################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BamHI BanI BanII BbeI BbrPI BbvCI BclI BcnI BfrI BglI BglII BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI Bsp1286I Bsp1407I BspDI BspHI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HphI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 785 TCCGGA T^CCGGA AflIII 459 ACATGT A^C[GA][CT]GT AocI 614 CCTCAGG CC^T[ACGT]AGG AvaI 1673 CTCGAG C^[CT]CG[GA]G AvaII 47 GGTCC G^G[AT]CC AvrII 409 CCTAGG C^CTAGG BalI 1131 TGGCCA TGG^CCA BbsI 1183 GAAGAC GAAGAC(2/6) BcgI 34 GCACCCCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1324 GGATAC GTATCC(6/5) BlnI 409 CCTAGG C^CTAGG BpuAI 1183 GAAGAC GAAGAC(2/6) BsaWI 785 TCCGGA [AT]^CCGG[AT] BseAI 785 TCCGGA T^CCGGA BsgI 32 CTGCAC GTGCAG(16/14) BsmBI 771 GAGACG CGTCTC(1/5) BsmFI 1210 GGGAC GGGAC(10/14) BsoBI 1673 CTCGAG C^[CT]CG[GA]G BspEI 785 TCCGGA T^CCGGA BspLU11I 459 ACATGT A^CATGT BsrBI 1231 GAGCGG CCGCTC(-3/-3) BstAPI 22 GCAGTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 962 CCATTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 614 CCTCAGG CC^T[ACGT]AGG DraI 243 TTTAAA TTT^AAA EaeI 1131 TGGCCA [CT]^GGCC[GA] Eco81I 614 CCTCAGG CC^T[ACGT]AGG EcoRI 324 GAATTC G^AATTC HapII 786 CCGG C^CGG HincII 1390 GTTAAC GT[CT]^[GA]AC HindII 1390 GTTAAC GT[CT]^[GA]AC HpaI 1390 GTTAAC GTT^AAC HpaII 786 CCGG C^CGG MaeII 1716 ACGT A^CGT MaeIII 1337 GTCAC ^GT[ACGT]AC MluNI 1131 TGGCCA TGG^CCA MroI 785 TCCGGA T^CCGGA MscI 1131 TGGCCA TGG^CCA MspA1I 1157 CAGCTG C[AC]G^C[GT]G MspI 786 CCGG C^CGG PaeR7I 1673 CTCGAG C^TCGAG PleI 763 GACTC GAGTC(4/5) PstI 146 CTGCAG CTGCA^G PvuII 1157 CAGCTG CAG^CTG ScaI 1196 AGTACT AGT^ACT SspI 818 AATATT AAT^ATT Tsp45I 1337 GTCAC ^GT[GC]AC XhoI 1673 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BbvI 31 GCTGC GCAGC(8/12) 1464 GCTGC BsiYI 37 CCCCTATCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 38 CCCTATCGGGG BslI 37 CCCCTATCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 38 CCCTATCGGGG BsrDI 137 GCAATG GCAATG(2/0) 871 GCAATG BssKI 748 CCTGG ^CC[ACGT]GG 1116 CCTGG BstNI 748 CCTGG CC^[AT]GG 1116 CCTGG DsaI 211 CCATGG C^C[GA][CT]GG 1736 CCATGG EcoRII 748 CCTGG ^CC[AT]GG 1116 CCTGG FauI 1601 CCCGC CCCGC(4/6) 1233 GCGGG MvaI 748 CCTGG CC^[AT]GG 1116 CCTGG NcoI 211 CCATGG C^CATGG 1736 CCATGG ScrFI 748 CCTGG CC^[ACGT]GG 1116 CCTGG SfcI 146 CTGCAG C^T[GA][CT]AG 1027 CTACAG SmlI 1200 CTCAAG C^T[CT][GA]AG 1673 CTCGAG TseI 31 GCTGC G^C[AT]GC 1464 GCTGC XcmI 557 CCAAAGTTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 646 CCAAAACACCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BfaI BpmI BsaI BsaJI BseRI BsmAI BsrI BstF5I BstYI Cac8I Cfr13I DdeI DpnII EarI EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MboI MboII MflI MnlI MseI MslI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I SfaNI StyI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I XhoII