(Circular) (Six-base) MAPSORT of: pFN21AB6604.seq Check: 106 from: 1 to: 5998 pFN21AB6604 5998 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2080 2320 3078 3275 4061 5445 5713 1632 Size: 448 240 758 197 786 1384 268 1917 Fragments arranged by size: 1917 1384 786 758 448 268 240 197 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3225 1184 Size: 2041 3957 AlwNI CAG_nnn'CTG Cuts at: 3148 3741 5313 3148 Size: 593 1572 3833 Fragments arranged by size: 3833 1572 593 ApaI G_GGCC'C Cuts at: 2298 2351 2814 2298 Size: 53 463 5482 Fragments arranged by size: 5482 463 53 ApaLI G'TGCA_C Cuts at: 2114 4055 5211 2114 Size: 1941 1156 2901 Fragments arranged by size: 2901 1941 1156 ApoI r'AATT_y Cuts at: 1493 2438 2522 3307 3533 3685 5794 1493 Size: 945 84 785 226 152 2109 1697 Fragments arranged by size: 2109 1697 945 785 226 152 84 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 2119 3323 1870 Size: 73 176 1204 4545 Fragments arranged by size: 4545 1204 176 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2629 2662 2629 Size: 33 5965 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 2059 2136 2239 3175 Size: 325 217 627 245 27 77 103 936 Cuts at: 3175 3319 4774 618 Size: 144 1455 1842 Fragments arranged by size: 1842 1455 936 627 325 245 217 144 103 77 27 BanII G_rGCy'C Cuts at: 1391 2298 2351 2814 3317 1391 Size: 907 53 463 503 4072 Fragments arranged by size: 4072 907 503 463 53 BbeI G_GCGC'C Cuts at: 2036 2140 2036 Size: 104 5894 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2326 928 Size: 971 427 4600 Fragments arranged by size: 4600 971 427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4189 4223 1903 Size: 34 50 34 2168 34 3678 Fragments arranged by size: 3678 2168 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2957 4609 136 Size: 107 122 71 631 605 1285 1652 1525 Fragments arranged by size: 1652 1525 1285 631 605 122 107 71 BglII A'GATC_T Cuts at: 5993 5993 Size: 5998 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2062 2118 2298 2351 2814 3180 4059 5215 Size: 272 56 180 53 463 366 879 1156 Cuts at: 5215 1790 Size: 2573 Fragments arranged by size: 2573 1156 879 463 366 272 180 56 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2716 3729 4687 Size: 416 53 83 399 24 1299 1013 958 Cuts at: 4687 442 Size: 1753 Fragments arranged by size: 1753 1299 1013 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2134 2197 2134 Size: 63 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2582 4658 1239 Size: 965 378 2076 2579 Fragments arranged by size: 2579 2076 965 378 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4123 4988 5286 4123 Size: 865 298 4835 Fragments arranged by size: 4835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3018 4661 882 Size: 617 1519 1643 2219 Fragments arranged by size: 2219 1643 1519 617 BsaAI yAC'GTr Cuts at: 493 1204 2011 3789 493 Size: 711 807 1778 2702 Fragments arranged by size: 2702 1778 807 711 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 2137 2391 2921 Size: 53 83 186 936 500 104 254 530 Cuts at: 2921 3805 4187 4822 275 Size: 884 382 635 1451 Fragments arranged by size: 1451 936 884 635 530 500 382 254 186 104 83 53 BsaWI w'CCGG_w Cuts at: 2472 2526 2574 2877 4431 4829 5103 5250 5661 Size: 54 48 303 1554 398 274 147 411 Cuts at: 5661 2472 Size: 2809 Fragments arranged by size: 2809 1554 411 398 303 274 147 54 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5773 Fragments arranged by size: 5773 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2678 1186 Size: 1111 381 4506 Fragments arranged by size: 4506 1111 381 BseYI C'CCAG_C Cuts at: 5201 5697 5201 Size: 496 5502 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3161 1163 Size: 573 1425 4000 Fragments arranged by size: 4000 1425 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2598 2607 4059 4144 5215 2118 Size: 480 9 1452 85 1071 2901 Fragments arranged by size: 2901 1452 1071 480 85 9 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 BsmBI CGTCTCn'nnnn_ Cuts at: 2561 2747 2561 Size: 186 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2118 2298 2351 2598 2607 2814 Size: 399 272 56 180 53 247 9 207 Cuts at: 2814 3180 3317 4059 4144 5215 1391 Size: 366 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 272 247 207 180 137 85 56 53 9 BspEI T'CCGG_A Cuts at: 2526 2574 5661 2526 Size: 48 3087 2863 Fragments arranged by size: 3087 2863 48 BspHI T'CATG_A Cuts at: 2403 3887 5617 2403 Size: 1484 1730 2784 Fragments arranged by size: 2784 1730 1484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3353 844 Size: 2509 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2155 2913 3885 1394 Size: 429 332 758 972 3507 Fragments arranged by size: 3507 972 758 429 332 BsrDI GCAATG_nn' Cuts at: 65 1248 4493 4667 65 Size: 1183 3245 174 1396 Fragments arranged by size: 3245 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2133 4642 4829 1700 Size: 110 323 2509 187 2869 Fragments arranged by size: 2869 2509 323 187 110 BsrGI T'GTAC_A Cuts at: 96 2262 96 Size: 2166 3832 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 2420 3002 4058 5070 2420 Size: 582 1056 1012 3348 Fragments arranged by size: 3348 1056 1012 582 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5998 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2278 2278 Size: 5998 BstYI r'GATC_y Cuts at: 1972 2175 2490 2529 2623 3328 3695 4080 4097 Size: 203 315 39 94 705 367 385 17 Cuts at: 4097 5549 5635 5647 5993 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 705 385 367 346 315 203 94 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2271 2720 2853 2883 513 Size: 552 1206 449 133 30 3628 Fragments arranged by size: 3628 1206 552 449 133 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3034 487 Size: 683 56 1808 3451 Fragments arranged by size: 3451 1808 683 56 BtsI GCAGTG_nn' Cuts at: 2018 3514 4308 4328 2018 Size: 1496 794 20 3688 Fragments arranged by size: 3688 1496 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DraIII CAC_nnn'GTG Cuts at: 2710 2710 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2302 2358 Size: 54 1425 50 275 18 258 214 56 Cuts at: 2358 2949 3445 4334 5963 8 Size: 591 496 889 1629 43 Fragments arranged by size: 1629 1425 889 591 496 275 258 214 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2633 3928 1353 Size: 1280 1295 3423 Fragments arranged by size: 3423 1295 1280 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2071 4585 4959 5105 387 Size: 1064 138 482 2514 374 146 1280 Fragments arranged by size: 2514 1280 1064 482 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2204 2320 2582 3078 3275 4061 Size: 393 448 124 116 262 496 197 786 Cuts at: 4061 4658 5445 5713 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 786 597 496 448 393 268 262 197 124 116 EcoO109I rG'GnC_Cy Cuts at: 2347 2348 2811 2347 Size: 1 463 5534 Fragments arranged by size: 5534 463 1 EcoRI G'AATT_C Cuts at: 2438 2522 3307 3685 2438 Size: 84 785 378 4751 Fragments arranged by size: 4751 785 378 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2350 2458 2845 2350 Size: 108 387 5503 Fragments arranged by size: 5503 387 108 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 2036 2054 2140 5145 1678 Size: 358 18 86 3005 2531 Fragments arranged by size: 3005 2531 358 86 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3224 3256 4639 4671 4713 Size: 32 135 32 1652 32 1383 32 42 Cuts at: 4713 4745 1373 Size: 32 2626 Fragments arranged by size: 2626 1652 1383 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2416 3342 3588 669 Size: 988 281 478 926 246 3079 Fragments arranged by size: 3079 988 926 478 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KasI G'GCGC_C Cuts at: 2032 2136 2032 Size: 104 5894 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3267 5112 5296 1305 Size: 1962 1845 184 2007 Fragments arranged by size: 2007 1962 1845 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 2951 10 Size: 54 1425 343 528 591 3057 Fragments arranged by size: 3057 1425 591 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2249 2276 2402 3956 4315 4474 Size: 648 843 240 27 126 1554 359 159 Cuts at: 4474 518 Size: 2042 Fragments arranged by size: 2042 1554 843 648 359 240 159 126 27 MspA1I CmG'CkG Cuts at: 1606 2722 3414 4091 5239 5484 1606 Size: 1116 692 677 1148 245 2120 Fragments arranged by size: 2120 1148 1116 692 677 245 NarI GG'CG_CC Cuts at: 2033 2137 2033 Size: 104 5894 NcoI C'CATG_G Cuts at: 513 1065 2271 513 Size: 552 1206 4240 Fragments arranged by size: 4240 1206 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 3356 4901 1132 Size: 97 2127 1545 2229 Fragments arranged by size: 2229 2127 1545 97 PasI CC'CwG_GG Cuts at: 1348 2344 1348 Size: 996 5002 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 2710 1108 Size: 1602 4396 PfoI T'CCnGG_A Cuts at: 2547 2598 2547 Size: 51 5947 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PmlI CAC'GTG Cuts at: 2011 2011 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3149 1613 Size: 1536 4462 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PspOMI G'GGCC_C Cuts at: 2294 2347 2810 2294 Size: 53 463 5482 Fragments arranged by size: 5482 463 53 PspXI vC'TCGA_Gb Cuts at: 2119 2119 Size: 5998 PstI C_TGCA'G Cuts at: 830 2742 3350 830 Size: 1912 608 3478 Fragments arranged by size: 3478 1912 608 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 SacII CC_GC'GG Cuts at: 2723 2723 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 2738 3346 4481 5162 5353 826 Size: 219 1693 608 1135 681 191 1471 Fragments arranged by size: 1693 1471 1135 681 608 219 191 SfoI GGC'GCC Cuts at: 2034 2138 2034 Size: 104 5894 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 2133 1700 Size: 433 5565 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2119 4102 5003 5265 820 Size: 197 926 176 1983 901 262 1553 Fragments arranged by size: 1983 1553 926 901 262 197 176 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 2990 1517 Size: 1473 4525 StyI C'CwwG_G Cuts at: 513 1065 2271 3437 513 Size: 552 1206 1166 3074 Fragments arranged by size: 3074 1206 1166 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2257 2463 2698 4025 4042 4195 4380 Size: 1255 677 206 235 1327 17 153 185 Cuts at: 4380 4825 325 Size: 445 1498 Fragments arranged by size: 1498 1327 1255 677 445 235 206 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2262 4244 5956 Size: 274 80 33 51 1443 285 1982 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 1982 1712 1443 285 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2052 2084 5958 5990 2052 Size: 32 3874 32 2060 Fragments arranged by size: 3874 2060 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2399 1316 Size: 1083 4915 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2251 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 2119 1943 Size: 176 5822 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 4127 4127 Size: 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI