################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I MaeII MamI MfeI MluI MroI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspBI SspI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AlwNI 1094 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 804 GAGCTC G[AT]GC[AT]^C AvaI 13 CCCGAG C^[CT]CG[GA]G BamHI 849 GGATCC G^GATCC BbsI 226 GTCTTC GAAGAC(2/6) BbvCI 1160 CCTCAGC CCTCAGC(-5/-2) BfaI 454 CTAG C^TAG BpuAI 226 GTCTTC GAAGAC(2/6) BsaI 986 GAGACC GGTCTC(1/5) BsgI 19 CTGCAC GTGCAG(16/14) BsiEI 360 CGGCCG CG[GA][CT]^CG BsiHKAI 804 GAGCTC G[AT]GC[AT]^C BsmAI 986 GAGAC GTCTC(1/5) BsoBI 13 CCCGAG C^[CT]CG[GA]G BstXI 698 CCAAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 688 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 360 CGGCCG C^GGCCG EclXI 360 CGGCCG C^GGCCG Eco52I 360 CGGCCG C^GGCCG EcoNI 1032 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 57 GGCGCT [GA]GCGC^[CT] HgaI 937 GACGC GACGC(5/10) MaeI 454 CTAG C^TAG MaeIII 686 GTCAC ^GT[ACGT]AC PleI 915 GAGTC GAGTC(4/5) SacI 804 GAGCTC GAGCT^C SfaNI 137 GCATC GCATC(5/9) SphI 791 GCATGC GCATG^C TaqI 768 TCGA T^CGA TfiI 663 GAATC G^A[AT]TC Tsp45I 686 GTCAC ^GT[GC]AC TthHB8I 768 TCGA T^CGA XcmI 843 CCAGGGGGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BalI 26 TGGCCA TGG^CCA 1101 TGGCCA BglI 49 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 353 GCCGGTTCGGC BspMI 293 ACCTGC ACCTGC(4/8) 671 ACCTGC BsrBI 582 CCGCTC CCGCTC(-3/-3) 741 GAGCGG DsaI 153 CCGCGG C^C[GA][CT]GG 396 CCGCGG HinfI 663 GAATC G^A[ACGT]TC 915 GAGTC KspI 153 CCGCGG CCGC^GG 396 CCGCGG MluNI 26 TGGCCA TGG^CCA 1101 TGGCCA MscI 26 TGGCCA TGG^CCA 1101 TGGCCA NspI 239 GCATGT [GA]CATG^[CT] 791 GCATGC PvuII 256 CAGCTG CAG^CTG 910 CAGCTG SacII 153 CCGCGG CCGC^GG 396 CCGCGG SbfI 50 CCTGCAGG CCTGCA^GG 810 CCTGCAGG Sse8387I 50 CCTGCAGG CCTGCA^GG 810 CCTGCAGG StuI 47 AGGCCT AGG^CCT 464 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AocI ApaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI TspRI XhoII