Restriction map for
pFN21AE1125
(Linear) (Six-base) MAPSORT of: KIEE1125.seq Check: 9217 from: 1 to: 1716
KIEE1125.orf
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1240 1345 1716
Size: 1240 105 371
Fragments arranged by size:
1240 371 105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1716
Size: 1078 638
AhdI GACnn_n'nnGTC
Cuts at: 0 450 1716
Size: 450 1266
AleI CACnn'nnGTG
Cuts at: 0 1241 1408 1716
Size: 1241 167 308
Fragments arranged by size:
1241 308 167
AlwNI CAG_nnn'CTG
Cuts at: 0 624 1716
Size: 624 1092
ApaI G_GGCC'C
Cuts at: 0 156 764 1041 1049 1716
Size: 156 608 277 8 667
Fragments arranged by size:
667 608 277 156 8
AvaI C'yCGr_G
Cuts at: 0 121 148 497 755 968 1175 1503 1716
Size: 121 27 349 258 213 207 328 213
Fragments arranged by size:
349 328 258 213 213 207 121 27
BanI G'GyrC_C
Cuts at: 0 144 271 890 1287 1435 1716
Size: 144 127 619 397 148 281
Fragments arranged by size:
619 397 281 148 144 127
BanII G_rGCy'C
Cuts at: 0 156 764 852 1041 1049 1349 1529 1710
Size: 156 608 88 189 8 300 180 181
Cuts at: 1710 1716
Size: 6
Fragments arranged by size:
608 300 189 181 180 156 88 8
6
BbeI G_GCGC'C
Cuts at: 0 1291 1716
Size: 1291 425
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 1716
Size: 212 1504
BbvCI CC'TCA_GC
Cuts at: 0 1366 1716
Size: 1366 350
BglI GCCn_nnn'nGGC
Cuts at: 0 179 759 1716
Size: 179 580 957
Fragments arranged by size:
957 580 179
BglII A'GATC_T
Cuts at: 0 335 1716
Size: 335 1381
Bme1580I G_kGCm'C
Cuts at: 0 156 521 580 764 893 998 1041 1049
Size: 156 365 59 184 129 105 43 8
Cuts at: 1049 1438 1716
Size: 389 278
Fragments arranged by size:
389 365 278 184 156 129 105 59
43 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 462 1455 1716
Size: 462 993 261
Fragments arranged by size:
993 462 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 767 785 866 1096 1240 1300 1342
Size: 346 421 18 81 230 144 60 42
Cuts at: 1342 1450 1716
Size: 108 266
Fragments arranged by size:
421 346 266 230 144 108 81 60
42 18
Bpu10I CC'TnA_GC
Cuts at: 0 1366 1538 1716
Size: 1366 172 178
Fragments arranged by size:
1366 178 172
BsaI GGTCTCn'nnnn_
Cuts at: 0 45 1716
Size: 45 1671
BsaAI yAC'GTr
Cuts at: 0 1677 1716
Size: 1677 39
BsaHI Gr'CG_yC
Cuts at: 0 1288 1716
Size: 1288 428
BsaWI w'CCGG_w
Cuts at: 0 360 1716
Size: 360 1356
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 346 347 379 382 1084 1716
Size: 343 3 1 32 3 702 632
Fragments arranged by size:
702 632 343 32 3 3 1
BseYI C'CCAG_C
Cuts at: 0 155 235 617 677 683 725 749 765
Size: 155 80 382 60 6 42 24 16
Cuts at: 765 934 950 1088 1716
Size: 169 16 138 628
Fragments arranged by size:
628 382 169 155 138 80 60 42
24 16 16 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1716
Size: 994 722
BsiEI CG_ry'CG
Cuts at: 0 299 1155 1716
Size: 299 856 561
Fragments arranged by size:
856 561 299
BsiHKAI G_wGCw'C
Cuts at: 0 264 1513 1682 1716
Size: 264 1249 169 34
Fragments arranged by size:
1249 264 169 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 23 1716
Size: 23 1693
Bsp1286I G_dGCh'C
Cuts at: 0 156 264 521 580 764 852 893 998
Size: 156 108 257 59 184 88 41 105
Cuts at: 998 1041 1049 1349 1438 1513 1529 1682 1710
Size: 43 8 300 89 75 16 153 28
Cuts at: 1710 1716
Size: 6
Fragments arranged by size:
300 257 184 156 153 108 105 89
88 75 59 43 41 28 16 8
6
BspEI T'CCGG_A
Cuts at: 0 360 1716
Size: 360 1356
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1240 1345 1716
Size: 1240 105 371
Fragments arranged by size:
1240 371 105
BsrFI r'CCGG_y
Cuts at: 0 1002 1463 1716
Size: 1002 461 253
Fragments arranged by size:
1002 461 253
BssHII G'CGCG_C
Cuts at: 0 159 1716
Size: 159 1557
BstAPI GCAn_nnn'nTGC
Cuts at: 0 259 993 1098 1716
Size: 259 734 105 618
Fragments arranged by size:
734 618 259 105
BstYI r'GATC_y
Cuts at: 0 335 859 1716
Size: 335 524 857
Fragments arranged by size:
857 524 335
Bsu36I CC'TnA_GG
Cuts at: 0 380 695 1716
Size: 380 315 1021
Fragments arranged by size:
1021 380 315
BtgI C'CryG_G
Cuts at: 0 1373 1716
Size: 1373 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1203 1716
Size: 1203 513
BtsI GCAGTG_nn'
Cuts at: 0 457 1395 1697 1716
Size: 457 938 302 19
Fragments arranged by size:
938 457 302 19
DraIII CAC_nnn'GTG
Cuts at: 0 993 1716
Size: 993 723
EaeI y'GGCC_r
Cuts at: 0 530 1152 1545 1716
Size: 530 622 393 171
Fragments arranged by size:
622 530 393 171
EagI C'GGCC_G
Cuts at: 0 1152 1716
Size: 1152 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 767 785 866 1078 1096 1240 1300
Size: 346 421 18 81 212 18 144 60
Cuts at: 1300 1342 1450 1716
Size: 42 108 266
Fragments arranged by size:
421 346 266 212 144 108 81 60
42 18 18
EcoO109I rG'GnC_Cy
Cuts at: 0 152 376 384 760 761 1037 1038 1045
Size: 152 224 8 376 1 276 1 7
Cuts at: 1045 1046 1716
Size: 1 670
Fragments arranged by size:
670 376 276 224 152 8 7 1
1 1
FspI TGC'GCA
Cuts at: 0 1247 1716
Size: 1247 469
HaeII r_GCGC'y
Cuts at: 0 1291 1716
Size: 1291 425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 432 436 464 468 1716
Size: 432 4 28 4 1248
Fragments arranged by size:
1248 432 28 4 4
HincII GTy'rAC
Cuts at: 0 1140 1716
Size: 1140 576
KasI G'GCGC_C
Cuts at: 0 1287 1716
Size: 1287 429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 1666 1716
Size: 726 940 50
Fragments arranged by size:
940 726 50
MscI TGG'CCA
Cuts at: 0 532 1716
Size: 532 1184
MslI CAynn'nnrTG
Cuts at: 0 1241 1408 1716
Size: 1241 167 308
Fragments arranged by size:
1241 308 167
MspA1I CmG'CkG
Cuts at: 0 1083 1209 1716
Size: 1083 126 507
Fragments arranged by size:
1083 507 126
NarI GG'CG_CC
Cuts at: 0 1288 1716
Size: 1288 428
NspI r_CATG'y
Cuts at: 0 1365 1716
Size: 1365 351
PasI CC'CwG_GG
Cuts at: 0 410 1033 1042 1716
Size: 410 623 9 674
Fragments arranged by size:
674 623 410 9
PfoI T'CCnGG_A
Cuts at: 0 843 1716
Size: 843 873
PmlI CAC'GTG
Cuts at: 0 1677 1716
Size: 1677 39
PspOMI G'GGCC_C
Cuts at: 0 152 760 1037 1045 1716
Size: 152 608 277 8 671
Fragments arranged by size:
671 608 277 152 8
PstI C_TGCA'G
Cuts at: 0 570 1716
Size: 570 1146
PvuI CG_AT'CG
Cuts at: 0 299 1716
Size: 299 1417
PvuII CAG'CTG
Cuts at: 0 1083 1209 1716
Size: 1083 126 507
Fragments arranged by size:
1083 507 126
SexAI A'CCwGG_T
Cuts at: 0 1019 1716
Size: 1019 697
SfcI C'TryA_G
Cuts at: 0 566 1272 1716
Size: 566 706 444
Fragments arranged by size:
706 566 444
SfoI GGC'GCC
Cuts at: 0 1289 1716
Size: 1289 427
SmaI CCC'GGG
Cuts at: 0 123 150 499 1177 1716
Size: 123 27 349 678 539
Fragments arranged by size:
678 539 349 123 27
SphI G_CATG'C
Cuts at: 0 1365 1716
Size: 1365 351
SrfI GCCC'GGGC
Cuts at: 0 1177 1716
Size: 1177 539
StuI AGG'CCT
Cuts at: 0 1384 1716
Size: 1384 332
StyI C'CwwG_G
Cuts at: 0 595 1716
Size: 595 1121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 763 1716
Size: 27 736 953
Fragments arranged by size:
953 736 27
XmaI C'CCGG_G
Cuts at: 0 121 148 497 1175 1716
Size: 121 27 349 678 541
Fragments arranged by size:
678 541 349 121 27
XmnI GAAnn'nnTTC
Cuts at: 0 73 112 209 308 1716
Size: 73 39 97 99 1408
Fragments arranged by size:
1408 99 97 73 39
Enzymes that do cut:
AarI AcuI AhdI AleI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BglI BglII Bme1580I BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI
BssHII BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI Eco57MI EcoO109I FspI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NspI PasI
PfoI PmlI PspOMI PstI PvuI PvuII SexAI SfcI
SfoI SmaI SphI SrfI StuI StyI TaqII XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmgBI
BmrI BmtI BpuEI BsaBI BsaXI BsiWI BsmI BspHI
BsrBI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I
ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmlI
SnaBI SpeI SspI SwaI TatI TstI Tth111I XbaI
XcmI XhoI ZraI