(Circular) (Six-base) MAPSORT of: pFN21AE4576.seq Check: 2698 from: 1 to: 6994 pFN21AE4576 6994 bp With 182 enzymes: SgfI * March 28, 2012 14:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2294 4102 2294 Size: 1808 5186 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4804 278 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4337 1937 Size: 2400 4594 Acc65I G'GTAC_C Cuts at: 2513 4315 2513 Size: 1802 5192 AclI AA'CG_TT Cuts at: 1563 5121 5494 1563 Size: 3558 373 3063 Fragments arranged by size: 3558 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2035 2541 3265 3558 3693 4001 4020 5057 Size: 403 506 724 293 135 308 19 1037 Cuts at: 5057 6441 6709 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1037 724 506 403 308 293 268 135 19 AflII C'TTAA_G Cuts at: 820 1017 3365 820 Size: 197 2348 4449 Fragments arranged by size: 4449 2348 197 AflIII A'CryG_T Cuts at: 1203 2319 3796 5893 6870 1203 Size: 1116 1477 2097 977 1327 Fragments arranged by size: 2097 1477 1327 1116 977 AgeI A'CCGG_T Cuts at: 5825 5825 Size: 6994 AhdI GACnn_n'nnGTC Cuts at: 5723 5723 Size: 6994 AleI CACnn'nnGTG Cuts at: 1166 3764 1166 Size: 2598 4396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4081 1184 Size: 2897 4097 AlwNI CAG_nnn'CTG Cuts at: 2309 3046 3529 4737 6309 2309 Size: 737 483 1208 1572 2994 Fragments arranged by size: 2994 1572 1208 737 483 ApaLI G'TGCA_C Cuts at: 5051 6207 5051 Size: 1156 5838 ApoI r'AATT_y Cuts at: 1493 2981 3062 3949 4303 4529 4681 6790 1493 Size: 1488 81 887 354 226 152 2109 1697 Fragments arranged by size: 2109 1697 1488 887 354 226 152 81 AseI AT'TA_AT Cuts at: 160 1032 5548 160 Size: 872 4516 1606 Fragments arranged by size: 4516 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 AvaI C'yCGr_G Cuts at: 1870 1943 2067 4319 1870 Size: 73 124 2252 4545 Fragments arranged by size: 4545 2252 124 73 AvrII C'CTAG_G Cuts at: 3886 3886 Size: 6994 BamHI G'GATC_C Cuts at: 2055 2432 4324 4691 2055 Size: 377 1892 367 4358 Fragments arranged by size: 4358 1892 377 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2513 3752 4178 4315 5770 Size: 325 217 627 726 1239 426 137 1455 Cuts at: 5770 618 Size: 1842 Fragments arranged by size: 1842 1455 1239 726 627 426 325 217 137 BanII G_rGCy'C Cuts at: 1391 3073 4313 1391 Size: 1682 1240 4072 Fragments arranged by size: 4072 1682 1240 BbeI G_GCGC'C Cuts at: 4182 4182 Size: 6994 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 4023 928 Size: 971 192 1932 3899 Fragments arranged by size: 3899 1932 971 192 BbvCI CC'TCA_GC Cuts at: 2033 2033 Size: 6994 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5185 5219 1903 Size: 34 3248 34 3678 Fragments arranged by size: 3678 3248 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4886 6096 4886 Size: 1210 5784 BclI T'GATC_A Cuts at: 1548 2251 1548 Size: 703 6291 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2018 5605 Size: 107 122 71 631 605 337 9 3587 Cuts at: 5605 136 Size: 1525 Fragments arranged by size: 3587 1525 631 605 337 122 107 71 9 BglII A'GATC_T Cuts at: 2769 3808 6989 2769 Size: 1039 3181 2774 Fragments arranged by size: 3181 2774 1039 BlpI GC'TnA_GC Cuts at: 4411 4411 Size: 6994 Bme1580I G_kGCm'C Cuts at: 1790 5055 6211 1790 Size: 3265 1156 2573 Fragments arranged by size: 3265 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6994 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4725 5683 Size: 416 53 83 399 24 1886 1422 958 Cuts at: 5683 442 Size: 1753 Fragments arranged by size: 1886 1753 1422 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4256 1056 Size: 3200 3794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2682 3551 3674 5654 1239 Size: 1443 869 123 1980 2579 Fragments arranged by size: 2579 1980 1443 869 123 Bpu10I CC'TnA_GC Cuts at: 2033 2033 Size: 6994 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2474 3578 4187 5119 5984 6282 2474 Size: 1104 609 932 865 298 3186 Fragments arranged by size: 3186 1104 932 865 609 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3596 5657 882 Size: 617 2097 2061 2219 Fragments arranged by size: 2219 2097 2061 617 BsaAI yAC'GTr Cuts at: 493 1204 4785 493 Size: 711 3581 2702 Fragments arranged by size: 3581 2702 711 BsaBI GATnn'nnATC Cuts at: 2027 6988 2027 Size: 4961 2033 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4179 4801 5183 5818 Size: 53 83 186 936 2646 622 382 635 Cuts at: 5818 275 Size: 1451 Fragments arranged by size: 2646 1451 936 635 622 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5427 5825 6099 6246 6657 5427 Size: 398 274 147 411 5764 Fragments arranged by size: 5764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2979 3009 1178 Size: 30 165 30 1576 30 5163 Fragments arranged by size: 5163 1576 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2460 1186 Size: 1274 5720 BseYI C'CCAG_C Cuts at: 2530 6197 6693 2530 Size: 3667 496 2831 Fragments arranged by size: 3667 2831 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2649 2886 3129 1163 Size: 573 913 237 243 5028 Fragments arranged by size: 5028 913 573 243 237 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3546 4444 5205 6233 1540 Size: 275 186 1545 898 761 1028 2301 Fragments arranged by size: 2301 1545 1028 898 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3073 5055 5140 6211 3073 Size: 1982 85 1071 3856 Fragments arranged by size: 3856 1982 1071 85 BsmI GAATG_Cn' Cuts at: 2622 4505 4598 2622 Size: 1883 93 5018 Fragments arranged by size: 5018 1883 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3073 4313 5055 5140 6211 1391 Size: 399 1283 1240 742 85 1071 2174 Fragments arranged by size: 2174 1283 1240 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6657 6657 Size: 6994 BspHI T'CATG_A Cuts at: 4883 6613 4883 Size: 1730 5264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2294 4102 4349 844 Size: 1450 1808 247 3489 Fragments arranged by size: 3489 1808 1450 247 BsrBI CCG'CTC Cuts at: 1394 1823 2064 3832 4881 1394 Size: 429 241 1768 1049 3507 Fragments arranged by size: 3507 1768 1049 429 241 BsrDI GCAATG_nn' Cuts at: 65 1248 5489 5663 65 Size: 1183 4241 174 1396 Fragments arranged by size: 4241 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2957 4197 5638 5825 1700 Size: 110 1147 1240 1441 187 2869 Fragments arranged by size: 2869 1441 1240 1147 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6994 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6994 BssSI C'ACGA_G Cuts at: 5054 6066 5054 Size: 1012 5982 BstAPI GCAn_nnn'nTGC Cuts at: 794 3376 794 Size: 2582 4412 BstBI TT'CG_AA Cuts at: 2161 5798 2161 Size: 3637 3357 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6994 BstXI CCAn_nnnn'nTGG Cuts at: 2578 3766 2578 Size: 1188 5806 BstYI r'GATC_y Cuts at: 1972 2055 2432 2769 3808 4080 4168 4324 4691 Size: 83 377 337 1039 272 88 156 367 Cuts at: 4691 5076 5093 6545 6631 6643 6989 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1039 385 377 367 346 337 272 156 88 86 83 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3034 3144 3510 513 Size: 552 939 1030 110 366 3997 Fragments arranged by size: 3997 1030 939 552 366 110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3115 487 Size: 683 56 1889 4366 Fragments arranged by size: 4366 1889 683 56 BtsI GCAGTG_nn' Cuts at: 3186 4510 5304 5324 3186 Size: 1324 794 20 4856 Fragments arranged by size: 4856 1324 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6994 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3718 3753 529 Size: 35 3154 35 3770 Fragments arranged by size: 3770 3154 35 35 DraI TTT'AAA Cuts at: 4298 4298 Size: 6994 DraIII CAC_nnn'GTG Cuts at: 3376 3376 Size: 6994 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6001 809 Size: 507 4685 1802 Fragments arranged by size: 4685 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4086 4441 5330 Size: 54 1425 50 275 18 2256 355 889 Cuts at: 5330 6959 8 Size: 1629 43 Fragments arranged by size: 2256 1629 1425 889 355 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4441 1537 Size: 275 2629 4090 Fragments arranged by size: 4090 2629 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3929 4924 1353 Size: 2576 995 3423 Fragments arranged by size: 3423 2576 995 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3818 4019 5581 5955 6101 387 Size: 1064 138 2229 201 1562 374 146 1280 Fragments arranged by size: 2229 1562 1280 1064 374 201 146 138 EcoICRI GAG'CTC Cuts at: 3071 3071 Size: 6994 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2541 2682 3265 3551 3558 3674 Size: 393 403 506 141 583 286 7 116 Cuts at: 3674 3693 4001 4020 5057 5654 6441 6709 1239 Size: 19 308 19 1037 597 787 268 1524 Fragments arranged by size: 1524 1037 787 597 583 506 403 393 308 286 268 141 116 19 19 7 EcoNI CCTnn'n_nnAGG Cuts at: 4073 4073 Size: 6994 EcoO109I rG'GnC_Cy Cuts at: 2535 3043 3355 4151 2535 Size: 508 312 796 5378 Fragments arranged by size: 5378 796 508 312 EcoRI G'AATT_C Cuts at: 3949 4303 4681 3949 Size: 354 378 6262 Fragments arranged by size: 6262 378 354 EcoRV GAT'ATC Cuts at: 2314 2314 Size: 6994 FspI TGC'GCA Cuts at: 5500 5500 Size: 6994 HaeII r_GCGC'y Cuts at: 1678 4182 6141 1678 Size: 2504 1959 2531 Fragments arranged by size: 2531 2504 1959 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3114 3146 3391 3423 5635 Size: 32 135 32 1542 32 245 32 2212 Cuts at: 5635 5667 5709 5741 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2212 1542 245 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2659 3703 4338 4584 669 Size: 988 281 721 1044 635 246 3079 Fragments arranged by size: 3079 1044 988 721 635 281 246 HindIII A'AGCT_T Cuts at: 748 3733 748 Size: 2985 4009 HpaI GTT'AAC Cuts at: 4584 4584 Size: 6994 KasI G'GCGC_C Cuts at: 4178 4178 Size: 6994 KpnI G_GTAC'C Cuts at: 2517 4319 2517 Size: 1802 5192 MfeI C'AATT_G Cuts at: 4593 4593 Size: 6994 MluI A'CGCG_T Cuts at: 6870 6870 Size: 6994 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6108 6292 1305 Size: 4803 184 2007 Fragments arranged by size: 4803 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4088 10 Size: 54 1425 343 2256 2916 Fragments arranged by size: 2916 2256 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3614 3764 4952 5311 5470 518 Size: 648 2448 150 1188 359 159 2042 Fragments arranged by size: 2448 2042 1188 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 3526 3578 4249 4410 5087 6235 6480 1606 Size: 1920 52 671 161 677 1148 245 2120 Fragments arranged by size: 2120 1920 1148 677 671 245 161 52 NarI GG'CG_CC Cuts at: 4179 4179 Size: 6994 NcoI C'CATG_G Cuts at: 513 1065 2004 3034 513 Size: 552 939 1030 4473 Fragments arranged by size: 4473 1030 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6994 NheI G'CTAG_C Cuts at: 1052 4252 1052 Size: 3200 3794 NotI GC'GGCC_GC Cuts at: 4441 4441 Size: 6994 NspI r_CATG'y Cuts at: 1132 1229 2323 3496 3800 4352 5897 1132 Size: 97 1094 1173 304 552 1545 2229 Fragments arranged by size: 2229 1545 1173 1094 552 304 97 PasI CC'CwG_GG Cuts at: 1348 2677 1348 Size: 1329 5665 PciI A'CATG_T Cuts at: 2319 3796 5893 2319 Size: 1477 2097 3420 Fragments arranged by size: 3420 2097 1477 PflMI CCAn_nnn'nTGG Cuts at: 1108 2668 1108 Size: 1560 5434 PfoI T'CCnGG_A Cuts at: 3690 3690 Size: 6994 PmeI GTTT'AAAC Cuts at: 4298 4298 Size: 6994 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5063 5095 6576 6608 5063 Size: 32 1481 32 5449 Fragments arranged by size: 5449 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3043 3355 3043 Size: 312 6682 PshAI GACnn'nnGTC Cuts at: 1613 3360 1613 Size: 1747 5247 PsiI TTA'TAA Cuts at: 4564 4564 Size: 6994 PstI C_TGCA'G Cuts at: 830 4099 4346 830 Size: 3269 247 3478 Fragments arranged by size: 3478 3269 247 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6994 PvuII CAG'CTG Cuts at: 3526 3578 4249 3526 Size: 52 671 6271 Fragments arranged by size: 6271 671 52 SacI G_AGCT'C Cuts at: 3073 3073 Size: 6994 SalI G'TCGA_C Cuts at: 1936 4336 1936 Size: 2400 4594 SbfI CC_TGCA'GG Cuts at: 4346 4346 Size: 6994 ScaI AGT'ACT Cuts at: 5242 6954 5242 Size: 1712 5282 SexAI A'CCwGG_T Cuts at: 3647 4290 3647 Size: 643 6351 SfcI C'TryA_G Cuts at: 826 1045 3579 4095 4342 5477 6158 6349 826 Size: 219 2534 516 247 1135 681 191 1471 Fragments arranged by size: 2534 1471 1135 681 516 247 219 191 SfoI GGC'GCC Cuts at: 4180 4180 Size: 6994 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6994 SmaI CCC'GGG Cuts at: 1872 4321 1872 Size: 2449 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 3365 3593 4202 5098 5999 Size: 197 926 510 912 228 609 896 901 Cuts at: 5999 6261 820 Size: 262 1553 Fragments arranged by size: 1553 926 912 901 896 609 510 262 228 197 SnaBI TAC'GTA Cuts at: 493 4785 493 Size: 4292 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6994 SphI G_CATG'C Cuts at: 1132 3496 4352 1132 Size: 2364 856 3774 Fragments arranged by size: 3774 2364 856 SspI AAT'ATT Cuts at: 5 52 4918 5 Size: 47 4866 2081 Fragments arranged by size: 4866 2081 47 StuI AGG'CCT Cuts at: 1517 2892 3017 1517 Size: 1375 125 5494 Fragments arranged by size: 5494 1375 125 StyI C'CwwG_G Cuts at: 513 1065 2004 3034 3310 3876 3886 4433 513 Size: 552 939 1030 276 566 10 547 3074 Fragments arranged by size: 3074 1030 939 566 552 547 276 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2570 3213 3560 3740 5021 5038 5191 Size: 1255 990 643 347 180 1281 17 153 Cuts at: 5191 5376 5821 325 Size: 185 445 1498 Fragments arranged by size: 1498 1281 1255 990 643 445 347 185 180 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5240 6952 96 Size: 274 80 33 51 1443 3263 1712 138 Fragments arranged by size: 3263 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6954 6986 6954 Size: 32 6962 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6994 XbaI T'CTAG_A Cuts at: 4330 4330 Size: 6994 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2129 2129 Size: 6994 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6994 XmaI C'CCGG_G Cuts at: 1870 4319 1870 Size: 2449 4545 XmnI GAAnn'nnTTC Cuts at: 5123 5123 Size: 6994 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4802 276 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI