(Circular) (Six-base) MAPSORT of: pFN21AE5607.seq Check: 7756 from: 1 to: 6463 pFN21AE5607 6463 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4273 278 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3806 1937 Size: 1869 4594 Acc65I G'GTAC_C Cuts at: 3784 3784 Size: 6463 AclI AA'CG_TT Cuts at: 1563 4590 4963 1563 Size: 3027 373 3063 Fragments arranged by size: 3063 3027 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2193 3042 3207 3711 4526 5910 6178 1632 Size: 561 849 165 504 815 1384 268 1917 Fragments arranged by size: 1917 1384 849 815 561 504 268 165 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6266 AflIII A'CryG_T Cuts at: 1203 2464 5362 6339 1203 Size: 1261 2898 977 1327 Fragments arranged by size: 2898 1327 1261 977 AgeI A'CCGG_T Cuts at: 5294 5294 Size: 6463 AhdI GACnn_n'nnGTC Cuts at: 5192 5192 Size: 6463 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6463 AlwNI CAG_nnn'CTG Cuts at: 4206 5778 4206 Size: 1572 4891 ApaLI G'TGCA_C Cuts at: 4520 5676 4520 Size: 1156 5307 ApoI r'AATT_y Cuts at: 1493 2329 2432 2680 2761 2833 2845 3772 3998 Size: 836 103 248 81 72 12 927 226 Cuts at: 3998 4150 6259 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 927 836 248 226 152 103 81 72 12 AseI AT'TA_AT Cuts at: 160 1032 5017 160 Size: 872 3985 1606 Fragments arranged by size: 3985 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 AvaI C'yCGr_G Cuts at: 1870 1943 3678 3788 1870 Size: 73 1735 110 4545 Fragments arranged by size: 4545 1735 110 73 AvrII C'CTAG_G Cuts at: 2414 2414 Size: 6463 BamHI G'GATC_C Cuts at: 3793 4160 3793 Size: 367 6096 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3784 5239 618 Size: 325 217 627 1997 1455 1842 Fragments arranged by size: 1997 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3782 1391 Size: 2391 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3195 928 Size: 971 1296 4196 Fragments arranged by size: 4196 1296 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2028 2062 4654 4688 1903 Size: 34 91 34 2592 34 3678 Fragments arranged by size: 3678 2592 91 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3323 4355 5565 3323 Size: 1032 1210 4221 Fragments arranged by size: 4221 1210 1032 BclI T'GATC_A Cuts at: 1548 1548 Size: 6463 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5074 136 Size: 107 122 71 631 605 3402 1525 Fragments arranged by size: 3402 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6458 6458 Size: 6463 BlpI GC'TnA_GC Cuts at: 3880 3880 Size: 6463 Bme1580I G_kGCm'C Cuts at: 1790 4524 5680 1790 Size: 2734 1156 2573 Fragments arranged by size: 2734 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6463 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4194 5152 Size: 416 53 83 399 24 2021 756 958 Cuts at: 5152 442 Size: 1753 Fragments arranged by size: 2021 1753 958 756 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2122 2319 2439 2955 3144 3393 5123 1239 Size: 883 197 120 516 189 249 1730 2579 Fragments arranged by size: 2579 1730 883 516 249 197 189 120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3190 4588 5453 5751 3190 Size: 1398 865 298 3902 Fragments arranged by size: 3902 1398 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2254 2404 3620 5126 882 Size: 617 755 150 1216 1506 2219 Fragments arranged by size: 2219 1506 1216 755 617 150 BsaAI yAC'GTr Cuts at: 493 1204 4254 493 Size: 711 3050 2702 Fragments arranged by size: 3050 2702 711 BsaBI GATnn'nnATC Cuts at: 6457 6457 Size: 6463 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4270 4652 5287 275 Size: 53 83 186 936 2737 382 635 1451 Fragments arranged by size: 2737 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2790 4896 5294 5568 5715 6126 2790 Size: 2106 398 274 147 411 3127 Fragments arranged by size: 3127 2106 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6238 Fragments arranged by size: 6238 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3278 3419 3614 1186 Size: 2092 141 195 4035 Fragments arranged by size: 4035 2092 195 141 BseYI C'CCAG_C Cuts at: 3380 5666 6162 3380 Size: 2286 496 3681 Fragments arranged by size: 3681 2286 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2022 1163 Size: 573 286 5604 Fragments arranged by size: 5604 573 286 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3913 4674 5702 1540 Size: 275 186 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4524 4609 5680 4524 Size: 85 1071 5307 Fragments arranged by size: 5307 1071 85 BsmI GAATG_Cn' Cuts at: 3974 4067 3974 Size: 93 6370 BsmBI CGTCTCn'nnnn_ Cuts at: 2770 2770 Size: 6463 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3782 4524 4609 5680 1391 Size: 399 1992 742 85 1071 2174 Fragments arranged by size: 2174 1992 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2790 6126 2790 Size: 3336 3127 BspHI T'CATG_A Cuts at: 4352 6082 4352 Size: 1730 4733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3818 844 Size: 2974 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3238 4350 1394 Size: 429 1415 1112 3507 Fragments arranged by size: 3507 1415 1112 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2149 2883 4958 5132 65 Size: 1183 901 734 2075 174 1396 Fragments arranged by size: 2075 1396 1183 901 734 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5107 5294 1700 Size: 110 3297 187 2869 Fragments arranged by size: 3297 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6463 BssSI C'ACGA_G Cuts at: 4523 5535 4523 Size: 1012 5451 BstAPI GCAn_nnn'nTGC Cuts at: 794 2033 794 Size: 1239 5224 BstBI TT'CG_AA Cuts at: 5267 5267 Size: 6463 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6463 BstXI CCAn_nnnn'nTGG Cuts at: 2974 2974 Size: 6463 BstYI r'GATC_y Cuts at: 1972 2839 3017 3040 3276 3793 4160 4545 4562 Size: 867 178 23 236 517 367 385 17 Cuts at: 4562 6014 6100 6112 6458 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 867 517 385 367 346 236 178 86 23 17 12 Bsu36I CC'TnA_GG Cuts at: 2620 2620 Size: 6463 BtgI C'CryG_G Cuts at: 513 1065 2216 3741 513 Size: 552 1151 1525 3235 Fragments arranged by size: 3235 1525 1151 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5724 Fragments arranged by size: 5724 683 56 BtsI GCAGTG_nn' Cuts at: 3979 4773 4793 3979 Size: 794 20 5649 Fragments arranged by size: 5649 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6428 DraI TTT'AAA Cuts at: 2250 3767 2250 Size: 1517 4946 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5470 809 Size: 507 4154 1802 Fragments arranged by size: 4154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3136 3910 4799 Size: 54 1425 50 275 18 1306 774 889 Cuts at: 4799 6428 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1306 889 774 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3910 1537 Size: 275 2098 4090 Fragments arranged by size: 4090 2098 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2495 2547 2664 3687 4393 1353 Size: 1142 52 117 1023 706 3423 Fragments arranged by size: 3423 1142 1023 706 117 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5050 5424 5570 387 Size: 1064 138 3461 374 146 1280 Fragments arranged by size: 3461 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2193 2319 2439 2955 3042 3144 Size: 393 490 71 126 120 516 87 102 Cuts at: 3144 3207 3393 3711 4526 5123 5910 6178 1239 Size: 63 186 318 815 597 787 268 1524 Fragments arranged by size: 1524 815 787 597 516 490 393 318 268 186 126 120 102 87 71 63 EcoNI CCTnn'n_nnAGG Cuts at: 3758 3758 Size: 6463 EcoRI G'AATT_C Cuts at: 2329 3772 4150 2329 Size: 1443 378 4642 Fragments arranged by size: 4642 1443 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2262 2262 Size: 6463 FspI TGC'GCA Cuts at: 4969 4969 Size: 6463 HaeII r_GCGC'y Cuts at: 1678 5610 1678 Size: 3932 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2872 2904 3002 3034 3268 Size: 32 135 32 1300 32 98 32 234 Cuts at: 3268 3300 5104 5136 5178 5210 1373 Size: 32 1804 32 42 32 2626 Fragments arranged by size: 2626 1804 1300 234 135 98 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3397 3807 4053 669 Size: 988 281 1459 410 246 3079 Fragments arranged by size: 3079 1459 988 410 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6463 HpaI GTT'AAC Cuts at: 3397 4053 3397 Size: 656 5807 KpnI G_GTAC'C Cuts at: 3788 3788 Size: 6463 MfeI C'AATT_G Cuts at: 4062 4062 Size: 6463 MluI A'CGCG_T Cuts at: 6339 6339 Size: 6463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2404 5577 5761 1305 Size: 1099 3173 184 2007 Fragments arranged by size: 3173 2007 1099 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3138 10 Size: 54 1425 343 1306 3335 Fragments arranged by size: 3335 1425 1306 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2706 2972 3150 4421 4780 4939 518 Size: 648 1540 266 178 1271 359 159 2042 Fragments arranged by size: 2042 1540 1271 648 359 266 178 159 MspA1I CmG'CkG Cuts at: 1606 3164 3879 4556 5704 5949 1606 Size: 1558 715 677 1148 245 2120 Fragments arranged by size: 2120 1558 1148 715 677 245 NcoI C'CATG_G Cuts at: 513 1065 2216 3741 513 Size: 552 1151 1525 3235 Fragments arranged by size: 3235 1525 1151 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6463 NotI GC'GGCC_GC Cuts at: 3910 3910 Size: 6463 NspI r_CATG'y Cuts at: 1132 1229 2364 2468 2752 3670 3821 5366 1132 Size: 97 1135 104 284 918 151 1545 2229 Fragments arranged by size: 2229 1545 1135 918 284 151 104 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6463 PciI A'CATG_T Cuts at: 2464 5362 2464 Size: 2898 3565 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6463 PfoI T'CCnGG_A Cuts at: 3120 3120 Size: 6463 PmeI GTTT'AAAC Cuts at: 3767 3767 Size: 6463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4532 4564 6045 6077 4532 Size: 32 1481 32 4918 Fragments arranged by size: 4918 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6463 PsiI TTA'TAA Cuts at: 3416 4033 3416 Size: 617 5846 PstI C_TGCA'G Cuts at: 830 2155 3815 830 Size: 1325 1660 3478 Fragments arranged by size: 3478 1660 1325 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6463 PvuII CAG'CTG Cuts at: 3164 3164 Size: 6463 SalI G'TCGA_C Cuts at: 1936 3805 1936 Size: 1869 4594 SbfI CC_TGCA'GG Cuts at: 3815 3815 Size: 6463 ScaI AGT'ACT Cuts at: 3203 4711 6423 3203 Size: 1508 1712 3243 Fragments arranged by size: 3243 1712 1508 SfcI C'TryA_G Cuts at: 826 1045 2151 3032 3811 4946 5627 5818 826 Size: 219 1106 881 779 1135 681 191 1471 Fragments arranged by size: 1471 1135 1106 881 779 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6463 SmaI CCC'GGG Cuts at: 1872 3790 1872 Size: 1918 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3205 3678 4567 5468 5730 820 Size: 197 926 1262 473 889 901 262 1553 Fragments arranged by size: 1553 1262 926 901 889 473 262 197 SnaBI TAC'GTA Cuts at: 493 4254 493 Size: 3761 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6463 SphI G_CATG'C Cuts at: 1132 3821 1132 Size: 2689 3774 SspI AAT'ATT Cuts at: 5 52 2825 4387 5 Size: 47 2773 1562 2081 Fragments arranged by size: 2773 2081 1562 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6463 StyI C'CwwG_G Cuts at: 513 1065 2216 2414 3741 3754 3902 513 Size: 552 1151 198 1327 13 148 3074 Fragments arranged by size: 3074 1327 1151 552 198 148 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4490 4507 4660 4845 5290 325 Size: 1255 2910 17 153 185 445 1498 Fragments arranged by size: 2910 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3201 3368 4709 Size: 274 80 33 51 1443 1224 167 1341 Cuts at: 4709 6421 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1341 1224 274 167 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 3009 3041 6423 6455 2616 Size: 32 361 32 3382 32 2624 Fragments arranged by size: 3382 2624 361 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6463 XbaI T'CTAG_A Cuts at: 3799 3799 Size: 6463 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2569 2658 2569 Size: 89 6374 XhoI C'TCGA_G Cuts at: 1943 3678 1943 Size: 1735 4728 XmaI C'CCGG_G Cuts at: 1870 3788 1870 Size: 1918 4545 XmnI GAAnn'nnTTC Cuts at: 4592 4592 Size: 6463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4271 276 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI