Restriction map for pFN21AB6239
 (Linear) (Six-base) MAPSORT of: KIBB6239.seq  Check: 4431  from: 1  to: 2253



KIBB6239.orf

 With 182 enzymes: SgfI * 

                        January 18, 2013 16:22  ..

AccI GT'mk_AC

Cuts at:      0    1404    2253 
   Size:       1404     849
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     599    1259    1340    2236    2253 
   Size:        299     300     660      81     896      17

  Fragments arranged by size: 

                 896     660     300     299      81      17
 
AflIII A'CryG_T

Cuts at:      0    1399    1997    2253 
   Size:       1399     598     256

  Fragments arranged by size: 

                1399     598     256
 
AgeI A'CCGG_T

Cuts at:      0    1502    2253 
   Size:       1502     751
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     737     769    2253 
   Size:        737      32    1484

  Fragments arranged by size: 

                1484     737      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1171    2253 
   Size:       1171    1082
 
ApaLI G'TGCA_C

Cuts at:      0     202    2253 
   Size:        202    2051
 
ApoI r'AATT_y

Cuts at:      0     143     354     506     649     665    1245    1635    1921 
   Size:        143     211     152     143      16     580     390     286

Cuts at:   1921    2063    2253 
   Size:        142     190

  Fragments arranged by size: 

                 580     390     286     211     190     152     143     143
                 142      16
 
AseI AT'TA_AT

Cuts at:      0    1124    2253 
   Size:       1124    1129
 
BanI G'GyrC_C

Cuts at:      0    1863    2253 
   Size:       1863     390
 
BanII G_rGCy'C

Cuts at:      0     226    1234    1843    2253 
   Size:        226    1008     609     410

  Fragments arranged by size: 

                1008     609     410     226
 
BbvCI CC'TCA_GC

Cuts at:      0    1460    2253 
   Size:       1460     793
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2063    2097    2253 
   Size:       2063      34     156

  Fragments arranged by size: 

                2063     156      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     185    1137    1464    2253 
   Size:        185     952     327     789

  Fragments arranged by size: 

                 952     789     327     185
 
BclI T'GATC_A

Cuts at:      0    1584    2127    2253 
   Size:       1584     543     126

  Fragments arranged by size: 

                1584     543     126
 
BglII A'GATC_T

Cuts at:      0     603    2253 
   Size:        603    1650
 
BlpI GC'TnA_GC

Cuts at:      0    1034    2253 
   Size:       1034    1219
 
Bme1580I G_kGCm'C

Cuts at:      0     206     706    1618    1729    2253 
   Size:        206     500     912     111     524

  Fragments arranged by size: 

                 912     524     500     206     111
 
BmgBI CAC'GTC

Cuts at:      0    1875    2253 
   Size:       1875     378
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      20    2253 
   Size:         20    2233
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628    1433    2253 
   Size:        628     805     820

  Fragments arranged by size: 

                 820     805     628
 
Bpu10I CC'TnA_GC

Cuts at:      0    1460    2253 
   Size:       1460     793
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70    1940    2253 
   Size:         70    1870     313

  Fragments arranged by size: 

                1870     313      70
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     344    2253 
   Size:        344    1909
 
BsaBI GATnn'nnATC

Cuts at:      0    1894    2253 
   Size:       1894     359
 
BsaWI w'CCGG_w

Cuts at:      0    1502    2253 
   Size:       1502     751
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     395     425     737     767    2253 
   Size:        395      30     312      30    1486

  Fragments arranged by size: 

                1486     395     312      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1224    1466    1542    2253 
   Size:       1224     242      76     711

  Fragments arranged by size: 

                1224     711     242      76
 
BseYI C'CCAG_C

Cuts at:      0    1108    1883    2253 
   Size:       1108     775     370

  Fragments arranged by size: 

                1108     775     370
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1499    1930    2022    2253 
   Size:       1499     431      92     231

  Fragments arranged by size: 

                1499     431     231      92
 
BsiHKAI G_wGCw'C

Cuts at:      0     206     226    1234    2253 
   Size:        206      20    1008    1019

  Fragments arranged by size: 

                1019    1008     206      20
 
BsmI GAATG_Cn'

Cuts at:      0     309     531    2253 
   Size:        309     222    1722

  Fragments arranged by size: 

                1722     309     222
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     563    2253 
   Size:        563    1690
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     226     706    1234    1618    1729    1843    2253 
   Size:        206      20     480     528     384     111     114     410

  Fragments arranged by size: 

                 528     480     410     384     206     114     111      20
            
 
BsrBI CCG'CTC

Cuts at:      0    1038    2253 
   Size:       1038    1215
 
BsrDI GCAATG_nn'

Cuts at:      0     226    1373    2253 
   Size:        226    1147     880

  Fragments arranged by size: 

                1147     880     226
 
BsrFI r'CCGG_y

Cuts at:      0    1502    2253 
   Size:       1502     751
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1823    1857    2253 
   Size:       1823      34     396

  Fragments arranged by size: 

                1823     396      34
 
BstEII G'GTnAC_C

Cuts at:      0     252    2253 
   Size:        252    2001
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      41     264    2253 
   Size:         41     223    1989

  Fragments arranged by size: 

                1989     223      41
 
BstYI r'GATC_y

Cuts at:      0     603    1902    2253 
   Size:        603    1299     351

  Fragments arranged by size: 

                1299     603     351
 
BtgI C'CryG_G

Cuts at:      0    1591    2253 
   Size:       1591     662
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     876    2253 
   Size:        876    1377
 
BtsI GCAGTG_nn'

Cuts at:      0    1518    1729    2253 
   Size:       1518     211     524

  Fragments arranged by size: 

                1518     524     211
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     954     989    2253 
   Size:        954      35    1264

  Fragments arranged by size: 

                1264     954      35
 
DraI TTT'AAA

Cuts at:      0     870    2253 
   Size:        870    1383
 
EarI CTCTTCn'nnn_

Cuts at:      0    1071    1202    1486    1968    2168    2253 
   Size:       1071     131     284     482     200      85

  Fragments arranged by size: 

                1071     482     284     200     131      85
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      60    2253 
   Size:         60    2193
 
EcoICRI GAG'CTC

Cuts at:      0     224    1232    2253 
   Size:        224    1008    1021

  Fragments arranged by size: 

                1021    1008     224
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     599     628    1259    1340    1433    2236    2253 
   Size:        299     300      29     631      81      93     803      17

  Fragments arranged by size: 

                 803     631     300     299      93      81      29      17
            
 
EcoRI G'AATT_C

Cuts at:      0    1921    2253 
   Size:       1921     332
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1537    1569    1938    1970    2253 
   Size:       1537      32     369      32     283

  Fragments arranged by size: 

                1537     369     283      32      32
 
HincII GTy'rAC

Cuts at:      0    2121    2253 
   Size:       2121     132
 
HindIII A'AGCT_T

Cuts at:      0     828    2253 
   Size:        828    1425
 
HpaI GTT'AAC

Cuts at:      0    2121    2253 
   Size:       2121     132
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     298    2253 
   Size:        298    1955
 
MslI CAynn'nnrTG

Cuts at:      0     620    1126    2253 
   Size:        620     506    1127

  Fragments arranged by size: 

                1127     620     506
 
MspA1I CmG'CkG

Cuts at:      0    1033    1459    1536    1697    1786    1836    2253 
   Size:       1033     426      77     161      89      50     417

  Fragments arranged by size: 

                1033     426     417     161      89      77      50
 
NspI r_CATG'y

Cuts at:      0      72    1403    2001    2253 
   Size:         72    1331     598     252

  Fragments arranged by size: 

                1331     598     252      72
 
PciI A'CATG_T

Cuts at:      0    1399    1997    2253 
   Size:       1399     598     256

  Fragments arranged by size: 

                1399     598     256
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     996    2253 
   Size:        996    1257
 
PfoI T'CCnGG_A

Cuts at:      0    1062    1686    2253 
   Size:       1062     624     567

  Fragments arranged by size: 

                1062     624     567
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     737     769    2253 
   Size:        737      32    1484

  Fragments arranged by size: 

                1484     737      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1766    2253 
   Size:       1766     487
 
PsiI TTA'TAA

Cuts at:      0     496     932    2253 
   Size:        496     436    1321

  Fragments arranged by size: 

                1321     496     436
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     111     143    2253 
   Size:        111      32    2110

  Fragments arranged by size: 

                2110     111      32
 
PstI C_TGCA'G

Cuts at:      0    1525    1828    2253 
   Size:       1525     303     425

  Fragments arranged by size: 

                1525     425     303
 
PvuII CAG'CTG

Cuts at:      0    1536    2253 
   Size:       1536     717
 
SacI G_AGCT'C

Cuts at:      0     226    1234    2253 
   Size:        226    1008    1019

  Fragments arranged by size: 

                1019    1008     226
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2168    2253 
   Size:       2168      85
 
SfcI C'TryA_G

Cuts at:      0     346     855    1521    1824    2253 
   Size:        346     509     666     303     429

  Fragments arranged by size: 

                 666     509     429     346     303
 
SmlI C'TyrA_G

Cuts at:      0      85    1955    2253 
   Size:         85    1870     298

  Fragments arranged by size: 

                1870     298      85
 
SspI AAT'ATT

Cuts at:      0    1284    2253 
   Size:       1284     969
 
StyI C'CwwG_G

Cuts at:      0     531    1480    2253 
   Size:        531     949     773

  Fragments arranged by size: 

                 949     773     531
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     936    2253 
   Size:        936    1317
 
TatI w'GTAC_w

Cuts at:      0      65     468    2253 
   Size:         65     403    1785

  Fragments arranged by size: 

                1785     403      65
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     269     301    2253 
   Size:        269      32    1952

  Fragments arranged by size: 

                1952     269      32
 
XbaI T'CTAG_A

Cuts at:      0     957    2253 
   Size:        957    1296
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1128    2253 
   Size:       1128    1125
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AgeI     AloI    AlwNI    ApaLI     ApoI
     AseI     BanI    BanII    BbvCI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI   BstAPI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI    Hin4I   HincII  HindIII     HpaI     MmeI
     MslI   MspA1I     NspI     PciI    PflMI     PfoI     PpiI    PshAI
     PsiI     PsrI     PstI    PvuII     SacI     SapI     SfcI     SmlI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI    BfrBI     BglI     BmtI     BplI    BsaAI    BsaHI
    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI
    BstBI  BstZ17I   Bsu36I     ClaI   DraIII     DrdI     EaeI     EagI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI    PpuMI   PspOMI    PspXI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com