################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AccIII AclI AcsI AflII AflIII Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AvrII BamHI BfaI BfrI BlnI BsaBI BseAI BsiWI Bsp1407I BspEI BspLU11I BsrGI BssHII BstBI CpoI DraI DraIII EcoRI EcoT22I HindIII HpaI MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SbfI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 599 GGTACC G^GTACC AgeI 1180 ACCGGT A^CCGGT AhdI 2338 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 599 GGTACC G^GTACC AspEI 2338 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 2698 CACGTG CAC^GTG BsaI 2564 GAGACC GGTCTC(1/5) BsmBI 673 CGTCTC CGTCTC(1/5) BsmI 3132 GAATGC GAATGC(1/-1) BspDI 2626 ATCGAT AT^CGAT BspHI 1829 TCATGA T^CATGA BsrDI 918 CATTGC GCAATG(2/0) Bst1107I 2098 GTATAC GTA^TAC BstZ17I 2098 GTATAC GTA^TAC ClaI 2626 ATCGAT AT^CGAT DrdI 199 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2338 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 742 GTCGAC GT[CT]^[GA]AC HindII 742 GTCGAC GT[CT]^[GA]AC KpnI 599 GGTACC GGTAC^C KspI 2333 CCGCGG CCGC^GG NcoI 907 CCATGG C^CATGG PinAI 1180 ACCGGT A^CCGGT PmaCI 2698 CACGTG CAC^GTG PmlI 2698 CACGTG CAC^GTG RcaI 1829 TCATGA T^CATGA SacI 799 GAGCTC GAGCT^C SacII 2333 CCGCGG CCGC^GG SalI 742 GTCGAC G^TCGAC SapI 2436 GCTCTTC GCTCTTC(1/4) ScaI 2864 AGTACT AGT^ACT SexAI 2525 ACCTGGT A^CC[AT]GGT SfiI 2013 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1237 CTCAAG C^T[CT][GA]AG TfiI 2285 GATTC G^A[AT]TC Tsp509I 98 AATT ^AATT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BbeI 2045 GGCGCC GGCGC^C 2113 GGCGCC BbsI 1941 GAAGAC GAAGAC(2/6) 3268 GAAGAC BbvCI 1626 CCTCAGC CCTCAGC(-5/-2) 2305 GCTGAGG BcgI 2925 CGAGGGCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2618 GCAACCGCATCG BciVI 1666 GTATCC GTATCC(6/5) 3121 GTATCC BclI 1175 TGATCA T^GATCA 2774 TGATCA BglII 1007 AGATCT A^GATCT 2144 AGATCT BlpI 936 GCTCAGC GC^T[ACGT]AGC 3054 GCTCAGC Bpu1102I 936 GCTCAGC GC^T[ACGT]AGC 3054 GCTCAGC BpuAI 1941 GAAGAC GAAGAC(2/6) 3268 GAAGAC BsaAI 2698 CACGTG [CT]AC^GT[GA] 2770 TACGTG BstEII 387 GGTCACC G^GT[ACGT]ACC 484 GGTGACC BstPI 387 GGTCACC G^GT[ACGT]ACC 484 GGTGACC CelII 936 GCTCAGC GC^T[ACGT]AGC 3054 GCTCAGC EagI 554 CGGCCG C^GGCCG 960 CGGCCG EclXI 554 CGGCCG C^GGCCG 960 CGGCCG Eco52I 554 CGGCCG C^GGCCG 960 CGGCCG EcoO65I 387 GGTCACC G^GT[ACGT]ACC 484 GGTGACC EcoRV 946 GATATC GAT^ATC 2629 GATATC FbaI 1175 TGATCA T^GATCA 2774 TGATCA FseI 551 GGCCGGCC GGCCGG^CC 1272 GGCCGGCC KasI 2045 GGCGCC G^GCGCC 2113 GGCGCC NarI 2045 GGCGCC GG^CGCC 2113 GGCGCC SfoI 2045 GGCGCC GGC^GCC 2113 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AatII AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI AspHI AspI AvaI AvaII AviII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI