Restriction map for
pFN21AB5317
(Linear) (Six-base) MAPSORT of: KIBB5317.seq Check: 7195 from: 1 to: 1176
KIBB5317.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 927 1176
Size: 283 644 249
Fragments arranged by size:
644 283 249
AfeI AGC'GCT
Cuts at: 0 940 1176
Size: 940 236
AflIII A'CryG_T
Cuts at: 0 677 1176
Size: 677 499
AlwNI CAG_nnn'CTG
Cuts at: 0 541 759 1009 1023 1086 1176
Size: 541 218 250 14 63 90
Fragments arranged by size:
541 250 218 90 63 14
ApoI r'AATT_y
Cuts at: 0 141 193 1176
Size: 141 52 983
Fragments arranged by size:
983 141 52
AvaI C'yCGr_G
Cuts at: 0 112 1176
Size: 112 1064
BamHI G'GATC_C
Cuts at: 0 366 1176
Size: 366 810
BanI G'GyrC_C
Cuts at: 0 721 1128 1176
Size: 721 407 48
Fragments arranged by size:
721 407 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 362 1079 1176
Size: 362 717 97
Fragments arranged by size:
717 362 97
BbvCI CC'TCA_GC
Cuts at: 0 670 1176
Size: 670 506
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 35 69 1176
Size: 35 34 1107
Fragments arranged by size:
1107 35 34
BlpI GC'TnA_GC
Cuts at: 0 889 1176
Size: 889 287
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 1176
Size: 75 1101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 1176
Size: 138 1038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 799 1176
Size: 381 418 377
Fragments arranged by size:
418 381 377
Bpu10I CC'TnA_GC
Cuts at: 0 670 1176
Size: 670 506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 919 1176
Size: 157 762 257
Fragments arranged by size:
762 257 157
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 1176
Size: 286 890
BsaWI w'CCGG_w
Cuts at: 0 460 1176
Size: 460 716
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 298 301 304 322 487 520 568
Size: 85 213 3 3 18 165 33 48
Cuts at: 568 593 757 990 1049 1176
Size: 25 164 233 59 127
Fragments arranged by size:
233 213 165 164 127 85 59 48
33 25 18 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1176
Size: 772 404
BsiHKAI G_wGCw'C
Cuts at: 0 1166 1176
Size: 1166 10
Bsp1286I G_dGCh'C
Cuts at: 0 1166 1176
Size: 1166 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1085 1176
Size: 1085 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 621 1176
Size: 621 555
BstXI CCAn_nnnn'nTGG
Cuts at: 0 994 1176
Size: 994 182
BstYI r'GATC_y
Cuts at: 0 275 366 410 1176
Size: 275 91 44 766
Fragments arranged by size:
766 275 91 44
Bsu36I CC'TnA_GG
Cuts at: 0 542 1002 1176
Size: 542 460 174
Fragments arranged by size:
542 460 174
BtgI C'CryG_G
Cuts at: 0 173 1176
Size: 173 1003
DraI TTT'AAA
Cuts at: 0 1157 1176
Size: 1157 19
EarI CTCTTCn'nnn_
Cuts at: 0 23 419 425 439 545 563 1176
Size: 23 396 6 14 106 18 613
Fragments arranged by size:
613 396 106 23 18 14 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 888 973 1176
Size: 888 85 203
Fragments arranged by size:
888 203 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 381 799 927 1176
Size: 283 98 418 128 249
Fragments arranged by size:
418 283 249 128 98
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 1176
Size: 613 563
EcoO109I rG'GnC_Cy
Cuts at: 0 6 39 63 117 180 1176
Size: 6 33 24 54 63 996
Fragments arranged by size:
996 63 54 33 24 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 822 1176
Size: 822 354
HaeII r_GCGC'y
Cuts at: 0 942 1176
Size: 942 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1176
Size: 986 190
MspA1I CmG'CkG
Cuts at: 0 759 1176
Size: 759 417
NcoI C'CATG_G
Cuts at: 0 173 1176
Size: 173 1003
NspI r_CATG'y
Cuts at: 0 681 1176
Size: 681 495
PciI A'CATG_T
Cuts at: 0 677 1176
Size: 677 499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 1176
Size: 418 32 726
Fragments arranged by size:
726 418 32
PpuMI rG'GwC_Cy
Cuts at: 0 39 63 117 180 1176
Size: 39 24 54 63 996
Fragments arranged by size:
996 63 54 39 24
PstI C_TGCA'G
Cuts at: 0 617 899 974 1082 1176
Size: 617 282 75 108 94
Fragments arranged by size:
617 282 108 94 75
PvuII CAG'CTG
Cuts at: 0 759 1176
Size: 759 417
SbfI CC_TGCA'GG
Cuts at: 0 617 1176
Size: 617 559
SexAI A'CCwGG_T
Cuts at: 0 710 1176
Size: 710 466
SfcI C'TryA_G
Cuts at: 0 613 895 970 1078 1176
Size: 613 282 75 108 98
Fragments arranged by size:
613 282 108 98 75
SmlI C'TyrA_G
Cuts at: 0 112 136 934 1176
Size: 112 24 798 242
Fragments arranged by size:
798 242 112 24
StyI C'CwwG_G
Cuts at: 0 173 1176
Size: 173 1003
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 594 712 1176
Size: 256 338 118 464
Fragments arranged by size:
464 338 256 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1020 1052 1176
Size: 1020 32 124
Fragments arranged by size:
1020 124 32
Tth111I GACn'n_nGTC
Cuts at: 0 604 1176
Size: 604 572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 88 1176
Size: 88 1088
XhoI C'TCGA_G
Cuts at: 0 112 1176
Size: 112 1064
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApoI AvaI BamHI BanI
BbsI BbvCI BcgI BlpI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspMI
BstAPI BstXI BstYI Bsu36I BtgI DraI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII MmeI MspA1I NcoI
NspI PciI PpiI PpuMI PstI PvuII SbfI SexAI
SfcI SmlI StyI TaqII TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanII BbeI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmtI BsaAI BsaBI BsaHI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XbaI XmaI XmnI ZraI