Restriction map for
pFN21AE1390
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Aor51HI 764 AGCGCT AGC^GCT
AvaI 298 CCCGAG C^[CT]CG[GA]G
BbsI 94 GTCTTC GAAGAC(2/6)
BbvI 694 GCTGC GCAGC(8/12)
BciVI 210 GTATCC GTATCC(6/5)
BcnI 351 CCGGG CC^[GC]GG
BfaI 971 CTAG C^TAG
BglI 409 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 257 AGATCT A^GATCT
BpuAI 94 GTCTTC GAAGAC(2/6)
BsoBI 298 CCCGAG C^[CT]CG[GA]G
BsrDI 758 GCAATG GCAATG(2/0)
BsrFI 61 GCCGGC [GA]^CCGG[CT]
BsrI 239 ACTGG ACTGG(1/-1)
Cfr10I 61 GCCGGC [GA]^CCGG[CT]
DsaI 118 CCGCGG C^C[GA][CT]GG
Eco47III 764 AGCGCT AGC^GCT
EcoRV 868 GATATC GAT^ATC
EcoT14I 341 CCAAGG C^C[AT][AT]GG
HaeII 764 AGCGCT [GA]GCGC^[CT]
KspI 118 CCGCGG CCGC^GG
MaeI 971 CTAG C^TAG
NaeI 61 GCCGGC GCC^GGC
NciI 351 CCGGG CC^[GC]GG
NgoMIV 61 GCCGGC G^CCGGC
PvuII 692 CAGCTG CAG^CTG
SacI 283 GAGCTC GAGCT^C
SacII 118 CCGCGG CCGC^GG
SapI 717 GAAGAGC GCTCTTC(1/4)
SnaBI 163 TACGTA TAC^GTA
StyI 341 CCAAGG C^C[AT][AT]GG
TaqI 218 TCGA T^CGA
TfiI 814 GAATC G^A[AT]TC
TseI 694 GCTGC G^C[AT]GC
Tsp509I 627 AATT ^AATT
TthHB8I 218 TCGA T^CGA
XcmI 527 CCATCATGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 579 GATCC GGATC(4/5)
540 GGATC
AspHI 283 GAGCTC G[AT]GC[AT]^C
304 GAGCAC
AvaII 555 GGACC G^G[AT]CC
879 GGACC
BanII 283 GAGCTC G[GA]GC[CT]^C
1032 GGGCTC
BpmI 712 CTGGAG CTGGAG(16/14)
905 CTGGAG
BsaAI 163 TACGTA [CT]AC^GT[GA]
403 TACGTG
BsaI 16 GAGACC GGTCTC(1/5)
379 GAGACC
BsiHKAI 283 GAGCTC G[AT]GC[AT]^C
304 GAGCAC
BspHI 572 TCATGA T^CATGA
851 TCATGA
BstXI 20 CCACCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
398 CCATGTACGTGG
HapII 62 CCGG C^CGG
351 CCGG
HgaI 79 GACGC GACGC(5/10)
534 GCGTC
HpaII 62 CCGG C^CGG
351 CCGG
MseI 641 TTAA T^TAA
986 TTAA
MspI 62 CCGG C^CGG
351 CCGG
PpuMI 554 GGGACCT [GA]G^G[AT]CC[CT]
878 AGGACCT
RcaI 572 TCATGA T^CATGA
851 TCATGA
Tru9I 641 TTAA T^TAA
986 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI BalI
BanI BmyI BsaJI BseRI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI Tsp45I
TspRI XhoII