################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII AccI AccII AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII BbrPI BcgI BciVI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FseI FspI HgaI HpaI KspI MaeII MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### Acc65I 508 GGTACC G^GTACC AcyI 2173 GGCGCC G[GA]^CG[CT]C AfaI 509 GTAC GT^AC AflII 1360 CTTAAG C^TTAAG Asp718I 508 GGTACC G^GTACC AspHI 1064 GAGCTC G[AT]GC[AT]^C AvaI 62 CCCGAG C^[CT]CG[GA]G AvrII 1881 CCTAGG C^CTAGG BalI 2081 TGGCCA TGG^CCA BanII 1064 GAGCTC G[GA]GC[CT]^C BbeI 2173 GGCGCC GGCGC^C BbvCI 27 CCTCAGC CCTCAGC(-5/-2) BclI 246 TGATCA T^GATCA BcnI 12 CCGGG CC^[GC]GG BfrI 1360 CTTAAG C^TTAAG BglI 7 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1881 CCTAGG C^CTAGG BmyI 1064 GAGCTC G[AGT]GC[ACT]^C BsaBI 18 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2173 GGCGCC G[GA]^CG[CT]C BsaI 1585 GGTCTC GGTCTC(1/5) BseRI 440 GAGGAG GAGGAG(10/8) BsiEI 1538 CGACCG CG[GA][CT]^CG BsiHKAI 1064 GAGCTC G[AT]GC[AT]^C BsmI 617 GCATTC GAATGC(1/-1) BsoBI 62 CCCGAG C^[CT]CG[GA]G Bsp1286I 1064 GAGCTC G[AGT]GC[ACT]^C BstAPI 1365 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 155 TTCGAA TT^CGAA DraIII 1366 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2081 TGGCCA [CT]^GGCC[GA] EarI 1929 GAAGAG CTCTTC(1/4) EcoNI 2064 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1944 GAATTC G^AATTC EcoRV 307 GATATC GAT^ATC FauI 2130 CCCGC CCCGC(4/6) FbaI 246 TGATCA T^GATCA HaeII 2173 GGCGCC [GA]GCGC^[CT] Hin1I 2173 GGCGCC G[GA]^CG[CT]C HindIII 1728 AAGCTT A^AGCTT KasI 2173 GGCGCC G^GCGCC KpnI 508 GGTACC GGTAC^C Ksp632I 1929 GAAGAG CTCTTC(1/4) MamI 18 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2081 TGGCCA TGG^CCA MscI 2081 TGGCCA TGG^CCA NarI 2173 GGCGCC GG^CGCC NciI 12 CCGGG CC^[GC]GG NcoI 1029 CCATGG C^CATGG NheI 2247 GCTAGC G^CTAGC NspV 155 TTCGAA TT^CGAA PflMI 657 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1351 GACCTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2090 CTGCAG CTGCA^G RsaI 509 GTAC GT^AC SacI 1064 GAGCTC GAGCT^C SexAI 1642 ACCAGGT A^CC[AT]GGT SfoI 2173 GGCGCC GGC^GCC SfuI 155 TTCGAA TT^CGAA SphI 1487 GCATGC GCATG^C TaqI 156 TCGA T^CGA Tsp45I 2032 GTGAC ^GT[GC]AC TthHB8I 156 TCGA T^CGA Van91I 657 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 117 CCATATTAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 314 ACATGT A^C[GA][CT]GT 1791 ACATGT BamHI 50 GGATCC G^GATCC 427 GGATCC BbsI 2011 GAAGAC GAAGAC(2/6) 93 GTCTTC BglII 764 AGATCT A^GATCT 1803 AGATCT BpuAI 2011 GAAGAC GAAGAC(2/6) 93 GTCTTC BspLU11I 314 ACATGT A^CATGT 1791 ACATGT BspMI 2088 ACCTGC ACCTGC(4/8) 298 GCAGGT BsrBI 57 CCGCTC CCGCTC(-3/-3) 1825 GAGCGG BsrFI 952 GCCGGT [GA]^CCGG[CT] 2192 ACCGGC BstXI 566 CCAAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1754 CCACACTGGTGG Cfr10I 952 GCCGGT [GA]^CCGG[CT] 2192 ACCGGC HincII 652 GTTGAC GT[CT]^[GA]AC 1696 GTTGAC HindII 652 GTTGAC GT[CT]^[GA]AC 1696 GTTGAC MslI 1605 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1755 CACACTGGTG PpuMI 1037 AGGTCCT [GA]G^G[AT]CC[CT] 1349 AGGACCT SfcI 1574 CTGTAG C^T[GA][CT]AG 2090 CTGCAG StuI 885 AGGCCT AGG^CCT 1010 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AvaII BanI BbvI BfaI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PvuII Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII