(Circular) (Six-base) MAPSORT of: pFN21AE4550.seq Check: 8200 from: 1 to: 6676 pFN21AE4550 6676 bp With 182 enzymes: SgfI * March 28, 2012 14:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4486 278 Size: 53 83 186 936 2950 2468 Fragments arranged by size: 2950 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2063 2959 4019 1937 Size: 126 896 1060 4594 Fragments arranged by size: 4594 1060 896 126 Acc65I G'GTAC_C Cuts at: 2122 3997 2122 Size: 1875 4801 AclI AA'CG_TT Cuts at: 1563 2217 4803 5176 1563 Size: 654 2586 373 3063 Fragments arranged by size: 3063 2586 654 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2011 2098 2277 2634 2711 2722 2817 3177 Size: 379 87 179 357 77 11 95 360 Cuts at: 3177 3636 3689 4739 6123 6391 1632 Size: 459 53 1050 1384 268 1917 Fragments arranged by size: 1917 1384 1050 459 379 360 357 268 179 95 87 77 53 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6479 AflIII A'CryG_T Cuts at: 1203 2887 5575 6552 1203 Size: 1684 2688 977 1327 Fragments arranged by size: 2688 1684 1327 977 AgeI A'CCGG_T Cuts at: 5507 5507 Size: 6676 AhdI GACnn_n'nnGTC Cuts at: 5405 5405 Size: 6676 AleI CACnn'nnGTG Cuts at: 1166 2348 1166 Size: 1182 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6676 AlwNI CAG_nnn'CTG Cuts at: 3431 3853 4419 5991 3431 Size: 422 566 1572 4116 Fragments arranged by size: 4116 1572 566 422 ApaLI G'TGCA_C Cuts at: 4733 5889 4733 Size: 1156 5520 ApoI r'AATT_y Cuts at: 1493 2714 2903 3014 3294 3398 3985 4211 4363 Size: 1221 189 111 280 104 587 226 152 Cuts at: 4363 6472 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1221 587 280 226 189 152 111 104 AseI AT'TA_AT Cuts at: 160 1032 3906 5230 160 Size: 872 2874 1324 1606 Fragments arranged by size: 2874 1606 1324 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 AvaI C'yCGr_G Cuts at: 1870 1943 3260 4001 1870 Size: 73 1317 741 4545 Fragments arranged by size: 4545 1317 741 73 AvrII C'CTAG_G Cuts at: 3271 3271 Size: 6676 BamHI G'GATC_C Cuts at: 4006 4373 4006 Size: 367 6309 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3997 5452 618 Size: 325 217 627 335 1875 1455 1842 Fragments arranged by size: 1875 1842 1455 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 3493 3995 1391 Size: 2102 502 4072 Fragments arranged by size: 4072 2102 502 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2306 2458 3831 928 Size: 971 407 152 1373 3773 Fragments arranged by size: 3773 1373 971 407 152 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4867 4901 1903 Size: 34 2930 34 3678 Fragments arranged by size: 3678 2930 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4568 5778 4568 Size: 1210 5466 BclI T'GATC_A Cuts at: 1548 1548 Size: 6676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5287 136 Size: 107 122 71 631 605 3615 1525 Fragments arranged by size: 3615 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6671 6671 Size: 6676 BlpI GC'TnA_GC Cuts at: 3843 4093 3843 Size: 250 6426 Bme1580I G_kGCm'C Cuts at: 1790 4737 5893 1790 Size: 2947 1156 2573 Fragments arranged by size: 2947 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6676 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4407 5365 442 Size: 416 53 83 399 24 2990 958 1753 Fragments arranged by size: 2990 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3593 1056 Size: 2537 4139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3534 3534 Size: 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5336 1239 Size: 4097 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2771 2833 2984 4801 5666 5964 2771 Size: 62 151 1817 865 298 3483 Fragments arranged by size: 3483 1817 865 298 151 62 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5339 882 Size: 617 3840 2219 Fragments arranged by size: 3840 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2417 4467 493 Size: 711 1213 2050 2702 Fragments arranged by size: 2702 2050 1213 711 BsaBI GATnn'nnATC Cuts at: 6670 6670 Size: 6676 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 4483 4865 5500 Size: 53 83 186 936 482 2468 382 635 Cuts at: 5500 275 Size: 1451 Fragments arranged by size: 2468 1451 936 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5109 5507 5781 5928 6339 5109 Size: 398 274 147 411 5446 Fragments arranged by size: 5446 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2874 2904 1178 Size: 30 165 30 1471 30 4950 Fragments arranged by size: 4950 1471 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2895 3326 1186 Size: 1709 431 4536 Fragments arranged by size: 4536 1709 431 BseYI C'CCAG_C Cuts at: 2396 3745 5879 6375 2396 Size: 1349 2134 496 2697 Fragments arranged by size: 2697 2134 1349 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6103 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4126 4887 5915 1540 Size: 275 186 2125 761 1028 2301 Fragments arranged by size: 2301 2125 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2684 4737 4822 5893 2684 Size: 2053 85 1071 3467 Fragments arranged by size: 3467 2053 1071 85 BsmI GAATG_Cn' Cuts at: 4187 4280 4187 Size: 93 6583 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2684 3493 3995 4737 4822 5893 1391 Size: 399 894 809 502 742 85 1071 2174 Fragments arranged by size: 2174 1071 894 809 742 502 399 85 BspEI T'CCGG_A Cuts at: 6339 6339 Size: 6676 BspHI T'CATG_A Cuts at: 4565 6295 4565 Size: 1730 4946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2998 4031 844 Size: 2154 1033 3489 Fragments arranged by size: 3489 2154 1033 BsrBI CCG'CTC Cuts at: 1394 1823 2023 4563 1394 Size: 429 200 2540 3507 Fragments arranged by size: 3507 2540 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 2431 2759 5171 5345 65 Size: 1183 1183 328 2412 174 1396 Fragments arranged by size: 2412 1396 1183 1183 328 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5320 5507 1700 Size: 110 3510 187 2869 Fragments arranged by size: 3510 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6676 BssSI C'ACGA_G Cuts at: 3172 4736 5748 3172 Size: 1564 1012 4100 Fragments arranged by size: 4100 1564 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6676 BstBI TT'CG_AA Cuts at: 5480 5480 Size: 6676 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6676 BstXI CCAn_nnnn'nTGG Cuts at: 2126 3745 2126 Size: 1619 5057 BstYI r'GATC_y Cuts at: 1972 4006 4373 4758 4775 6227 6313 6325 6671 Size: 2034 367 385 17 1452 86 12 346 Cuts at: 6671 1972 Size: 1977 Fragments arranged by size: 2034 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5937 Fragments arranged by size: 5937 683 56 BtsI GCAGTG_nn' Cuts at: 2137 4192 4986 5006 2137 Size: 2055 794 20 3807 Fragments arranged by size: 3807 2055 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6676 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6641 DraI TTT'AAA Cuts at: 3550 3980 3550 Size: 430 6246 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5683 809 Size: 507 4367 1802 Fragments arranged by size: 4367 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2493 4123 5012 Size: 54 1425 50 275 18 663 1630 889 Cuts at: 5012 6641 8 Size: 1629 43 Fragments arranged by size: 1630 1629 1425 889 663 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4123 1537 Size: 275 2311 4090 Fragments arranged by size: 4090 2311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 3242 4606 1353 Size: 677 1212 1364 3423 Fragments arranged by size: 3423 1364 1212 677 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5263 5637 5783 387 Size: 1064 138 3674 374 146 1280 Fragments arranged by size: 3674 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2011 2098 2277 2634 2711 2722 2817 Size: 393 379 87 179 357 77 11 95 Cuts at: 2817 3177 3636 3689 4739 5336 6123 6391 1239 Size: 360 459 53 1050 597 787 268 1524 Fragments arranged by size: 1524 1050 787 597 459 393 379 360 357 268 179 95 87 77 53 11 EcoNI CCTnn'n_nnAGG Cuts at: 3711 3711 Size: 6676 EcoO109I rG'GnC_Cy Cuts at: 3281 3281 Size: 6676 EcoRI G'AATT_C Cuts at: 3398 3985 4363 3398 Size: 587 378 5711 Fragments arranged by size: 5711 587 378 FspI TGC'GCA Cuts at: 5182 5182 Size: 6676 HaeII r_GCGC'y Cuts at: 1678 5823 1678 Size: 4145 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3035 3067 3754 3786 5317 Size: 32 135 32 1463 32 687 32 1531 Cuts at: 5317 5349 5391 5423 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1531 1463 687 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2960 3318 4020 4266 669 Size: 988 281 1022 358 702 246 3079 Fragments arranged by size: 3079 1022 988 702 358 281 246 HindIII A'AGCT_T Cuts at: 748 2163 748 Size: 1415 5261 HpaI GTT'AAC Cuts at: 3318 4266 3318 Size: 948 5728 KpnI G_GTAC'C Cuts at: 2126 4001 2126 Size: 1875 4801 MfeI C'AATT_G Cuts at: 2691 4275 2691 Size: 1584 5092 MluI A'CGCG_T Cuts at: 6552 6552 Size: 6676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2036 2696 3464 5790 5974 1305 Size: 731 660 768 2326 184 2007 Fragments arranged by size: 2326 2007 768 731 660 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 10 Size: 54 1425 343 663 4191 Fragments arranged by size: 4191 1425 663 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 3833 4634 4993 5152 518 Size: 648 1182 1485 801 359 159 2042 Fragments arranged by size: 2042 1485 1182 801 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4092 4769 5917 6162 1606 Size: 2486 677 1148 245 2120 Fragments arranged by size: 2486 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6124 NdeI CA'TA_TG Cuts at: 387 387 Size: 6676 NheI G'CTAG_C Cuts at: 1052 3589 1052 Size: 2537 4139 NotI GC'GGCC_GC Cuts at: 4123 4123 Size: 6676 NspI r_CATG'y Cuts at: 1132 1229 2891 3385 4034 5579 1132 Size: 97 1662 494 649 1545 2229 Fragments arranged by size: 2229 1662 1545 649 494 97 PasI CC'CwG_GG Cuts at: 1348 2596 1348 Size: 1248 5428 PciI A'CATG_T Cuts at: 2887 5575 2887 Size: 2688 3988 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6676 PmeI GTTT'AAAC Cuts at: 3980 3980 Size: 6676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4745 4777 6258 6290 4745 Size: 32 1481 32 5131 Fragments arranged by size: 5131 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3281 3281 Size: 6676 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6676 PsiI TTA'TAA Cuts at: 2195 2826 4246 2195 Size: 631 1420 4625 Fragments arranged by size: 4625 1420 631 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3205 3237 3205 Size: 32 6644 PstI C_TGCA'G Cuts at: 830 4028 830 Size: 3198 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6676 SalI G'TCGA_C Cuts at: 1936 2958 4018 1936 Size: 1022 1060 4594 Fragments arranged by size: 4594 1060 1022 SapI GCTCTTCn'nnn_ Cuts at: 2030 2030 Size: 6676 SbfI CC_TGCA'GG Cuts at: 4028 4028 Size: 6676 ScaI AGT'ACT Cuts at: 4924 6636 4924 Size: 1712 4964 SfcI C'TryA_G Cuts at: 826 1045 4024 5159 5840 6031 826 Size: 219 2979 1135 681 191 1471 Fragments arranged by size: 2979 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6676 SmaI CCC'GGG Cuts at: 1872 4003 1872 Size: 2131 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2786 2848 2999 4780 5681 5943 Size: 197 926 843 62 151 1781 901 262 Cuts at: 5943 820 Size: 1553 Fragments arranged by size: 1781 1553 926 901 843 262 197 151 62 SnaBI TAC'GTA Cuts at: 493 2417 4467 493 Size: 1924 2050 2702 Fragments arranged by size: 2702 2050 1924 SpeI A'CTAG_T Cuts at: 152 152 Size: 6676 SphI G_CATG'C Cuts at: 1132 4034 1132 Size: 2902 3774 SspI AAT'ATT Cuts at: 5 52 2340 4600 5 Size: 47 2288 2260 2081 Fragments arranged by size: 2288 2260 2081 47 StuI AGG'CCT Cuts at: 1517 2075 2846 1517 Size: 558 771 5347 Fragments arranged by size: 5347 771 558 StyI C'CwwG_G Cuts at: 513 1065 3271 3594 4115 513 Size: 552 2206 323 521 3074 Fragments arranged by size: 3074 2206 552 521 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3737 4703 4720 4873 5058 5503 325 Size: 1255 2157 966 17 153 185 445 1498 Fragments arranged by size: 2157 1498 1255 966 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4922 6634 96 Size: 274 80 33 51 1443 2945 1712 138 Fragments arranged by size: 2945 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6636 6668 6636 Size: 32 6644 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6676 XbaI T'CTAG_A Cuts at: 2179 4012 2179 Size: 1833 4843 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6676 XmaI C'CCGG_G Cuts at: 1870 4001 1870 Size: 2131 4545 XmnI GAAnn'nnTTC Cuts at: 4805 4805 Size: 6676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4484 276 Size: 53 83 186 936 2950 2468 Fragments arranged by size: 2950 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI