Restriction map for
pFN21AB7986
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII AccII AciI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI ApoI AscI Asp700I
AspEI AspI AvaII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI BsmI BspDI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 764 GGTACC G^GTACC
AccI 869 GTCTAC GT^[AC][GT]AC
AccIII 164 TCCGGA T^CCGGA
Alw44I 968 GTGCAC G^TGCAC
AlwNI 529 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 700 CCTGAGG CC^T[ACGT]AGG
ApaLI 968 GTGCAC G^TGCAC
Asp718I 764 GGTACC G^GTACC
AspHI 968 GTGCAC G[AT]GC[AT]^C
AvaI 63 CCCGGG C^[CT]CG[GA]G
AviII 17 TGCGCA TGC^GCA
BanI 764 GGTACC G^G[CT][GA]CC
BanII 67 GGGCTC G[GA]GC[CT]^C
BbsI 880 GTCTTC GAAGAC(2/6)
BfaI 34 CTAG C^TAG
BglII 888 AGATCT A^GATCT
BlpI 308 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 308 GCTCAGC GC^T[ACGT]AGC
BpuAI 880 GTCTTC GAAGAC(2/6)
BsaI 24 GAGACC GGTCTC(1/5)
BseAI 164 TCCGGA T^CCGGA
BsiHKAI 968 GTGCAC G[AT]GC[AT]^C
BsmFI 137 GGGAC GGGAC(10/14)
BsoBI 63 CCCGGG C^[CT]CG[GA]G
Bsp1407I 626 TGTACA T^GTACA
BspEI 164 TCCGGA T^CCGGA
BspHI 710 TCATGA T^CATGA
BsrGI 626 TGTACA T^GTACA
BstXI 908 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 700 CCTGAGG CC^T[ACGT]AGG
CelII 308 GCTCAGC GC^T[ACGT]AGC
CfoI 18 GCGC GCG^C
DraII 530 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 109 CCATGG C^C[GA][CT]GG
Eco81I 700 CCTGAGG CC^T[ACGT]AGG
EcoO109I 530 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 268 GATATC GAT^ATC
EcoT14I 109 CCATGG C^C[AT][AT]GG
FspI 17 TGCGCA TGC^GCA
HhaI 18 GCGC GCG^C
HinP1I 18 GCGC G^CGC
KpnI 764 GGTACC GGTAC^C
MaeI 34 CTAG C^TAG
MaeIII 642 GTCAC ^GT[ACGT]AC
MroI 164 TCCGGA T^CCGGA
MspA1I 311 CAGCTG C[AC]G^C[GT]G
NcoI 109 CCATGG C^CATGG
PflMI 221 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 930 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 311 CAGCTG CAG^CTG
RcaI 710 TCATGA T^CATGA
SfcI 871 CTACAG C^T[GA][CT]AG
SmaI 63 CCCGGG CCC^GGG
SphI 117 GCATGC GCATG^C
SspBI 626 TGTACA T^GTACA
StyI 109 CCATGG C^C[AT][AT]GG
TfiI 424 GATTC G^A[AT]TC
Tsp45I 642 GTCAC ^GT[GC]AC
Van91I 221 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 63 CCCGGG C^CCGGG
XmaI 63 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 105 GATCC GGATC(4/5)
217 GATCC
AseI 46 ATTAAT AT^TAAT
79 ATTAAT
AsnI 46 ATTAAT AT^TAAT
79 ATTAAT
BcnI 63 CCCGG CC^[GC]GG
64 CCGGG
BmyI 67 GGGCTC G[AGT]GC[ACT]^C
968 GTGCAC
BpmI 539 CTCCAG CTGGAG(16/14)
782 CTGGAG
BsmAI 24 GAGAC GTCTC(1/5)
809 GAGAC
Bsp1286I 67 GGGCTC G[AGT]GC[ACT]^C
968 GTGCAC
Cfr13I 173 GGGCC G^G[ACGT]CC
531 GGCCC
MaeII 226 ACGT A^CGT
749 ACGT
MseI 47 TTAA T^TAA
80 TTAA
MslI 9 CACTCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
588 CATCCCAGTG
MwoI 33 GCTAGAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
717 GCTCTTGGAGC
NciI 63 CCCGG CC^[GC]GG
64 CCGGG
NspI 117 GCATGC [GA]CATG^[CT]
506 ACATGC
PshBI 46 ATTAAT AT^TAAT
79 ATTAAT
Sau96I 173 GGGCC G^G[ACGT]CC
531 GGCCC
TaqI 272 TCGA T^CGA
373 TCGA
Tru9I 47 TTAA T^TAA
80 TTAA
TthHB8I 272 TCGA T^CGA
373 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsaWI
BseRI BsiYI BslI BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MflI MnlI MspI
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI ScrFI SfaNI TseI
Tsp509I TspRI XhoII