Restriction map for pFN21AE6138
 (Circular) (Six-base) MAPSORT of: pFN21AE6138.seq  Check: 9294  from: 1  to: 5110



pFN21AE6138  5110 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:53  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2920     278 
   Size:         53      83     186     936    1384    2468

  Fragments arranged by size: 

                2468    1384     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2453    1937 
   Size:        516    4594
 
Acc65I G'GTAC_C

Cuts at:   2431    2431 
   Size:       5110
 
AclI AA'CG_TT

Cuts at:   1563    3237    3610    1563 
   Size:       1674     373    3063

  Fragments arranged by size: 

                3063    1674     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2339    2342    3173    4557    4825    1632 
   Size:        707       3     831    1384     268    1917

  Fragments arranged by size: 

                1917    1384     831     707     268       3
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4913
 
AflIII A'CryG_T

Cuts at:   1203    4009    4986    1203 
   Size:       2806     977    1327

  Fragments arranged by size: 

                2806    1327     977
 
AgeI A'CCGG_T

Cuts at:   3941    3941 
   Size:       5110
 
AhdI GACnn_n'nnGTC

Cuts at:   3839    3839 
   Size:       5110
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5110
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5110
 
AlwNI CAG_nnn'CTG

Cuts at:   2853    4425    2853 
   Size:       1572    3538
 
ApaLI G'TGCA_C

Cuts at:   3167    4323    3167 
   Size:       1156    3954
 
ApoI r'AATT_y

Cuts at:   1493    2184    2383    2419    2645    2797    4906    1493 
   Size:        691     199      36     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     691     226     199     152      36
 
AseI AT'TA_AT

Cuts at:    160    1032    3664     160 
   Size:        872    2632    1606

  Fragments arranged by size: 

                2632    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5110
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2043    2397    2435    1870 
   Size:         73     100     354      38    4545

  Fragments arranged by size: 

                4545     354     100      73      38
 
BamHI G'GATC_C

Cuts at:   2440    2807    2440 
   Size:        367    4743
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2431    3886     618 
   Size:        325     217     627     644    1455    1842

  Fragments arranged by size: 

                1842    1455     644     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2429    1391 
   Size:       1038    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4139
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3301    3335    1903 
   Size:         34    1364      34    3678

  Fragments arranged by size: 

                3678    1364      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3002    4212    3002 
   Size:       1210    3900
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5110
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3721     136 
   Size:        107     122      71     631     605    2049    1525

  Fragments arranged by size: 

                2049    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5105    5105 
   Size:       5110
 
BlpI GC'TnA_GC

Cuts at:   2527    2527 
   Size:       5110
 
Bme1580I G_kGCm'C

Cuts at:   1790    3171    4327    1790 
   Size:       1381    1156    2573

  Fragments arranged by size: 

                2573    1381    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5110
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2192    2841    3799 
   Size:        416      53      83     399      24     775     649     958

Cuts at:   3799     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     775     649     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5110
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3770    1239 
   Size:       2531    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3235    4100    4398    3235 
   Size:        865     298    3947

  Fragments arranged by size: 

                3947     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2272    3773     882 
   Size:        617     773    1501    2219

  Fragments arranged by size: 

                2219    1501     773     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2901     493 
   Size:        711    1697    2702

  Fragments arranged by size: 

                2702    1697     711
 
BsaBI GATnn'nnATC

Cuts at:   5104    5104 
   Size:       5110
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2917    3299    3934     275 
   Size:         53      83     186     936    1384     382     635    1451

  Fragments arranged by size: 

                1451    1384     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3543    3941    4215    4362    4773    3543 
   Size:        398     274     147     411    3880

  Fragments arranged by size: 

                3880     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4885

  Fragments arranged by size: 

                4885     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2126    2243    2246    1186 
   Size:        940     117       3    4050

  Fragments arranged by size: 

                4050     940     117       3
 
BseYI C'CCAG_C

Cuts at:   2129    4313    4809    2129 
   Size:       2184     496    2430

  Fragments arranged by size: 

                2430    2184     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4537
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2560    3321    4349    1540 
   Size:        275     186     559     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     559     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3171    3256    4327    3171 
   Size:         85    1071    3954

  Fragments arranged by size: 

                3954    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2621    2714    2621 
   Size:         93    5017
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2429    3171    3256    4327    1391 
   Size:        399     639     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     639     399      85
 
BspEI T'CCGG_A

Cuts at:   4773    4773 
   Size:       5110
 
BspHI T'CATG_A

Cuts at:   2999    4729    2999 
   Size:       1730    3380
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2147    2465     844 
   Size:       1303     318    3489

  Fragments arranged by size: 

                3489    1303     318
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2997    1394 
   Size:        429    1174    3507

  Fragments arranged by size: 

                3507    1174     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3605    3779      65 
   Size:       1183    2357     174    1396

  Fragments arranged by size: 

                2357    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3754    3941    1700 
   Size:        110    1944     187    2869

  Fragments arranged by size: 

                2869    1944     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5110
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5110
 
BssSI C'ACGA_G

Cuts at:   3170    4182    3170 
   Size:       1012    4098
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5110
 
BstBI TT'CG_AA

Cuts at:   3914    3914 
   Size:       5110
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5110
 
BstYI r'GATC_y

Cuts at:   1972    2299    2440    2807    3192    3209    4661    4747    4759 
   Size:        327     141     367     385      17    1452      86      12

Cuts at:   4759    5105    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     327     141      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2262    2262 
   Size:       5110
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3619

  Fragments arranged by size: 

                3619     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4371

  Fragments arranged by size: 

                4371     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2626    3420    3440    2626 
   Size:        794      20    4296

  Fragments arranged by size: 

                4296     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5110
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5075
 
DraI TTT'AAA

Cuts at:   2414    2414 
   Size:       5110
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4117     809 
   Size:        507    2801    1802

  Fragments arranged by size: 

                2801    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2557    3446    5075 
   Size:         54    1425      50     275      18     727     889    1629

Cuts at:   5075       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     727     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2557    1537 
   Size:        275     745    4090

  Fragments arranged by size: 

                4090     745     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3040    1353 
   Size:       1687    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3697    4071    4217     387 
   Size:       1064     138    2108     374     146    1280

  Fragments arranged by size: 

                2108    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2339    2342    3173    3770    4557    4825    1239 
   Size:        393     707       3     831     597     787     268    1524

  Fragments arranged by size: 

                1524     831     787     707     597     393     268       3
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2402    2402 
   Size:       5110
 
EcoRI G'AATT_C

Cuts at:   2184    2419    2797    2184 
   Size:        235     378    4497

  Fragments arranged by size: 

                4497     378     235
 
FspI TGC'GCA

Cuts at:   3616    3616 
   Size:       5110
 
HaeII r_GCGC'y

Cuts at:   1678    4257    1678 
   Size:       2579    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3751    3783    3825    3857    1373 
   Size:         32     135      32    2179      32      42      32    2626

  Fragments arranged by size: 

                2626    2179     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2454    2700     669 
   Size:        988     281     516     246    3079

  Fragments arranged by size: 

                3079     988     516     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2324     748 
   Size:       1576    3534
 
HpaI GTT'AAC

Cuts at:   2700    2700 
   Size:       5110
 
KpnI G_GTAC'C

Cuts at:   2435    2435 
   Size:       5110
 
MfeI C'AATT_G

Cuts at:   2709    2709 
   Size:       5110
 
MluI A'CGCG_T

Cuts at:   4986    4986 
   Size:       5110
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2373    4224    4408    1305 
   Size:       1068    1851     184    2007

  Fragments arranged by size: 

                2007    1851    1068     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3288

  Fragments arranged by size: 

                3288    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3068    3427    3586     518 
   Size:        648    1902     359     159    2042

  Fragments arranged by size: 

                2042    1902     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2526    3203    4351    4596    1606 
   Size:        920     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     920     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3619

  Fragments arranged by size: 

                3619     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5110
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5110
 
NotI GC'GGCC_GC

Cuts at:   2557    2557 
   Size:       5110
 
NspI r_CATG'y

Cuts at:   1132    1229    2468    4013    1132 
   Size:         97    1239    1545    2229

  Fragments arranged by size: 

                2229    1545    1239      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5110
 
PciI A'CATG_T

Cuts at:   4009    4009 
   Size:       5110
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5110
 
PmeI GTTT'AAAC

Cuts at:   2414    2414 
   Size:       5110
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3179    3211    4692    4724    3179 
   Size:         32    1481      32    3565

  Fragments arranged by size: 

                3565    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2402    2402 
   Size:       5110
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5110
 
PsiI TTA'TAA

Cuts at:   2680    2680 
   Size:       5110
 
PstI C_TGCA'G

Cuts at:    830    2226    2462     830 
   Size:       1396     236    3478

  Fragments arranged by size: 

                3478    1396     236
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5110
 
SalI G'TCGA_C

Cuts at:   1936    2452    1936 
   Size:        516    4594
 
SbfI CC_TGCA'GG

Cuts at:   2462    2462 
   Size:       5110
 
ScaI AGT'ACT

Cuts at:   2063    3358    5070    2063 
   Size:       1295    1712    2103

  Fragments arranged by size: 

                2103    1712    1295
 
SfcI C'TryA_G

Cuts at:    826    1045    2222    2370    2458    3593    4274    4465     826 
   Size:        219    1177     148      88    1135     681     191    1471

  Fragments arranged by size: 

                1471    1177    1135     681     219     191     148      88
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5110
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5110
 
SmaI CCC'GGG

Cuts at:   1872    2045    2437    1872 
   Size:        173     392    4545

  Fragments arranged by size: 

                4545     392     173
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3214    4115    4377     820 
   Size:        197     926    1271     901     262    1553

  Fragments arranged by size: 

                1553    1271     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2901     493 
   Size:       2408    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5110
 
SphI G_CATG'C

Cuts at:   1132    2468    1132 
   Size:       1336    3774
 
SspI AAT'ATT

Cuts at:      5      52    3034       5 
   Size:         47    2982    2081

  Fragments arranged by size: 

                2982    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5110
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2274    2549     513 
   Size:        552     939     270     275    3074

  Fragments arranged by size: 

                3074     939     552     275     270
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3137    3154    3307    3492    3937     325 
   Size:       1255    1557      17     153     185     445    1498

  Fragments arranged by size: 

                1557    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2061    3356    5068 
   Size:        274      80      33      51    1443      84    1295    1712

Cuts at:   5068      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1295     274     138      84      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5070    5102    5070 
   Size:         32    5078
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5110
 
XbaI T'CTAG_A

Cuts at:   2446    2446 
   Size:       5110
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5110
 
XmaI C'CCGG_G

Cuts at:   1870    2043    2435    1870 
   Size:        173     392    4545

  Fragments arranged by size: 

                4545     392     173
 
XmnI GAAnn'nnTTC

Cuts at:   3239    3239 
   Size:       5110
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2918     276 
   Size:         53      83     186     936    1384    2468

  Fragments arranged by size: 

                2468    1384     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   DraIII  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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