################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 57 GTAC GT^AC AocI 256 CCTGAGG CC^T[ACGT]AGG AvaII 397 GGACC G^G[AT]CC BbvI 216 GCTGC GCAGC(8/12) BsaI 261 GGTCTC GGTCTC(1/5) BsmFI 396 GGGAC GGGAC(10/14) BspMI 151 GCAGGT ACCTGC(4/8) BsrI 193 CCAGT ACTGG(1/-1) BstF5I 374 GGATG GGATG(2/0) BstNI 69 CCTGG CC^[AT]GG BstYI 294 AGATCC [GA]^GATC[CT] Bsu36I 256 CCTGAGG CC^T[ACGT]AGG DraII 396 GGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 256 CCTGAGG CC^T[ACGT]AGG EcoO109I 396 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 179 GAATTC G^AATTC EcoRII 69 CCTGG ^CC[AT]GG EcoT14I 269 CCTTGG C^C[AT][AT]GG FokI 374 GGATG GGATG(9/13) HapII 39 CCGG C^CGG HindIII 319 AAGCTT A^AGCTT HpaII 39 CCGG C^CGG MboII 27 GAAGA GAAGA(8/7) MflI 294 AGATCC [GA]^GATC[CT] MspI 39 CCGG C^CGG MvaI 69 CCTGG CC^[AT]GG MwoI 5 GCCGCGTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 396 GGGACC GG[ACGT]^[ACGT]CC PpuMI 396 GGGACCT [GA]G^G[AT]CC[CT] PstI 217 CTGCAG CTGCA^G RsaI 57 GTAC GT^AC ScaI 56 AGTACT AGT^ACT SfaNI 165 GCATC GCATC(5/9) SmaI 38 CCCGGG CCC^GGG StyI 269 CCTTGG C^C[AT][AT]GG TseI 216 GCTGC G^C[AT]GC XhoII 294 AGATCC [GA]^GATC[CT] XmaCI 38 CCCGGG C^CCGGG XmaI 38 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 179 GAATTC [GA]^AATT[CT] 378 GAATTT AlwI 295 GATCC GGATC(4/5) 273 GGATC ApoI 179 GAATTC [GA]^AATT[CT] 378 GAATTT AvaI 38 CCCGGG C^[CT]CG[GA]G 392 CTCGGG BcnI 38 CCCGG CC^[GC]GG 39 CCGGG BsiYI 193 CCAGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 364 CCTACAGTTGG BslI 193 CCAGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 364 CCTACAGTTGG BsmAI 242 GAGAC GTCTC(1/5) 262 GTCTC BsoBI 38 CCCGGG C^[CT]CG[GA]G 392 CTCGGG CfoI 66 GCGC GCG^C 96 GCGC HhaI 66 GCGC GCG^C 96 GCGC HinP1I 66 GCGC G^CGC 96 GCGC MaeIII 160 GTCAC ^GT[ACGT]AC 355 GTCAC NciI 38 CCCGG CC^[GC]GG 39 CCGGG SfcI 217 CTGCAG C^T[GA][CT]AG 365 CTACAG Tsp45I 160 GTCAC ^GT[GC]AC 355 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AccII AciI AluI BsaJI BseRI BssKI BstUI Cfr13I DdeI DpnII Fnu4HI HaeIII ItaI MboI MnlI MvnI NdeII Sau3AI Sau96I ScrFI Tsp509I