################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I Cac8I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI Fnu4HI FokI FseI FspI HgaI Hin1I HindIII HinfI HpaI ItaI KasI KpnI KspI MaeI MaeII MaeIII MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I TseI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 249 CCGC CCGC(-3/-1) AfaI 51 GTAC GT^AC AluI 180 AGCT AG^CT AlwI 221 GGATC GGATC(4/5) AspHI 208 GTGCTC G[AT]GC[AT]^C BglII 236 AGATCT A^GATCT BlpI 176 GCTCAGC GC^T[ACGT]AGC BpmI 7 CTGGAG CTGGAG(16/14) Bpu1102I 176 GCTCAGC GC^T[ACGT]AGC BsaBI 222 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 25 GAGGAG GAGGAG(10/8) BsiHKAI 208 GTGCTC G[AT]GC[AT]^C BsrFI 242 ACCGGC [GA]^CCGG[CT] BstXI 284 CCATACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 236 AGATCT [GA]^GATC[CT] CelII 176 GCTCAGC GC^T[ACGT]AGC CfoI 174 GCGC GCG^C Cfr10I 242 ACCGGC [GA]^CCGG[CT] Cfr13I 245 GGCCC G^G[ACGT]CC DdeI 177 CTCAG C^T[ACGT]AG EarI 193 CTCTTC CTCTTC(1/4) EcoT14I 261 CCTTGG C^C[AT][AT]GG FauI 248 CCCGC CCCGC(4/6) HaeII 173 GGCGCT [GA]GCGC^[CT] HhaI 174 GCGC GCG^C HinP1I 174 GCGC G^CGC HincII 94 GTCAAC GT[CT]^[GA]AC HindII 94 GTCAAC GT[CT]^[GA]AC Ksp632I 193 CTCTTC CTCTTC(1/4) MamI 222 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 236 AGATCT [GA]^GATC[CT] PvuII 179 CAGCTG CAG^CTG RsaI 51 GTAC GT^AC Sau96I 245 GGCCC G^G[ACGT]CC ScaI 50 AGTACT AGT^ACT StuI 119 AGGCCT AGG^CCT StyI 261 CCTTGG C^C[AT][AT]GG Tsp509I 32 AATT ^AATT XhoII 236 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BanI 256 GGTGCC G^G[CT][GA]CC 295 GGCACC BsiYI 192 CCTCTTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 247 CCCCGCTGGGG BslI 192 CCTCTTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 247 CCCCGCTGGGG BsmFI 266 GGGAC GGGAC(10/14) 130 GTCCC DpnII 222 GATC ^GATC 237 GATC HaeIII 120 GGCC GG^CC 245 GGCC HapII 45 CCGG C^CGG 243 CCGG HpaII 45 CCGG C^CGG 243 CCGG MboI 222 GATC ^GATC 237 GATC MspA1I 179 CAGCTG C[AC]G^C[GT]G 249 CCGCTG MspI 45 CCGG C^CGG 243 CCGG NdeII 222 GATC ^GATC 237 GATC Sau3AI 222 GATC ^GATC 237 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### BmyI BsaJI Bsp1286I BssKI BstNI EcoRII HphI MboII MnlI MvaI NlaIV ScrFI