################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccIII AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AviII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BpmI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HpaII KasI KspI MamI MflI MluI MroI MspA1I MspI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PstI PvuI PvuII RcaI RsrII SacI SacII SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 117 GGTACC G^GTACC AclI 211 AACGTT AA^CGTT AcyI 9 GACGCC G[GA]^CG[CT]C AflIII 882 ACATGT A^C[GA][CT]GT AlwI 318 GGATC GGATC(4/5) AseI 1900 ATTAAT AT^TAAT AsnI 1900 ATTAAT AT^TAAT Asp718I 117 GGTACC G^GTACC AspHI 675 GTGCTC G[AT]GC[AT]^C AvaI 1255 CCCGAG C^[CT]CG[GA]G AvaII 1276 GGACC G^G[AT]CC AvrII 1266 CCTAGG C^CTAGG BalI 488 TGGCCA TGG^CCA BanI 117 GGTACC G^G[CT][GA]CC BanII 1484 GAGCCC G[GA]GC[CT]^C BbvI 1842 GCAGC GCAGC(8/12) BlnI 1266 CCTAGG C^CTAGG BlpI 1837 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1837 GCTAAGC GC^T[ACGT]AGC BsaAI 410 TACGTA [CT]AC^GT[GA] BsaHI 9 GACGCC G[GA]^CG[CT]C BsiHKAI 675 GTGCTC G[AT]GC[AT]^C BsoBI 1255 CCCGAG C^[CT]CG[GA]G BspLU11I 882 ACATGT A^CATGT BspMI 1002 GCAGGT ACCTGC(4/8) BsrBI 16 CCGCTC CCGCTC(-3/-3) BsrI 1160 ACTGG ACTGG(1/-1) BssSI 1167 CTCGTG CACGAG(-5/-1) Cac8I 1584 GCTAGC GC[ACGT]^[ACGT]GC CelII 1837 GCTAAGC GC^T[ACGT]AGC CfoI 37 GCGC GCG^C Cfr13I 1276 GGACC G^G[ACGT]CC DraI 1543 TTTAAA TTT^AAA DraII 1275 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 488 TGGCCA [CT]^GGCC[GA] EcoNI 1702 CCTGTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1275 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 1393 GAATTC G^AATTC HhaI 37 GCGC GCG^C Hin1I 9 GACGCC G[GA]^CG[CT]C HinP1I 37 GCGC G^CGC HindIII 158 AAGCTT A^AGCTT HpaI 1311 GTTAAC GTT^AAC KpnI 117 GGTACC GGTAC^C MfeI 686 CAATTG C^AATTG MluNI 488 TGGCCA TGG^CCA MscI 488 TGGCCA TGG^CCA MunI 686 CAATTG C^AATTG MwoI 30 GCCCGAAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NheI 1584 GCTAGC G^CTAGC PpuMI 1275 AGGACCC [GA]G^G[AT]CC[CT] PshBI 1900 ATTAAT AT^TAAT Psp1406I 211 AACGTT AA^CGTT SalI 953 GTCGAC G^TCGAC SapI 18 GCTCTTC GCTCTTC(1/4) Sau96I 1276 GGACC G^G[ACGT]CC SnaBI 410 TACGTA TAC^GTA SspI 333 AATATT AAT^ATT TseI 1842 GCAGC G^C[AT]GC XbaI 174 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 57 GTCTAC GT^[AC][GT]AC 953 GTCGAC AccII 38 CGCG CG^CG 44 CGCG AlwNI 1421 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 1843 CAGCTCCTG BmyI 675 GTGCTC G[AGT]GC[ACT]^C 1484 GAGCCC BseRI 875 GAGGAG GAGGAG(10/8) 1306 GAGGAG Bsp1286I 675 GTGCTC G[AGT]GC[ACT]^C 1484 GAGCCC BsrDI 419 GCAATG GCAATG(2/0) 747 GCAATG BstUI 38 CGCG CG^CG 44 CGCG BstXI 114 CCAGGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1733 CCAACAAGCTGG EarI 19 CTCTTC CTCTTC(1/4) 1242 GAAGAG EcoT14I 1266 CCTAGG C^C[AT][AT]GG 1589 CCAAGG HgaI 9 GACGC GACGC(5/10) 42 GACGC HincII 953 GTCGAC GT[CT]^[GA]AC 1311 GTTAAC HindII 953 GTCGAC GT[CT]^[GA]AC 1311 GTTAAC HphI 1627 GGTGA GGTGA(8/7) 1744 GGTGA Ksp632I 19 CTCTTC CTCTTC(1/4) 1242 GAAGAG MslI 339 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1824 CATGAAGATG MvnI 38 CGCG CG^CG 44 CGCG NspI 882 ACATGT [GA]CATG^[CT] 1376 ACATGC PleI 1786 GACTC GAGTC(4/5) 534 GAGTC SfaNI 1654 GATGC GCATC(5/9) 268 GCATC StuI 68 AGGCCT AGG^CCT 839 AGGCCT StyI 1266 CCTAGG C^C[AT][AT]GG 1589 CCAAGG TaqI 705 TCGA T^CGA 954 TCGA TthHB8I 705 TCGA T^CGA 954 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbsI BfaI BpuAI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SmlI TfiI Tru9I Tsp45I Tsp509I TspRI