(Circular) (Six-base) MAPSORT of: pFN21AE4429.seq Check: 7872 from: 1 to: 6040 pFN21AE4429 6040 bp With 182 enzymes: SgfI * March 28, 2012 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3312 3312 Size: 6040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 3252 3361 3252 Size: 109 5931 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2559 2879 4103 5487 5755 1632 Size: 927 320 1224 1384 268 1917 Fragments arranged by size: 1917 1384 1224 927 320 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 3000 4939 5916 1203 Size: 1797 1939 977 1327 Fragments arranged by size: 1939 1797 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 2744 1166 Size: 1578 4462 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 2489 Size: 32 6008 AlwNI CAG_nnn'CTG Cuts at: 2654 2948 2962 3014 3783 5355 2654 Size: 294 14 52 769 1572 3339 Fragments arranged by size: 3339 1572 769 294 52 14 ApaI G_GGCC'C Cuts at: 3334 3334 Size: 6040 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 3290 3349 3575 3727 5836 1493 Size: 1797 59 226 152 2109 1697 Fragments arranged by size: 2109 1797 1697 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2031 2915 3333 3365 1870 Size: 73 88 884 418 32 4545 Fragments arranged by size: 4545 884 418 88 73 32 AvrII C'CTAG_G Cuts at: 2335 2335 Size: 6040 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3252 3361 4816 618 Size: 325 217 627 1465 109 1455 1842 Fragments arranged by size: 1842 1465 1455 627 325 217 109 BanII G_rGCy'C Cuts at: 1391 2075 2889 3334 3359 1391 Size: 684 814 445 25 4072 Fragments arranged by size: 4072 814 684 445 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2867 2982 928 Size: 971 968 115 3986 Fragments arranged by size: 3986 971 968 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3162 4651 136 Size: 107 122 71 631 605 1490 1489 1525 Fragments arranged by size: 1525 1490 1489 631 605 122 107 71 BglII A'GATC_T Cuts at: 2757 3229 6035 2757 Size: 472 2806 2762 Fragments arranged by size: 2806 2762 472 BlpI GC'TnA_GC Cuts at: 2438 3457 2438 Size: 1019 5021 Bme1580I G_kGCm'C Cuts at: 1790 3334 4101 5257 1790 Size: 1544 767 1156 2573 Fragments arranged by size: 2573 1544 1156 767 BmgBI CAC'GTC Cuts at: 1318 3001 1318 Size: 1683 4357 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3771 4729 442 Size: 416 53 83 399 24 2354 958 1753 Fragments arranged by size: 2354 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4700 1239 Size: 3461 2579 Bpu10I CC'TnA_GC Cuts at: 2703 2703 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3161 3218 4165 5030 5328 3161 Size: 57 947 865 298 3873 Fragments arranged by size: 3873 947 865 298 57 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2492 3034 4703 882 Size: 617 993 542 1669 2219 Fragments arranged by size: 2219 1669 993 617 542 BsaAI yAC'GTr Cuts at: 493 1204 3831 493 Size: 711 2627 2702 Fragments arranged by size: 2702 2627 711 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3847 4229 4864 275 Size: 53 83 186 936 2314 382 635 1451 Fragments arranged by size: 2314 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2600 4473 4871 5145 5292 5703 2600 Size: 1873 398 274 147 411 2937 Fragments arranged by size: 2937 1873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2489 2519 2537 2567 2699 Size: 30 165 30 1086 30 18 30 132 Cuts at: 2699 2729 2876 2906 1178 Size: 30 147 30 4312 Fragments arranged by size: 4312 1086 165 147 132 30 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2065 2092 2558 1186 Size: 879 27 466 4668 Fragments arranged by size: 4668 879 466 27 BseYI C'CCAG_C Cuts at: 2681 3325 5243 5739 2681 Size: 644 1918 496 2982 Fragments arranged by size: 2982 1918 644 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2608 2889 3177 4101 4186 5257 2608 Size: 281 288 924 85 1071 3391 Fragments arranged by size: 3391 1071 924 288 281 85 BsmI GAATG_Cn' Cuts at: 2402 3551 3644 2402 Size: 1149 93 4798 Fragments arranged by size: 4798 1149 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2479 2479 Size: 6040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2608 2889 3177 3334 3359 4101 Size: 399 285 533 281 288 157 25 742 Cuts at: 4101 4186 5257 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 533 399 288 285 281 157 85 25 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2193 2469 3312 3395 844 Size: 1349 276 843 83 3489 Fragments arranged by size: 3489 1349 843 276 83 BsrBI CCG'CTC Cuts at: 1394 1823 2710 3067 3927 1394 Size: 429 887 357 860 3507 Fragments arranged by size: 3507 887 860 429 357 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2817 96 Size: 2721 3319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 2888 4100 5112 2888 Size: 1212 1012 3816 Fragments arranged by size: 3816 1212 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3014 794 Size: 2220 3820 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstXI CCAn_nnnn'nTGG Cuts at: 2746 2746 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2757 2958 3229 3370 3737 4122 4139 5591 Size: 785 201 271 141 367 385 17 1452 Cuts at: 5591 5677 5689 6035 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 785 385 367 346 271 201 141 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2058 2581 2739 513 Size: 552 993 523 158 3814 Fragments arranged by size: 3814 993 552 523 158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2563 487 Size: 683 56 1337 3964 Fragments arranged by size: 3964 1337 683 56 BtsI GCAGTG_nn' Cuts at: 2751 3556 4350 4370 2751 Size: 805 794 20 4421 Fragments arranged by size: 4421 805 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3268 3303 529 Size: 35 2704 35 3266 Fragments arranged by size: 3266 2704 35 35 DraI TTT'AAA Cuts at: 2278 3344 2278 Size: 1066 4974 DraIII CAC_nnn'GTG Cuts at: 3113 3113 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2300 2392 2519 5047 809 Size: 507 984 92 127 2528 1802 Fragments arranged by size: 2528 1802 984 507 127 92 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3183 3276 3487 Size: 54 1425 50 275 18 1353 93 211 Cuts at: 3487 4376 6005 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1353 889 275 211 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 2195 2535 2766 3970 1353 Size: 821 21 340 231 1204 3423 Fragments arranged by size: 3423 1204 821 340 231 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2106 2141 2711 4627 5001 5147 Size: 1064 138 517 35 570 1916 374 146 Cuts at: 5147 387 Size: 1280 Fragments arranged by size: 1916 1280 1064 570 517 374 146 138 35 EcoICRI GAG'CTC Cuts at: 2887 2887 Size: 6040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2559 2879 4103 4700 5487 5755 1239 Size: 393 927 320 1224 597 787 268 1524 Fragments arranged by size: 1524 1224 927 787 597 393 320 268 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 6040 EcoO109I rG'GnC_Cy Cuts at: 3330 3330 Size: 6040 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 5187 1678 Size: 3509 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 3300 3332 4681 Size: 32 135 32 1076 32 620 32 1349 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1349 1076 620 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3256 3365 3256 Size: 109 5931 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 5154 5338 1305 Size: 1170 2679 184 2007 Fragments arranged by size: 2679 2007 1170 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4218 Fragments arranged by size: 4218 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3135 3998 4357 4516 518 Size: 648 1578 391 863 359 159 2042 Fragments arranged by size: 2042 1578 863 648 391 359 159 MspA1I CmG'CkG Cuts at: 1606 3456 4133 5281 5526 1606 Size: 1850 677 1148 245 2120 Fragments arranged by size: 2120 1850 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5488 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3398 4943 1132 Size: 97 2169 1545 2229 Fragments arranged by size: 2229 2169 1545 97 PasI CC'CwG_GG Cuts at: 1348 2019 2068 3158 1348 Size: 671 49 1090 4230 Fragments arranged by size: 4230 1090 671 49 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2761 1108 Size: 1653 4387 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2489 2521 2606 2638 4109 4141 5622 5654 2489 Size: 32 85 32 1471 32 1481 32 2875 Fragments arranged by size: 2875 1481 1471 85 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PspOMI G'GGCC_C Cuts at: 3330 3330 Size: 6040 PstI C_TGCA'G Cuts at: 830 2355 2913 2967 3010 3392 830 Size: 1525 558 54 43 382 3478 Fragments arranged by size: 3478 1525 558 382 54 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 SacI G_AGCT'C Cuts at: 2889 2889 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SapI GCTCTTCn'nnn_ Cuts at: 2195 2195 Size: 6040 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SexAI A'CCwGG_T Cuts at: 2501 2501 Size: 6040 SfcI C'TryA_G Cuts at: 826 1045 2351 2909 2963 3006 3388 4523 5204 Size: 219 1306 558 54 43 382 1135 681 Cuts at: 5204 5395 826 Size: 191 1471 Fragments arranged by size: 1471 1306 1135 681 558 382 219 191 54 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3335 3367 1872 Size: 1463 32 4545 Fragments arranged by size: 4545 1463 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 3176 3233 4144 5045 5307 820 Size: 197 926 1233 57 911 901 262 1553 Fragments arranged by size: 1553 1233 926 911 901 262 197 57 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SrfI GCCC'GGGC Cuts at: 3335 3335 Size: 6040 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2335 3035 3479 513 Size: 552 1270 700 444 3074 Fragments arranged by size: 3074 1270 700 552 444 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2359 3093 4067 4084 4237 4422 4867 Size: 1255 779 734 974 17 153 185 445 Cuts at: 4867 325 Size: 1498 Fragments arranged by size: 1498 1255 974 779 734 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2268 2715 2817 Size: 274 80 33 51 1443 291 447 102 Cuts at: 2817 4286 5998 96 Size: 1469 1712 138 Fragments arranged by size: 1712 1469 1443 447 291 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2746 2746 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3333 3365 1870 Size: 1463 32 4545 Fragments arranged by size: 4545 1463 32 XmnI GAAnn'nnTTC Cuts at: 2693 2864 4169 2693 Size: 171 1305 4564 Fragments arranged by size: 4564 1305 171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SwaI