(Linear) (Six-base) MAPSORT of: KIEE1489.seq Check: 7090 from: 1 to: 1365 KIEE1489.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 508 1365 Size: 508 857 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 257 801 837 997 1365 Size: 257 544 36 160 368 Fragments arranged by size: 544 368 257 160 36 AflIII A'CryG_T Cuts at: 0 1266 1365 Size: 1266 99 AleI CACnn'nnGTG Cuts at: 0 631 1365 Size: 631 734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 649 1365 Size: 649 716 AlwNI CAG_nnn'CTG Cuts at: 0 293 443 1365 Size: 293 150 922 Fragments arranged by size: 922 293 150 ApaLI G'TGCA_C Cuts at: 0 460 495 570 1365 Size: 460 35 75 795 Fragments arranged by size: 795 460 75 35 ApoI r'AATT_y Cuts at: 0 1117 1365 Size: 1117 248 AvaI C'yCGr_G Cuts at: 0 222 1365 Size: 222 1143 AvrII C'CTAG_G Cuts at: 0 99 1131 1365 Size: 99 1032 234 Fragments arranged by size: 1032 234 99 BanI G'GyrC_C Cuts at: 0 625 942 1127 1365 Size: 625 317 185 238 Fragments arranged by size: 625 317 238 185 BbeI G_GCGC'C Cuts at: 0 1131 1365 Size: 1131 234 BbsI GAAGACnn'nnnn_ Cuts at: 0 1348 1365 Size: 1348 17 BbvCI CC'TCA_GC Cuts at: 0 298 1365 Size: 298 1067 BciVI GTATCCnnnnn_n' Cuts at: 0 225 1365 Size: 225 1140 BglII A'GATC_T Cuts at: 0 338 1365 Size: 338 1027 BlpI GC'TnA_GC Cuts at: 0 1138 1365 Size: 1138 227 Bme1580I G_kGCm'C Cuts at: 0 464 499 574 613 866 1100 1365 Size: 464 35 75 39 253 234 265 Fragments arranged by size: 464 265 253 234 75 39 35 BmrI ACTGGGnnnn_n' Cuts at: 0 860 1346 1365 Size: 860 486 19 Fragments arranged by size: 860 486 19 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 276 555 1365 Size: 276 279 810 Fragments arranged by size: 810 279 276 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 586 812 867 1183 1239 1273 1321 Size: 67 519 226 55 316 56 34 48 Cuts at: 1321 1365 Size: 44 Fragments arranged by size: 519 316 226 67 56 55 48 44 34 Bpu10I CC'TnA_GC Cuts at: 0 298 1365 Size: 298 1067 BsaHI Gr'CG_yC Cuts at: 0 1128 1365 Size: 1128 237 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 26 56 1256 1286 1365 Size: 26 30 1200 30 79 Fragments arranged by size: 1200 79 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 670 1327 1365 Size: 670 657 38 Fragments arranged by size: 670 657 38 BseYI C'CCAG_C Cuts at: 0 1260 1290 1365 Size: 1260 30 75 Fragments arranged by size: 1260 75 30 BsiEI CG_ry'CG Cuts at: 0 1158 1365 Size: 1158 207 BsiHKAI G_wGCw'C Cuts at: 0 44 464 499 574 1274 1365 Size: 44 420 35 75 700 91 Fragments arranged by size: 700 420 91 75 44 35 Bsp1286I G_dGCh'C Cuts at: 0 44 464 499 574 613 866 1100 1274 Size: 44 420 35 75 39 253 234 174 Cuts at: 1274 1365 Size: 91 Fragments arranged by size: 420 253 234 174 91 75 44 39 35 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 32 499 508 1295 1365 Size: 32 467 9 787 70 Fragments arranged by size: 787 467 70 32 9 BsrBI CCG'CTC Cuts at: 0 257 1365 Size: 257 1108 BsrFI r'CCGG_y Cuts at: 0 395 1365 Size: 395 970 BsrGI T'GTAC_A Cuts at: 0 203 1365 Size: 203 1162 BssSI C'ACGA_G Cuts at: 0 1147 1365 Size: 1147 218 BstAPI GCAn_nnn'nTGC Cuts at: 0 443 1365 Size: 443 922 BstYI r'GATC_y Cuts at: 0 338 1365 Size: 338 1027 Bsu36I CC'TnA_GG Cuts at: 0 72 1365 Size: 72 1293 BtgI C'CryG_G Cuts at: 0 1082 1234 1365 Size: 1082 152 131 Fragments arranged by size: 1082 152 131 BtsI GCAGTG_nn' Cuts at: 0 291 1365 Size: 291 1074 ClaI AT'CG_AT Cuts at: 0 1154 1365 Size: 1154 211 DraIII CAC_nnn'GTG Cuts at: 0 371 494 1365 Size: 371 123 871 Fragments arranged by size: 871 371 123 EarI CTCTTCn'nnn_ Cuts at: 0 109 348 688 1365 Size: 109 239 340 677 Fragments arranged by size: 677 340 239 109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 257 586 801 812 837 867 997 Size: 67 190 329 215 11 25 30 130 Cuts at: 997 1183 1239 1273 1321 1365 Size: 186 56 34 48 44 Fragments arranged by size: 329 215 190 186 130 67 56 48 44 34 30 25 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 98 1189 1365 Size: 98 1091 176 Fragments arranged by size: 1091 176 98 EcoRI G'AATT_C Cuts at: 0 1117 1365 Size: 1117 248 HaeII r_GCGC'y Cuts at: 0 1034 1131 1182 1321 1365 Size: 1034 97 51 139 44 Fragments arranged by size: 1034 139 97 51 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 281 313 1256 1288 1365 Size: 281 32 943 32 77 Fragments arranged by size: 943 281 77 32 32 HindIII A'AGCT_T Cuts at: 0 813 1365 Size: 813 552 KasI G'GCGC_C Cuts at: 0 1127 1365 Size: 1127 238 MfeI C'AATT_G Cuts at: 0 432 1365 Size: 432 933 MluI A'CGCG_T Cuts at: 0 1266 1365 Size: 1266 99 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1016 1365 Size: 1016 349 MslI CAynn'nnrTG Cuts at: 0 631 1365 Size: 631 734 MspA1I CmG'CkG Cuts at: 0 302 1236 1365 Size: 302 934 129 Fragments arranged by size: 934 302 129 NarI GG'CG_CC Cuts at: 0 1128 1365 Size: 1128 237 NspI r_CATG'y Cuts at: 0 1209 1365 Size: 1209 156 PasI CC'CwG_GG Cuts at: 0 846 1365 Size: 846 519 PfoI T'CCnGG_A Cuts at: 0 44 1365 Size: 44 1321 PstI C_TGCA'G Cuts at: 0 244 382 1172 1365 Size: 244 138 790 193 Fragments arranged by size: 790 244 193 138 PvuI CG_AT'CG Cuts at: 0 1158 1365 Size: 1158 207 PvuII CAG'CTG Cuts at: 0 302 1365 Size: 302 1063 SacII CC_GC'GG Cuts at: 0 1237 1365 Size: 1237 128 SfcI C'TryA_G Cuts at: 0 240 378 543 1168 1365 Size: 240 138 165 625 197 Fragments arranged by size: 625 240 197 165 138 SfoI GGC'GCC Cuts at: 0 1129 1365 Size: 1129 236 SmaI CCC'GGG Cuts at: 0 224 1365 Size: 224 1141 SphI G_CATG'C Cuts at: 0 1209 1365 Size: 1209 156 StyI C'CwwG_G Cuts at: 0 99 135 1131 1365 Size: 99 36 996 234 Fragments arranged by size: 996 234 99 36 TatI w'GTAC_w Cuts at: 0 203 1365 Size: 203 1162 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 26 58 355 387 1365 Size: 26 32 297 32 978 Fragments arranged by size: 978 297 32 32 26 XmaI C'CCGG_G Cuts at: 0 222 1365 Size: 222 1143 XmnI GAAnn'nnTTC Cuts at: 0 1117 1365 Size: 1117 248 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI AvrII BanI BbeI BbsI BbvCI BciVI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI ClaI DraIII EarI Eco57MI EcoNI EcoRI HaeII Hin4I HindIII KasI MfeI MluI MmeI MslI MspA1I NarI NspI PasI PfoI PstI PvuI PvuII SacII SfcI SfoI SmaI SphI StyI TatI TstI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI BaeI BamHI BanII BcgI BclI BfrBI BglI BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BstBI BstEII BstXI BstZ17I BtgZI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI ZraI