(Circular) (Six-base) MAPSORT of: pFN21AE5703.seq Check: 8184 from: 1 to: 6646 pFN21AE5703 6646 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3122 4456 278 Size: 53 83 186 936 1586 1334 2468 Fragments arranged by size: 2468 1586 1334 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2874 3989 1937 Size: 937 1115 4594 Fragments arranged by size: 4594 1115 937 Acc65I G'GTAC_C Cuts at: 3516 3967 3516 Size: 451 6195 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2914 3290 3353 3624 3767 3800 3845 4709 Size: 1282 376 63 271 143 33 45 864 Cuts at: 4709 6093 6361 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1282 864 376 271 268 143 63 45 33 AfeI AGC'GCT Cuts at: 2049 3374 2049 Size: 1325 5321 AflII C'TTAA_G Cuts at: 820 1017 2915 820 Size: 197 1898 4551 Fragments arranged by size: 4551 1898 197 AflIII A'CryG_T Cuts at: 1203 5545 6522 1203 Size: 4342 977 1327 Fragments arranged by size: 4342 1327 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 5375 5375 Size: 6646 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6646 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6646 AlwNI CAG_nnn'CTG Cuts at: 2096 2293 2864 3241 3823 4389 5961 2096 Size: 197 571 377 582 566 1572 2781 Fragments arranged by size: 2781 1572 582 571 566 377 197 ApaI G_GGCC'C Cuts at: 2240 3422 3431 2240 Size: 1182 9 5455 Fragments arranged by size: 5455 1182 9 ApaLI G'TGCA_C Cuts at: 3392 4703 5859 3392 Size: 1311 1156 4179 Fragments arranged by size: 4179 1311 1156 ApoI r'AATT_y Cuts at: 1493 2414 3955 4181 4333 6442 1493 Size: 921 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 921 226 152 AseI AT'TA_AT Cuts at: 160 1032 5200 160 Size: 872 4168 1606 Fragments arranged by size: 4168 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 2233 2309 2474 2502 2652 3656 3844 Size: 73 290 76 165 28 150 1004 188 Cuts at: 3844 3971 1870 Size: 127 4545 Fragments arranged by size: 4545 1004 290 188 165 150 127 76 73 28 BamHI G'GATC_C Cuts at: 3492 3976 4343 3492 Size: 484 367 5795 Fragments arranged by size: 5795 484 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2317 2832 3516 3967 5422 Size: 325 217 627 530 515 684 451 1455 Cuts at: 5422 618 Size: 1842 Fragments arranged by size: 1842 1455 684 627 530 515 451 325 217 BanII G_rGCy'C Cuts at: 1391 2240 3020 3291 3422 3431 3509 3965 1391 Size: 849 780 271 131 9 78 456 4072 Fragments arranged by size: 4072 849 780 456 271 131 78 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3574 928 Size: 971 1675 4000 Fragments arranged by size: 4000 1675 971 BbvCI CC'TCA_GC Cuts at: 2421 2421 Size: 6646 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4837 4871 1903 Size: 34 50 34 2816 34 3678 Fragments arranged by size: 3678 2816 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4538 5748 4538 Size: 1210 5436 BclI T'GATC_A Cuts at: 1548 1548 Size: 6646 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2711 3426 5257 Size: 107 122 71 631 605 1039 715 1831 Cuts at: 5257 136 Size: 1525 Fragments arranged by size: 1831 1525 1039 715 631 605 122 107 71 BglII A'GATC_T Cuts at: 3477 6641 3477 Size: 3164 3482 BlpI GC'TnA_GC Cuts at: 2009 3508 4063 2009 Size: 1499 555 4592 Fragments arranged by size: 4592 1499 555 Bme1580I G_kGCm'C Cuts at: 1790 2240 2320 2599 2835 3396 3422 3431 4707 Size: 450 80 279 236 561 26 9 1276 Cuts at: 4707 5863 1790 Size: 1156 2573 Fragments arranged by size: 2573 1276 1156 561 450 279 236 80 26 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6646 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2555 2702 3801 Size: 416 53 83 399 24 1138 147 1099 Cuts at: 3801 4377 5335 442 Size: 576 958 1753 Fragments arranged by size: 1753 1138 1099 958 576 416 399 147 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6646 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2066 2066 Size: 6646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2511 3077 3121 3725 5306 1239 Size: 1272 566 44 604 1581 2579 Fragments arranged by size: 2579 1581 1272 604 566 44 Bpu10I CC'TnA_GC Cuts at: 2421 3290 3300 2421 Size: 869 10 5767 Fragments arranged by size: 5767 869 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4771 5636 5934 4771 Size: 865 298 5483 Fragments arranged by size: 5483 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2499 3539 3645 3929 5309 882 Size: 617 1000 1040 106 284 1380 2219 Fragments arranged by size: 2219 1380 1040 1000 617 284 106 BsaAI yAC'GTr Cuts at: 493 1204 4437 493 Size: 711 3233 2702 Fragments arranged by size: 3233 2702 711 BsaBI GATnn'nnATC Cuts at: 2390 6640 2390 Size: 4250 2396 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3119 3763 4453 4835 Size: 53 83 186 936 1586 644 690 382 Cuts at: 4835 5470 275 Size: 635 1451 Fragments arranged by size: 1586 1451 936 690 644 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5079 5477 5751 5898 6309 5079 Size: 398 274 147 411 5416 Fragments arranged by size: 5416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2203 2233 1178 Size: 30 165 30 800 30 5591 Fragments arranged by size: 5591 800 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2438 2462 3098 3176 1186 Size: 973 279 24 636 78 4656 Fragments arranged by size: 4656 973 636 279 78 24 BseYI C'CCAG_C Cuts at: 2021 2207 2369 2701 3026 3184 3255 3646 5849 Size: 186 162 332 325 158 71 391 2203 Cuts at: 5849 6345 2021 Size: 496 2322 Fragments arranged by size: 2322 2203 496 391 332 325 186 162 158 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3164 1163 Size: 573 1428 4645 Fragments arranged by size: 4645 1428 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2142 2394 4096 4857 5885 1540 Size: 275 186 141 252 1702 761 1028 2301 Fragments arranged by size: 2301 1702 1028 761 275 252 186 141 BsiHKAI G_wGCw'C Cuts at: 3020 3291 3396 4707 4792 5863 3020 Size: 271 105 1311 85 1071 3803 Fragments arranged by size: 3803 1311 1071 271 105 85 BsmI GAATG_Cn' Cuts at: 2758 4157 4250 2758 Size: 1399 93 5154 Fragments arranged by size: 5154 1399 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2129 3110 2129 Size: 981 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2320 2599 2835 3020 3291 3396 Size: 399 450 80 279 236 185 271 105 Cuts at: 3396 3422 3431 3509 3965 4707 4792 5863 1391 Size: 26 9 78 456 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 456 450 399 279 271 236 185 105 85 80 78 26 9 BspEI T'CCGG_A Cuts at: 6309 6309 Size: 6646 BspHI T'CATG_A Cuts at: 4535 6265 4535 Size: 1730 4916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3099 3122 3258 4001 844 Size: 2255 23 136 743 3489 Fragments arranged by size: 3489 2255 743 136 23 BsrBI CCG'CTC Cuts at: 1394 1823 2013 2018 3860 4533 1394 Size: 429 190 5 1842 673 3507 Fragments arranged by size: 3507 1842 673 429 190 5 BsrDI GCAATG_nn' Cuts at: 65 1248 2691 5141 5315 65 Size: 1183 1443 2450 174 1396 Fragments arranged by size: 2450 1443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3339 5290 5477 1700 Size: 110 1529 1951 187 2869 Fragments arranged by size: 2869 1951 1529 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 4706 5718 4706 Size: 1012 5634 BstAPI GCAn_nnn'nTGC Cuts at: 794 2293 794 Size: 1499 5147 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6646 BstXI CCAn_nnnn'nTGG Cuts at: 2642 2642 Size: 6646 BstYI r'GATC_y Cuts at: 1972 3147 3477 3492 3708 3976 4343 4728 4745 Size: 1175 330 15 216 268 367 385 17 Cuts at: 4745 6197 6283 6295 6641 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1175 385 367 346 330 268 216 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 3423 3423 Size: 6646 BtgI C'CryG_G Cuts at: 513 1065 2572 3011 3483 3609 3907 513 Size: 552 1507 439 472 126 298 3252 Fragments arranged by size: 3252 1507 552 472 439 298 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2787 487 Size: 683 56 1561 4346 Fragments arranged by size: 4346 1561 683 56 BtsI GCAGTG_nn' Cuts at: 2300 2941 3055 3379 4162 4956 4976 2300 Size: 641 114 324 783 794 20 3970 Fragments arranged by size: 3970 794 783 641 324 114 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2914 2949 529 Size: 35 2350 35 4226 Fragments arranged by size: 4226 2350 35 35 DraI TTT'AAA Cuts at: 3950 3950 Size: 6646 DraIII CAC_nnn'GTG Cuts at: 2680 2680 Size: 6646 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3220 5653 809 Size: 507 1904 2433 1802 Fragments arranged by size: 2433 1904 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2511 2793 Size: 54 1425 50 275 18 531 150 282 Cuts at: 2793 2893 4093 4982 6611 8 Size: 100 1200 889 1629 43 Fragments arranged by size: 1629 1425 1200 889 531 282 275 150 100 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4093 1537 Size: 275 2281 4090 Fragments arranged by size: 4090 2281 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 2277 4576 1353 Size: 911 13 2299 3423 Fragments arranged by size: 3423 2299 911 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2055 2938 5233 5607 5753 387 Size: 1064 138 466 883 2295 374 146 1280 Fragments arranged by size: 2295 1280 1064 883 466 374 146 138 EcoICRI GAG'CTC Cuts at: 3018 3289 3018 Size: 271 6375 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2511 2914 3077 3121 3290 3353 3624 Size: 393 879 403 163 44 169 63 271 Cuts at: 3624 3725 3767 3800 3845 4709 5306 6093 6361 Size: 101 42 33 45 864 597 787 268 Cuts at: 6361 1239 Size: 1524 Fragments arranged by size: 1524 879 864 787 597 403 393 271 268 169 163 101 63 45 44 42 33 EcoNI CCTnn'n_nnAGG Cuts at: 2633 2633 Size: 6646 EcoO109I rG'GnC_Cy Cuts at: 2237 2567 3418 3419 3427 3592 2237 Size: 330 851 1 8 165 5291 Fragments arranged by size: 5291 851 330 165 8 1 EcoRI G'AATT_C Cuts at: 3955 4333 3955 Size: 378 6268 FspI TGC'GCA Cuts at: 2582 5152 2582 Size: 2570 4076 HaeII r_GCGC'y Cuts at: 1678 2051 3376 5793 1678 Size: 373 1325 2417 2531 Fragments arranged by size: 2531 2417 1325 373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2656 2688 5287 5319 5361 Size: 32 135 32 1084 32 2599 32 42 Cuts at: 5361 5393 1373 Size: 32 2626 Fragments arranged by size: 2626 2599 1084 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3990 4236 669 Size: 988 281 2052 246 3079 Fragments arranged by size: 3079 2052 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KpnI G_GTAC'C Cuts at: 3520 3971 3520 Size: 451 6195 MfeI C'AATT_G Cuts at: 4245 4245 Size: 6646 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5760 5944 1305 Size: 4455 184 2007 Fragments arranged by size: 4455 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 2513 2795 2895 10 Size: 54 1425 343 531 150 282 100 3761 Fragments arranged by size: 3761 1425 531 343 282 150 100 54 MslI CAynn'nnrTG Cuts at: 518 1166 4604 4963 5122 518 Size: 648 3438 359 159 2042 Fragments arranged by size: 3438 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2103 2176 2293 2696 3013 3184 3238 4062 Size: 497 73 117 403 317 171 54 824 Cuts at: 4062 4739 5887 6132 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 677 497 403 317 245 171 117 73 54 NaeI GCC'GGC Cuts at: 3341 3341 Size: 6646 NcoI C'CATG_G Cuts at: 513 1065 3483 3609 513 Size: 552 2418 126 3550 Fragments arranged by size: 3550 2418 552 126 NdeI CA'TA_TG Cuts at: 387 387 Size: 6646 NgoMIV G'CCGG_C Cuts at: 3339 3339 Size: 6646 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6646 NotI GC'GGCC_GC Cuts at: 4093 4093 Size: 6646 NspI r_CATG'y Cuts at: 1132 1229 2914 3400 4004 5549 1132 Size: 97 1685 486 604 1545 2229 Fragments arranged by size: 2229 1685 1545 604 486 97 PasI CC'CwG_GG Cuts at: 1348 3261 3409 1348 Size: 1913 148 4585 Fragments arranged by size: 4585 1913 148 PciI A'CATG_T Cuts at: 5545 5545 Size: 6646 PflMI CCAn_nnn'nTGG Cuts at: 1108 3184 1108 Size: 2076 4570 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4715 4747 6228 6260 4715 Size: 32 1481 32 5101 Fragments arranged by size: 5101 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6646 PsiI TTA'TAA Cuts at: 4216 4216 Size: 6646 PspOMI G'GGCC_C Cuts at: 2236 3418 3427 2236 Size: 1182 9 5455 Fragments arranged by size: 5455 1182 9 PstI C_TGCA'G Cuts at: 830 3998 830 Size: 3168 3478 PvuI CG_AT'CG Cuts at: 2001 2142 2394 2001 Size: 141 252 6253 Fragments arranged by size: 6253 252 141 PvuII CAG'CTG Cuts at: 2103 3184 2103 Size: 1081 5565 SacI G_AGCT'C Cuts at: 3020 3291 3020 Size: 271 6375 SacII CC_GC'GG Cuts at: 3014 3014 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SapI GCTCTTCn'nnn_ Cuts at: 2277 2277 Size: 6646 SbfI CC_TGCA'GG Cuts at: 3998 3998 Size: 6646 ScaI AGT'ACT Cuts at: 4894 6606 4894 Size: 1712 4934 SexAI A'CCwGG_T Cuts at: 2676 2676 Size: 6646 SfcI C'TryA_G Cuts at: 826 1045 3322 3994 5129 5810 6001 826 Size: 219 2277 672 1135 681 191 1471 Fragments arranged by size: 2277 1471 1135 681 672 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3426 3426 Size: 6646 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 2654 3973 1872 Size: 782 1319 4545 Fragments arranged by size: 4545 1319 782 SmlI C'TyrA_G Cuts at: 820 1017 1943 2915 4750 5651 5913 820 Size: 197 926 972 1835 901 262 1553 Fragments arranged by size: 1835 1553 972 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 4004 1132 Size: 2872 3774 SspI AAT'ATT Cuts at: 5 52 4570 5 Size: 47 4518 2081 Fragments arranged by size: 4518 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6646 StyI C'CwwG_G Cuts at: 513 1065 2439 3483 3609 4085 513 Size: 552 1374 1044 126 476 3074 Fragments arranged by size: 3074 1374 1044 552 476 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 4673 4690 4843 5028 5473 325 Size: 1255 1821 1272 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1272 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3531 4892 6604 Size: 274 80 33 51 1443 1554 1361 1712 Cuts at: 6604 96 Size: 138 Fragments arranged by size: 1712 1554 1443 1361 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2203 2235 6606 6638 2203 Size: 32 4371 32 2211 Fragments arranged by size: 4371 2211 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6646 XbaI T'CTAG_A Cuts at: 3982 3982 Size: 6646 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6646 XmaI C'CCGG_G Cuts at: 1870 2652 3971 1870 Size: 782 1319 4545 Fragments arranged by size: 4545 1319 782 XmnI GAAnn'nnTTC Cuts at: 4775 4775 Size: 6646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3120 4454 276 Size: 53 83 186 936 1586 1334 2468 Fragments arranged by size: 2468 1586 1334 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SfoI SrfI SwaI XcmI