 268 1524 Fragments arranged by size: 1524 1051 854 787 597 527 393 268 EcoO109I rG'GnC_Cy Cuts at: 2065 2373 2695 2065 Size: 308 322 5371 Fragments arranged by size: 5371 322 308 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 2148 3007 5148 1678 Size: 470 859 2141 2531 Fragments arranged by size: 2531 2141 859 470 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2703 2735 4642 4674 4716 Size: 32 135 32 1131 32 1907 32 42 Cuts at: 4716 4748 1373 Size: 32 2626 Fragments arranged by size: 2626 1907 1131 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3040 3222 3345 3591 669 Size: 988 281 1102 182 123 246 3079 Fragments arranged by size: 3079 1102 988 281 246 182 123 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KasI G'GCGC_C Cuts at: 2144 3003 2144 Size: 859 5142 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2510 10 Size: 54 1425 343 678 3501 Fragments arranged by size: 3501 1425 678 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2297 2627 3959 4318 4477 518 Size: 648 1008 123 330 1332 359 159 2042 Fragments arranged by size: 2042 1332 1008 648 359 330 159 123 MspA1I CmG'CkG Cuts at: 1606 2238 3417 4094 5242 5487 1606 Size: 632 1179 677 1148 245 2120 Fragments arranged by size: 2120 1179 1148 677 632 245 NaeI GCC'GGC Cuts at: 2641 2858 2641 Size: 217 5784 NarI GG'CG_CC Cuts at: 2145 3004 2145 Size: 859 5142 NcoI C'CATG_G Cuts at: 513 1065 2763 2787 3054 513 Size: 552 1698 24 267 3460 Fragments arranged by size: 3460 1698 552 267 24 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NgoMIV G'CCGG_C Cuts at: 2639 2856 2639 Size: 217 5784 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 3262 3359 4904 1132 Size: 97 2033 97 1545 2229 Fragments arranged by size: 2229 2033 1545 97 97 PasI CC'CwG_GG Cuts at: 1348 2212 1348 Size: 864 5137 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 3103 3253 1108 Size: 1995 150 3856 Fragments arranged by size: 3856 1995 150 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2186 2204 2483 1613 Size: 573 18 279 5131 Fragments arranged by size: 5131 573 279 18 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PspOMI G'GGCC_C Cuts at: 2372 2372 Size: 6001 PstI C_TGCA'G Cuts at: 830 2757 3353 830 Size: 1927 596 3478 Fragments arranged by size: 3478 1927 596 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2238 2238 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SexAI A'CCwGG_T Cuts at: 3096 3096 Size: 6001 SfcI C'TryA_G Cuts at: 826 1045 2753 3349 4484 5165 5356 826 Size: 219 1708 596 1135 681 191 1471 Fragments arranged by size: 1708 1471 1135 681 596 219 191 SfoI GGC'GCC Cuts at: 2146 3005 2146 Size: 859 5142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2522 4105 5006 5268 820 Size: 197 926 579 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 579 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 2798 1517 Size: 1281 4720 StyI C'CwwG_G Cuts at: 513 1065 2283 2763 2787 2851 3054 3440 513 Size: 552 1218 480 24 64 203 386 3074 Fragments arranged by size: 3074 1218 552 480 386 203 64 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2270 4028 4045 4198 4383 4828 Size: 1255 624 66 1758 17 153 185 445 Cuts at: 4828 325 Size: 1498 Fragments arranged by size: 1758 1498 1255 624 445 185 153 66 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2358 4247 5959 Size: 274 80 33 51 1443 381 1889 1712 Cuts at: 5959 96 Size: 138 Fragments arranged by size: 1889 1712 1443 381 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2321 2353 5961 5993 2321 Size: 32 3608 32 2329 Fragments arranged by size: 3608 2329 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2552 1316 Size: 1236 4765 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3100 3100 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 4130 4130 Size: 6001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI