Restriction map for
pFN21AE0201
(Circular) (Six-base) MAPSORT of: pFN21AE0201.seq Check: 3514 from: 1 to: 4954
pFN21AE0201 4954 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2094 2764 278
Size: 53 83 186 936 558 670 2468
Fragments arranged by size:
2468 936 670 558 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2297 1937
Size: 360 4594
Acc65I G'GTAC_C
Cuts at: 2275 2275
Size: 4954
AclI AA'CG_TT
Cuts at: 1563 3081 3454 1563
Size: 1518 373 3063
Fragments arranged by size:
3063 1518 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 3017 4401 4669 1632
Size: 503 882 1384 268 1917
Fragments arranged by size:
1917 1384 882 503 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4757
AflIII A'CryG_T
Cuts at: 1203 3853 4830 1203
Size: 2650 977 1327
Fragments arranged by size:
2650 1327 977
AgeI A'CCGG_T
Cuts at: 3785 3785
Size: 4954
AhdI GACnn_n'nnGTC
Cuts at: 3683 3683
Size: 4954
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4954
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4954
AlwNI CAG_nnn'CTG
Cuts at: 2697 4269 2697
Size: 1572 3382
ApaLI G'TGCA_C
Cuts at: 3011 4167 3011
Size: 1156 3798
ApoI r'AATT_y
Cuts at: 1493 2263 2489 2641 4750 1493
Size: 770 226 152 2109 1697
Fragments arranged by size:
2109 1697 770 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3508 160
Size: 872 2476 1606
Fragments arranged by size:
2476 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4954
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 2279 1870
Size: 73 205 131 4545
Fragments arranged by size:
4545 205 131 73
BamHI G'GATC_C
Cuts at: 2284 2651 2284
Size: 367 4587
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2275 3730 618
Size: 325 217 627 488 1455 1842
Fragments arranged by size:
1842 1455 627 488 325 217
BanII G_rGCy'C
Cuts at: 1391 2273 1391
Size: 882 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3145 3179 1903
Size: 34 1208 34 3678
Fragments arranged by size:
3678 1208 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2846 4056 2846
Size: 1210 3744
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4954
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3565 136
Size: 107 122 71 631 605 1893 1525
Fragments arranged by size:
1893 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4949 4949
Size: 4954
BlpI GC'TnA_GC
Cuts at: 2371 2371
Size: 4954
Bme1580I G_kGCm'C
Cuts at: 1790 3015 4171 1790
Size: 1225 1156 2573
Fragments arranged by size:
2573 1225 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4954
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2685 3643 442
Size: 416 53 83 399 24 1268 958 1753
Fragments arranged by size:
1753 1268 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2156 3614 1239
Size: 841 76 1458 2579
Fragments arranged by size:
2579 1458 841 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 3079 3944 4242 2225
Size: 854 865 298 2937
Fragments arranged by size:
2937 865 854 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3617 882
Size: 617 2118 2219
Fragments arranged by size:
2219 2118 617
BsaAI yAC'GTr
Cuts at: 493 1204 2745 493
Size: 711 1541 2702
Fragments arranged by size:
2702 1541 711
BsaBI GATnn'nnATC
Cuts at: 4948 4948
Size: 4954
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2761 3143 3778
Size: 53 83 186 936 558 670 382 635
Cuts at: 3778 275
Size: 1451
Fragments arranged by size:
1451 936 670 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3387 3785 4059 4206 4617 3387
Size: 398 274 147 411 3724
Fragments arranged by size:
3724 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4729
Fragments arranged by size:
4729 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4954
BseYI C'CCAG_C
Cuts at: 2065 2071 4157 4653 2065
Size: 6 2086 496 2366
Fragments arranged by size:
2366 2086 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2404 3165 4193 1540
Size: 275 186 51 352 761 1028 2301
Fragments arranged by size:
2301 1028 761 352 275 186 51
BsiHKAI G_wGCw'C
Cuts at: 3015 3100 4171 3015
Size: 85 1071 3798
Fragments arranged by size:
3798 1071 85
BsmI GAATG_Cn'
Cuts at: 2465 2558 2465
Size: 93 4861
BsmBI CGTCTCn'nnnn_
Cuts at: 2098 2098
Size: 4954
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2273 3015 3100 4171 1391
Size: 399 483 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 483 399 85
BspEI T'CCGG_A
Cuts at: 4617 4617
Size: 4954
BspHI T'CATG_A
Cuts at: 2843 4573 2843
Size: 1730 3224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2309 844
Size: 1465 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2841 1394
Size: 429 1018 3507
Fragments arranged by size:
3507 1018 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3449 3623 65
Size: 1183 2201 174 1396
Fragments arranged by size:
2201 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3598 3785 1700
Size: 110 1788 187 2869
Fragments arranged by size:
2869 1788 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4954
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4954
BssSI C'ACGA_G
Cuts at: 3014 4026 3014
Size: 1012 3942
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4954
BstBI TT'CG_AA
Cuts at: 3758 3758
Size: 4954
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4954
BstXI CCAn_nnnn'nTGG
Cuts at: 2104 2104
Size: 4954
BstYI r'GATC_y
Cuts at: 1972 2284 2651 3036 3053 4505 4591 4603 4949
Size: 312 367 385 17 1452 86 12 346
Cuts at: 4949 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 312 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4215
Fragments arranged by size:
4215 683 56
BtsI GCAGTG_nn'
Cuts at: 2470 3264 3284 2470
Size: 794 20 4140
Fragments arranged by size:
4140 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4954
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4919
DraI TTT'AAA
Cuts at: 2258 2258
Size: 4954
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3961 809
Size: 507 2645 1802
Fragments arranged by size:
2645 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2401 3290 4919
Size: 54 1425 50 275 18 571 889 1629
Cuts at: 4919 8
Size: 43
Fragments arranged by size:
1629 1425 889 571 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2401 1537
Size: 275 589 4090
Fragments arranged by size:
4090 589 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2884 1353
Size: 665 866 3423
Fragments arranged by size:
3423 866 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3541 3915 4061 387
Size: 1064 138 1952 374 146 1280
Fragments arranged by size:
1952 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2135 2156 3017 3614 4401 4669
Size: 393 448 55 21 861 597 787 268
Cuts at: 4669 1239
Size: 1524
Fragments arranged by size:
1524 861 787 597 448 393 268 55
21
EcoO109I rG'GnC_Cy
Cuts at: 2082 2082
Size: 4954
EcoRI G'AATT_C
Cuts at: 2263 2641 2263
Size: 378 4576
FspI TGC'GCA
Cuts at: 3460 3460
Size: 4954
HaeII r_GCGC'y
Cuts at: 1678 4101 1678
Size: 2423 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3595 3627 3669 3701 1373
Size: 32 135 32 2023 32 42 32 2626
Fragments arranged by size:
2626 2023 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2298 2544 669
Size: 988 281 360 246 3079
Fragments arranged by size:
3079 988 360 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4954
HpaI GTT'AAC
Cuts at: 2544 2544
Size: 4954
KpnI G_GTAC'C
Cuts at: 2279 2279
Size: 4954
MfeI C'AATT_G
Cuts at: 2553 2553
Size: 4954
MluI A'CGCG_T
Cuts at: 4830 4830
Size: 4954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4068 4252 1305
Size: 2763 184 2007
Fragments arranged by size:
2763 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3132
Fragments arranged by size:
3132 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2912 3271 3430 518
Size: 648 1746 359 159 2042
Fragments arranged by size:
2042 1746 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2370 3047 4195 4440 1606
Size: 764 677 1148 245 2120
Fragments arranged by size:
2120 1148 764 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4402
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4954
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4954
NotI GC'GGCC_GC
Cuts at: 2401 2401
Size: 4954
NspI r_CATG'y
Cuts at: 1132 1229 2312 3857 1132
Size: 97 1083 1545 2229
Fragments arranged by size:
2229 1545 1083 97
PasI CC'CwG_GG
Cuts at: 1348 2077 1348
Size: 729 4225
PciI A'CATG_T
Cuts at: 3853 3853
Size: 4954
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4954
PmeI GTTT'AAAC
Cuts at: 2258 2258
Size: 4954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 4536 4568 3023
Size: 32 1481 32 3409
Fragments arranged by size:
3409 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2082 2082
Size: 4954
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4954
PsiI TTA'TAA
Cuts at: 2524 2524
Size: 4954
PstI C_TGCA'G
Cuts at: 830 2306 830
Size: 1476 3478
PvuI CG_AT'CG
Cuts at: 2001 2052 2001
Size: 51 4903
SalI G'TCGA_C
Cuts at: 1936 2296 1936
Size: 360 4594
SanDI GG'GwC_CC
Cuts at: 2082 2082
Size: 4954
SapI GCTCTTCn'nnn_
Cuts at: 2018 2018
Size: 4954
SbfI CC_TGCA'GG
Cuts at: 2306 2306
Size: 4954
ScaI AGT'ACT
Cuts at: 3202 4914 3202
Size: 1712 3242
SfcI C'TryA_G
Cuts at: 826 1045 2302 3437 4118 4309 826
Size: 219 1257 1135 681 191 1471
Fragments arranged by size:
1471 1257 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4954
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4954
SmaI CCC'GGG
Cuts at: 1872 2281 1872
Size: 409 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 3058 3959 4221 820
Size: 197 926 297 818 901 262 1553
Fragments arranged by size:
1553 926 901 818 297 262 197
SnaBI TAC'GTA
Cuts at: 493 2745 493
Size: 2252 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4954
SphI G_CATG'C
Cuts at: 1132 2312 1132
Size: 1180 3774
SspI AAT'ATT
Cuts at: 5 52 2878 5
Size: 47 2826 2081
Fragments arranged by size:
2826 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4954
StyI C'CwwG_G
Cuts at: 513 1065 2103 2393 513
Size: 552 1038 290 3074
Fragments arranged by size:
3074 1038 552 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2981 2998 3151 3336 3781 325
Size: 1255 1401 17 153 185 445 1498
Fragments arranged by size:
1498 1401 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3200 4912 96
Size: 274 80 33 51 1443 1223 1712 138
Fragments arranged by size:
1712 1443 1223 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4914 4946 4914
Size: 32 4922
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4954
XbaI T'CTAG_A
Cuts at: 2290 2290
Size: 4954
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4954
XmaI C'CCGG_G
Cuts at: 1870 2279 1870
Size: 409 4545
XmnI GAAnn'nnTTC
Cuts at: 3083 3083
Size: 4954
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2092 2762 276
Size: 53 83 186 936 558 670 2468
Fragments arranged by size:
2468 936 670 558 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI XcmI