################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AfaI AgeI Alw44I AocI ApaI ApaLI AscI Asp718I AspHI AviII AvrII BamHI BanII BbeI BbrPI BcgI BciVI BclI BcnI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 15 GACGTC GACGT^C AcyI 15 GACGTC G[GA]^CG[CT]C AflII 1306 CTTAAG C^TTAAG AflIII 1162 ACACGT A^C[GA][CT]GT Aor51HI 56 AGCGCT AGC^GCT AseI 907 ATTAAT AT^TAAT AsnI 907 ATTAAT AT^TAAT Asp700I 1001 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 103 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 634 CTCGAG C^[CT]CG[GA]G BalI 950 TGGCCA TGG^CCA BanI 319 GGCACC G^G[CT][GA]CC BfaI 565 CTAG C^TAG BfrI 1306 CTTAAG C^TTAAG BglII 500 AGATCT A^GATCT BsaBI 391 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 15 GACGTC G[GA]^CG[CT]C BsaI 166 GAGACC GGTCTC(1/5) BsgI 1126 GTGCAG GTGCAG(16/14) BsmI 415 GAATGC GAATGC(1/-1) BsoBI 634 CTCGAG C^[CT]CG[GA]G BspMI 36 GCAGGT ACCTGC(4/8) BsrDI 1102 GCAATG GCAATG(2/0) BssSI 84 CACGAG CACGAG(-5/-1) BstXI 953 CCAATAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 57 GCGC GCG^C DraI 706 TTTAAA TTT^AAA DraII 359 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 404 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 950 TGGCCA [CT]^GGCC[GA] Eco47III 56 AGCGCT AGC^GCT EcoO109I 359 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 323 CCATGG C^C[AT][AT]GG HaeII 56 AGCGCT [GA]GCGC^[CT] HgaI 774 GACGC GACGC(5/10) HhaI 57 GCGC GCG^C Hin1I 15 GACGTC G[GA]^CG[CT]C HinP1I 57 GCGC G^CGC MaeI 565 CTAG C^TAG MamI 391 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 950 TGGCCA TGG^CCA MscI 950 TGGCCA TGG^CCA MslI 33 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 323 CCATGG C^CATGG PaeR7I 634 CTCGAG C^TCGAG PflFI 103 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1018 GAGTC GAGTC(4/5) PpuMI 359 GGGACCT [GA]G^G[AT]CC[CT] PshBI 907 ATTAAT AT^TAAT PstI 60 CTGCAG CTGCA^G StyI 323 CCATGG C^C[AT][AT]GG TaqI 635 TCGA T^CGA Tth111I 103 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 635 TCGA T^CGA XcmI 881 CCAGTGTTCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 634 CTCGAG C^TCGAG XmnI 1001 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 703 GAATTT [GA]^AATT[CT] 970 AAATTT AhdI 344 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 370 GACATCATGTC ApoI 703 GAATTT [GA]^AATT[CT] 970 AAATTT AspEI 344 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 370 GACATCATGTC BbvCI 333 CCTCAGC CCTCAGC(-5/-2) 649 GCTGAGG BpmI 721 CTGGAG CTGGAG(16/14) 1015 CTGGAG BsmFI 359 GGGAC GGGAC(10/14) 1082 GGGAC BstYI 95 AGATCC [GA]^GATC[CT] 500 AGATCT DsaI 323 CCATGG C^C[GA][CT]GG 890 CCGTGG Eam1105I 344 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 370 GACATCATGTC MflI 95 AGATCC [GA]^GATC[CT] 500 AGATCT SfaNI 759 GATGC GCATC(5/9) 307 GCATC SfcI 60 CTGCAG C^T[GA][CT]AG 1179 CTACAG TfiI 242 GATTC G^A[AT]TC 1302 GAATC XhoII 95 AGATCC [GA]^GATC[CT] 500 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BbsI BbvI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PflMI PvuII Sau3AI Sau96I ScrFI SmlI Tru9I TseI Tsp45I Tsp509I TspRI Van91I