(Circular) (Six-base) MAPSORT of: pFN21AB5317.seq Check: 8432 from: 1 to: 5881 pFN21AB5317 5881 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2288 2932 3944 5328 5596 1632 Size: 656 644 1012 1384 268 1917 Fragments arranged by size: 1917 1384 1012 656 644 268 AfeI AGC'GCT Cuts at: 2945 2945 Size: 5881 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 2682 4780 5757 1203 Size: 1479 2098 977 1327 Fragments arranged by size: 2098 1479 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 2546 2764 3014 3028 3091 3624 5196 2546 Size: 218 250 14 63 533 1572 3231 Fragments arranged by size: 3231 1572 533 250 218 63 14 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 2146 2198 3190 3416 3568 5677 1493 Size: 653 52 992 226 152 2109 1697 Fragments arranged by size: 2109 1697 992 653 226 152 52 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2117 3206 1870 Size: 73 174 1089 4545 Fragments arranged by size: 4545 1089 174 73 BamHI G'GATC_C Cuts at: 2371 3211 3578 2371 Size: 840 367 4674 Fragments arranged by size: 4674 840 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2726 3133 3202 4657 618 Size: 325 217 627 939 407 69 1455 1842 Fragments arranged by size: 1842 1455 939 627 407 325 217 69 BanII G_rGCy'C Cuts at: 1391 3200 1391 Size: 1809 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2367 3084 928 Size: 971 468 717 3725 Fragments arranged by size: 3725 971 717 468 BbvCI CC'TCA_GC Cuts at: 2675 2675 Size: 5881 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2040 2074 4072 4106 1903 Size: 34 103 34 1998 34 3678 Fragments arranged by size: 3678 1998 103 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4492 136 Size: 107 122 71 631 605 337 2483 1525 Fragments arranged by size: 2483 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 2894 3298 2894 Size: 404 5477 Bme1580I G_kGCm'C Cuts at: 1790 3942 5098 1790 Size: 2152 1156 2573 Fragments arranged by size: 2573 2152 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2080 3612 4570 Size: 416 53 83 399 24 663 1532 958 Cuts at: 4570 442 Size: 1753 Fragments arranged by size: 1753 1532 958 663 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2143 2143 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2386 2804 4541 1239 Size: 1147 418 1737 2579 Fragments arranged by size: 2579 1737 1147 418 Bpu10I CC'TnA_GC Cuts at: 2675 2675 Size: 5881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2162 2924 4006 4871 5169 2162 Size: 762 1082 865 298 2874 Fragments arranged by size: 2874 1082 865 762 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 4544 882 Size: 617 792 2253 2219 Fragments arranged by size: 2253 2219 792 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 5875 5875 Size: 5881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2465 4314 4712 4986 5133 5544 2465 Size: 1849 398 274 147 411 2802 Fragments arranged by size: 2802 1849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5656 Fragments arranged by size: 5656 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2303 2306 2309 2327 2492 2525 2573 Size: 904 213 3 3 18 165 33 48 Cuts at: 2573 2598 2762 2995 3054 1186 Size: 25 164 233 59 4013 Fragments arranged by size: 4013 904 233 213 165 164 59 48 33 25 18 3 3 BseYI C'CCAG_C Cuts at: 5084 5580 5084 Size: 496 5385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 1163 Size: 573 1041 4267 Fragments arranged by size: 4267 1041 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3171 3942 4027 5098 3171 Size: 771 85 1071 3954 Fragments arranged by size: 3954 1071 771 85 BsmI GAATG_Cn' Cuts at: 3392 3485 3392 Size: 93 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3171 3200 3942 4027 5098 1391 Size: 399 1381 29 742 85 1071 2174 Fragments arranged by size: 2174 1381 1071 742 399 85 29 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3090 3236 844 Size: 2246 146 3489 Fragments arranged by size: 3489 2246 146 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4376 4550 65 Size: 1183 3128 174 1396 Fragments arranged by size: 3128 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4525 4712 1700 Size: 110 2715 187 2869 Fragments arranged by size: 2869 2715 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 3941 4953 3941 Size: 1012 4869 BstAPI GCAn_nnn'nTGC Cuts at: 794 2626 794 Size: 1832 4049 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstXI CCAn_nnnn'nTGG Cuts at: 2999 2999 Size: 5881 BstYI r'GATC_y Cuts at: 1972 2280 2371 2415 3211 3578 3963 3980 5432 Size: 308 91 44 796 367 385 17 1452 Cuts at: 5432 5518 5530 5876 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 796 385 367 346 308 91 86 44 17 12 Bsu36I CC'TnA_GG Cuts at: 2547 3007 2547 Size: 460 5421 BtgI C'CryG_G Cuts at: 513 1065 2004 2178 513 Size: 552 939 174 4216 Fragments arranged by size: 4216 939 552 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 3397 4191 4211 3397 Size: 794 20 5067 Fragments arranged by size: 5067 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3162 3185 3162 Size: 23 5858 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846 Size: 54 1425 50 275 18 1498 889 1629 Cuts at: 5846 8 Size: 43 Fragments arranged by size: 1629 1498 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2424 2430 2444 2550 2568 3811 1353 Size: 675 396 6 14 106 18 1243 3423 Fragments arranged by size: 3423 1243 675 396 106 18 14 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2893 2978 4468 4842 4988 387 Size: 1064 138 1304 85 1490 374 146 1280 Fragments arranged by size: 1490 1304 1280 1064 374 146 138 85 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2386 2804 2932 3944 4541 5328 Size: 393 656 98 418 128 1012 597 787 Cuts at: 5328 5596 1239 Size: 268 1524 Fragments arranged by size: 1524 1012 787 656 597 418 393 268 128 98 EcoNI CCTnn'n_nnAGG Cuts at: 2618 2618 Size: 5881 EcoO109I rG'GnC_Cy Cuts at: 2011 2044 2068 2122 2185 2011 Size: 33 24 54 63 5707 Fragments arranged by size: 5707 63 54 33 24 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2827 2827 Size: 5881 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 2947 5028 1678 Size: 1269 2081 2531 Fragments arranged by size: 2531 2081 1269 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4522 4554 4596 4628 1373 Size: 32 135 32 2950 32 42 32 2626 Fragments arranged by size: 2950 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5881 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2991 4995 5179 1305 Size: 1686 2004 184 2007 Fragments arranged by size: 2007 2004 1686 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3839 4198 4357 518 Size: 648 2673 359 159 2042 Fragments arranged by size: 2673 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2764 3297 3974 5122 5367 1606 Size: 1158 533 677 1148 245 2120 Fragments arranged by size: 2120 1158 1148 677 533 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2178 513 Size: 552 939 174 4216 Fragments arranged by size: 4216 939 552 174 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 2686 3239 4784 1132 Size: 97 1457 553 1545 2229 Fragments arranged by size: 2229 1545 1457 553 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 2682 4780 2682 Size: 2098 3783 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2423 2455 3950 3982 5463 5495 2423 Size: 32 1495 32 1481 32 2809 Fragments arranged by size: 2809 1495 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2044 2068 2122 2185 2044 Size: 24 54 63 5740 Fragments arranged by size: 5740 63 54 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PstI C_TGCA'G Cuts at: 830 2622 2904 2979 3087 3233 830 Size: 1792 282 75 108 146 3478 Fragments arranged by size: 3478 1792 282 146 108 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2764 2764 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SbfI CC_TGCA'GG Cuts at: 2622 3233 2622 Size: 611 5270 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SexAI A'CCwGG_T Cuts at: 2715 2715 Size: 5881 SfcI C'TryA_G Cuts at: 826 1045 2618 2900 2975 3083 3229 4364 5045 Size: 219 1573 282 75 108 146 1135 681 Cuts at: 5045 5236 826 Size: 191 1471 Fragments arranged by size: 1573 1471 1135 681 282 219 191 146 108 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 2141 2939 3985 4886 5148 Size: 197 926 174 24 798 1046 901 262 Cuts at: 5148 820 Size: 1553 Fragments arranged by size: 1553 1046 926 901 798 262 197 174 24 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2004 2178 3320 513 Size: 552 939 174 1142 3074 Fragments arranged by size: 3074 1142 939 552 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2261 2599 2717 3908 3925 4078 4263 Size: 1255 681 338 118 1191 17 153 185 Cuts at: 4263 4708 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1191 681 445 338 185 153 118 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4127 5839 96 Size: 274 80 33 51 1443 2150 1712 138 Fragments arranged by size: 2150 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3025 3057 5841 5873 3025 Size: 32 2784 32 3033 Fragments arranged by size: 3033 2784 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2609 1316 Size: 1293 4588 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2093 2093 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 2117 1943 Size: 174 5707 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 4010 4010 Size: 5881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI