Restriction map for
pFN21AE4429
(Circular) (Six-base) MAPSORT of: pFN21AE4429.seq Check: 7872 from: 1 to: 6040
pFN21AE4429 6040 bp
With 182 enzymes: SgfI *
March 28, 2012 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3312 3312
Size: 6040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3252 3361 3252
Size: 109 5931
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2559 2879 4103 5487 5755 1632
Size: 927 320 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 927 320 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 3000 4939 5916 1203
Size: 1797 1939 977 1327
Fragments arranged by size:
1939 1797 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 2489
Size: 32 6008
AlwNI CAG_nnn'CTG
Cuts at: 2654 2948 2962 3014 3783 5355 2654
Size: 294 14 52 769 1572 3339
Fragments arranged by size:
3339 1572 769 294 52 14
ApaI G_GGCC'C
Cuts at: 3334 3334
Size: 6040
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 3290 3349 3575 3727 5836 1493
Size: 1797 59 226 152 2109 1697
Fragments arranged by size:
2109 1797 1697 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2915 3333 3365 1870
Size: 73 88 884 418 32 4545
Fragments arranged by size:
4545 884 418 88 73 32
AvrII C'CTAG_G
Cuts at: 2335 2335
Size: 6040
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3252 3361 4816 618
Size: 325 217 627 1465 109 1455 1842
Fragments arranged by size:
1842 1465 1455 627 325 217 109
BanII G_rGCy'C
Cuts at: 1391 2075 2889 3334 3359 1391
Size: 684 814 445 25 4072
Fragments arranged by size:
4072 814 684 445 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2867 2982 928
Size: 971 968 115 3986
Fragments arranged by size:
3986 971 968 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3162 4651 136
Size: 107 122 71 631 605 1490 1489 1525
Fragments arranged by size:
1525 1490 1489 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2757 3229 6035 2757
Size: 472 2806 2762
Fragments arranged by size:
2806 2762 472
BlpI GC'TnA_GC
Cuts at: 2438 3457 2438
Size: 1019 5021
Bme1580I G_kGCm'C
Cuts at: 1790 3334 4101 5257 1790
Size: 1544 767 1156 2573
Fragments arranged by size:
2573 1544 1156 767
BmgBI CAC'GTC
Cuts at: 1318 3001 1318
Size: 1683 4357
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3771 4729 442
Size: 416 53 83 399 24 2354 958 1753
Fragments arranged by size:
2354 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4700 1239
Size: 3461 2579
Bpu10I CC'TnA_GC
Cuts at: 2703 2703
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3161 3218 4165 5030 5328 3161
Size: 57 947 865 298 3873
Fragments arranged by size:
3873 947 865 298 57
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 3034 4703 882
Size: 617 993 542 1669 2219
Fragments arranged by size:
2219 1669 993 617 542
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2600 4473 4871 5145 5292 5703 2600
Size: 1873 398 274 147 411 2937
Fragments arranged by size:
2937 1873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2489 2519 2537 2567 2699
Size: 30 165 30 1086 30 18 30 132
Cuts at: 2699 2729 2876 2906 1178
Size: 30 147 30 4312
Fragments arranged by size:
4312 1086 165 147 132 30 30 30
30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2065 2092 2558 1186
Size: 879 27 466 4668
Fragments arranged by size:
4668 879 466 27
BseYI C'CCAG_C
Cuts at: 2681 3325 5243 5739 2681
Size: 644 1918 496 2982
Fragments arranged by size:
2982 1918 644 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2608 2889 3177 4101 4186 5257 2608
Size: 281 288 924 85 1071 3391
Fragments arranged by size:
3391 1071 924 288 281 85
BsmI GAATG_Cn'
Cuts at: 2402 3551 3644 2402
Size: 1149 93 4798
Fragments arranged by size:
4798 1149 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2479 2479
Size: 6040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2608 2889 3177 3334 3359 4101
Size: 399 285 533 281 288 157 25 742
Cuts at: 4101 4186 5257 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 533 399 288 285 281
157 85 25
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 2469 3312 3395 844
Size: 1349 276 843 83 3489
Fragments arranged by size:
3489 1349 843 276 83
BsrBI CCG'CTC
Cuts at: 1394 1823 2710 3067 3927 1394
Size: 429 887 357 860 3507
Fragments arranged by size:
3507 887 860 429 357
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2817 96
Size: 2721 3319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 2888 4100 5112 2888
Size: 1212 1012 3816
Fragments arranged by size:
3816 1212 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3014 794
Size: 2220 3820
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstXI CCAn_nnnn'nTGG
Cuts at: 2746 2746
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2757 2958 3229 3370 3737 4122 4139 5591
Size: 785 201 271 141 367 385 17 1452
Cuts at: 5591 5677 5689 6035 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 785 385 367 346 271 201
141 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2058 2581 2739 513
Size: 552 993 523 158 3814
Fragments arranged by size:
3814 993 552 523 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2563 487
Size: 683 56 1337 3964
Fragments arranged by size:
3964 1337 683 56
BtsI GCAGTG_nn'
Cuts at: 2751 3556 4350 4370 2751
Size: 805 794 20 4421
Fragments arranged by size:
4421 805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3268 3303 529
Size: 35 2704 35 3266
Fragments arranged by size:
3266 2704 35 35
DraI TTT'AAA
Cuts at: 2278 3344 2278
Size: 1066 4974
DraIII CAC_nnn'GTG
Cuts at: 3113 3113
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2300 2392 2519 5047 809
Size: 507 984 92 127 2528 1802
Fragments arranged by size:
2528 1802 984 507 127 92
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3183 3276 3487
Size: 54 1425 50 275 18 1353 93 211
Cuts at: 3487 4376 6005 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1353 889 275 211 93 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 2195 2535 2766 3970 1353
Size: 821 21 340 231 1204 3423
Fragments arranged by size:
3423 1204 821 340 231 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2106 2141 2711 4627 5001 5147
Size: 1064 138 517 35 570 1916 374 146
Cuts at: 5147 387
Size: 1280
Fragments arranged by size:
1916 1280 1064 570 517 374 146 138
35
EcoICRI GAG'CTC
Cuts at: 2887 2887
Size: 6040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2559 2879 4103 4700 5487 5755 1239
Size: 393 927 320 1224 597 787 268 1524
Fragments arranged by size:
1524 1224 927 787 597 393 320 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 6040
EcoO109I rG'GnC_Cy
Cuts at: 3330 3330
Size: 6040
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 3300 3332 4681
Size: 32 135 32 1076 32 620 32 1349
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1349 1076 620 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3256 3365 3256
Size: 109 5931
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 5154 5338 1305
Size: 1170 2679 184 2007
Fragments arranged by size:
2679 2007 1170 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4218
Fragments arranged by size:
4218 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3135 3998 4357 4516 518
Size: 648 1578 391 863 359 159 2042
Fragments arranged by size:
2042 1578 863 648 391 359 159
MspA1I CmG'CkG
Cuts at: 1606 3456 4133 5281 5526 1606
Size: 1850 677 1148 245 2120
Fragments arranged by size:
2120 1850 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5488
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3398 4943 1132
Size: 97 2169 1545 2229
Fragments arranged by size:
2229 2169 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2019 2068 3158 1348
Size: 671 49 1090 4230
Fragments arranged by size:
4230 1090 671 49
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2761 1108
Size: 1653 4387
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2489 2521 2606 2638 4109 4141 5622 5654 2489
Size: 32 85 32 1471 32 1481 32 2875
Fragments arranged by size:
2875 1481 1471 85 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PspOMI G'GGCC_C
Cuts at: 3330 3330
Size: 6040
PstI C_TGCA'G
Cuts at: 830 2355 2913 2967 3010 3392 830
Size: 1525 558 54 43 382 3478
Fragments arranged by size:
3478 1525 558 382 54 43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
SacI G_AGCT'C
Cuts at: 2889 2889
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SapI GCTCTTCn'nnn_
Cuts at: 2195 2195
Size: 6040
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SexAI A'CCwGG_T
Cuts at: 2501 2501
Size: 6040
SfcI C'TryA_G
Cuts at: 826 1045 2351 2909 2963 3006 3388 4523 5204
Size: 219 1306 558 54 43 382 1135 681
Cuts at: 5204 5395 826
Size: 191 1471
Fragments arranged by size:
1471 1306 1135 681 558 382 219 191
54 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3335 3367 1872
Size: 1463 32 4545
Fragments arranged by size:
4545 1463 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3176 3233 4144 5045 5307 820
Size: 197 926 1233 57 911 901 262 1553
Fragments arranged by size:
1553 1233 926 911 901 262 197 57
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SrfI GCCC'GGGC
Cuts at: 3335 3335
Size: 6040
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2335 3035 3479 513
Size: 552 1270 700 444 3074
Fragments arranged by size:
3074 1270 700 552 444
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2359 3093 4067 4084 4237 4422 4867
Size: 1255 779 734 974 17 153 185 445
Cuts at: 4867 325
Size: 1498
Fragments arranged by size:
1498 1255 974 779 734 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 2715 2817
Size: 274 80 33 51 1443 291 447 102
Cuts at: 2817 4286 5998 96
Size: 1469 1712 138
Fragments arranged by size:
1712 1469 1443 447 291 274 138 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2746 2746
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3333 3365 1870
Size: 1463 32 4545
Fragments arranged by size:
4545 1463 32
XmnI GAAnn'nnTTC
Cuts at: 2693 2864 4169 2693
Size: 171 1305 4564
Fragments arranged by size:
4564 1305 171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI
SwaI