Restriction map for
pFN21AB8711
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeIII MamI
MluI MluNI MroI MscI NaeI
NarI NcoI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tsp45I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccII 156 CGCG CG^CG
AseI 900 ATTAAT AT^TAAT
AsnI 900 ATTAAT AT^TAAT
AspHI 500 GAGCAC G[AT]GC[AT]^C
BcnI 1083 CCCGG CC^[GC]GG
BglII 1130 AGATCT A^GATCT
BseRI 497 GAGGAG GAGGAG(10/8)
BsiHKAI 500 GAGCAC G[AT]GC[AT]^C
BspDI 38 ATCGAT AT^CGAT
BspLU11I 138 ACATGT A^CATGT
BstUI 156 CGCG CG^CG
ClaI 38 ATCGAT AT^CGAT
DraI 795 TTTAAA TTT^AAA
EaeI 1197 CGGCCA [CT]^GGCC[GA]
HapII 1084 CCGG C^CGG
HgaI 154 GACGC GACGC(5/10)
HinfI 934 GAATC G^A[ACGT]TC
HpaII 1084 CCGG C^CGG
MaeII 521 ACGT A^CGT
MfeI 224 CAATTG C^AATTG
MslI 503 CACTATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1181 CCGCTG C[AC]G^C[GT]G
MspI 1084 CCGG C^CGG
MunI 224 CAATTG C^AATTG
MvnI 156 CGCG CG^CG
NciI 1083 CCCGG CC^[GC]GG
NdeI 779 CATATG CA^TATG
NheI 687 GCTAGC G^CTAGC
PflMI 411 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 900 ATTAAT AT^TAAT
SphI 1006 GCATGC GCATG^C
SspI 83 AATATT AAT^ATT
TfiI 934 GAATC G^A[AT]TC
Van91I 411 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1220 CCACTATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 283 AAATTT [GA]^AATT[CT]
784 GAATTT
AflIII 138 ACATGT A^C[GA][CT]GT
521 ACGTGT
ApoI 283 AAATTT [GA]^AATT[CT]
784 GAATTT
BmyI 500 GAGCAC G[AGT]GC[ACT]^C
1216 GTGCCC
BsgI 480 GTGCAG GTGCAG(16/14)
883 GTGCAG
BsmBI 91 GAGACG CGTCTC(1/5)
1193 GAGACG
Bsp1286I 500 GAGCAC G[AGT]GC[ACT]^C
1216 GTGCCC
BspMI 1112 ACCTGC ACCTGC(4/8)
1329 ACCTGC
BstYI 645 GGATCT [GA]^GATC[CT]
1130 AGATCT
CfoI 591 GCGC GCG^C
927 GCGC
HhaI 591 GCGC GCG^C
927 GCGC
HinP1I 591 GCGC G^CGC
927 GCGC
HphI 462 GGTGA GGTGA(8/7)
408 TCACC
MflI 645 GGATCT [GA]^GATC[CT]
1130 AGATCT
PpuMI 1014 AGGACCC [GA]G^G[AT]CC[CT]
1078 GGGACCC
SmlI 47 CTCAAG C^T[CT][GA]AG
260 CTCAAG
StuI 101 AGGCCT AGG^CCT
1313 AGGCCT
XhoII 645 GGATCT [GA]^GATC[CT]
1130 AGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII ItaI Ksp632I
MaeI MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I