(Linear) (Six-base) MAPSORT of: KIEE4591.seq Check: 2163 from: 1 to: 3321 KIEE4591.orf With 182 enzymes: SgfI * March 22, 2012 18:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1371 3321 Size: 1371 1950 AatII G_ACGT'C Cuts at: 0 1787 2175 2600 3206 3321 Size: 1787 388 425 606 115 Fragments arranged by size: 1787 606 425 388 115 AccI GT'mk_AC Cuts at: 0 743 2099 2390 3321 Size: 743 1356 291 931 Fragments arranged by size: 1356 931 743 291 Acc65I G'GTAC_C Cuts at: 0 599 3321 Size: 599 2722 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 1558 2098 3321 Size: 850 708 540 1223 Fragments arranged by size: 1223 850 708 540 AfeI AGC'GCT Cuts at: 0 257 2422 3148 3178 3321 Size: 257 2165 726 30 143 Fragments arranged by size: 2165 726 257 143 30 AgeI A'CCGG_T Cuts at: 0 1180 3321 Size: 1180 2141 AhdI GACnn_n'nnGTC Cuts at: 0 2343 3321 Size: 2343 978 AleI CACnn'nnGTG Cuts at: 0 482 1618 3321 Size: 482 1136 1703 Fragments arranged by size: 1703 1136 482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 935 2995 3268 3321 Size: 935 2060 273 53 Fragments arranged by size: 2060 935 273 53 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 674 706 2812 2844 3321 Size: 674 32 2106 32 477 Fragments arranged by size: 2106 674 477 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1386 1887 1977 2055 2883 3285 3321 Size: 1386 501 90 78 828 402 36 Fragments arranged by size: 1386 828 501 402 90 78 36 ApaI G_GGCC'C Cuts at: 0 32 54 319 344 473 1230 3321 Size: 32 22 265 25 129 757 2091 Fragments arranged by size: 2091 757 265 129 32 25 22 AvaI C'yCGr_G Cuts at: 0 31 472 581 1223 1870 2734 3092 3321 Size: 31 441 109 642 647 864 358 229 Fragments arranged by size: 864 647 642 441 358 229 109 31 BanI G'GyrC_C Cuts at: 0 36 599 1093 2045 2113 3321 Size: 36 563 494 952 68 1208 Fragments arranged by size: 1208 952 563 494 68 36 BanII G_rGCy'C Cuts at: 0 32 54 278 319 344 473 803 1046 Size: 32 22 224 41 25 129 330 243 Cuts at: 1046 1230 1766 2915 3321 Size: 184 536 1149 406 Fragments arranged by size: 1149 536 406 330 243 224 184 129 41 32 25 22 BbeI G_GCGC'C Cuts at: 0 2049 2117 3321 Size: 2049 68 1204 Fragments arranged by size: 2049 1204 68 BbsI GAAGACnn'nnnn_ Cuts at: 0 1948 3275 3321 Size: 1948 1327 46 Fragments arranged by size: 1948 1327 46 BbvCI CC'TCA_GC Cuts at: 0 1627 2306 3321 Size: 1627 679 1015 Fragments arranged by size: 1627 1015 679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2607 2641 2914 2948 3321 Size: 2607 34 273 34 373 Fragments arranged by size: 2607 373 273 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1677 3132 3321 Size: 1677 1455 189 Fragments arranged by size: 1677 1455 189 BclI T'GATC_A Cuts at: 0 1175 2774 3321 Size: 1175 1599 547 Fragments arranged by size: 1599 1175 547 BglI GCCn_nnn'nGGC Cuts at: 0 339 562 968 1560 2020 2288 2370 3321 Size: 339 223 406 592 460 268 82 951 Fragments arranged by size: 951 592 460 406 339 268 223 82 BglII A'GATC_T Cuts at: 0 1007 2144 3321 Size: 1007 1137 1177 Fragments arranged by size: 1177 1137 1007 BlpI GC'TnA_GC Cuts at: 0 937 3055 3321 Size: 937 2118 266 Fragments arranged by size: 2118 937 266 Bme1580I G_kGCm'C Cuts at: 0 32 41 54 319 344 473 611 712 Size: 32 9 13 265 25 129 138 101 Cuts at: 712 1083 1230 1625 1998 3321 Size: 371 147 395 373 1323 Fragments arranged by size: 1323 395 373 371 265 147 138 129 101 32 25 13 9 BmgBI CAC'GTC Cuts at: 0 867 882 1173 1464 3321 Size: 867 15 291 291 1857 Fragments arranged by size: 1857 867 291 291 15 BmrI ACTGGGnnnn_n' Cuts at: 0 557 1897 2349 3321 Size: 557 1340 452 972 Fragments arranged by size: 1340 972 557 452 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 420 3168 3321 Size: 420 2748 153 Fragments arranged by size: 2748 420 153 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 817 877 1588 1999 2161 2395 2506 Size: 205 612 60 711 411 162 234 111 Cuts at: 2506 2857 2893 3160 3321 Size: 351 36 267 161 Fragments arranged by size: 711 612 411 351 267 234 205 162 161 111 60 36 Bpu10I CC'TnA_GC Cuts at: 0 980 986 1627 2306 3321 Size: 980 6 641 679 1015 Fragments arranged by size: 1015 980 679 641 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1222 3321 Size: 1222 2099 BsaI GGTCTCn'nnnn_ Cuts at: 0 2558 3321 Size: 2558 763 BsaAI yAC'GTr Cuts at: 0 2700 2772 3321 Size: 2700 72 549 Fragments arranged by size: 2700 549 72 BsaHI Gr'CG_yC Cuts at: 0 1784 2046 2114 2172 2456 2597 3203 3321 Size: 1784 262 68 58 284 141 606 118 Fragments arranged by size: 1784 606 284 262 141 118 68 58 BsaWI w'CCGG_w Cuts at: 0 755 897 1180 3321 Size: 755 142 283 2141 Fragments arranged by size: 2141 755 283 142 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 160 190 601 631 1739 1769 2197 2227 Size: 160 30 411 30 1108 30 428 30 Cuts at: 2227 2254 2284 3321 Size: 27 30 1037 Fragments arranged by size: 1108 1037 428 411 160 30 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 118 139 175 178 181 1135 1800 2433 Size: 118 21 36 3 3 954 665 633 Cuts at: 2433 3157 3321 Size: 724 164 Fragments arranged by size: 954 724 665 633 164 118 36 21 3 3 BseYI C'CCAG_C Cuts at: 0 318 450 715 804 808 1017 1028 1670 Size: 318 132 265 89 4 209 11 642 Cuts at: 1670 2636 2746 3321 Size: 966 110 575 Fragments arranged by size: 966 642 575 318 265 209 132 110 89 11 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1123 1384 1486 1862 3178 3195 3321 Size: 1123 261 102 376 1316 17 126 Fragments arranged by size: 1316 1123 376 261 126 102 17 BsiEI CG_ry'CG Cuts at: 0 557 963 2466 2562 2760 3321 Size: 557 406 1503 96 198 561 Fragments arranged by size: 1503 561 557 406 198 96 BsiHKAI G_wGCw'C Cuts at: 0 374 479 580 803 938 2843 2984 3056 Size: 374 105 101 223 135 1905 141 72 Cuts at: 3056 3321 Size: 265 Fragments arranged by size: 1905 374 265 223 141 135 105 101 72 BsmI GAATG_Cn' Cuts at: 0 3138 3321 Size: 3138 183 BsmBI CGTCTCn'nnnn_ Cuts at: 0 679 3321 Size: 679 2642 Bsp1286I G_dGCh'C Cuts at: 0 32 41 54 278 319 344 374 473 Size: 32 9 13 224 41 25 30 99 Cuts at: 473 479 580 611 712 803 938 1046 1083 Size: 6 101 31 101 91 135 108 37 Cuts at: 1083 1230 1625 1766 1998 2843 2915 2984 3056 Size: 147 395 141 232 845 72 69 72 Cuts at: 3056 3321 Size: 265 Fragments arranged by size: 845 395 265 232 224 147 141 135 108 101 101 99 91 72 72 69 41 37 32 31 30 25 13 9 6 BspHI T'CATG_A Cuts at: 0 1829 3321 Size: 1829 1492 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 195 497 1136 1371 1496 1559 1643 1644 Size: 195 302 639 235 125 63 84 1 Cuts at: 1644 1994 2411 2618 3321 Size: 350 417 207 703 Fragments arranged by size: 703 639 417 350 302 235 207 195 125 84 63 1 BsrBI CCG'CTC Cuts at: 0 990 1409 1572 3321 Size: 990 419 163 1749 Fragments arranged by size: 1749 990 419 163 BsrDI GCAATG_nn' Cuts at: 0 917 3321 Size: 917 2404 BsrFI r'CCGG_y Cuts at: 0 552 958 1180 1273 1325 2683 3321 Size: 552 406 222 93 52 1358 638 Fragments arranged by size: 1358 638 552 406 222 93 52 BssSI C'ACGA_G Cuts at: 0 655 1693 2515 3196 3321 Size: 655 1038 822 681 125 Fragments arranged by size: 1038 822 681 655 125 BstAPI GCAn_nnn'nTGC Cuts at: 0 1386 1689 2474 2847 3032 3041 3285 3321 Size: 1386 303 785 373 185 9 244 36 Fragments arranged by size: 1386 785 373 303 244 185 36 9 BstEII G'GTnAC_C Cuts at: 0 387 484 3321 Size: 387 97 2837 Fragments arranged by size: 2837 387 97 BstXI CCAn_nnnn'nTGG Cuts at: 0 1833 2199 2373 2796 3321 Size: 1833 366 174 423 525 Fragments arranged by size: 1833 525 423 366 174 BstYI r'GATC_y Cuts at: 0 225 752 1007 1973 2144 3321 Size: 225 527 255 966 171 1177 Fragments arranged by size: 1177 966 527 255 225 171 BstZ17I GTA'TAC Cuts at: 0 2100 3321 Size: 2100 1221 Bsu36I CC'TnA_GG Cuts at: 0 823 2570 3311 3321 Size: 823 1747 741 10 Fragments arranged by size: 1747 823 741 10 BtgI C'CryG_G Cuts at: 0 54 354 423 603 638 907 1595 1712 Size: 54 300 69 180 35 269 688 117 Cuts at: 1712 2333 3321 Size: 621 988 Fragments arranged by size: 988 688 621 300 269 180 117 69 54 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1776 2326 3321 Size: 1776 550 995 Fragments arranged by size: 1776 995 550 BtsI GCAGTG_nn' Cuts at: 0 853 1555 2531 2539 3175 3321 Size: 853 702 976 8 636 146 Fragments arranged by size: 976 853 702 636 146 8 ClaI AT'CG_AT Cuts at: 0 2627 3321 Size: 2627 694 DrdI GACnn_nn'nnGTC Cuts at: 0 205 3321 Size: 205 3116 EaeI y'GGCC_r Cuts at: 0 16 88 509 554 960 1271 1647 2021 Size: 16 72 421 45 406 311 376 374 Cuts at: 2021 2519 2708 2798 2885 3321 Size: 498 189 90 87 436 Fragments arranged by size: 498 436 421 406 376 374 311 189 90 87 72 45 16 EagI C'GGCC_G Cuts at: 0 554 960 3321 Size: 554 406 2361 Fragments arranged by size: 2361 554 406 EarI CTCTTCn'nnn_ Cuts at: 0 1305 1996 2443 3321 Size: 1305 691 447 878 Fragments arranged by size: 1305 878 691 447 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 877 3321 Size: 877 2444 EcoICRI GAG'CTC Cuts at: 0 801 3321 Size: 801 2520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 817 850 877 1558 1588 1999 2098 Size: 205 612 33 27 681 30 411 99 Cuts at: 2098 2161 2395 2506 2857 2893 3160 3321 Size: 63 234 111 351 36 267 161 Fragments arranged by size: 681 612 411 351 267 234 205 161 111 99 63 36 33 30 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 682 1132 1897 2923 3196 3321 Size: 682 450 765 1026 273 125 Fragments arranged by size: 1026 765 682 450 273 125 EcoO109I rG'GnC_Cy Cuts at: 0 28 60 108 207 340 341 993 1125 Size: 28 32 48 99 133 1 652 132 Cuts at: 1125 1916 2052 2481 2730 2820 3261 3321 Size: 791 136 429 249 90 441 60 Fragments arranged by size: 791 652 441 429 249 136 133 132 99 90 60 48 32 28 1 EcoRV GAT'ATC Cuts at: 0 948 2631 3321 Size: 948 1683 690 Fragments arranged by size: 1683 948 690 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2409 3321 Size: 2409 912 FseI GG_CCGG'CC Cuts at: 0 556 1277 3321 Size: 556 721 2044 Fragments arranged by size: 2044 721 556 FspI TGC'GCA Cuts at: 0 631 2497 2902 3244 3321 Size: 631 1866 405 342 77 Fragments arranged by size: 1866 631 405 342 77 HaeII r_GCGC'y Cuts at: 0 259 457 646 1063 2049 2077 2090 2117 Size: 259 198 189 417 986 28 13 27 Cuts at: 2117 2424 3150 3180 3294 3321 Size: 307 726 30 114 27 Fragments arranged by size: 986 726 417 307 259 198 189 114 30 28 27 27 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 160 192 1739 1771 1795 1827 1887 1919 Size: 160 32 1547 32 24 32 60 32 Cuts at: 1919 2254 2286 2329 2361 2587 2619 3321 Size: 335 32 43 32 226 32 702 Fragments arranged by size: 1547 702 335 226 160 60 43 32 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 0 744 3321 Size: 744 2577 KasI G'GCGC_C Cuts at: 0 2045 2113 3321 Size: 2045 68 1208 Fragments arranged by size: 2045 1208 68 KpnI G_GTAC'C Cuts at: 0 603 3321 Size: 603 2718 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 275 312 1310 2699 2795 3321 Size: 275 37 998 1389 96 526 Fragments arranged by size: 1389 998 526 275 96 37 MscI TGG'CCA Cuts at: 0 511 1649 2023 2521 2887 3321 Size: 511 1138 374 498 366 434 Fragments arranged by size: 1138 511 498 434 374 366 MslI CAynn'nnrTG Cuts at: 0 482 637 694 1618 1831 3131 3321 Size: 482 155 57 924 213 1300 190 Fragments arranged by size: 1300 924 482 213 190 155 57 MspA1I CmG'CkG Cuts at: 0 808 855 1674 2070 2199 2335 2640 2826 Size: 808 47 819 396 129 136 305 186 Cuts at: 2826 2883 3321 Size: 57 438 Fragments arranged by size: 819 808 438 396 305 186 136 129 57 47 NaeI GCC'GGC Cuts at: 0 554 960 1275 1327 2685 3321 Size: 554 406 315 52 1358 636 Fragments arranged by size: 1358 636 554 406 315 52 NarI GG'CG_CC Cuts at: 0 2046 2114 3321 Size: 2046 68 1207 Fragments arranged by size: 2046 1207 68 NcoI C'CATG_G Cuts at: 0 907 3321 Size: 907 2414 NgoMIV G'CCGG_C Cuts at: 0 552 958 1273 1325 2683 3321 Size: 552 406 315 52 1358 638 Fragments arranged by size: 1358 638 552 406 315 52 NspI r_CATG'y Cuts at: 0 594 1377 3243 3321 Size: 594 783 1866 78 Fragments arranged by size: 1866 783 594 78 PasI CC'CwG_GG Cuts at: 0 25 2319 3321 Size: 25 2294 1002 Fragments arranged by size: 2294 1002 25 PflMI CCAn_nnn'nTGG Cuts at: 0 294 312 1443 1977 2055 3321 Size: 294 18 1131 534 78 1266 Fragments arranged by size: 1266 1131 534 294 78 18 PfoI T'CCnGG_A Cuts at: 0 182 677 1292 1976 2054 3321 Size: 182 495 615 684 78 1267 Fragments arranged by size: 1267 684 615 495 182 78 PmlI CAC'GTG Cuts at: 0 2700 3321 Size: 2700 621 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 742 774 1891 1923 3321 Size: 742 32 1117 32 1398 Fragments arranged by size: 1398 1117 742 32 32 PpuMI rG'GwC_Cy Cuts at: 0 108 207 993 1125 1916 2052 2730 2820 Size: 108 99 786 132 791 136 678 90 Cuts at: 2820 3261 3321 Size: 441 60 Fragments arranged by size: 791 786 678 441 136 132 108 99 90 60 PspOMI G'GGCC_C Cuts at: 0 28 50 315 340 469 1226 3321 Size: 28 22 265 25 129 757 2095 Fragments arranged by size: 2095 757 265 129 28 25 22 PstI C_TGCA'G Cuts at: 0 152 176 1136 1382 1493 3321 Size: 152 24 960 246 111 1828 Fragments arranged by size: 1828 960 246 152 111 24 PvuII CAG'CTG Cuts at: 0 808 855 1674 2070 2199 2640 2883 3321 Size: 808 47 819 396 129 441 243 438 Fragments arranged by size: 819 808 441 438 396 243 129 47 SacI G_AGCT'C Cuts at: 0 803 3321 Size: 803 2518 SacII CC_GC'GG Cuts at: 0 2336 3321 Size: 2336 985 SalI G'TCGA_C Cuts at: 0 742 3321 Size: 742 2579 SanDI GG'GwC_CC Cuts at: 0 993 2730 3321 Size: 993 1737 591 Fragments arranged by size: 1737 993 591 SapI GCTCTTCn'nnn_ Cuts at: 0 2443 3321 Size: 2443 878 ScaI AGT'ACT Cuts at: 0 2866 3321 Size: 2866 455 SexAI A'CCwGG_T Cuts at: 0 2525 3321 Size: 2525 796 SfcI C'TryA_G Cuts at: 0 148 172 1132 1378 1489 2232 3321 Size: 148 24 960 246 111 743 1089 Fragments arranged by size: 1089 960 743 246 148 111 24 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2020 3321 Size: 2020 1301 SfoI GGC'GCC Cuts at: 0 2047 2115 3321 Size: 2047 68 1206 Fragments arranged by size: 2047 1206 68 SmaI CCC'GGG Cuts at: 0 33 1872 2736 3094 3321 Size: 33 1839 864 358 227 Fragments arranged by size: 1839 864 358 227 33 SmlI C'TyrA_G Cuts at: 0 1237 3321 Size: 1237 2084 StuI AGG'CCT Cuts at: 0 704 1138 3316 3321 Size: 704 434 2178 5 Fragments arranged by size: 2178 704 434 5 StyI C'CwwG_G Cuts at: 0 907 1097 1337 2009 2024 2246 2651 2789 Size: 907 190 240 672 15 222 405 138 Cuts at: 2789 3321 Size: 532 Fragments arranged by size: 907 672 532 405 240 222 190 138 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 472 908 1081 2331 2576 3321 Size: 472 436 173 1250 245 745 Fragments arranged by size: 1250 745 472 436 245 173 TatI w'GTAC_w Cuts at: 0 1397 1529 2213 2864 3321 Size: 1397 132 684 651 457 Fragments arranged by size: 1397 684 651 457 132 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 601 633 856 888 2599 2631 3321 Size: 601 32 223 32 1711 32 690 Fragments arranged by size: 1711 690 601 223 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 778 883 997 2272 2761 3321 Size: 778 105 114 1275 489 560 Fragments arranged by size: 1275 778 560 489 114 105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1797 2016 2056 2199 2247 3321 Size: 1797 219 40 143 48 1074 Fragments arranged by size: 1797 1074 219 143 48 40 XmaI C'CCGG_G Cuts at: 0 31 1870 2734 3092 3321 Size: 31 1839 864 358 229 Fragments arranged by size: 1839 864 358 229 31 ZraI GAC'GTC Cuts at: 0 1785 2173 2598 3204 3321 Size: 1785 388 425 606 117 Fragments arranged by size: 1785 606 425 388 117 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AgeI AhdI AleI AlfI AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SalI SanDI SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AclI AflII AflIII ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BfrBI BmtI BsaBI BsiWI BspEI BsrGI BssHII BstBI CspCI DraI DraIII EcoRI FspAI HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SbfI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI