Restriction map for pFN21AE4419
 (Linear) (Six-base) MAPSORT of: KIEE4419.seq  Check: 7041  from: 1  to: 1296



KIEE4419.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     960    1296 
   Size:        960     336
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154    1205    1296 
   Size:        154    1051      91

  Fragments arranged by size: 

                1051     154      91
 
AgeI A'CCGG_T

Cuts at:      0     977    1296 
   Size:        977     319
 
AleI CACnn'nnGTG

Cuts at:      0     622    1296 
   Size:        622     674
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1027    1296 
   Size:       1027     269
 
AlwNI CAG_nnn'CTG

Cuts at:      0     351     438     693    1183    1248    1296 
   Size:        351      87     255     490      65      48

  Fragments arranged by size: 

                 490     351     255      87      65      48
 
ApaI G_GGCC'C

Cuts at:      0     371    1296 
   Size:        371     925
 
BamHI G'GATC_C

Cuts at:      0     512    1296 
   Size:        512     784
 
BanI G'GyrC_C

Cuts at:      0     139     522     998    1296 
   Size:        139     383     476     298

  Fragments arranged by size: 

                 476     383     298     139
 
BanII G_rGCy'C

Cuts at:      0     371     711    1296 
   Size:        371     340     585

  Fragments arranged by size: 

                 585     371     340
 
BbeI G_GCGC'C

Cuts at:      0     143    1002    1296 
   Size:        143     859     294

  Fragments arranged by size: 

                 859     294     143
 
BbvCI CC'TCA_GC

Cuts at:      0     215    1296 
   Size:        215    1081
 
Bme1580I G_kGCm'C

Cuts at:      0     371     687    1296 
   Size:        371     316     609

  Fragments arranged by size: 

                 609     371     316
 
BmgBI CAC'GTC

Cuts at:      0     909    1296 
   Size:        909     387
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     485    1236    1296 
   Size:        485     751      60

  Fragments arranged by size: 

                 751     485      60
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     132    1296 
   Size:        132    1164
 
Bpu10I CC'TnA_GC

Cuts at:      0     215    1296 
   Size:        215    1081
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     502    1296 
   Size:        502     794
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     187     460    1296 
   Size:        187     273     836

  Fragments arranged by size: 

                 836     273     187
 
BsaHI Gr'CG_yC

Cuts at:      0     140     999    1296 
   Size:        140     859     297

  Fragments arranged by size: 

                 859     297     140
 
BsaWI w'CCGG_w

Cuts at:      0     291     977    1296 
   Size:        291     686     319

  Fragments arranged by size: 

                 686     319     291
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     106     136     229     259     316     346     698     728 
   Size:        106      30      93      30      57      30     352      30

Cuts at:    728    1296 
   Size:        568

  Fragments arranged by size: 

                 568     352     106      93      57      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     337     478    1296 
   Size:        337     141     818

  Fragments arranged by size: 

                 818     337     141
 
BseYI C'CCAG_C

Cuts at:      0    1060    1296 
   Size:       1060     236
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     241    1296 
   Size:        145      96    1055

  Fragments arranged by size: 

                1055     145      96
 
BsiEI CG_ry'CG

Cuts at:      0     182    1296 
   Size:        182    1114
 
BsmI GAATG_Cn'

Cuts at:      0     287    1296 
   Size:        287    1009
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     169    1296 
   Size:        169    1127
 
Bsp1286I G_dGCh'C

Cuts at:      0     371     687     711    1296 
   Size:        371     316      24     585

  Fragments arranged by size: 

                 585     371     316      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     960    1296 
   Size:        960     336
 
BsrBI CCG'CTC

Cuts at:      0     111    1296 
   Size:        111    1185
 
BsrDI GCAATG_nn'

Cuts at:      0      68     272    1296 
   Size:         68     204    1024

  Fragments arranged by size: 

                1024     204      68
 
BsrFI r'CCGG_y

Cuts at:      0     634     851     977    1296 
   Size:        634     217     126     319

  Fragments arranged by size: 

                 634     319     217     126
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     438    1296 
   Size:        438     858
 
BstEII G'GTnAC_C

Cuts at:      0     774    1296 
   Size:        774     522
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1167    1296 
   Size:       1167     129
 
BstYI r'GATC_y

Cuts at:      0     512    1296 
   Size:        512     784
 
BtgI C'CryG_G

Cuts at:      0     170     758     782     944    1049    1296 
   Size:        170     588      24     162     105     247

  Fragments arranged by size: 

                 588     247     170     162     105      24
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     132    1296 
   Size:        132    1164
 
BtsI GCAGTG_nn'

Cuts at:      0     229     745    1184    1296 
   Size:        229     516     439     112

  Fragments arranged by size: 

                 516     439     229     112
 
ClaI AT'CG_AT

Cuts at:      0     917    1296 
   Size:        917     379
 
EaeI y'GGCC_r

Cuts at:      0     179     503     849    1016    1296 
   Size:        179     324     346     167     280

  Fragments arranged by size: 

                 346     324     280     179     167
 
EagI C'GGCC_G

Cuts at:      0     179    1296 
   Size:        179    1117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154    1205    1296 
   Size:        154    1051      91

  Fragments arranged by size: 

                1051     154      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60     368     690    1296 
   Size:         60     308     322     606

  Fragments arranged by size: 

                 606     322     308      60
 
HaeII r_GCGC'y

Cuts at:      0     143    1002    1296 
   Size:        143     859     294

  Fragments arranged by size: 

                 859     294     143
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     698     730    1296 
   Size:        698      32     566

  Fragments arranged by size: 

                 698     566      32
 
HincII GTy'rAC

Cuts at:      0    1035    1217    1296 
   Size:       1035     182      79

  Fragments arranged by size: 

                1035     182      79
 
KasI G'GCGC_C

Cuts at:      0     139     998    1296 
   Size:        139     859     298

  Fragments arranged by size: 

                 859     298     139
 
MscI TGG'CCA

Cuts at:      0     505    1296 
   Size:        505     791
 
MslI CAynn'nnrTG

Cuts at:      0     169     292     622    1296 
   Size:        169     123     330     674

  Fragments arranged by size: 

                 674     330     169     123
 
MspA1I CmG'CkG

Cuts at:      0     233    1296 
   Size:        233    1063
 
NaeI GCC'GGC

Cuts at:      0     636     853    1296 
   Size:        636     217     443

  Fragments arranged by size: 

                 636     443     217
 
NarI GG'CG_CC

Cuts at:      0     140     999    1296 
   Size:        140     859     297

  Fragments arranged by size: 

                 859     297     140
 
NcoI C'CATG_G

Cuts at:      0     758     782    1049    1296 
   Size:        758      24     267     247

  Fragments arranged by size: 

                 758     267     247      24
 
NgoMIV G'CCGG_C

Cuts at:      0     634     851    1296 
   Size:        634     217     445

  Fragments arranged by size: 

                 634     445     217
 
NspI r_CATG'y

Cuts at:      0    1257    1296 
   Size:       1257      39
 
PasI CC'CwG_GG

Cuts at:      0     207    1296 
   Size:        207    1089
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1098    1248    1296 
   Size:       1098     150      48

  Fragments arranged by size: 

                1098     150      48
 
PshAI GACnn'nnGTC

Cuts at:      0     181     199     478    1296 
   Size:        181      18     279     818

  Fragments arranged by size: 

                 818     279     181      18
 
PspOMI G'GGCC_C

Cuts at:      0     367    1296 
   Size:        367     929
 
PstI C_TGCA'G

Cuts at:      0     752    1296 
   Size:        752     544
 
PvuII CAG'CTG

Cuts at:      0     233    1296 
   Size:        233    1063
 
SexAI A'CCwGG_T

Cuts at:      0    1091    1296 
   Size:       1091     205
 
SfcI C'TryA_G

Cuts at:      0     748    1296 
   Size:        748     548
 
SfoI GGC'GCC

Cuts at:      0     141    1000    1296 
   Size:        141     859     296

  Fragments arranged by size: 

                 859     296     141
 
SmlI C'TyrA_G

Cuts at:      0     517    1296 
   Size:        517     779
 
StuI AGG'CCT

Cuts at:      0     793    1296 
   Size:        793     503
 
StyI C'CwwG_G

Cuts at:      0     278     758     782     846    1049    1296 
   Size:        278     480      24      64     203     247

  Fragments arranged by size: 

                 480     278     247     203      64      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     199     265    1296 
   Size:        199      66    1031

  Fragments arranged by size: 

                1031     199      66
 
TatI w'GTAC_w

Cuts at:      0     353    1296 
   Size:        353     943
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     316     348    1296 
   Size:        316      32     948

  Fragments arranged by size: 

                 948     316      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     547    1296 
   Size:        547     749
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1095    1296 
   Size:       1095     201
 

 Enzymes that do cut:

     AarI     AcuI     AgeI     AleI     AlfI    AlwNI     ApaI    BamHI
     BanI    BanII     BbeI    BbvCI Bme1580I    BmgBI     BmrI     BplI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI     EaeI     EagI  Eco57MI EcoO109I    HaeII    Hin4I   HincII
     KasI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI    PflMI    PshAI   PspOMI     PstI    PvuII    SexAI
     SfcI     SfoI     SmlI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmtI     BpmI    BsaAI    BsaBI  BsiHKAI    BsiWI    BspEI
    BspHI    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI     XmaI
     XmnI     ZraI
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