Restriction map for
pFN21AB8851
(Circular) (Six-base) MAPSORT of: pFN21AB8851.seq Check: 7604 from: 1 to: 4924
pFN21AB8851 4924 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2734 278
Size: 53 83 186 936 1198 2468
Fragments arranged by size:
2468 1198 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2267 1937
Size: 330 4594
Acc65I G'GTAC_C
Cuts at: 2245 2245
Size: 4924
AclI AA'CG_TT
Cuts at: 1563 2143 3051 3424 1563
Size: 580 908 373 3063
Fragments arranged by size:
3063 908 580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 2987 4371 4639 1632
Size: 504 851 1384 268 1917
Fragments arranged by size:
1917 1384 851 504 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4727
AflIII A'CryG_T
Cuts at: 1203 3823 4800 1203
Size: 2620 977 1327
Fragments arranged by size:
2620 1327 977
AgeI A'CCGG_T
Cuts at: 3755 3755
Size: 4924
AhdI GACnn_n'nnGTC
Cuts at: 3653 3653
Size: 4924
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4924
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4924
AlwNI CAG_nnn'CTG
Cuts at: 2667 4239 2667
Size: 1572 3352
ApaLI G'TGCA_C
Cuts at: 2981 4137 2981
Size: 1156 3768
ApoI r'AATT_y
Cuts at: 1493 2172 2233 2459 2611 4720 1493
Size: 679 61 226 152 2109 1697
Fragments arranged by size:
2109 1697 679 226 152 61
AseI AT'TA_AT
Cuts at: 160 1032 3478 160
Size: 872 2446 1606
Fragments arranged by size:
2446 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4924
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 1870
Size: 73 306 4545
Fragments arranged by size:
4545 306 73
BamHI G'GATC_C
Cuts at: 2155 2254 2621 2155
Size: 99 367 4458
Fragments arranged by size:
4458 367 99
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2245 3700 618
Size: 325 217 627 458 1455 1842
Fragments arranged by size:
1842 1455 627 458 325 217
BanII G_rGCy'C
Cuts at: 1391 2243 1391
Size: 852 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2187 928
Size: 971 288 3665
Fragments arranged by size:
3665 971 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3115 3149 1903
Size: 34 50 34 1094 34 3678
Fragments arranged by size:
3678 1094 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2816 4026 2816
Size: 1210 3714
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4924
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3535 136
Size: 107 122 71 631 605 1863 1525
Fragments arranged by size:
1863 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4919 4919
Size: 4924
BlpI GC'TnA_GC
Cuts at: 2341 2341
Size: 4924
Bme1580I G_kGCm'C
Cuts at: 1790 2985 4141 1790
Size: 1195 1156 2573
Fragments arranged by size:
2573 1195 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4924
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2655 3613 442
Size: 416 53 83 399 24 1238 958 1753
Fragments arranged by size:
1753 1238 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2169 3584 1239
Size: 930 1415 2579
Fragments arranged by size:
2579 1415 930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3049 3914 4212 3049
Size: 865 298 3761
Fragments arranged by size:
3761 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3587 882
Size: 617 2088 2219
Fragments arranged by size:
2219 2088 617
BsaAI yAC'GTr
Cuts at: 493 1204 2715 493
Size: 711 1511 2702
Fragments arranged by size:
2702 1511 711
BsaBI GATnn'nnATC
Cuts at: 4918 4918
Size: 4924
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2731 3113 3748 275
Size: 53 83 186 936 1198 382 635 1451
Fragments arranged by size:
1451 1198 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3357 3755 4029 4176 4587 3357
Size: 398 274 147 411 3694
Fragments arranged by size:
3694 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4699
Fragments arranged by size:
4699 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4924
BseYI C'CCAG_C
Cuts at: 4127 4623 4127
Size: 496 4428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2374 3135 4163 1540
Size: 275 186 373 761 1028 2301
Fragments arranged by size:
2301 1028 761 373 275 186
BsiHKAI G_wGCw'C
Cuts at: 2985 3070 4141 2985
Size: 85 1071 3768
Fragments arranged by size:
3768 1071 85
BsmI GAATG_Cn'
Cuts at: 2435 2528 2435
Size: 93 4831
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2985 3070 4141 1391
Size: 399 453 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 399 85
BspEI T'CCGG_A
Cuts at: 4587 4587
Size: 4924
BspHI T'CATG_A
Cuts at: 2813 4543 2813
Size: 1730 3194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2279 844
Size: 1435 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2811 1394
Size: 429 988 3507
Fragments arranged by size:
3507 988 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3419 3593 65
Size: 1183 2171 174 1396
Fragments arranged by size:
2171 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3568 3755 1700
Size: 110 1758 187 2869
Fragments arranged by size:
2869 1758 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4924
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4924
BssSI C'ACGA_G
Cuts at: 2984 3996 2984
Size: 1012 3912
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4924
BstBI TT'CG_AA
Cuts at: 3728 3728
Size: 4924
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4924
BstYI r'GATC_y
Cuts at: 1972 2155 2254 2621 3006 3023 4475 4561 4573
Size: 183 99 367 385 17 1452 86 12
Cuts at: 4573 4919 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 183 99 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4372
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4185
Fragments arranged by size:
4185 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 3234 3254 2440
Size: 794 20 4110
Fragments arranged by size:
4110 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4924
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4889
DraI TTT'AAA
Cuts at: 2197 2228 2197
Size: 31 4893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3931 809
Size: 507 2615 1802
Fragments arranged by size:
2615 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2371 3260 4889
Size: 54 1425 50 275 18 541 889 1629
Cuts at: 4889 8
Size: 43
Fragments arranged by size:
1629 1425 889 541 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2371 1537
Size: 275 559 4090
Fragments arranged by size:
4090 559 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2854 1353
Size: 1501 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3511 3885 4031 387
Size: 1064 138 1922 374 146 1280
Fragments arranged by size:
1922 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2169 2987 3584 4371 4639 1239
Size: 393 504 33 818 597 787 268 1524
Fragments arranged by size:
1524 818 787 597 504 393 268 33
EcoRI G'AATT_C
Cuts at: 2172 2233 2611 2172
Size: 61 378 4485
Fragments arranged by size:
4485 378 61
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4924
FspI TGC'GCA
Cuts at: 3430 3430
Size: 4924
HaeII r_GCGC'y
Cuts at: 1678 4071 1678
Size: 2393 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3565 3597 3639 3671 1373
Size: 32 135 32 1993 32 42 32 2626
Fragments arranged by size:
2626 1993 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2268 2514 669
Size: 988 281 330 246 3079
Fragments arranged by size:
3079 988 330 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4924
HpaI GTT'AAC
Cuts at: 2514 2514
Size: 4924
KpnI G_GTAC'C
Cuts at: 2249 2249
Size: 4924
MfeI C'AATT_G
Cuts at: 2523 2523
Size: 4924
MluI A'CGCG_T
Cuts at: 4800 4800
Size: 4924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4038 4222 1305
Size: 2733 184 2007
Fragments arranged by size:
2733 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3102
Fragments arranged by size:
3102 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2882 3241 3400 518
Size: 648 1716 359 159 2042
Fragments arranged by size:
2042 1716 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2211 2340 3017 4165 4410 1606
Size: 605 129 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 605 245 129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4372
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4924
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4924
NotI GC'GGCC_GC
Cuts at: 2371 2371
Size: 4924
NspI r_CATG'y
Cuts at: 1132 1229 2282 3827 1132
Size: 97 1053 1545 2229
Fragments arranged by size:
2229 1545 1053 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4924
PciI A'CATG_T
Cuts at: 3823 3823
Size: 4924
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4924
PmeI GTTT'AAAC
Cuts at: 2228 2228
Size: 4924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 4506 4538 2993
Size: 32 1481 32 3379
Fragments arranged by size:
3379 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4924
PsiI TTA'TAA
Cuts at: 2494 2494
Size: 4924
PstI C_TGCA'G
Cuts at: 830 2276 830
Size: 1446 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4924
PvuII CAG'CTG
Cuts at: 2211 2211
Size: 4924
SalI G'TCGA_C
Cuts at: 1936 2266 1936
Size: 330 4594
SbfI CC_TGCA'GG
Cuts at: 2276 2276
Size: 4924
ScaI AGT'ACT
Cuts at: 3172 4884 3172
Size: 1712 3212
SfcI C'TryA_G
Cuts at: 826 1045 2272 3407 4088 4279 826
Size: 219 1227 1135 681 191 1471
Fragments arranged by size:
1471 1227 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4924
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4924
SmaI CCC'GGG
Cuts at: 1872 2251 1872
Size: 379 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3028 3929 4191 820
Size: 197 926 1085 901 262 1553
Fragments arranged by size:
1553 1085 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2715 493
Size: 2222 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4924
SphI G_CATG'C
Cuts at: 1132 2282 1132
Size: 1150 3774
SspI AAT'ATT
Cuts at: 5 52 2848 5
Size: 47 2796 2081
Fragments arranged by size:
2796 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4924
StyI C'CwwG_G
Cuts at: 513 1065 2363 513
Size: 552 1298 3074
Fragments arranged by size:
3074 1298 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2951 2968 3121 3306 3751 325
Size: 1255 1371 17 153 185 445 1498
Fragments arranged by size:
1498 1371 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3170 4882 96
Size: 274 80 33 51 1443 1193 1712 138
Fragments arranged by size:
1712 1443 1193 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4884 4916 4884
Size: 32 4892
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4924
XbaI T'CTAG_A
Cuts at: 2260 2260
Size: 4924
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4924
XmaI C'CCGG_G
Cuts at: 1870 2249 1870
Size: 379 4545
XmnI GAAnn'nnTTC
Cuts at: 3053 3053
Size: 4924
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2732 276
Size: 53 83 186 936 1198 2468
Fragments arranged by size:
2468 1198 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI