################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AccIII AclI AflIII AgeI AhdI AscI AseI AsnI Asp700I AspEI AspI AvrII BbeI BbrPI BcgI BciVI BclI BlnI BsaAI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI MfeI MluI MroI MslI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI RcaI RsrII SalI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1113 GACGTC GACGT^C Acc65I 1511 GGTACC G^GTACC AccI 868 GTAGAC GT^[AC][GT]AC AcsI 409 AAATTT [GA]^AATT[CT] AflII 910 CTTAAG C^TTAAG Alw44I 1387 GTGCAC G^TGCAC AocI 1417 CCTGAGG CC^T[ACGT]AGG ApaLI 1387 GTGCAC G^TGCAC ApoI 409 AAATTT [GA]^AATT[CT] Asp718I 1511 GGTACC G^GTACC AviII 575 TGCGCA TGC^GCA BamHI 1487 GGATCC G^GATCC BbsI 1576 GTCTTC GAAGAC(2/6) BbvCI 415 GCTGAGG CCTCAGC(-5/-2) BfrI 910 CTTAAG C^TTAAG BglII 1472 AGATCT A^GATCT BpuAI 1576 GTCTTC GAAGAC(2/6) BsaBI 381 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1138 GTGCAG GTGCAG(16/14) BsmI 753 GCATTC GAATGC(1/-1) BsrDI 679 GCAATG GCAATG(2/0) BsrFI 1334 GCCGGC [GA]^CCGG[CT] BstAPI 282 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 630 CCAGGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1417 CCTGAGG CC^T[ACGT]AGG Cfr10I 1334 GCCGGC [GA]^CCGG[CT] DraIII 670 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1209 GACCTTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1417 CCTGAGG CC^T[ACGT]AGG EcoNI 624 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 575 TGCGCA TGC^GCA HgaI 1758 GCGTC GACGC(5/10) KpnI 1511 GGTACC GGTAC^C KspI 1006 CCGCGG CCGC^GG MamI 381 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 911 TTAA T^TAA NaeI 1334 GCCGGC GCC^GGC NgoMIV 1334 GCCGGC G^CCGGC PflMI 1173 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 1006 CCGCGG CCGC^GG SapI 277 GAAGAGC GCTCTTC(1/4) SexAI 671 ACCTGGT A^CC[AT]GGT SfcI 1317 CTACAG C^T[GA][CT]AG SfiI 1414 GGCCCTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 647 CCCGGG CCC^GGG SmlI 910 CTTAAG C^T[CT][GA]AG Tru9I 911 TTAA T^TAA Tsp509I 410 AATT ^AATT Van91I 1173 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 647 CCCGGG C^CCGGG XmaI 647 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 1113 GACGTC G[GA]^CG[CT]C 1757 GGCGTC Aor51HI 42 AGCGCT AGC^GCT 1367 AGCGCT BfaI 329 CTAG C^TAG 1570 CTAG BglI 700 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1415 GCCCTGAGGGC BlpI 3 GCTGAGC GC^T[ACGT]AGC 1502 GCTCAGC Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC 1502 GCTCAGC BsaHI 1113 GACGTC G[GA]^CG[CT]C 1757 GGCGTC BsiEI 134 CGATCG CG[GA][CT]^CG 386 CGATCG BsmBI 130 GAGACG CGTCTC(1/5) 1111 GAGACG CelII 3 GCTGAGC GC^T[ACGT]AGC 1502 GCTCAGC EarI 253 CTCTTC CTCTTC(1/4) 277 GAAGAG Eco47III 42 AGCGCT AGC^GCT 1367 AGCGCT HaeII 42 AGCGCT [GA]GCGC^[CT] 1367 AGCGCT Hin1I 1113 GACGTC G[GA]^CG[CT]C 1757 GGCGTC Ksp632I 253 CTCTTC CTCTTC(1/4) 277 GAAGAG MaeI 329 CTAG C^TAG 1570 CTAG MaeII 1114 ACGT A^CGT 1693 ACGT NcoI 1478 CCATGG C^CATGG 1604 CCATGG NspI 905 ACATGC [GA]CATG^[CT] 1391 ACATGC PvuI 134 CGATCG CGAT^CG 386 CGATCG PvuII 96 CAGCTG CAG^CTG 1177 CAGCTG SacI 1011 GAGCTC GAGCT^C 1282 GAGCTC Tsp45I 215 GTGAC ^GT[GC]AC 724 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I XhoII