(Circular) (Six-base) MAPSORT of: pFN21AE4431.seq Check: 1059 from: 1 to: 6055 pFN21AE4431 6055 bp With 182 enzymes: SgfI * March 28, 2012 14:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2032 2032 Size: 6055 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2024 3865 278 Size: 53 83 186 936 488 1841 2468 Fragments arranged by size: 2468 1841 936 488 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 1937 Size: 1461 4594 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2372 2765 2816 2851 4118 5502 5770 1632 Size: 740 393 51 35 1267 1384 268 1917 Fragments arranged by size: 1917 1384 1267 740 393 268 51 35 AfeI AGC'GCT Cuts at: 2063 2063 Size: 6055 AflII C'TTAA_G Cuts at: 820 1017 3311 820 Size: 197 2294 3564 Fragments arranged by size: 3564 2294 197 AflIII A'CryG_T Cuts at: 1203 3167 4954 5931 1203 Size: 1964 1787 977 1327 Fragments arranged by size: 1964 1787 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 2354 2380 4784 2354 Size: 26 2404 3625 Fragments arranged by size: 3625 2404 26 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6055 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AlwNI CAG_nnn'CTG Cuts at: 2077 2596 2725 3798 5370 2077 Size: 519 129 1073 1572 2762 Fragments arranged by size: 2762 1572 1073 519 129 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 2708 2975 3364 3590 3742 5851 1493 Size: 1215 267 389 226 152 2109 1697 Fragments arranged by size: 2109 1697 1215 389 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 2913 4609 160 Size: 872 1881 1696 1606 Fragments arranged by size: 1881 1696 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2639 3380 1870 Size: 73 696 741 4545 Fragments arranged by size: 4545 741 696 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 3376 4831 618 Size: 325 217 627 537 1052 1455 1842 Fragments arranged by size: 1842 1455 1052 627 537 325 217 BanII G_rGCy'C Cuts at: 1391 3374 1391 Size: 1983 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2353 2607 3170 3222 928 Size: 971 454 254 563 52 3761 Fragments arranged by size: 3761 971 563 454 254 52 BbvCI CC'TCA_GC Cuts at: 2339 2655 2339 Size: 316 5739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4246 4280 1903 Size: 34 2309 34 3678 Fragments arranged by size: 3678 2309 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4666 136 Size: 107 122 71 631 605 2994 1525 Fragments arranged by size: 2994 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2505 6050 2505 Size: 3545 2510 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 4116 5272 1790 Size: 2326 1156 2573 Fragments arranged by size: 2573 2326 1156 BmgBI CAC'GTC Cuts at: 1318 2605 1318 Size: 1287 4768 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2370 2880 3250 Size: 416 53 83 399 24 953 510 370 Cuts at: 3250 3786 4744 442 Size: 536 958 1753 Fragments arranged by size: 1753 958 953 536 510 416 399 370 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2209 2209 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2747 3041 4715 1239 Size: 1508 294 1674 2579 Fragments arranged by size: 2579 1674 1508 294 Bpu10I CC'TnA_GC Cuts at: 2339 2655 2339 Size: 316 5739 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3275 4180 5045 5343 3275 Size: 905 865 298 3987 Fragments arranged by size: 3987 905 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2165 4718 882 Size: 617 666 2553 2219 Fragments arranged by size: 2553 2219 666 617 BsaAI yAC'GTr Cuts at: 493 1204 3846 493 Size: 711 2642 2702 Fragments arranged by size: 2702 2642 711 BsaBI GATnn'nnATC Cuts at: 2400 6049 2400 Size: 3649 2406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 3862 4244 4879 Size: 53 83 186 936 488 1841 382 635 Cuts at: 4879 275 Size: 1451 Fragments arranged by size: 1841 1451 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4488 4886 5160 5307 5718 4488 Size: 398 274 147 411 4825 Fragments arranged by size: 4825 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3038 3068 1178 Size: 30 165 30 1635 30 4165 Fragments arranged by size: 4165 1635 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2211 2216 2588 2591 3284 1186 Size: 1025 5 372 3 693 3957 Fragments arranged by size: 3957 1025 693 372 5 3 BseYI C'CCAG_C Cuts at: 2257 2872 5258 5754 2257 Size: 615 2386 496 2558 Fragments arranged by size: 2558 2386 615 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3152 1163 Size: 573 1416 4066 Fragments arranged by size: 4066 1416 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4266 5294 1540 Size: 275 186 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4116 4201 5272 4116 Size: 85 1071 4899 Fragments arranged by size: 4899 1071 85 BsmI GAATG_Cn' Cuts at: 2426 3566 3659 2426 Size: 1140 93 4822 Fragments arranged by size: 4822 1140 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3374 4116 4201 5272 1391 Size: 399 1584 742 85 1071 2174 Fragments arranged by size: 2174 1584 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2032 3410 844 Size: 1188 1378 3489 Fragments arranged by size: 3489 1378 1188 BsrBI CCG'CTC Cuts at: 1394 1823 3942 1394 Size: 429 2119 3507 Fragments arranged by size: 3507 2119 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3114 4550 4724 65 Size: 1183 1866 1436 174 1396 Fragments arranged by size: 1866 1436 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4699 4886 1700 Size: 110 2889 187 2869 Fragments arranged by size: 2889 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 2089 4115 5127 2089 Size: 2026 1012 3017 Fragments arranged by size: 3017 2026 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstXI CCAn_nnnn'nTGG Cuts at: 2965 2965 Size: 6055 BstYI r'GATC_y Cuts at: 1972 2100 2505 3385 3752 4137 4154 5606 5692 Size: 128 405 880 367 385 17 1452 86 Cuts at: 5692 5704 6050 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 880 405 385 367 346 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2328 2895 513 Size: 552 939 324 567 3673 Fragments arranged by size: 3673 939 567 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 3235 3281 3571 4365 4385 3235 Size: 46 290 794 20 4905 Fragments arranged by size: 4905 794 290 46 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 2713 3359 2713 Size: 646 5409 DraIII CAC_nnn'GTG Cuts at: 2414 2414 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2955 3502 4391 Size: 54 1425 50 275 18 1125 547 889 Cuts at: 4391 6020 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1125 889 547 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2741 3141 3985 1353 Size: 825 563 400 844 3423 Fragments arranged by size: 3423 844 825 563 400 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4642 5016 5162 387 Size: 1064 138 3053 374 146 1280 Fragments arranged by size: 3053 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 2747 2765 2816 2851 3041 4118 Size: 393 740 375 18 51 35 190 1077 Cuts at: 4118 4715 5502 5770 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1077 787 740 597 393 375 268 190 51 35 18 EcoO109I rG'GnC_Cy Cuts at: 2365 2365 Size: 6055 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 2065 5202 1678 Size: 387 3137 2531 Fragments arranged by size: 3137 2531 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 2340 2366 2372 Size: 32 135 32 650 32 86 26 6 Cuts at: 2372 2398 2656 2688 3271 3303 4696 4728 4770 Size: 26 258 32 583 32 1393 32 42 Cuts at: 4770 4802 1373 Size: 32 2626 Fragments arranged by size: 2626 1393 650 583 258 135 86 42 32 32 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 5169 5353 1305 Size: 744 3120 184 2007 Fragments arranged by size: 3120 2007 744 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2957 10 Size: 54 1425 343 1125 3108 Fragments arranged by size: 3108 1425 1125 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 4013 4372 4531 518 Size: 648 876 1971 359 159 2042 Fragments arranged by size: 2042 1971 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2446 2725 2785 3471 4148 5296 5541 1606 Size: 840 279 60 686 677 1148 245 2120 Fragments arranged by size: 2120 1148 840 686 677 279 245 60 NcoI C'CATG_G Cuts at: 513 1065 2004 2328 513 Size: 552 939 324 4240 Fragments arranged by size: 4240 939 552 324 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 3413 4958 1132 Size: 97 2184 1545 2229 Fragments arranged by size: 2229 2184 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 2953 3109 1108 Size: 1488 357 156 4054 Fragments arranged by size: 4054 1488 357 156 PfoI T'CCnGG_A Cuts at: 2103 2121 2589 3120 2103 Size: 18 468 531 5038 Fragments arranged by size: 5038 531 468 18 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2365 2365 Size: 6055 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3348 3625 3348 Size: 277 5778 PstI C_TGCA'G Cuts at: 830 2069 3407 830 Size: 1239 1338 3478 Fragments arranged by size: 3478 1338 1239 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2446 2725 2785 2446 Size: 279 60 5716 Fragments arranged by size: 5716 279 60 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2065 3184 3403 4538 5219 5410 826 Size: 219 1020 1119 219 1135 681 191 1471 Fragments arranged by size: 1471 1135 1119 1020 681 219 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 3290 3311 4159 5060 5322 Size: 197 926 696 651 21 848 901 262 Cuts at: 5322 820 Size: 1553 Fragments arranged by size: 1553 926 901 848 696 651 262 197 21 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 2004 2328 3494 513 Size: 552 939 324 1166 3074 Fragments arranged by size: 3074 1166 939 552 324 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2421 3319 4082 4099 4252 4437 4882 Size: 1255 841 898 763 17 153 185 445 Cuts at: 4882 325 Size: 1498 Fragments arranged by size: 1498 1255 898 841 763 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4301 6013 96 Size: 274 80 33 51 1443 2324 1712 138 Fragments arranged by size: 2324 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6015 6047 6015 Size: 32 6023 Tth111I GACn'n_nGTC Cuts at: 1316 2111 1316 Size: 795 5260 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2893 2893 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 2639 1943 Size: 696 5359 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 3010 4184 3010 Size: 1174 4881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2022 3863 276 Size: 53 83 186 936 488 1841 2468 Fragments arranged by size: 2468 1841 936 488 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI