(Linear) (Six-base) MAPSORT of: KIEE5703.seq Check: 211 from: 1 to: 1941 KIEE5703.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AatII G_ACGT'C Cuts at: 0 1117 1941 Size: 1117 824 AccI GT'mk_AC Cuts at: 0 869 1941 Size: 869 1072 Acc65I G'GTAC_C Cuts at: 0 1511 1941 Size: 1511 430 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 909 1285 1348 1619 1762 1795 1840 1941 Size: 909 376 63 271 143 33 45 101 Fragments arranged by size: 909 376 271 143 101 63 45 33 AfeI AGC'GCT Cuts at: 0 44 1369 1941 Size: 44 1325 572 Fragments arranged by size: 1325 572 44 AflII C'TTAA_G Cuts at: 0 910 1941 Size: 910 1031 AlwNI CAG_nnn'CTG Cuts at: 0 91 288 859 1236 1818 1941 Size: 91 197 571 377 582 123 Fragments arranged by size: 582 571 377 197 123 91 ApaI G_GGCC'C Cuts at: 0 235 1417 1426 1941 Size: 235 1182 9 515 Fragments arranged by size: 1182 515 235 9 ApaLI G'TGCA_C Cuts at: 0 1387 1941 Size: 1387 554 ApoI r'AATT_y Cuts at: 0 409 1941 Size: 409 1532 AvaI C'yCGr_G Cuts at: 0 228 304 469 497 647 1651 1839 1941 Size: 228 76 165 28 150 1004 188 102 Fragments arranged by size: 1004 228 188 165 150 102 76 28 BamHI G'GATC_C Cuts at: 0 1487 1941 Size: 1487 454 BanI G'GyrC_C Cuts at: 0 312 827 1511 1941 Size: 312 515 684 430 Fragments arranged by size: 684 515 430 312 BanII G_rGCy'C Cuts at: 0 235 1015 1286 1417 1426 1504 1941 Size: 235 780 271 131 9 78 437 Fragments arranged by size: 780 437 271 235 131 78 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 1569 1941 Size: 1569 372 BbvCI CC'TCA_GC Cuts at: 0 416 1941 Size: 416 1525 BglI GCCn_nnn'nGGC Cuts at: 0 706 1421 1941 Size: 706 715 520 Fragments arranged by size: 715 706 520 BglII A'GATC_T Cuts at: 0 1472 1941 Size: 1472 469 BlpI GC'TnA_GC Cuts at: 0 4 1503 1941 Size: 4 1499 438 Fragments arranged by size: 1499 438 4 Bme1580I G_kGCm'C Cuts at: 0 235 315 594 830 1391 1417 1426 1941 Size: 235 80 279 236 561 26 9 515 Fragments arranged by size: 561 515 279 236 235 80 26 9 BmrI ACTGGGnnnn_n' Cuts at: 0 550 697 1796 1941 Size: 550 147 1099 145 Fragments arranged by size: 1099 550 147 145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 61 1941 Size: 61 1880 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 506 1072 1116 1720 1941 Size: 506 566 44 604 221 Fragments arranged by size: 604 566 506 221 44 Bpu10I CC'TnA_GC Cuts at: 0 416 1285 1295 1941 Size: 416 869 10 646 Fragments arranged by size: 869 646 416 10 BsaI GGTCTCn'nnnn_ Cuts at: 0 494 1534 1640 1924 1941 Size: 494 1040 106 284 17 Fragments arranged by size: 1040 494 284 106 17 BsaBI GATnn'nnATC Cuts at: 0 385 1941 Size: 385 1556 BsaHI Gr'CG_yC Cuts at: 0 1114 1758 1941 Size: 1114 644 183 Fragments arranged by size: 1114 644 183 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 198 228 1941 Size: 198 30 1713 Fragments arranged by size: 1713 198 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 154 433 457 1093 1171 1941 Size: 154 279 24 636 78 770 Fragments arranged by size: 770 636 279 154 78 24 BseYI C'CCAG_C Cuts at: 0 16 202 364 696 1021 1179 1250 1641 Size: 16 186 162 332 325 158 71 391 Cuts at: 1641 1941 Size: 300 Fragments arranged by size: 391 332 325 300 186 162 158 71 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1159 1941 Size: 1159 782 BsiEI CG_ry'CG Cuts at: 0 137 389 1941 Size: 137 252 1552 Fragments arranged by size: 1552 252 137 BsiHKAI G_wGCw'C Cuts at: 0 1015 1286 1391 1941 Size: 1015 271 105 550 Fragments arranged by size: 1015 550 271 105 BsmI GAATG_Cn' Cuts at: 0 753 1941 Size: 753 1188 BsmBI CGTCTCn'nnnn_ Cuts at: 0 124 1105 1941 Size: 124 981 836 Fragments arranged by size: 981 836 124 Bsp1286I G_dGCh'C Cuts at: 0 235 315 594 830 1015 1286 1391 1417 Size: 235 80 279 236 185 271 105 26 Cuts at: 1417 1426 1504 1941 Size: 9 78 437 Fragments arranged by size: 437 279 271 236 235 185 105 80 78 26 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1094 1117 1253 1941 Size: 1094 23 136 688 Fragments arranged by size: 1094 688 136 23 BsrBI CCG'CTC Cuts at: 0 8 13 1855 1941 Size: 8 5 1842 86 Fragments arranged by size: 1842 86 8 5 BsrDI GCAATG_nn' Cuts at: 0 686 1941 Size: 686 1255 BsrFI r'CCGG_y Cuts at: 0 1334 1941 Size: 1334 607 BstAPI GCAn_nnn'nTGC Cuts at: 0 288 1941 Size: 288 1653 BstXI CCAn_nnnn'nTGG Cuts at: 0 637 1941 Size: 637 1304 BstYI r'GATC_y Cuts at: 0 1142 1472 1487 1703 1941 Size: 1142 330 15 216 238 Fragments arranged by size: 1142 330 238 216 15 Bsu36I CC'TnA_GG Cuts at: 0 1418 1941 Size: 1418 523 BtgI C'CryG_G Cuts at: 0 567 1006 1478 1604 1902 1941 Size: 567 439 472 126 298 39 Fragments arranged by size: 567 472 439 298 126 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 782 1941 Size: 782 1159 BtsI GCAGTG_nn' Cuts at: 0 295 936 1050 1374 1941 Size: 295 641 114 324 567 Fragments arranged by size: 641 567 324 295 114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 909 944 1941 Size: 909 35 997 Fragments arranged by size: 997 909 35 DraIII CAC_nnn'GTG Cuts at: 0 675 1941 Size: 675 1266 DrdI GACnn_nn'nnGTC Cuts at: 0 1215 1941 Size: 1215 726 EaeI y'GGCC_r Cuts at: 0 356 506 788 888 1941 Size: 356 150 282 100 1053 Fragments arranged by size: 1053 356 282 150 100 EarI CTCTTCn'nnn_ Cuts at: 0 259 272 1941 Size: 259 13 1669 Fragments arranged by size: 1669 259 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 50 933 1941 Size: 50 883 1008 Fragments arranged by size: 1008 883 50 EcoICRI GAG'CTC Cuts at: 0 1013 1284 1941 Size: 1013 271 657 Fragments arranged by size: 1013 657 271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 506 909 1072 1116 1285 1348 1619 1720 Size: 506 403 163 44 169 63 271 101 Cuts at: 1720 1762 1795 1840 1941 Size: 42 33 45 101 Fragments arranged by size: 506 403 271 169 163 101 101 63 45 44 42 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 628 1941 Size: 628 1313 EcoO109I rG'GnC_Cy Cuts at: 0 232 562 1413 1414 1422 1587 1941 Size: 232 330 851 1 8 165 354 Fragments arranged by size: 851 354 330 232 165 8 1 FspI TGC'GCA Cuts at: 0 577 1941 Size: 577 1364 HaeII r_GCGC'y Cuts at: 0 46 1371 1941 Size: 46 1325 570 Fragments arranged by size: 1325 570 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 651 683 1941 Size: 651 32 1258 Fragments arranged by size: 1258 651 32 KpnI G_GTAC'C Cuts at: 0 1515 1941 Size: 1515 426 MscI TGG'CCA Cuts at: 0 358 508 790 890 1941 Size: 358 150 282 100 1051 Fragments arranged by size: 1051 358 282 150 100 MspA1I CmG'CkG Cuts at: 0 98 171 288 691 1008 1179 1233 1941 Size: 98 73 117 403 317 171 54 708 Fragments arranged by size: 708 403 317 171 117 98 73 54 NaeI GCC'GGC Cuts at: 0 1336 1941 Size: 1336 605 NcoI C'CATG_G Cuts at: 0 1478 1604 1941 Size: 1478 126 337 Fragments arranged by size: 1478 337 126 NgoMIV G'CCGG_C Cuts at: 0 1334 1941 Size: 1334 607 NspI r_CATG'y Cuts at: 0 909 1395 1941 Size: 909 486 546 Fragments arranged by size: 909 546 486 PasI CC'CwG_GG Cuts at: 0 1256 1404 1941 Size: 1256 148 537 Fragments arranged by size: 1256 537 148 PflMI CCAn_nnn'nTGG Cuts at: 0 1179 1941 Size: 1179 762 PspOMI G'GGCC_C Cuts at: 0 231 1413 1422 1941 Size: 231 1182 9 519 Fragments arranged by size: 1182 519 231 9 PvuI CG_AT'CG Cuts at: 0 137 389 1941 Size: 137 252 1552 Fragments arranged by size: 1552 252 137 PvuII CAG'CTG Cuts at: 0 98 1179 1941 Size: 98 1081 762 Fragments arranged by size: 1081 762 98 SacI G_AGCT'C Cuts at: 0 1015 1286 1941 Size: 1015 271 655 Fragments arranged by size: 1015 655 271 SacII CC_GC'GG Cuts at: 0 1009 1941 Size: 1009 932 SapI GCTCTTCn'nnn_ Cuts at: 0 272 1941 Size: 272 1669 SexAI A'CCwGG_T Cuts at: 0 671 1941 Size: 671 1270 SfcI C'TryA_G Cuts at: 0 1317 1941 Size: 1317 624 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1421 1941 Size: 1421 520 SmaI CCC'GGG Cuts at: 0 649 1941 Size: 649 1292 SmlI C'TyrA_G Cuts at: 0 910 1941 Size: 910 1031 StyI C'CwwG_G Cuts at: 0 434 1478 1604 1941 Size: 434 1044 126 337 Fragments arranged by size: 1044 434 337 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1396 1941 Size: 1396 545 TatI w'GTAC_w Cuts at: 0 1526 1941 Size: 1526 415 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 198 230 1941 Size: 198 32 1711 Fragments arranged by size: 1711 198 32 XmaI C'CCGG_G Cuts at: 0 647 1941 Size: 647 1294 ZraI GAC'GTC Cuts at: 0 1115 1941 Size: 1115 826 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflII AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI MscI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PspOMI PvuI PvuII SacI SacII SapI SexAI SfcI SfiI SmaI SmlI StyI TaqII TatI TstI XmaI ZraI Enzymes that do not cut: AarI AclI AflIII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BpuEI BsaAI BsaWI BsiWI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI DraI EagI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MslI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI RsrII SalI SanDI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI