Restriction map for pFN21AB8711
 (Circular) (Six-base) MAPSORT of: pFN21AB8711.seq  Check: 405  from: 1  to: 6151



pFN21AB8711  6151 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:35  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3961     278 
   Size:         53      83     186     936    2425    2468

  Fragments arranged by size: 

                2468    2425     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3494    1937 
   Size:       1557    4594
 
Acc65I G'GTAC_C

Cuts at:   3472    3472 
   Size:       6151
 
AclI AA'CG_TT

Cuts at:   1563    4278    4651    1563 
   Size:       2715     373    3063

  Fragments arranged by size: 

                3063    2715     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2979    3117    4214    5598    5866    1632 
   Size:       1347     138    1097    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1347    1097     268     138
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5954
 
AflIII A'CryG_T

Cuts at:   1203    2143    2526    5050    6027    1203 
   Size:        940     383    2524     977    1327

  Fragments arranged by size: 

                2524    1327     977     940     383
 
AgeI A'CCGG_T

Cuts at:   4982    4982 
   Size:       6151
 
AhdI GACnn_n'nnGTC

Cuts at:   4880    4880 
   Size:       6151
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6151
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6151
 
AlwNI CAG_nnn'CTG

Cuts at:   3894    5466    3894 
   Size:       1572    4579
 
ApaLI G'TGCA_C

Cuts at:   4208    5364    4208 
   Size:       1156    4995
 
ApoI r'AATT_y

Cuts at:   1493    2288    2789    3460    3686    3838    5947    1493 
   Size:        795     501     671     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     795     671     501     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2906    4705     160 
   Size:        872    1874    1799    1606

  Fragments arranged by size: 

                1874    1799    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6151
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3476    1870 
   Size:         73    1533    4545

  Fragments arranged by size: 

                4545    1533      73
 
BamHI G'GATC_C

Cuts at:   3481    3848    3481 
   Size:        367    5784
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3472    4927     618 
   Size:        325     217     627    1685    1455    1842

  Fragments arranged by size: 

                1842    1685    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3470    1391 
   Size:       2079    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5180
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4342    4376    1903 
   Size:         34    2405      34    3678

  Fragments arranged by size: 

                3678    2405      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4043    5253    4043 
   Size:       1210    4941
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6151
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4762     136 
   Size:        107     122      71     631     605    3090    1525

  Fragments arranged by size: 

                3090    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   3135    6146    3135 
   Size:       3011    3140
 
BlpI GC'TnA_GC

Cuts at:   3568    3568 
   Size:       6151
 
Bme1580I G_kGCm'C

Cuts at:   1790    3225    4212    5368    1790 
   Size:       1435     987    1156    2573

  Fragments arranged by size: 

                2573    1435    1156     987
 
BmgBI CAC'GTC

Cuts at:   1318    2527    1318 
   Size:       1209    4942
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2291    3882    4840 
   Size:        416      53      83     399      24     874    1591     958

Cuts at:   4840     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1591     958     874     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    2696    1056 
   Size:       1640    4511
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4811    1239 
   Size:       3572    2579
 
Bpu10I CC'TnA_GC

Cuts at:   3016    3268    3339    3016 
   Size:        252      71    5828

  Fragments arranged by size: 

                5828     252      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2037    2250    4276    5141    5439    2037 
   Size:        213    2026     865     298    2749

  Fragments arranged by size: 

                2749    2026     865     298     213
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4814     882 
   Size:        617    3315    2219

  Fragments arranged by size: 

                3315    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3942     493 
   Size:        711    2738    2702

  Fragments arranged by size: 

                2738    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6145    6145 
   Size:       6151
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3958    4340    4975     275 
   Size:         53      83     186     936    2425     382     635    1451

  Fragments arranged by size: 

                2425    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4584    4982    5256    5403    5814    4584 
   Size:        398     274     147     411    4921

  Fragments arranged by size: 

                4921     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5926

  Fragments arranged by size: 

                5926     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2517    1186 
   Size:       1331    4820
 
BseYI C'CCAG_C

Cuts at:   2373    5354    5850    2373 
   Size:       2981     496    2674

  Fragments arranged by size: 

                2981    2674     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2506    2909    1163 
   Size:        573     770     403    4405

  Fragments arranged by size: 

                4405     770     573     403
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3601    4362    5390    1540 
   Size:        275     186    1600     761    1028    2301

  Fragments arranged by size: 

                2301    1600    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2509    4212    4297    5368    2509 
   Size:       1703      85    1071    3292

  Fragments arranged by size: 

                3292    1703    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3662    3755    3662 
   Size:         93    6058
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2090    3192    2090 
   Size:       1102    5049
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2509    3225    3470    4212    4297    5368    1391 
   Size:        399     719     716     245     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     719     716     399     245      85
            
 
BspEI T'CCGG_A

Cuts at:   5814    5814 
   Size:       6151
 
BspHI T'CATG_A

Cuts at:   4040    5770    4040 
   Size:       1730    4421
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3126    3343    3506     844 
   Size:       2282     217     163    3489

  Fragments arranged by size: 

                3489    2282     217     163
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4038    1394 
   Size:        429    2215    3507

  Fragments arranged by size: 

                3507    2215     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4646    4820      65 
   Size:       1183    3398     174    1396

  Fragments arranged by size: 

                3398    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4795    4982    1700 
   Size:        110    2985     187    2869

  Fragments arranged by size: 

                2985    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6151
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6151
 
BssSI C'ACGA_G

Cuts at:   4211    5223    4211 
   Size:       1012    5139
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6151
 
BstBI TT'CG_AA

Cuts at:   4955    4955 
   Size:       6151
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6151
 
BstYI r'GATC_y

Cuts at:   1972    2650    3135    3481    3848    4233    4250    5702    5788 
   Size:        678     485     346     367     385      17    1452      86

Cuts at:   5788    5800    6146    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     678     485     385     367     346     346
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5599
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5412

  Fragments arranged by size: 

                5412     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3667    4461    4481    3667 
   Size:        794      20    5337

  Fragments arranged by size: 

                5337     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2044    1554 
   Size:        490    5661
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6116
 
DraI TTT'AAA

Cuts at:   2802    3455    2802 
   Size:        653    5498
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5158     809 
   Size:        507    3842    1802

  Fragments arranged by size: 

                3842    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3202    3598    4487 
   Size:         54    1425      50     275      18    1372     396     889

Cuts at:   4487    6116       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1372     889     396     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3598    1537 
   Size:        275    1786    4090

  Fragments arranged by size: 

                4090    1786     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2838    3153    3380    4081    1353 
   Size:       1485     315     227     701    3423

  Fragments arranged by size: 

                3423    1485     701     315     227
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4738    5112    5258     387 
   Size:       1064     138    3149     374     146    1280

  Fragments arranged by size: 

                3149    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2979    3117    4214    4811    5598    5866    1239 
   Size:        393    1347     138    1097     597     787     268    1524

  Fragments arranged by size: 

                1524    1347    1097     787     597     393     268     138
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2356    3020    3084    2356 
   Size:        664      64    5423

  Fragments arranged by size: 

                5423     664      64
 
EcoRI G'AATT_C

Cuts at:   3460    3838    3460 
   Size:        378    5773
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2125    2615    2669    2125 
   Size:        490      54    5607

  Fragments arranged by size: 

                5607     490      54
 
FspI TGC'GCA

Cuts at:   4657    4657 
   Size:       6151
 
HaeII r_GCGC'y

Cuts at:   1678    5298    1678 
   Size:       3620    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2194    2226    2661    2693    2981 
   Size:         32     135      32     622      32     435      32     288

Cuts at:   2981    3013    3032    3064    3380    3412    4792    4824    4866 
   Size:         32      19      32     316      32    1380      32      42

Cuts at:   4866    4898    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1380     622     435     316     288     135      42
                  32      32      32      32      32      32      32      32
                  32      19
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3495    3741     669 
   Size:        988     281    1557     246    3079

  Fragments arranged by size: 

                3079    1557     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6151
 
HpaI GTT'AAC

Cuts at:   3741    3741 
   Size:       6151
 
KpnI G_GTAC'C

Cuts at:   3476    3476 
   Size:       6151
 
MfeI C'AATT_G

Cuts at:   2229    3750    2229 
   Size:       1521    4630
 
MluI A'CGCG_T

Cuts at:   6027    6027 
   Size:       6151
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5265    5449    1305 
   Size:       3960     184    2007

  Fragments arranged by size: 

                3960    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4329

  Fragments arranged by size: 

                4329    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2512    4109    4468    4627     518 
   Size:        648    1346    1597     359     159    2042

  Fragments arranged by size: 

                2042    1597    1346     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3188    3567    4244    5392    5637    1606 
   Size:       1582     379     677    1148     245    2120

  Fragments arranged by size: 

                2120    1582    1148     677     379     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5599
 
NdeI CA'TA_TG

Cuts at:    387    2785     387 
   Size:       2398    3753
 
NheI G'CTAG_C

Cuts at:   1052    2692    1052 
   Size:       1640    4511
 
NotI GC'GGCC_GC

Cuts at:   3598    3598 
   Size:       6151
 
NspI r_CATG'y

Cuts at:   1132    1229    2147    2692    3015    3509    5054    1132 
   Size:         97     918     545     323     494    1545    2229

  Fragments arranged by size: 

                2229    1545     918     545     494     323      97
 
PasI CC'CwG_GG

Cuts at:   1348    2028    3235    1348 
   Size:        680    1207    4264

  Fragments arranged by size: 

                4264    1207     680
 
PciI A'CATG_T

Cuts at:   2143    5050    2143 
   Size:       2907    3244
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2422    1108 
   Size:       1314    4837
 
PmeI GTTT'AAAC

Cuts at:   3455    3455 
   Size:       6151
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3152    3184    4220    4252    5733    5765    3152 
   Size:         32    1036      32    1481      32    3538

  Fragments arranged by size: 

                3538    1481    1036      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3020    3084    3020 
   Size:         64    6087
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6151
 
PsiI TTA'TAA

Cuts at:   3721    3721 
   Size:       6151
 
PstI C_TGCA'G

Cuts at:    830    3503     830 
   Size:       2673    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6151
 
SalI G'TCGA_C

Cuts at:   1936    3493    1936 
   Size:       1557    4594
 
SanDI GG'GwC_CC

Cuts at:   3084    3084 
   Size:       6151
 
SbfI CC_TGCA'GG

Cuts at:   3503    3503 
   Size:       6151
 
ScaI AGT'ACT

Cuts at:   4399    6111    4399 
   Size:       1712    4439
 
SfcI C'TryA_G

Cuts at:    826    1045    3499    4634    5315    5506     826 
   Size:        219    2454    1135     681     191    1471

  Fragments arranged by size: 

                2454    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6151
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6151
 
SmaI CCC'GGG

Cuts at:   1872    3478    1872 
   Size:       1606    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2052    2265    4255    5156    5418     820 
   Size:        197     926     109     213    1990     901     262    1553

  Fragments arranged by size: 

                1990    1553     926     901     262     213     197     109
            
 
SnaBI TAC'GTA

Cuts at:    493    3942     493 
   Size:       3449    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6151
 
SphI G_CATG'C

Cuts at:   1132    3015    3509    1132 
   Size:       1883     494    3774

  Fragments arranged by size: 

                3774    1883     494
 
SspI AAT'ATT

Cuts at:      5      52    2090    4075       5 
   Size:         47    2038    1985    2081

  Fragments arranged by size: 

                2081    2038    1985      47
 
StuI AGG'CCT

Cuts at:   1517    2108    3320    1517 
   Size:        591    1212    4348

  Fragments arranged by size: 

                4348    1212     591
 
StyI C'CwwG_G

Cuts at:    513    1065    2302    3153    3252    3590     513 
   Size:        552    1237     851      99     338    3074

  Fragments arranged by size: 

                3074    1237     851     552     338      99
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4178    4195    4348    4533    4978     325 
   Size:       1255    2598      17     153     185     445    1498

  Fragments arranged by size: 

                2598    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2233    2873    4397 
   Size:        274      80      33      51    1443     256     640    1524

Cuts at:   4397    6109      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1524    1443     640     274     256     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6111    6143    6111 
   Size:         32    6119
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6151
 
XbaI T'CTAG_A

Cuts at:   3487    3487 
   Size:       6151
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3232    3232 
   Size:       6151
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6151
 
XmaI C'CCGG_G

Cuts at:   1870    3476    1870 
   Size:       1606    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4280    4280 
   Size:       6151
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3959     276 
   Size:         53      83     186     936    2425    2468

  Fragments arranged by size: 

                2468    2425     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
     SalI    SanDI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI
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