################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BcnI BfaI BfrI BglII BlnI BpmI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcsI 151 GAATTT [GA]^AATT[CT] AhdI 366 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 3 GATCC GGATC(4/5) AlwNI 644 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 334 CCTGAGG CC^T[ACGT]AGG ApoI 151 GAATTT [GA]^AATT[CT] AspEI 366 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 135 TGGCCA TGG^CCA BbvCI 544 GCTGAGG CCTCAGC(-5/-2) BbvI 316 GCTGC GCAGC(8/12) BclI 498 TGATCA T^GATCA BglI 680 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 438 GCTGAGC GC^T[ACGT]AGC Bpu1102I 438 GCTGAGC GC^T[ACGT]AGC BsaAI 175 TACGTG [CT]AC^GT[GA] BsaI 475 GAGACC GGTCTC(1/5) BsiYI 58 CCCTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 58 CCCTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 128 GTCCC GGGAC(10/14) BsrDI 443 GCAATG GCAATG(2/0) BssSI 682 CTCGTG CACGAG(-5/-1) Bsu36I 334 CCTGAGG CC^T[ACGT]AGG Cac8I 722 GCCTGC GC[ACGT]^[ACGT]GC CelII 438 GCTGAGC GC^T[ACGT]AGC CfoI 659 GCGC GCG^C EaeI 135 TGGCCA [CT]^GGCC[GA] Eam1105I 366 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 593 CTCTTC CTCTTC(1/4) Eco81I 334 CCTGAGG CC^T[ACGT]AGG EcoT14I 750 CCAAGG C^C[AT][AT]GG FauI 240 CCCGC CCCGC(4/6) FbaI 498 TGATCA T^GATCA Fnu4HI 316 GCTGC GC^[ACGT]GC HhaI 659 GCGC GCG^C HinP1I 659 GCGC G^CGC HincII 424 GTCAAC GT[CT]^[GA]AC HindII 424 GTCAAC GT[CT]^[GA]AC HinfI 618 GATTC G^A[ACGT]TC ItaI 316 GCTGC GC^[ACGT]GC Ksp632I 593 CTCTTC CTCTTC(1/4) MluNI 135 TGGCCA TGG^CCA MscI 135 TGGCCA TGG^CCA MseI 296 TTAA T^TAA MspA1I 314 CCGCTG C[AC]G^C[GT]G MwoI 680 GCCTCGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflFI 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 648 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 637 GAGCTC GAGCT^C SfaNI 21 GATGC GCATC(5/9) StuI 720 AGGCCT AGG^CCT StyI 750 CCAAGG C^C[AT][AT]GG TfiI 618 GATTC G^A[AT]TC Tru9I 296 TTAA T^TAA TseI 316 GCTGC G^C[AT]GC Tth111I 85 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 147 CCATGAATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 241 CCGC CCGC(-3/-1) 314 CCGC AfaI 114 GTAC GT^AC 141 GTAC ApaI 29 GGGCCC GGGCC^C 754 GGGCCC AspHI 571 GAGCAC G[AT]GC[AT]^C 637 GAGCTC AvaII 127 GGTCC G^G[AT]CC 180 GGACC BbsI 70 GAAGAC GAAGAC(2/6) 706 GTCTTC BpuAI 70 GAAGAC GAAGAC(2/6) 706 GTCTTC BseRI 532 CTCCTC GAGGAG(10/8) 547 GAGGAG BsiHKAI 571 GAGCAC G[AT]GC[AT]^C 637 GAGCTC BstF5I 384 CATCC GGATG(2/0) 714 CATCC FokI 384 CATCC GGATG(9/13) 714 CATCC HphI 67 GGTGA GGTGA(8/7) 527 TCACC MaeII 89 ACGT A^CGT 176 ACGT MslI 45 CACCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 690 CATTGTGGTG NlaIII 148 CATG CATG^ 434 CATG RsaI 114 GTAC GT^AC 141 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AluI BanII BmyI BsaJI BsmAI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII HaeIII MaeIII MboI MboII MnlI MvaI NdeII NlaIV Sau3AI Sau96I ScrFI Tsp45I Tsp509I TspRI