(Circular) (Six-base) MAPSORT of: pFN21AB6239.seq Check: 3634 from: 1 to: 6958 pFN21AB6239 6958 bp With 182 enzymes: SgfI * February 2, 2010 11:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4768 278 Size: 53 83 186 936 3232 2468 Fragments arranged by size: 3232 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3409 4301 1937 Size: 1472 892 4594 Fragments arranged by size: 4594 1472 892 Acc65I G'GTAC_C Cuts at: 4279 4279 Size: 6958 AclI AA'CG_TT Cuts at: 1563 5085 5458 1563 Size: 3522 373 3063 Fragments arranged by size: 3522 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2304 2604 3264 3345 4241 5021 6405 6673 Size: 672 300 660 81 896 780 1384 268 Cuts at: 6673 1632 Size: 1917 Fragments arranged by size: 1917 1384 896 780 672 660 300 268 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6761 AflIII A'CryG_T Cuts at: 1203 3404 4002 5857 6834 1203 Size: 2201 598 1855 977 1327 Fragments arranged by size: 2201 1855 1327 977 598 AgeI A'CCGG_T Cuts at: 3507 5789 3507 Size: 2282 4676 AhdI GACnn_n'nnGTC Cuts at: 5687 5687 Size: 6958 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6958 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6958 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2742 2774 2742 Size: 32 6926 AlwNI CAG_nnn'CTG Cuts at: 3176 4701 6273 3176 Size: 1525 1572 3861 Fragments arranged by size: 3861 1572 1525 ApaLI G'TGCA_C Cuts at: 2207 5015 6171 2207 Size: 2808 1156 2994 Fragments arranged by size: 2994 2808 1156 ApoI r'AATT_y Cuts at: 1493 2148 2359 2511 2654 2670 3250 3640 3926 Size: 655 211 152 143 16 580 390 286 Cuts at: 3926 4068 4267 4493 4645 6754 1493 Size: 142 199 226 152 2109 1697 Fragments arranged by size: 2109 1697 655 580 390 286 226 211 199 152 152 143 142 16 AseI AT'TA_AT Cuts at: 160 1032 3129 5512 160 Size: 872 2097 2383 1606 Fragments arranged by size: 2383 2097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 AvaI C'yCGr_G Cuts at: 1870 1943 4283 1870 Size: 73 2340 4545 Fragments arranged by size: 4545 2340 73 BamHI G'GATC_C Cuts at: 4288 4655 4288 Size: 367 6591 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3868 4279 5734 618 Size: 325 217 627 2081 411 1455 1842 Fragments arranged by size: 2081 1842 1455 627 411 325 217 BanII G_rGCy'C Cuts at: 1391 2231 3239 3848 4277 1391 Size: 840 1008 609 429 4072 Fragments arranged by size: 4072 1008 840 609 429 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5987 BbvCI CC'TCA_GC Cuts at: 3465 3465 Size: 6958 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4068 4102 5149 5183 1903 Size: 34 2131 34 1047 34 3678 Fragments arranged by size: 3678 2131 1047 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2190 3142 3469 4850 6060 2190 Size: 952 327 1381 1210 3088 Fragments arranged by size: 3088 1381 1210 952 327 BclI T'GATC_A Cuts at: 1548 3589 4132 1548 Size: 2041 543 4374 Fragments arranged by size: 4374 2041 543 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5569 136 Size: 107 122 71 631 605 3897 1525 Fragments arranged by size: 3897 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2608 6953 2608 Size: 4345 2613 BlpI GC'TnA_GC Cuts at: 3039 4375 3039 Size: 1336 5622 Bme1580I G_kGCm'C Cuts at: 1790 2211 2711 3623 3734 5019 6175 1790 Size: 421 500 912 111 1285 1156 2573 Fragments arranged by size: 2573 1285 1156 912 500 421 111 BmgBI CAC'GTC Cuts at: 1318 3880 1318 Size: 2562 4396 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2025 4689 5647 Size: 416 53 83 399 24 608 2664 958 Cuts at: 5647 442 Size: 1753 Fragments arranged by size: 2664 1753 958 608 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6958 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2633 3438 5618 1239 Size: 1394 805 2180 2579 Fragments arranged by size: 2579 2180 1394 805 Bpu10I CC'TnA_GC Cuts at: 3465 3465 Size: 6958 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2075 3945 5083 5948 6246 2075 Size: 1870 1138 865 298 2787 Fragments arranged by size: 2787 1870 1138 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2349 5621 882 Size: 617 850 3272 2219 Fragments arranged by size: 3272 2219 850 617 BsaAI yAC'GTr Cuts at: 493 1204 4749 493 Size: 711 3545 2702 Fragments arranged by size: 3545 2702 711 BsaBI GATnn'nnATC Cuts at: 3899 6952 3899 Size: 3053 3905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4765 5147 5782 275 Size: 53 83 186 936 3232 382 635 1451 Fragments arranged by size: 3232 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3507 5391 5789 6063 6210 6621 3507 Size: 1884 398 274 147 411 3844 Fragments arranged by size: 3844 1884 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2400 2430 2742 2772 1178 Size: 30 165 30 997 30 312 30 5364 Fragments arranged by size: 5364 997 312 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3229 3471 3547 1186 Size: 2043 242 76 4597 Fragments arranged by size: 4597 2043 242 76 BseYI C'CCAG_C Cuts at: 3113 3888 6161 6657 3113 Size: 775 2273 496 3414 Fragments arranged by size: 3414 2273 775 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3504 3935 4027 1163 Size: 573 1768 431 92 4094 Fragments arranged by size: 4094 1768 573 431 92 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4408 5169 6197 1540 Size: 275 186 2407 761 1028 2301 Fragments arranged by size: 2407 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2231 3239 5019 5104 6175 2211 Size: 20 1008 1780 85 1071 2994 Fragments arranged by size: 2994 1780 1071 1008 85 20 BsmI GAATG_Cn' Cuts at: 2314 2536 4469 4562 2314 Size: 222 1933 93 4710 Fragments arranged by size: 4710 1933 222 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2568 2568 Size: 6958 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2231 2711 3239 3623 3734 3848 Size: 399 421 20 480 528 384 111 114 Cuts at: 3848 4277 5019 5104 6175 1391 Size: 429 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 528 480 429 421 399 384 114 111 85 20 BspEI T'CCGG_A Cuts at: 6621 6621 Size: 6958 BspHI T'CATG_A Cuts at: 4847 6577 4847 Size: 1730 5228 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4313 844 Size: 3469 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3043 4845 1394 Size: 429 1220 1802 3507 Fragments arranged by size: 3507 1802 1220 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2231 3378 5453 5627 65 Size: 1183 983 1147 2075 174 1396 Fragments arranged by size: 2075 1396 1183 1147 983 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3507 5602 5789 1700 Size: 110 1697 2095 187 2869 Fragments arranged by size: 2869 2095 1697 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6958 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6958 BssSI C'ACGA_G Cuts at: 5018 6030 5018 Size: 1012 5946 BstAPI GCAn_nnn'nTGC Cuts at: 794 3828 3862 794 Size: 3034 34 3890 Fragments arranged by size: 3890 3034 34 BstBI TT'CG_AA Cuts at: 5762 5762 Size: 6958 BstEII G'GTnAC_C Cuts at: 1184 2257 1184 Size: 1073 5885 BstXI CCAn_nnnn'nTGG Cuts at: 2046 2269 2046 Size: 223 6735 BstYI r'GATC_y Cuts at: 1972 2608 3907 4288 4655 5040 5057 6509 6595 Size: 636 1299 381 367 385 17 1452 86 Cuts at: 6595 6607 6953 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1299 636 385 381 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3596 513 Size: 552 939 1592 3875 Fragments arranged by size: 3875 1592 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2881 487 Size: 683 56 1655 4564 Fragments arranged by size: 4564 1655 683 56 BtsI GCAGTG_nn' Cuts at: 3523 3734 4474 5268 5288 3523 Size: 211 740 794 20 5193 Fragments arranged by size: 5193 794 740 211 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6958 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2959 2994 529 Size: 35 2395 35 4493 Fragments arranged by size: 4493 2395 35 35 DraI TTT'AAA Cuts at: 2875 4262 2875 Size: 1387 5571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5965 809 Size: 507 4649 1802 Fragments arranged by size: 4649 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4405 5294 6923 Size: 54 1425 50 275 18 2575 889 1629 Cuts at: 6923 8 Size: 43 Fragments arranged by size: 2575 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4405 1537 Size: 275 2593 4090 Fragments arranged by size: 4090 2593 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3076 3207 3491 3973 4173 4888 1353 Size: 1723 131 284 482 200 715 3423 Fragments arranged by size: 3423 1723 715 482 284 200 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 5545 5919 6065 387 Size: 1064 138 476 3480 374 146 1280 Fragments arranged by size: 3480 1280 1064 476 374 146 138 EcoICRI GAG'CTC Cuts at: 2229 3237 2229 Size: 1008 5950 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2304 2604 2633 3264 3345 3438 4241 Size: 393 672 300 29 631 81 93 803 Cuts at: 4241 5021 5618 6405 6673 1239 Size: 780 597 787 268 1524 Fragments arranged by size: 1524 803 787 780 672 631 597 393 300 268 93 81 29 EcoRI G'AATT_C Cuts at: 3926 4267 4645 3926 Size: 341 378 6239 Fragments arranged by size: 6239 378 341 FspI TGC'GCA Cuts at: 5464 5464 Size: 6958 HaeII r_GCGC'y Cuts at: 1678 6105 1678 Size: 4427 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3542 3574 3943 3975 5599 Size: 32 135 32 1970 32 369 32 1624 Cuts at: 5599 5631 5673 5705 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1970 1624 369 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4126 4302 4548 669 Size: 988 281 2188 176 246 3079 Fragments arranged by size: 3079 2188 988 281 246 176 HindIII A'AGCT_T Cuts at: 748 2833 748 Size: 2085 4873 HpaI GTT'AAC Cuts at: 4126 4548 4126 Size: 422 6536 KpnI G_GTAC'C Cuts at: 4283 4283 Size: 6958 MfeI C'AATT_G Cuts at: 4557 4557 Size: 6958 MluI A'CGCG_T Cuts at: 6834 6834 Size: 6958 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2303 6072 6256 1305 Size: 998 3769 184 2007 Fragments arranged by size: 3769 2007 998 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5136 Fragments arranged by size: 5136 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2625 3131 4916 5275 5434 518 Size: 648 1459 506 1785 359 159 2042 Fragments arranged by size: 2042 1785 1459 648 506 359 159 MspA1I CmG'CkG Cuts at: 1606 3038 3464 3541 3702 3791 3841 4374 5051 Size: 1432 426 77 161 89 50 533 677 Cuts at: 5051 6199 6444 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1432 1148 677 533 426 245 161 89 77 50 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5467 Fragments arranged by size: 5467 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6958 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6958 NotI GC'GGCC_GC Cuts at: 4405 4405 Size: 6958 NspI r_CATG'y Cuts at: 1132 1229 2077 3408 4006 4316 5861 1132 Size: 97 848 1331 598 310 1545 2229 Fragments arranged by size: 2229 1545 1331 848 598 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6958 PciI A'CATG_T Cuts at: 3404 4002 5857 3404 Size: 598 1855 4505 Fragments arranged by size: 4505 1855 598 PflMI CCAn_nnn'nTGG Cuts at: 1108 3001 1108 Size: 1893 5065 PfoI T'CCnGG_A Cuts at: 3067 3691 3067 Size: 624 6334 PmeI GTTT'AAAC Cuts at: 4262 4262 Size: 6958 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2742 2774 5027 5059 6540 6572 2742 Size: 32 2253 32 1481 32 3128 Fragments arranged by size: 3128 2253 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3771 1613 Size: 2158 4800 PsiI TTA'TAA Cuts at: 2501 2937 4528 2501 Size: 436 1591 4931 Fragments arranged by size: 4931 1591 436 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2116 2148 2116 Size: 32 6926 PstI C_TGCA'G Cuts at: 830 3530 3833 4310 830 Size: 2700 303 477 3478 Fragments arranged by size: 3478 2700 477 303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6958 PvuII CAG'CTG Cuts at: 3541 3541 Size: 6958 SacI G_AGCT'C Cuts at: 2231 3239 2231 Size: 1008 5950 SalI G'TCGA_C Cuts at: 1936 4300 1936 Size: 2364 4594 SapI GCTCTTCn'nnn_ Cuts at: 4173 4173 Size: 6958 SbfI CC_TGCA'GG Cuts at: 4310 4310 Size: 6958 ScaI AGT'ACT Cuts at: 5206 6918 5206 Size: 1712 5246 SfcI C'TryA_G Cuts at: 826 1045 2351 2860 3526 3829 4306 5441 6122 Size: 219 1306 509 666 303 477 1135 681 Cuts at: 6122 6313 826 Size: 191 1471 Fragments arranged by size: 1471 1306 1135 681 666 509 477 303 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6958 SmaI CCC'GGG Cuts at: 1872 4285 1872 Size: 2413 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 3960 5062 5963 6225 820 Size: 197 926 147 1870 1102 901 262 1553 Fragments arranged by size: 1870 1553 1102 926 901 262 197 147 SnaBI TAC'GTA Cuts at: 493 4749 493 Size: 4256 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6958 SphI G_CATG'C Cuts at: 1132 4316 1132 Size: 3184 3774 SspI AAT'ATT Cuts at: 5 52 3289 4882 5 Size: 47 3237 1593 2081 Fragments arranged by size: 3237 2081 1593 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6958 StyI C'CwwG_G Cuts at: 513 1065 2004 2536 3485 4397 513 Size: 552 939 532 949 912 3074 Fragments arranged by size: 3074 949 939 912 552 532 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2941 4985 5002 5155 5340 5785 325 Size: 1255 1361 2044 17 153 185 445 1498 Fragments arranged by size: 2044 1498 1361 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2070 2473 5204 Size: 274 80 33 51 1443 93 403 2731 Cuts at: 5204 6916 96 Size: 1712 138 Fragments arranged by size: 2731 1712 1443 403 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 6918 6950 2274 Size: 32 4612 32 2282 Fragments arranged by size: 4612 2282 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6958 XbaI T'CTAG_A Cuts at: 2962 4294 2962 Size: 1332 5626 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3133 3133 Size: 6958 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6958 XmaI C'CCGG_G Cuts at: 1870 4283 1870 Size: 2413 4545 XmnI GAAnn'nnTTC Cuts at: 5087 5087 Size: 6958 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4766 276 Size: 53 83 186 936 3232 2468 Fragments arranged by size: 3232 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI