################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AviII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BpmI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 1247 GGTACC G^GTACC AccII 1269 CGCG CG^CG AcsI 1285 GAATTT [GA]^AATT[CT] AflIII 995 ACGTGT A^C[GA][CT]GT ApaI 1325 GGGCCC GGGCC^C ApoI 1285 GAATTT [GA]^AATT[CT] Asp718I 1247 GGTACC G^GTACC AvrII 330 CCTAGG C^CTAGG BanI 1247 GGTACC G^G[CT][GA]CC BfaI 331 CTAG C^TAG BglI 1151 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 330 CCTAGG C^CTAGG BlpI 432 GCTCAGC GC^T[ACGT]AGC Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC BsaWI 595 TCCGGT [AT]^CCGG[AT] BsmBI 468 CGTCTC CGTCTC(1/5) BsmI 397 GCATTC GAATGC(1/-1) Bsp1407I 812 TGTACA T^GTACA BsrGI 812 TGTACA T^GTACA BssSI 883 CTCGTG CACGAG(-5/-1) BstAPI 1003 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1269 CGCG CG^CG BstXI 734 CCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 432 GCTCAGC GC^T[ACGT]AGC DraI 271 TTTAAA TTT^AAA DraII 1324 GGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 1103 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 519 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1324 GGGGCCC [GA]G^G[ACGT]CC[CT] KpnI 1247 GGTACC GGTAC^C MaeI 331 CTAG C^TAG MvnI 1269 CGCG CG^CG NlaIII 1278 CATG CATG^ PflMI 750 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 880 GAGCTC GAGCT^C SapI 183 GCTCTTC GCTCTTC(1/4) SexAI 496 ACCTGGT A^CC[AT]GGT SmaI 1328 CCCGGG CCC^GGG SspBI 812 TGTACA T^GTACA Tsp509I 1286 AATT ^AATT Van91I 750 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 734 CCACGGCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1328 CCCGGG C^CCGGG XmaI 1328 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Asp700I 684 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 855 GAAGACCTTC BbsI 855 GAAGAC GAAGAC(2/6) 970 GAAGAC BglII 752 AGATCT A^GATCT 1224 AGATCT BpuAI 855 GAAGAC GAAGAC(2/6) 970 GAAGAC BsaI 493 GAGACC GGTCTC(1/5) 1023 GGTCTC BsrBI 703 GAGCGG CCGCTC(-3/-3) 1060 GAGCGG EaeI 1178 CGGCCA [CT]^GGCC[GA] 1271 CGGCCA EcoT14I 330 CCTAGG C^C[AT][AT]GG 1030 CCAAGG FauI 705 GCGGG CCCGC(4/6) 1012 GCGGG HgaI 381 GACGC GACGC(5/10) 1122 GACGC MaeII 844 ACGT A^CGT 995 ACGT MseI 272 TTAA T^TAA 1217 TTAA MslI 735 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1126 CACAAATATG SfaNI 230 GCATC GCATC(5/9) 1310 GCATC SmlI 1171 CTCAAG C^T[CT][GA]AG 1228 CTCAAG StyI 330 CCTAGG C^C[AT][AT]GG 1030 CCAAGG Tru9I 272 TTAA T^TAA 1217 TTAA XmnI 684 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 855 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DrdI DsaI EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII