################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaI BsaWI BseAI BsiEI BsiHKAI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KspI MaeI MfeI MluI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Tru9I Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 665 GGTACC G^GTACC Aor51HI 97 AGCGCT AGC^GCT Asp718I 665 GGTACC G^GTACC AspI 391 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 308 GGTCC G^G[AT]CC BanII 892 GAGCCC G[GA]GC[CT]^C BbeI 61 GGCGCC GGCGC^C BglII 686 AGATCT A^GATCT BmyI 892 GAGCCC G[AGT]GC[ACT]^C BsaBI 687 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 789 CTCCTC GAGGAG(10/8) BsgI 736 CTGCAC GTGCAG(16/14) BsiWI 338 CGTACG C^GTACG BsmAI 478 GAGAC GTCTC(1/5) BsmBI 478 GAGACG CGTCTC(1/5) Bsp1286I 892 GAGCCC G[AGT]GC[ACT]^C BsrBI 304 GAGCGG CCGCTC(-3/-3) BsrDI 189 CATTGC GCAATG(2/0) BssHII 50 GCGCGC G^CGCGC BstEII 672 GGTCACC G^GT[ACGT]ACC BstPI 672 GGTCACC G^GT[ACGT]ACC BstYI 686 AGATCT [GA]^GATC[CT] DraII 621 AGGGCCT [GA]G^G[ACGT]CC[CT] Eco47III 97 AGCGCT AGC^GCT EcoO109I 621 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 672 GGTCACC G^GT[ACGT]ACC HincII 397 GTCAAC GT[CT]^[GA]AC HindII 397 GTCAAC GT[CT]^[GA]AC KasI 61 GGCGCC G^GCGCC KpnI 665 GGTACC GGTAC^C MamI 687 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 686 AGATCT [GA]^GATC[CT] NarI 61 GGCGCC GG^CGCC NspI 536 GCATGT [GA]CATG^[CT] PaeR7I 211 CTCGAG C^TCGAG PflFI 391 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 378 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 55 CAGCTG CAG^CTG SapI 411 GCTCTTC GCTCTTC(1/4) SbfI 72 CCTGCAGG CCTGCA^GG SfoI 61 GGCGCC GGC^GCC SmaI 870 CCCGGG CCC^GGG Sse8387I 72 CCTGCAGG CCTGCA^GG Tth111I 391 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 378 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 211 CTCGAG C^TCGAG XhoII 686 AGATCT [GA]^GATC[CT] XmaCI 870 CCCGGG C^CCGGG XmaI 870 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 11 CGCG CG^CG 51 CGCG AcyI 61 GGCGCC G[GA]^CG[CT]C 182 GGCGTC AvaI 211 CTCGAG C^[CT]CG[GA]G 870 CCCGGG BcnI 870 CCCGG CC^[GC]GG 871 CCGGG BglI 112 GCCCGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 718 GCCATGCTGGC BsaAI 336 CACGTA [CT]AC^GT[GA] 340 TACGTG BsaHI 61 GGCGCC G[GA]^CG[CT]C 182 GGCGTC BsoBI 211 CTCGAG C^[CT]CG[GA]G 870 CCCGGG BstUI 11 CGCG CG^CG 51 CGCG DpnII 486 GATC ^GATC 687 GATC DraIII 379 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 644 CACCCAGTG FauI 113 CCCGC CCCGC(4/6) 314 CCCGC HapII 475 CCGG C^CGG 871 CCGG Hin1I 61 GGCGCC G[GA]^CG[CT]C 182 GGCGTC HpaII 475 CCGG C^CGG 871 CCGG MboI 486 GATC ^GATC 687 GATC MslI 189 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 336 CACGTACGTG MspA1I 55 CAGCTG C[AC]G^C[GT]G 579 CAGCGG MspI 475 CCGG C^CGG 871 CCGG MvnI 11 CGCG CG^CG 51 CGCG NciI 870 CCCGG CC^[GC]GG 871 CCGGG NdeII 486 GATC ^GATC 687 GATC PleI 391 GACTC GAGTC(4/5) 490 GACTC PstI 73 CTGCAG CTGCA^G 157 CTGCAG Sau3AI 486 GATC ^GATC 687 GATC SexAI 380 ACCTGGT A^CC[AT]GGT 668 ACCTGGT SfaNI 347 GCATC GCATC(5/9) 374 GCATC SfcI 73 CTGCAG C^T[GA][CT]AG 157 CTGCAG TfiI 465 GAATC G^A[AT]TC 615 GATTC XcmI 188 CCATTGCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 371 CCAGCATCCACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI AlwNI BanI BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeII HaeIII HgaI HhaI HinP1I HinfI HphI ItaI Ksp632I MaeII MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SmlI TaqI TseI Tsp45I TspRI TthHB8I