Restriction map for
pFN21AE1125
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AvrII BamHI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI EarI Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 360 TCCGGA T^CCGGA
AcyI 1287 GGCGCC G[GA]^CG[CT]C
AhdI 445 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 619 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 445 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1245 TGCGCA TGC^GCA
BalI 530 TGGCCA TGG^CCA
BbeI 1287 GGCGCC GGCGC^C
BbrPI 1675 CACGTG CAC^GTG
BbsI 205 GAAGAC GAAGAC(2/6)
BbvCI 1365 GCTGAGG CCTCAGC(-5/-2)
BglII 335 AGATCT A^GATCT
BpuAI 205 GAAGAC GAAGAC(2/6)
BsaAI 1675 CACGTG [CT]AC^GT[GA]
BsaHI 1287 GGCGCC G[GA]^CG[CT]C
BsaI 39 GGTCTC GGTCTC(1/5)
BsaWI 360 TCCGGA [AT]^CCGG[AT]
BseAI 360 TCCGGA T^CCGGA
BsgI 973 GTGCAG GTGCAG(16/14)
BsmBI 17 CGTCTC CGTCTC(1/5)
BspEI 360 TCCGGA T^CCGGA
BssHII 159 GCGCGC G^CGCGC
DraIII 988 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1373 CCACGG C^C[GA][CT]GG
EagI 1152 CGGCCG C^GGCCG
Eam1105I 445 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1152 CGGCCG C^GGCCG
Eco52I 1152 CGGCCG C^GGCCG
EcoT14I 595 CCTTGG C^C[AT][AT]GG
FspI 1245 TGCGCA TGC^GCA
HaeII 1287 GGCGCC [GA]GCGC^[CT]
Hin1I 1287 GGCGCC G[GA]^CG[CT]C
HincII 1138 GTCAAC GT[CT]^[GA]AC
HindII 1138 GTCAAC GT[CT]^[GA]AC
KasI 1287 GGCGCC G^GCGCC
MaeII 1676 ACGT A^CGT
MluNI 530 TGGCCA TGG^CCA
MroI 360 TCCGGA T^CCGGA
MscI 530 TGGCCA TGG^CCA
NarI 1287 GGCGCC GG^CGCC
NspI 1361 GCATGC [GA]CATG^[CT]
PmaCI 1675 CACGTG CAC^GTG
PmlI 1675 CACGTG CAC^GTG
PstI 566 CTGCAG CTGCA^G
PvuI 296 CGATCG CGAT^CG
SexAI 1019 ACCAGGT A^CC[AT]GGT
SfoI 1287 GGCGCC GGC^GCC
SphI 1361 GCATGC GCATG^C
StuI 1382 AGGCCT AGG^CCT
StyI 595 CCTTGG C^C[AT][AT]GG
Tsp509I 884 AATT ^AATT
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 484 GATCC GGATC(4/5)
859 GGATC
AocI 379 CCTGAGG CC^T[ACGT]AGG
694 CCTCAGG
AvaII 699 GGTCC G^G[AT]CC
1452 GGACC
BfaI 1201 CTAG C^TAG
1703 CTAG
BglI 173 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
753 GCCCCGAGGGC
BsiEI 296 CGATCG CG[GA][CT]^CG
1152 CGGCCG
BspMI 1231 ACCTGC ACCTGC(4/8)
1336 ACCTGC
BsrFI 1002 ACCGGC [GA]^CCGG[CT]
1463 ACCGGC
BstYI 335 AGATCT [GA]^GATC[CT]
859 GGATCT
Bsu36I 379 CCTGAGG CC^T[ACGT]AGG
694 CCTCAGG
Cfr10I 1002 ACCGGC [GA]^CCGG[CT]
1463 ACCGGC
Eco81I 379 CCTGAGG CC^T[ACGT]AGG
694 CCTCAGG
MaeI 1201 CTAG C^TAG
1703 CTAG
MflI 335 AGATCT [GA]^GATC[CT]
859 GGATCT
MslI 1237 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1404 CACCTGTGTG
MspA1I 1081 CAGCTG C[AC]G^C[GT]G
1207 CAGCTG
PvuII 1081 CAGCTG CAG^CTG
1207 CAGCTG
SfcI 566 CTGCAG C^T[GA][CT]AG
1272 CTACAG
XhoII 335 AGATCT [GA]^GATC[CT]
859 GGATCT
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
Asp700I AspHI AvaI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmaI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XmaCI
XmaI XmnI